Restriction map for
pF1KB7573
(Circular) (Six-base) MAPSORT of: pF1KB7573.seq Check: 1612 from: 1 to: 3749
pF1KB7573 3749 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 711 711
Size: 3749
AccI GT'mk_AC
Cuts at: 656 787 656
Size: 131 3618
Acc65I G'GTAC_C
Cuts at: 455 765 455
Size: 310 3439
AclI AA'CG_TT
Cuts at: 3589 3589
Size: 3749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1574 2006 2753 3021 1574
Size: 432 747 268 2302
Fragments arranged by size:
2302 747 432 268
AflIII A'CryG_T
Cuts at: 1101 2205 3182 1101
Size: 1104 977 1668
Fragments arranged by size:
1668 1104 977
AgeI A'CCGG_T
Cuts at: 2137 2137
Size: 3749
AlwNI CAG_nnn'CTG
Cuts at: 1054 2621 1054
Size: 1567 2182
ApaI G_GGCC'C
Cuts at: 506 506
Size: 3749
ApaLI G'TGCA_C
Cuts at: 2519 2519
Size: 3749
ApoI r'AATT_y
Cuts at: 753 3102 3358 753
Size: 2349 256 1144
Fragments arranged by size:
2349 1144 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3749
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
AvaI C'yCGr_G
Cuts at: 443 512 580 769 443
Size: 69 68 189 3423
Fragments arranged by size:
3423 189 69 68
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 662 695 662
Size: 33 3716
BamHI G'GATC_C
Cuts at: 774 774
Size: 3749
BanI G'GyrC_C
Cuts at: 448 455 723 765 1179 1427 1462 448
Size: 7 268 42 414 248 35 2735
Fragments arranged by size:
2735 414 268 248 42 35 7
BanII G_rGCy'C
Cuts at: 176 506 624 763 1793 176
Size: 330 118 139 1030 2132
Fragments arranged by size:
2132 1030 330 139 118
BbeI G_GCGC'C
Cuts at: 1183 1431 1183
Size: 248 3501
BciVI GTATCCnnnnn_n'
Cuts at: 1647 2408 3749 1647
Size: 761 1341 1647
Fragments arranged by size:
1647 1341 761
BclI T'GATC_A
Cuts at: 1269 1269
Size: 3749
BglII A'GATC_T
Cuts at: 1264 3301 1264
Size: 2037 1712
BlpI GC'TnA_GC
Cuts at: 861 861
Size: 3749
Bme1580I G_kGCm'C
Cuts at: 451 490 506 513 1374 1467 2523 451
Size: 39 16 7 861 93 1056 1677
Fragments arranged by size:
1677 1056 861 93 39 16 7
BmrI ACTGGGnnnn_n'
Cuts at: 500 961 1042 1377 3521 500
Size: 461 81 335 2144 728
Fragments arranged by size:
2144 728 461 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 3749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 588 588
Size: 3749
Bpu10I CC'TnA_GC
Cuts at: 422 422
Size: 3749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 925 1804 2296 2594 385
Size: 540 879 492 298 1540
Fragments arranged by size:
1540 879 540 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 433 3453 433
Size: 3020 729
BsaAI yAC'GTr
Cuts at: 1102 1102
Size: 3749
BsaBI GATnn'nnATC
Cuts at: 6 1263 3300 6
Size: 1257 2037 455
Fragments arranged by size:
2037 1257 455
BsaHI Gr'CG_yC
Cuts at: 1180 1428 2130 3626 1180
Size: 248 702 1496 1303
Fragments arranged by size:
1496 1303 702 248
BsaWI w'CCGG_w
Cuts at: 938 1459 2137 2411 2558 2969 938
Size: 521 678 274 147 411 1718
Fragments arranged by size:
1718 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 520 550 520
Size: 30 3719
BseYI C'CCAG_C
Cuts at: 663 733 1174 1932 2509 3005 663
Size: 70 441 758 577 496 1407
Fragments arranged by size:
1407 758 577 496 441 70
BsiEI CG_ry'CG
Cuts at: 104 254 1291 1337 2545 104
Size: 150 1037 46 1208 1308
Fragments arranged by size:
1308 1208 1037 150 46
BsiHKAI G_wGCw'C
Cuts at: 176 763 1541 1731 2523 176
Size: 587 778 190 792 1402
Fragments arranged by size:
1402 792 778 587 190
Bsp1286I G_dGCh'C
Cuts at: 176 451 490 506 513 624 763 1374 1467
Size: 275 39 16 7 111 139 611 93
Cuts at: 1467 1541 1731 1793 2523 176
Size: 74 190 62 730 1402
Fragments arranged by size:
1402 730 611 275 190 139 111 93
74 62 39 16 7
BspEI T'CCGG_A
Cuts at: 2969 2969
Size: 3749
BspHI T'CATG_A
Cuts at: 2925 2925
Size: 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 711 799 1013 1315 1696 711
Size: 88 214 302 381 2764
Fragments arranged by size:
2764 381 302 214 88
BsrBI CCG'CTC
Cuts at: 50 2041 2095 3579 3744 50
Size: 1991 54 1484 165 55
Fragments arranged by size:
1991 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1661 1661
Size: 3749
BsrFI r'CCGG_y
Cuts at: 726 1747 1928 2137 726
Size: 1021 181 209 2338
Fragments arranged by size:
2338 1021 209 181
BssSI C'ACGA_G
Cuts at: 2020 2378 2020
Size: 358 3391
BstAPI GCAn_nnn'nTGC
Cuts at: 723 1126 723
Size: 403 3346
BstBI TT'CG_AA
Cuts at: 2110 2110
Size: 3749
BstYI r'GATC_y
Cuts at: 1 704 774 1241 1264 1599 1845 2857 2943
Size: 703 70 467 23 335 246 1012 86
Cuts at: 2943 2955 3301 1
Size: 12 346 449
Fragments arranged by size:
1012 703 467 449 346 335 246 86
70 23 12
BtgI C'CryG_G
Cuts at: 107 674 107
Size: 567 3182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1699 1880 2043 1699
Size: 181 163 3405
Fragments arranged by size:
3405 181 163
BtsI GCAGTG_nn'
Cuts at: 635 992 635
Size: 357 3392
DraI TTT'AAA
Cuts at: 748 748
Size: 3749
DrdI GACnn_nn'nnGTC
Cuts at: 123 1455 2313 123
Size: 1332 858 1559
Fragments arranged by size:
1559 1332 858
EaeI y'GGCC_r
Cuts at: 140 251 1334 1508 1899 1926 3271 140
Size: 111 1083 174 391 27 1345 618
Fragments arranged by size:
1345 1083 618 391 174 111 27
EagI C'GGCC_G
Cuts at: 251 1334 251
Size: 1083 2666
EarI CTCTTCn'nnn_
Cuts at: 700 1772 1982 700
Size: 1072 210 2467
Fragments arranged by size:
2467 1072 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1957 2267 2413 3559 1957
Size: 310 146 1146 2147
Fragments arranged by size:
2147 1146 310 146
EcoICRI GAG'CTC
Cuts at: 174 761 174
Size: 587 3162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 588 1574 2006 2753 3021 588
Size: 986 432 747 268 1316
Fragments arranged by size:
1316 986 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 478 478
Size: 3749
EcoO109I rG'GnC_Cy
Cuts at: 502 888 502
Size: 386 3363
EcoRI G'AATT_C
Cuts at: 753 753
Size: 3749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1768 1768
Size: 3749
FspI TGC'GCA
Cuts at: 1530 1530
Size: 3749
HaeII r_GCGC'y
Cuts at: 1183 1431 2453 3432 1183
Size: 248 1022 979 1500
Fragments arranged by size:
1500 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1591 1621 1623 1653 1591
Size: 30 2 30 3687
Fragments arranged by size:
3687 30 30 2
HincII GTy'rAC
Cuts at: 788 788
Size: 3749
KasI G'GCGC_C
Cuts at: 1179 1427 1179
Size: 248 3501
KpnI G_GTAC'C
Cuts at: 459 769 459
Size: 310 3439
MluI A'CGCG_T
Cuts at: 3182 3182
Size: 3749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2420 2604 2420
Size: 184 3565
MscI TGG'CCA
Cuts at: 1510 1510
Size: 3749
MslI CAynn'nnrTG
Cuts at: 112 523 1738 1865 112
Size: 411 1215 127 1996
Fragments arranged by size:
1996 1215 411 127
MspA1I CmG'CkG
Cuts at: 860 1174 1534 2547 2792 860
Size: 314 360 1013 245 1817
Fragments arranged by size:
1817 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1930 1930
Size: 3749
NarI GG'CG_CC
Cuts at: 1180 1428 1180
Size: 248 3501
NcoI C'CATG_G
Cuts at: 107 674 107
Size: 567 3182
NgoMIV G'CCGG_C
Cuts at: 1928 1928
Size: 3749
NspI r_CATG'y
Cuts at: 802 1302 2209 802
Size: 500 907 2342
Fragments arranged by size:
2342 907 500
PciI A'CATG_T
Cuts at: 2205 2205
Size: 3749
PflMI CCAn_nnn'nTGG
Cuts at: 687 687
Size: 3749
PmeI GTTT'AAAC
Cuts at: 748 748
Size: 3749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2888 2920 2888
Size: 32 3717
PspOMI G'GGCC_C
Cuts at: 502 502
Size: 3749
PspXI vC'TCGA_Gb
Cuts at: 580 580
Size: 3749
PstI C_TGCA'G
Cuts at: 796 1481 796
Size: 685 3064
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3749
PvuII CAG'CTG
Cuts at: 1174 1534 1174
Size: 360 3389
RsrII CG'GwC_CG
Cuts at: 1944 1944
Size: 3749
SacI G_AGCT'C
Cuts at: 176 763 176
Size: 587 3162
SalI G'TCGA_C
Cuts at: 786 786
Size: 3749
SapI GCTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
SbfI CC_TGCA'GG
Cuts at: 796 796
Size: 3749
ScaI AGT'ACT
Cuts at: 463 3266 463
Size: 2803 946
SexAI A'CCwGG_T
Cuts at: 451 451
Size: 3749
SfcI C'TryA_G
Cuts at: 33 589 792 1477 2470 2661 33
Size: 556 203 685 993 191 1121
Fragments arranged by size:
1121 993 685 556 203 191
SfoI GGC'GCC
Cuts at: 1181 1429 1181
Size: 248 3501
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
SmaI CCC'GGG
Cuts at: 445 514 771 445
Size: 69 257 3423
Fragments arranged by size:
3423 257 69
SmlI C'TyrA_G
Cuts at: 400 580 904 1819 2311 2573 400
Size: 180 324 915 492 262 1576
Fragments arranged by size:
1576 915 492 324 262 180
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3749
SphI G_CATG'C
Cuts at: 802 1302 802
Size: 500 3249
StyI C'CwwG_G
Cuts at: 107 264 273 674 883 1237 107
Size: 157 9 401 209 354 2619
Fragments arranged by size:
2619 401 354 209 157 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1332 1465 1924 2133 1332
Size: 133 459 209 2948
Fragments arranged by size:
2948 459 209 133
TatI w'GTAC_w
Cuts at: 461 683 3264 461
Size: 222 2581 946
Fragments arranged by size:
2581 946 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 3717
Tth111I GACn'n_nGTC
Cuts at: 1546 1546
Size: 3749
XbaI T'CTAG_A
Cuts at: 780 780
Size: 3749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1244 1244
Size: 3749
XhoI C'TCGA_G
Cuts at: 580 580
Size: 3749
XmaI C'CCGG_G
Cuts at: 443 512 769 443
Size: 69 257 3423
Fragments arranged by size:
3423 257 69
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI