Restriction map for
pF1KB7611
(Circular) (Six-base) MAPSORT of: pF1KB7611.seq Check: 3138 from: 1 to: 4019
pF1KB7611 4019 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1057 1057
Size: 4019
Acc65I G'GTAC_C
Cuts at: 1035 1035
Size: 4019
AclI AA'CG_TT
Cuts at: 3859 3859
Size: 4019
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 921 1844 2276 3023 3291 921
Size: 923 432 747 268 1649
Fragments arranged by size:
1649 923 747 432 268
AflIII A'CryG_T
Cuts at: 1371 2475 3452 1371
Size: 1104 977 1938
Fragments arranged by size:
1938 1104 977
AgeI A'CCGG_T
Cuts at: 2407 2407
Size: 4019
AlwNI CAG_nnn'CTG
Cuts at: 169 742 759 1324 2891 169
Size: 573 17 565 1567 1297
Fragments arranged by size:
1567 1297 573 565 17
ApaI G_GGCC'C
Cuts at: 750 772 750
Size: 22 3997
ApaLI G'TGCA_C
Cuts at: 952 2789 952
Size: 1837 2182
ApoI r'AATT_y
Cuts at: 1023 3372 3628 1023
Size: 2349 256 1414
Fragments arranged by size:
2349 1414 256
AscI GG'CGCG_CC
Cuts at: 447 447
Size: 4019
AseI AT'TA_AT
Cuts at: 20 20
Size: 4019
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
AvaI C'yCGr_G
Cuts at: 517 723 775 891 1039 517
Size: 206 52 116 148 3497
Fragments arranged by size:
3497 206 148 116 52
BamHI G'GATC_C
Cuts at: 1044 1044
Size: 4019
BanI G'GyrC_C
Cuts at: 510 528 702 727 1035 1449 1697 1732 510
Size: 18 174 25 308 414 248 35 2797
Fragments arranged by size:
2797 414 308 248 174 35 25 18
BanII G_rGCy'C
Cuts at: 148 750 772 1033 2063 148
Size: 602 22 261 1030 2104
Fragments arranged by size:
2104 1030 602 261 22
BbeI G_GCGC'C
Cuts at: 514 1453 1701 514
Size: 939 248 2832
Fragments arranged by size:
2832 939 248
BbsI GAAGACnn'nnnn_
Cuts at: 660 660
Size: 4019
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 373 407 373
Size: 34 3985
BciVI GTATCCnnnnn_n'
Cuts at: 1917 2678 4019 1917
Size: 761 1341 1917
Fragments arranged by size:
1917 1341 761
BclI T'GATC_A
Cuts at: 1539 1539
Size: 4019
BglI GCCn_nnn'nGGC
Cuts at: 847 847
Size: 4019
BglII A'GATC_T
Cuts at: 1534 3571 1534
Size: 2037 1982
BlpI GC'TnA_GC
Cuts at: 124 1131 124
Size: 1007 3012
Bme1580I G_kGCm'C
Cuts at: 707 730 750 772 956 1644 1737 2793 707
Size: 23 20 22 184 688 93 1056 1933
Fragments arranged by size:
1933 1056 688 184 93 23 22 20
BmrI ACTGGGnnnn_n'
Cuts at: 1231 1312 1647 3791 1231
Size: 81 335 2144 1459
Fragments arranged by size:
2144 1459 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4019
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 2074 2566 2864 1195
Size: 879 492 298 2350
Fragments arranged by size:
2350 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3723 3723
Size: 4019
BsaAI yAC'GTr
Cuts at: 1372 1372
Size: 4019
BsaBI GATnn'nnATC
Cuts at: 6 1533 3570 6
Size: 1527 2037 455
Fragments arranged by size:
2037 1527 455
BsaHI Gr'CG_yC
Cuts at: 511 1450 1698 2400 3896 511
Size: 939 248 702 1496 634
Fragments arranged by size:
1496 939 702 634 248
BsaWI w'CCGG_w
Cuts at: 1208 1729 2407 2681 2828 3239 1208
Size: 521 678 274 147 411 1988
Fragments arranged by size:
1988 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 556 400
Size: 156 3863
BseYI C'CCAG_C
Cuts at: 185 679 1444 2202 2779 3275 185
Size: 494 765 758 577 496 929
Fragments arranged by size:
929 765 758 577 496 494
BsiEI CG_ry'CG
Cuts at: 104 791 833 1561 1607 2815 104
Size: 687 42 728 46 1208 1308
Fragments arranged by size:
1308 1208 728 687 46 42
BsiHKAI G_wGCw'C
Cuts at: 200 221 379 956 1033 1811 2001 2793 200
Size: 21 158 577 77 778 190 792 1426
Fragments arranged by size:
1426 792 778 577 190 158 77 21
BsiWI C'GTAC_G
Cuts at: 563 563
Size: 4019
Bsp1286I G_dGCh'C
Cuts at: 148 200 221 379 707 730 750 772 956
Size: 52 21 158 328 23 20 22 184
Cuts at: 956 1033 1644 1737 1811 2001 2063 2793 148
Size: 77 611 93 74 190 62 730 1374
Fragments arranged by size:
1374 730 611 328 190 184 158 93
77 74 62 52 23 22 21 20
BspEI T'CCGG_A
Cuts at: 3239 3239
Size: 4019
BspHI T'CATG_A
Cuts at: 3195 3195
Size: 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1069 1283 1585 1966 1069
Size: 214 302 381 3122
Fragments arranged by size:
3122 381 302 214
BsrBI CCG'CTC
Cuts at: 50 516 600 2311 2365 3849 4014 50
Size: 466 84 1711 54 1484 165 55
Fragments arranged by size:
1711 1484 466 165 84 55 54
BsrDI GCAATG_nn'
Cuts at: 1931 1931
Size: 4019
BsrFI r'CCGG_y
Cuts at: 443 2017 2198 2407 443
Size: 1574 181 209 2055
Fragments arranged by size:
2055 1574 209 181
BssHII G'CGCG_C
Cuts at: 447 447
Size: 4019
BssSI C'ACGA_G
Cuts at: 2290 2648 2290
Size: 358 3661
BstAPI GCAn_nnn'nTGC
Cuts at: 1396 1396
Size: 4019
BstBI TT'CG_AA
Cuts at: 2380 2380
Size: 4019
BstYI r'GATC_y
Cuts at: 1 1044 1511 1534 1869 2115 3127 3213 3225
Size: 1043 467 23 335 246 1012 86 12
Cuts at: 3225 3571 1
Size: 346 449
Fragments arranged by size:
1043 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 698 713 845 998 107
Size: 591 15 132 153 3128
Fragments arranged by size:
3128 591 153 132 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 672 1969 2150 2313 672
Size: 1297 181 163 2378
Fragments arranged by size:
2378 1297 181 163
BtsI GCAGTG_nn'
Cuts at: 1262 1262
Size: 4019
DraI TTT'AAA
Cuts at: 1018 1018
Size: 4019
DrdI GACnn_nn'nnGTC
Cuts at: 1725 2583 1725
Size: 858 3161
EaeI y'GGCC_r
Cuts at: 574 788 830 899 1604 1778 2169 2196 3541
Size: 214 42 69 705 174 391 27 1345
Cuts at: 3541 574
Size: 1052
Fragments arranged by size:
1345 1052 705 391 214 174 69 42
27
EagI C'GGCC_G
Cuts at: 788 830 1604 788
Size: 42 774 3203
Fragments arranged by size:
3203 774 42
EarI CTCTTCn'nnn_
Cuts at: 864 2042 2252 864
Size: 1178 210 2631
Fragments arranged by size:
2631 1178 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 474 699 2227 2537 2683 3829 474
Size: 225 1528 310 146 1146 664
Fragments arranged by size:
1528 1146 664 310 225 146
EcoICRI GAG'CTC
Cuts at: 1031 1031
Size: 4019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 921 1844 2276 3023 3291 921
Size: 923 432 747 268 1649
Fragments arranged by size:
1649 923 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 351 412 351
Size: 61 3958
EcoO109I rG'GnC_Cy
Cuts at: 646 739 746 747 756 768 769 879 1158
Size: 93 7 1 9 12 1 110 279
Cuts at: 1158 646
Size: 3507
Fragments arranged by size:
3507 279 110 93 12 9 7 1
1
EcoRI G'AATT_C
Cuts at: 1023 1023
Size: 4019
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 4019
FspI TGC'GCA
Cuts at: 1800 1800
Size: 4019
HaeII r_GCGC'y
Cuts at: 514 1453 1701 2723 3702 514
Size: 939 248 1022 979 831
Fragments arranged by size:
1022 979 939 831 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1861 1891 1893 1923 1861
Size: 30 2 30 3957
Fragments arranged by size:
3957 30 30 2
HincII GTy'rAC
Cuts at: 639 1058 639
Size: 419 3600
KasI G'GCGC_C
Cuts at: 510 1449 1697 510
Size: 939 248 2832
Fragments arranged by size:
2832 939 248
KpnI G_GTAC'C
Cuts at: 1039 1039
Size: 4019
MluI A'CGCG_T
Cuts at: 3452 3452
Size: 4019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2690 2874 2690
Size: 184 3835
MscI TGG'CCA
Cuts at: 901 1780 901
Size: 879 3140
MslI CAynn'nnrTG
Cuts at: 2008 2135 2008
Size: 127 3892
MspA1I CmG'CkG
Cuts at: 294 715 847 949 1130 1444 1804 2817 3062
Size: 421 132 102 181 314 360 1013 245
Cuts at: 3062 294
Size: 1251
Fragments arranged by size:
1251 1013 421 360 314 245 181 132
102
NaeI GCC'GGC
Cuts at: 2200 2200
Size: 4019
NarI GG'CG_CC
Cuts at: 511 1450 1698 511
Size: 939 248 2832
Fragments arranged by size:
2832 939 248
NcoI C'CATG_G
Cuts at: 107 698 107
Size: 591 3428
NgoMIV G'CCGG_C
Cuts at: 2198 2198
Size: 4019
NspI r_CATG'y
Cuts at: 1072 1572 2479 1072
Size: 500 907 2612
Fragments arranged by size:
2612 907 500
PciI A'CATG_T
Cuts at: 2475 2475
Size: 4019
PflMI CCAn_nnn'nTGG
Cuts at: 742 742
Size: 4019
PmeI GTTT'AAAC
Cuts at: 1018 1018
Size: 4019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 3987
PpuMI rG'GwC_Cy
Cuts at: 879 879
Size: 4019
PspOMI G'GGCC_C
Cuts at: 746 768 746
Size: 22 3997
PspXI vC'TCGA_Gb
Cuts at: 891 891
Size: 4019
PstI C_TGCA'G
Cuts at: 755 1066 1751 755
Size: 311 685 3023
Fragments arranged by size:
3023 685 311
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4019
PvuII CAG'CTG
Cuts at: 1444 1804 1444
Size: 360 3659
RsrII CG'GwC_CG
Cuts at: 2214 2214
Size: 4019
SacI G_AGCT'C
Cuts at: 1033 1033
Size: 4019
SacII CC_GC'GG
Cuts at: 716 848 716
Size: 132 3887
SalI G'TCGA_C
Cuts at: 1056 1056
Size: 4019
SanDI GG'GwC_CC
Cuts at: 879 879
Size: 4019
SapI GCTCTTCn'nnn_
Cuts at: 2042 2252 2042
Size: 210 3809
SbfI CC_TGCA'GG
Cuts at: 755 1066 755
Size: 311 3708
ScaI AGT'ACT
Cuts at: 3536 3536
Size: 4019
SfcI C'TryA_G
Cuts at: 33 328 751 1062 1747 2740 2931 33
Size: 295 423 311 685 993 191 1121
Fragments arranged by size:
1121 993 685 423 311 295 191
SfoI GGC'GCC
Cuts at: 512 1451 1699 512
Size: 939 248 2832
Fragments arranged by size:
2832 939 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
SmaI CCC'GGG
Cuts at: 725 777 1041 725
Size: 52 264 3703
Fragments arranged by size:
3703 264 52
SmlI C'TyrA_G
Cuts at: 891 1174 2089 2581 2843 891
Size: 283 915 492 262 2067
Fragments arranged by size:
2067 915 492 283 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4019
SphI G_CATG'C
Cuts at: 1072 1572 1072
Size: 500 3519
StuI AGG'CCT
Cuts at: 238 238
Size: 4019
StyI C'CwwG_G
Cuts at: 107 581 698 1153 1507 107
Size: 474 117 455 354 2619
Fragments arranged by size:
2619 474 455 354 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 624 693 1602 1735 2194 2403 624
Size: 69 909 133 459 209 2240
Fragments arranged by size:
2240 909 459 209 133 69
TatI w'GTAC_w
Cuts at: 3534 3534
Size: 4019
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 3987
Tth111I GACn'n_nGTC
Cuts at: 1816 1816
Size: 4019
XbaI T'CTAG_A
Cuts at: 1050 1050
Size: 4019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1514 1514
Size: 4019
XhoI C'TCGA_G
Cuts at: 891 891
Size: 4019
XmaI C'CCGG_G
Cuts at: 723 775 1039 723
Size: 52 264 3703
Fragments arranged by size:
3703 264 52
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BpmI Bpu10I
BsaXI BsgI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XmnI ZraI