Restriction map for
pF1KB7595
(Circular) (Six-base) MAPSORT of: pF1KB7595.seq Check: 1997 from: 1 to: 3812
pF1KB7595 3812 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 785 850 785
Size: 65 3747
Acc65I G'GTAC_C
Cuts at: 828 828
Size: 3812
AclI AA'CG_TT
Cuts at: 3652 3652
Size: 3812
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1637 2069 2816 3084 1637
Size: 432 747 268 2365
Fragments arranged by size:
2365 747 432 268
AfeI AGC'GCT
Cuts at: 735 735
Size: 3812
AflIII A'CryG_T
Cuts at: 1164 2268 3245 1164
Size: 1104 977 1731
Fragments arranged by size:
1731 1104 977
AgeI A'CCGG_T
Cuts at: 2200 2200
Size: 3812
AleI CACnn'nnGTG
Cuts at: 553 553
Size: 3812
AlwNI CAG_nnn'CTG
Cuts at: 420 1117 2684 420
Size: 697 1567 1548
Fragments arranged by size:
1567 1548 697
ApaI G_GGCC'C
Cuts at: 179 179
Size: 3812
ApaLI G'TGCA_C
Cuts at: 2582 2582
Size: 3812
ApoI r'AATT_y
Cuts at: 816 3165 3421 816
Size: 2349 256 1207
Fragments arranged by size:
2349 1207 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3812
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
AvaI C'yCGr_G
Cuts at: 357 832 357
Size: 475 3337
BamHI G'GATC_C
Cuts at: 837 837
Size: 3812
BanI G'GyrC_C
Cuts at: 235 483 828 1242 1490 1525 235
Size: 248 345 414 248 35 2522
Fragments arranged by size:
2522 414 345 248 248 35
BanII G_rGCy'C
Cuts at: 179 187 634 686 826 1856 179
Size: 8 447 52 140 1030 2135
Fragments arranged by size:
2135 1030 447 140 52 8
BbeI G_GCGC'C
Cuts at: 239 1246 1494 239
Size: 1007 248 2557
Fragments arranged by size:
2557 1007 248
BbsI GAAGACnn'nnnn_
Cuts at: 172 696 172
Size: 524 3288
BbvCI CC'TCA_GC
Cuts at: 205 401 662 205
Size: 196 261 3355
Fragments arranged by size:
3355 261 196
BciVI GTATCCnnnnn_n'
Cuts at: 1710 2471 3812 1710
Size: 761 1341 1710
Fragments arranged by size:
1710 1341 761
BclI T'GATC_A
Cuts at: 1332 1332
Size: 3812
BglI GCCn_nnn'nGGC
Cuts at: 151 445 151
Size: 294 3518
BglII A'GATC_T
Cuts at: 1327 3364 1327
Size: 2037 1775
BlpI GC'TnA_GC
Cuts at: 924 924
Size: 3812
Bme1580I G_kGCm'C
Cuts at: 179 1437 1530 2586 179
Size: 1258 93 1056 1405
Fragments arranged by size:
1405 1258 1056 93
BmgBI CAC'GTC
Cuts at: 135 135
Size: 3812
BmrI ACTGGGnnnn_n'
Cuts at: 1024 1105 1440 3584 1024
Size: 81 335 2144 1252
Fragments arranged by size:
2144 1252 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 3812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 397 733 171
Size: 226 336 3250
Fragments arranged by size:
3250 336 226
Bpu10I CC'TnA_GC
Cuts at: 205 401 662 205
Size: 196 261 3355
Fragments arranged by size:
3355 261 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 988 1867 2359 2657 380
Size: 608 879 492 298 1535
Fragments arranged by size:
1535 879 608 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 640 3516 640
Size: 2876 936
BsaAI yAC'GTr
Cuts at: 1165 1165
Size: 3812
BsaBI GATnn'nnATC
Cuts at: 6 1326 3363 6
Size: 1320 2037 455
Fragments arranged by size:
2037 1320 455
BsaHI Gr'CG_yC
Cuts at: 236 1243 1491 2193 3689 236
Size: 1007 248 702 1496 359
Fragments arranged by size:
1496 1007 702 359 248
BsaWI w'CCGG_w
Cuts at: 1001 1522 2200 2474 2621 3032 1001
Size: 521 678 274 147 411 1781
Fragments arranged by size:
1781 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 550 604 163
Size: 387 54 3371
Fragments arranged by size:
3371 387 54
BseYI C'CCAG_C
Cuts at: 1237 1995 2572 3068 1237
Size: 758 577 496 1981
Fragments arranged by size:
1981 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 244
Size: 3812
BsiEI CG_ry'CG
Cuts at: 104 1354 1400 2608 104
Size: 1250 46 1208 1308
Fragments arranged by size:
1308 1250 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 560 826 1604 1794 2586 115
Size: 445 266 778 190 792 1341
Fragments arranged by size:
1341 792 778 445 266 190
BsmBI CGTCTCn'nnnn_
Cuts at: 594 594
Size: 3812
Bsp1286I G_dGCh'C
Cuts at: 115 179 187 560 634 686 826 1437 1530
Size: 64 8 373 74 52 140 611 93
Cuts at: 1530 1604 1794 1856 2586 115
Size: 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 373 190 140 93 74
74 64 62 52 8
BspEI T'CCGG_A
Cuts at: 3032 3032
Size: 3812
BspHI T'CATG_A
Cuts at: 2988 2988
Size: 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 771 862 1076 1378 1759 771
Size: 91 214 302 381 2824
Fragments arranged by size:
2824 381 302 214 91
BsrBI CCG'CTC
Cuts at: 50 2104 2158 3642 3807 50
Size: 2054 54 1484 165 55
Fragments arranged by size:
2054 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 797 1724 797
Size: 927 2885
BsrFI r'CCGG_y
Cuts at: 1810 1991 2200 1810
Size: 181 209 3422
Fragments arranged by size:
3422 209 181
BssSI C'ACGA_G
Cuts at: 2083 2441 2083
Size: 358 3454
BstAPI GCAn_nnn'nTGC
Cuts at: 1189 1189
Size: 3812
BstBI TT'CG_AA
Cuts at: 2173 2173
Size: 3812
BstEII G'GTnAC_C
Cuts at: 721 721
Size: 3812
BstXI CCAn_nnnn'nTGG
Cuts at: 456 693 456
Size: 237 3575
BstYI r'GATC_y
Cuts at: 1 837 1304 1327 1662 1908 2920 3006 3018
Size: 836 467 23 335 246 1012 86 12
Cuts at: 3018 3364 1
Size: 346 449
Fragments arranged by size:
1012 836 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 567 1762 1943 2106 567
Size: 1195 181 163 2273
Fragments arranged by size:
2273 1195 181 163
BtsI GCAGTG_nn'
Cuts at: 1055 1055
Size: 3812
DraI TTT'AAA
Cuts at: 811 811
Size: 3812
DrdI GACnn_nn'nnGTC
Cuts at: 1518 2376 1518
Size: 858 2954
EaeI y'GGCC_r
Cuts at: 143 446 1397 1571 1962 1989 3334 143
Size: 303 951 174 391 27 1345 621
Fragments arranged by size:
1345 951 621 391 303 174 27
EagI C'GGCC_G
Cuts at: 1397 1397
Size: 3812
EarI CTCTTCn'nnn_
Cuts at: 217 253 284 529 1835 2045 217
Size: 36 31 245 1306 210 1984
Fragments arranged by size:
1984 1306 245 210 36 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2020 2330 2476 3622 2020
Size: 310 146 1146 2210
Fragments arranged by size:
2210 1146 310 146
EcoICRI GAG'CTC
Cuts at: 824 824
Size: 3812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 397 733 1637 2069 2816 3084 171
Size: 226 336 904 432 747 268 899
Fragments arranged by size:
904 899 747 432 336 268 226
EcoNI CCTnn'n_nnAGG
Cuts at: 526 639 526
Size: 113 3699
EcoO109I rG'GnC_Cy
Cuts at: 175 176 303 495 519 951 175
Size: 1 127 192 24 432 3036
Fragments arranged by size:
3036 432 192 127 24 1
EcoRI G'AATT_C
Cuts at: 816 816
Size: 3812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1831 1831
Size: 3812
FspI TGC'GCA
Cuts at: 1593 1593
Size: 3812
HaeII r_GCGC'y
Cuts at: 157 239 737 1246 1494 2516 3495 157
Size: 82 498 509 248 1022 979 474
Fragments arranged by size:
1022 979 509 498 474 248 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1654 1684 1686 1716 1654
Size: 30 2 30 3750
Fragments arranged by size:
3750 30 30 2
HincII GTy'rAC
Cuts at: 609 851 609
Size: 242 3570
KasI G'GCGC_C
Cuts at: 235 1242 1490 235
Size: 1007 248 2557
Fragments arranged by size:
2557 1007 248
KpnI G_GTAC'C
Cuts at: 832 832
Size: 3812
MluI A'CGCG_T
Cuts at: 3245 3245
Size: 3812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2483 2667 2483
Size: 184 3628
MscI TGG'CCA
Cuts at: 448 1573 448
Size: 1125 2687
MslI CAynn'nnrTG
Cuts at: 553 1801 1928 553
Size: 1248 127 2437
Fragments arranged by size:
2437 1248 127
MspA1I CmG'CkG
Cuts at: 477 711 923 1237 1597 2610 2855 477
Size: 234 212 314 360 1013 245 1434
Fragments arranged by size:
1434 1013 360 314 245 234 212
NaeI GCC'GGC
Cuts at: 1993 1993
Size: 3812
NarI GG'CG_CC
Cuts at: 236 1243 1491 236
Size: 1007 248 2557
Fragments arranged by size:
2557 1007 248
NgoMIV G'CCGG_C
Cuts at: 1991 1991
Size: 3812
NspI r_CATG'y
Cuts at: 865 1365 2272 865
Size: 500 907 2405
Fragments arranged by size:
2405 907 500
PasI CC'CwG_GG
Cuts at: 180 299 180
Size: 119 3693
PciI A'CATG_T
Cuts at: 2268 2268
Size: 3812
PflMI CCAn_nnn'nTGG
Cuts at: 480 674 480
Size: 194 3618
PfoI T'CCnGG_A
Cuts at: 128 128
Size: 3812
PmeI GTTT'AAAC
Cuts at: 811 811
Size: 3812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2983 2951
Size: 32 3780
PpuMI rG'GwC_Cy
Cuts at: 303 519 303
Size: 216 3596
PshAI GACnn'nnGTC
Cuts at: 604 604
Size: 3812
PspOMI G'GGCC_C
Cuts at: 175 175
Size: 3812
PstI C_TGCA'G
Cuts at: 859 1544 859
Size: 685 3127
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3812
PvuII CAG'CTG
Cuts at: 477 711 1237 1597 477
Size: 234 526 360 2692
Fragments arranged by size:
2692 526 360 234
RsrII CG'GwC_CG
Cuts at: 2007 2007
Size: 3812
SacI G_AGCT'C
Cuts at: 826 826
Size: 3812
SalI G'TCGA_C
Cuts at: 849 849
Size: 3812
SapI GCTCTTCn'nnn_
Cuts at: 253 1835 2045 253
Size: 1582 210 2020
Fragments arranged by size:
2020 1582 210
SbfI CC_TGCA'GG
Cuts at: 859 859
Size: 3812
ScaI AGT'ACT
Cuts at: 3329 3329
Size: 3812
SfcI C'TryA_G
Cuts at: 33 371 855 1540 2533 2724 33
Size: 338 484 685 993 191 1121
Fragments arranged by size:
1121 993 685 484 338 191
SfoI GGC'GCC
Cuts at: 237 1244 1492 237
Size: 1007 248 2557
Fragments arranged by size:
2557 1007 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
SmaI CCC'GGG
Cuts at: 834 834
Size: 3812
SmlI C'TyrA_G
Cuts at: 395 967 1882 2374 2636 395
Size: 572 915 492 262 1571
Fragments arranged by size:
1571 915 572 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3812
SphI G_CATG'C
Cuts at: 865 1365 865
Size: 500 3312
StyI C'CwwG_G
Cuts at: 534 602 946 1300 534
Size: 68 344 354 3046
Fragments arranged by size:
3046 354 344 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 565 580 740 1395 1528 1987 2196 565
Size: 15 160 655 133 459 209 2181
Fragments arranged by size:
2181 655 459 209 160 133 15
TatI w'GTAC_w
Cuts at: 3327 3327
Size: 3812
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 3780
Tth111I GACn'n_nGTC
Cuts at: 1609 1609
Size: 3812
XbaI T'CTAG_A
Cuts at: 843 843
Size: 3812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 441 1307 441
Size: 866 2946
XmaI C'CCGG_G
Cuts at: 832 832
Size: 3812
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsaXI BsiWI BsmI BsrGI
BssHII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PmlI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI