Restriction map for
pF1KB7607
(Circular) (Six-base) MAPSORT of: pF1KB7607.seq Check: 8281 from: 1 to: 4010
pF1KB7607 4010 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AatII G_ACGT'C
Cuts at: 305 305
Size: 4010
AccI GT'mk_AC
Cuts at: 1048 1048
Size: 4010
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1835 2267 3014 3282 349
Size: 1486 432 747 268 1077
Fragments arranged by size:
1486 1077 747 432 268
AflIII A'CryG_T
Cuts at: 1362 2466 3443 1362
Size: 1104 977 1929
Fragments arranged by size:
1929 1104 977
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AleI CACnn'nnGTG
Cuts at: 496 574 496
Size: 78 3932
AlwNI CAG_nnn'CTG
Cuts at: 1315 2882 1315
Size: 1567 2443
ApaI G_GGCC'C
Cuts at: 134 802 134
Size: 668 3342
ApaLI G'TGCA_C
Cuts at: 2780 2780
Size: 4010
ApoI r'AATT_y
Cuts at: 910 937 1014 3363 3619 910
Size: 27 77 2349 256 1301
Fragments arranged by size:
2349 1301 256 77 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 126 1030 126
Size: 904 3106
BamHI G'GATC_C
Cuts at: 1035 1035
Size: 4010
BanI G'GyrC_C
Cuts at: 783 883 928 1026 1440 1688 1723 783
Size: 100 45 98 414 248 35 3070
Fragments arranged by size:
3070 414 248 100 98 45 35
BanII G_rGCy'C
Cuts at: 134 601 745 802 1024 2054 134
Size: 467 144 57 222 1030 2090
Fragments arranged by size:
2090 1030 467 222 144 57
BbeI G_GCGC'C
Cuts at: 887 1444 1692 887
Size: 557 248 3205
Fragments arranged by size:
3205 557 248
BciVI GTATCCnnnnn_n'
Cuts at: 1908 2669 4010 1908
Size: 761 1341 1908
Fragments arranged by size:
1908 1341 761
BclI T'GATC_A
Cuts at: 1530 1530
Size: 4010
BglI GCCn_nnn'nGGC
Cuts at: 112 557 112
Size: 445 3565
BglII A'GATC_T
Cuts at: 998 1525 3562 998
Size: 527 2037 1446
Fragments arranged by size:
2037 1446 527
BlpI GC'TnA_GC
Cuts at: 1122 1122
Size: 4010
Bme1580I G_kGCm'C
Cuts at: 134 200 475 788 802 931 1635 1728 2784
Size: 66 275 313 14 129 704 93 1056
Cuts at: 2784 134
Size: 1360
Fragments arranged by size:
1360 1056 704 313 275 129 93 66
14
BmgBI CAC'GTC
Cuts at: 476 476
Size: 4010
BmrI ACTGGGnnnn_n'
Cuts at: 164 285 843 1222 1303 1638 3782 164
Size: 121 558 379 81 335 2144 392
Fragments arranged by size:
2144 558 392 379 335 121 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 289 615 280
Size: 9 326 3675
Fragments arranged by size:
3675 326 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2065 2557 2855 1186
Size: 879 492 298 2341
Fragments arranged by size:
2341 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 740 3714 740
Size: 2974 1036
BsaAI yAC'GTr
Cuts at: 1363 1363
Size: 4010
BsaBI GATnn'nnATC
Cuts at: 6 1524 3561 6
Size: 1518 2037 455
Fragments arranged by size:
2037 1518 455
BsaHI Gr'CG_yC
Cuts at: 302 884 1441 1689 2391 3887 302
Size: 582 557 248 702 1496 425
Fragments arranged by size:
1496 702 582 557 425 248
BsaWI w'CCGG_w
Cuts at: 374 1199 1720 2398 2672 2819 3230 374
Size: 825 521 678 274 147 411 1154
Fragments arranged by size:
1154 825 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 262 292 262
Size: 30 3980
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 331 277
Size: 54 3956
BseYI C'CCAG_C
Cuts at: 600 637 788 1435 2193 2770 3266 600
Size: 37 151 647 758 577 496 1344
Fragments arranged by size:
1344 758 647 577 496 151 37
BsiEI CG_ry'CG
Cuts at: 104 1552 1598 2806 104
Size: 1448 46 1208 1308
Fragments arranged by size:
1448 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 745 1024 1802 1992 2784 745
Size: 279 778 190 792 1971
Fragments arranged by size:
1971 792 778 279 190
BsmBI CGTCTCn'nnnn_
Cuts at: 665 665
Size: 4010
Bsp1286I G_dGCh'C
Cuts at: 134 200 475 601 745 788 802 931 1024
Size: 66 275 126 144 43 14 129 93
Cuts at: 1024 1635 1728 1802 1992 2054 2784 134
Size: 611 93 74 190 62 730 1360
Fragments arranged by size:
1360 730 611 275 190 144 129 126
93 93 74 66 62 43 14
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1060 1274 1576 1957 1060
Size: 214 302 381 3113
Fragments arranged by size:
3113 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2302 2356 3840 4005 50
Size: 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1922 1922
Size: 4010
BsrFI r'CCGG_y
Cuts at: 2008 2189 2398 2008
Size: 181 209 3620
Fragments arranged by size:
3620 209 181
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 280 1387 280
Size: 1107 2903
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstXI CCAn_nnnn'nTGG
Cuts at: 576 880 576
Size: 304 3706
BstYI r'GATC_y
Cuts at: 1 177 998 1035 1502 1525 1860 2106 3118
Size: 176 821 37 467 23 335 246 1012
Cuts at: 3118 3204 3216 3562 1
Size: 86 12 346 449
Fragments arranged by size:
1012 821 467 449 346 335 246 176
86 37 23 12
Bsu36I CC'TnA_GG
Cuts at: 555 623 651 555
Size: 68 28 3914
Fragments arranged by size:
3914 68 28
BtgI C'CryG_G
Cuts at: 569 779 830 896 923 953 569
Size: 210 51 66 27 30 3626
Fragments arranged by size:
3626 210 66 51 30 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 581 1253 581
Size: 672 3338
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 578 1595 1769 2160 2187 3532 578
Size: 1017 174 391 27 1345 1056
Fragments arranged by size:
1345 1056 1017 391 174 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 720 2033 2243 720
Size: 1313 210 2487
Fragments arranged by size:
2487 1313 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 660 714 2218 2528 2674 3820 660
Size: 54 1504 310 146 1146 850
Fragments arranged by size:
1504 1146 850 310 146 54
EcoICRI GAG'CTC
Cuts at: 743 1022 743
Size: 279 3731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 289 349 615 1835 2267 3014 3282 280
Size: 9 60 266 1220 432 747 268 1008
Fragments arranged by size:
1220 1008 747 432 268 266 60 9
EcoO109I rG'GnC_Cy
Cuts at: 130 131 798 835 918 1149 130
Size: 1 667 37 83 231 2991
Fragments arranged by size:
2991 667 231 83 37 1
EcoRI G'AATT_C
Cuts at: 910 1014 910
Size: 104 3906
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 4010
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 212 887 1444 1692 2714 3693 212
Size: 675 557 248 1022 979 529
Fragments arranged by size:
1022 979 675 557 529 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1852 1882 1884 1914 1852
Size: 30 2 30 3948
Fragments arranged by size:
3948 30 30 2
HincII GTy'rAC
Cuts at: 147 1049 147
Size: 902 3108
KasI G'GCGC_C
Cuts at: 883 1440 1688 883
Size: 557 248 3205
Fragments arranged by size:
3205 557 248
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2681 2865 2681
Size: 184 3826
MscI TGG'CCA
Cuts at: 580 1771 580
Size: 1191 2819
MslI CAynn'nnrTG
Cuts at: 496 574 1999 2126 496
Size: 78 1425 127 2380
Fragments arranged by size:
2380 1425 127 78
MspA1I CmG'CkG
Cuts at: 225 633 755 1121 1435 1795 2808 3053 225
Size: 408 122 366 314 360 1013 245 1182
Fragments arranged by size:
1182 1013 408 366 360 314 245 122
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 884 1441 1689 884
Size: 557 248 3205
Fragments arranged by size:
3205 557 248
NcoI C'CATG_G
Cuts at: 779 953 779
Size: 174 3836
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NspI r_CATG'y
Cuts at: 1063 1563 2470 1063
Size: 500 907 2603
Fragments arranged by size:
2603 907 500
PasI CC'CwG_GG
Cuts at: 430 430
Size: 4010
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PpuMI rG'GwC_Cy
Cuts at: 835 918 835
Size: 83 3927
PspOMI G'GGCC_C
Cuts at: 130 798 130
Size: 668 3342
PstI C_TGCA'G
Cuts at: 1057 1742 1057
Size: 685 3325
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 1435 1795 1435
Size: 360 3650
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 745 1024 745
Size: 279 3731
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 720 2033 2243 720
Size: 1313 210 2487
Fragments arranged by size:
2487 1313 210
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 3527 3527
Size: 4010
SfcI C'TryA_G
Cuts at: 33 531 888 1053 1738 2731 2922 33
Size: 498 357 165 685 993 191 1121
Fragments arranged by size:
1121 993 685 498 357 191 165
SfoI GGC'GCC
Cuts at: 885 1442 1690 885
Size: 557 248 3205
Fragments arranged by size:
3205 557 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 128 1032 128
Size: 904 3106
SmlI C'TyrA_G
Cuts at: 1165 2080 2572 2834 1165
Size: 915 492 262 2341
Fragments arranged by size:
2341 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
StyI C'CwwG_G
Cuts at: 152 443 779 953 1144 1498 152
Size: 291 336 174 191 354 2664
Fragments arranged by size:
2664 354 336 291 191 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1593 1726 2185 2394 1593
Size: 133 459 209 3209
Fragments arranged by size:
3209 459 209 133
TatI w'GTAC_w
Cuts at: 3525 3525
Size: 4010
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 933 965 3527 3559 933
Size: 32 2562 32 1384
Fragments arranged by size:
2562 1384 32 32
Tth111I GACn'n_nGTC
Cuts at: 1807 1807
Size: 4010
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 960 1505 960
Size: 545 3465
XmaI C'CCGG_G
Cuts at: 126 1030 126
Size: 904 3106
ZraI GAC'GTC
Cuts at: 303 303
Size: 4010
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsgI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI