Restriction map for
pF1KB7622
(Circular) (Six-base) MAPSORT of: pF1KB7622.seq Check: 3466 from: 1 to: 4217
pF1KB7622 4217 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 540 1255 540
Size: 715 3502
Acc65I G'GTAC_C
Cuts at: 1233 1233
Size: 4217
AclI AA'CG_TT
Cuts at: 4057 4057
Size: 4217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 870 2042 2474 3221 3489 713
Size: 157 1172 432 747 268 1441
Fragments arranged by size:
1441 1172 747 432 268 157
AflIII A'CryG_T
Cuts at: 1569 2673 3650 1569
Size: 1104 977 2136
Fragments arranged by size:
2136 1104 977
AgeI A'CCGG_T
Cuts at: 525 2605 525
Size: 2080 2137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 425 425
Size: 4217
AlwNI CAG_nnn'CTG
Cuts at: 1522 3089 1522
Size: 1567 2650
ApaI G_GGCC'C
Cuts at: 305 305
Size: 4217
ApaLI G'TGCA_C
Cuts at: 2987 2987
Size: 4217
ApoI r'AATT_y
Cuts at: 173 782 920 1062 1221 3570 3826 173
Size: 609 138 142 159 2349 256 564
Fragments arranged by size:
2349 609 564 256 159 142 138
AseI AT'TA_AT
Cuts at: 20 1076 20
Size: 1056 3161
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
AvaI C'yCGr_G
Cuts at: 1237 1237
Size: 4217
BamHI G'GATC_C
Cuts at: 198 1242 198
Size: 1044 3173
BanI G'GyrC_C
Cuts at: 1233 1647 1895 1930 1233
Size: 414 248 35 3520
Fragments arranged by size:
3520 414 248 35
BanII G_rGCy'C
Cuts at: 305 656 898 1231 2261 305
Size: 351 242 333 1030 2261
Fragments arranged by size:
2261 1030 351 333 242
BbeI G_GCGC'C
Cuts at: 1651 1899 1651
Size: 248 3969
BbsI GAAGACnn'nnnn_
Cuts at: 572 1179 572
Size: 607 3610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 317 351 317
Size: 34 4183
BciVI GTATCCnnnnn_n'
Cuts at: 235 277 2115 2876 4217 235
Size: 42 1838 761 1341 235
Fragments arranged by size:
1838 1341 761 235 42
BclI T'GATC_A
Cuts at: 1737 1737
Size: 4217
BglII A'GATC_T
Cuts at: 1732 3769 1732
Size: 2037 2180
BlpI GC'TnA_GC
Cuts at: 897 1329 897
Size: 432 3785
Bme1580I G_kGCm'C
Cuts at: 305 1842 1935 2991 305
Size: 1537 93 1056 1531
Fragments arranged by size:
1537 1531 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1106 1429 1510 1845 3989 1106
Size: 323 81 335 2144 1334
Fragments arranged by size:
2144 1334 335 323 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2584 2584
Size: 4217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 418 508 1034 140
Size: 278 90 526 3323
Fragments arranged by size:
3323 526 278 90
Bpu10I CC'TnA_GC
Cuts at: 349 590 349
Size: 241 3976
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 619 1393 2272 2764 3062 563
Size: 56 774 879 492 298 1718
Fragments arranged by size:
1718 879 774 492 298 56
BsaI GGTCTCn'nnnn_
Cuts at: 3921 3921
Size: 4217
BsaAI yAC'GTr
Cuts at: 1570 1570
Size: 4217
BsaBI GATnn'nnATC
Cuts at: 6 452 1731 3768 6
Size: 446 1279 2037 455
Fragments arranged by size:
2037 1279 455 446
BsaHI Gr'CG_yC
Cuts at: 1648 1896 2598 4094 1648
Size: 248 702 1496 1771
Fragments arranged by size:
1771 1496 702 248
BsaWI w'CCGG_w
Cuts at: 525 1406 1927 2605 2879 3026 3437 525
Size: 881 521 678 274 147 411 1305
Fragments arranged by size:
1305 881 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 484 208
Size: 276 3941
BseYI C'CCAG_C
Cuts at: 1642 2400 2977 3473 1642
Size: 758 577 496 2386
Fragments arranged by size:
2386 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 98
Size: 4217
BsiEI CG_ry'CG
Cuts at: 104 1759 1805 3013 104
Size: 1655 46 1208 1308
Fragments arranged by size:
1655 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 656 705 1231 2009 2199 2991 656
Size: 49 526 778 190 792 1882
Fragments arranged by size:
1882 792 778 526 190 49
BsmI GAATG_Cn'
Cuts at: 967 1120 967
Size: 153 4064
BsmBI CGTCTCn'nnnn_
Cuts at: 352 352
Size: 4217
Bsp1286I G_dGCh'C
Cuts at: 305 656 705 898 1231 1842 1935 2009 2199
Size: 351 49 193 333 611 93 74 190
Cuts at: 2199 2261 2991 305
Size: 62 730 1531
Fragments arranged by size:
1531 730 611 351 333 193 190 93
74 62 49
BspEI T'CCGG_A
Cuts at: 3437 3437
Size: 4217
BspHI T'CATG_A
Cuts at: 3393 3393
Size: 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 809 1267 1481 1783 2164 809
Size: 458 214 302 381 2862
Fragments arranged by size:
2862 458 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2509 2563 4047 4212 50
Size: 2459 54 1484 165 55
Fragments arranged by size:
2459 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2129 2129
Size: 4217
BsrFI r'CCGG_y
Cuts at: 525 2215 2396 2605 525
Size: 1690 181 209 2137
Fragments arranged by size:
2137 1690 209 181
BssSI C'ACGA_G
Cuts at: 2488 2846 2488
Size: 358 3859
BstAPI GCAn_nnn'nTGC
Cuts at: 1594 1594
Size: 4217
BstBI TT'CG_AA
Cuts at: 2578 2578
Size: 4217
BstYI r'GATC_y
Cuts at: 1 198 1242 1709 1732 2067 2313 3325 3411
Size: 197 1044 467 23 335 246 1012 86
Cuts at: 3411 3423 3769 1
Size: 12 346 449
Fragments arranged by size:
1044 1012 467 449 346 335 246 197
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 664 664
Size: 4217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2167 2348 2511 2167
Size: 181 163 3873
Fragments arranged by size:
3873 181 163
BtsI GCAGTG_nn'
Cuts at: 482 1460 482
Size: 978 3239
DraI TTT'AAA
Cuts at: 789 1216 789
Size: 427 3790
DrdI GACnn_nn'nnGTC
Cuts at: 1923 2781 1923
Size: 858 3359
EaeI y'GGCC_r
Cuts at: 1802 1976 2367 2394 3739 1802
Size: 174 391 27 1345 2280
Fragments arranged by size:
2280 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1802 1802
Size: 4217
EarI CTCTTCn'nnn_
Cuts at: 158 206 2240 2450 158
Size: 48 2034 210 1925
Fragments arranged by size:
2034 1925 210 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2425 2735 2881 4027 2425
Size: 310 146 1146 2615
Fragments arranged by size:
2615 1146 310 146
EcoICRI GAG'CTC
Cuts at: 654 1229 654
Size: 575 3642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 418 508 713 870 1034 2042 2474 3221
Size: 278 90 205 157 164 1008 432 747
Cuts at: 3221 3489 140
Size: 268 868
Fragments arranged by size:
1008 868 747 432 278 268 205 164
157 90
EcoO109I rG'GnC_Cy
Cuts at: 302 1356 302
Size: 1054 3163
EcoRI G'AATT_C
Cuts at: 920 1062 1221 920
Size: 142 159 3916
Fragments arranged by size:
3916 159 142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 4217
FspI TGC'GCA
Cuts at: 1998 1998
Size: 4217
HaeII r_GCGC'y
Cuts at: 1651 1899 2921 3900 1651
Size: 248 1022 979 1968
Fragments arranged by size:
1968 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 209 241 559 591 2059 2089 2091 2121 209
Size: 32 318 32 1468 30 2 30 2305
Fragments arranged by size:
2305 1468 318 32 32 30 30 2
HincII GTy'rAC
Cuts at: 541 1256 541
Size: 715 3502
KasI G'GCGC_C
Cuts at: 1647 1895 1647
Size: 248 3969
KpnI G_GTAC'C
Cuts at: 1237 1237
Size: 4217
MluI A'CGCG_T
Cuts at: 3650 3650
Size: 4217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 170 285 2888 3072 170
Size: 115 2603 184 1315
Fragments arranged by size:
2603 1315 184 115
MscI TGG'CCA
Cuts at: 1978 1978
Size: 4217
MslI CAynn'nnrTG
Cuts at: 674 863 988 2206 2333 674
Size: 189 125 1218 127 2558
Fragments arranged by size:
2558 1218 189 127 125
MspA1I CmG'CkG
Cuts at: 1328 1642 2002 3015 3260 1328
Size: 314 360 1013 245 2285
Fragments arranged by size:
2285 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2398 2398
Size: 4217
NarI GG'CG_CC
Cuts at: 1648 1896 1648
Size: 248 3969
NgoMIV G'CCGG_C
Cuts at: 2396 2396
Size: 4217
NspI r_CATG'y
Cuts at: 1270 1770 2677 1270
Size: 500 907 2810
Fragments arranged by size:
2810 907 500
PciI A'CATG_T
Cuts at: 2673 2673
Size: 4217
PflMI CCAn_nnn'nTGG
Cuts at: 463 463
Size: 4217
PmeI GTTT'AAAC
Cuts at: 1216 1216
Size: 4217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 4185
PspOMI G'GGCC_C
Cuts at: 301 301
Size: 4217
PstI C_TGCA'G
Cuts at: 1264 1949 1264
Size: 685 3532
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4217
PvuII CAG'CTG
Cuts at: 1642 2002 1642
Size: 360 3857
RsrII CG'GwC_CG
Cuts at: 2412 2412
Size: 4217
SacI G_AGCT'C
Cuts at: 656 1231 656
Size: 575 3642
SalI G'TCGA_C
Cuts at: 539 1254 539
Size: 715 3502
SapI GCTCTTCn'nnn_
Cuts at: 2240 2450 2240
Size: 210 4007
SbfI CC_TGCA'GG
Cuts at: 1264 1264
Size: 4217
ScaI AGT'ACT
Cuts at: 557 3734 557
Size: 3177 1040
SfcI C'TryA_G
Cuts at: 33 430 1260 1945 2938 3129 33
Size: 397 830 685 993 191 1121
Fragments arranged by size:
1121 993 830 685 397 191
SfoI GGC'GCC
Cuts at: 1649 1897 1649
Size: 248 3969
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
SmaI CCC'GGG
Cuts at: 1239 1239
Size: 4217
SmlI C'TyrA_G
Cuts at: 578 634 1372 2287 2779 3041 578
Size: 56 738 915 492 262 1754
Fragments arranged by size:
1754 915 738 492 262 56
SpeI A'CTAG_T
Cuts at: 67 558 67
Size: 491 3726
SphI G_CATG'C
Cuts at: 1270 1770 1270
Size: 500 3717
StyI C'CwwG_G
Cuts at: 812 1351 1705 812
Size: 539 354 3324
Fragments arranged by size:
3324 539 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 760 1800 1933 2392 2601 760
Size: 1040 133 459 209 2376
Fragments arranged by size:
2376 1040 459 209 133
TatI w'GTAC_w
Cuts at: 555 3732 555
Size: 3177 1040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 4185
Tth111I GACn'n_nGTC
Cuts at: 2014 2014
Size: 4217
XbaI T'CTAG_A
Cuts at: 1248 1248
Size: 4217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 295 751 1712 295
Size: 456 961 2800
Fragments arranged by size:
2800 961 456
XmaI C'CCGG_G
Cuts at: 1237 1237
Size: 4217
XmnI GAAnn'nnTTC
Cuts at: 173 967 1118 1190 173
Size: 794 151 72 3200
Fragments arranged by size:
3200 794 151 72
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI