Restriction map for
pF1KB7562
(Circular) (Six-base) MAPSORT of: pF1KB7562.seq Check: 4247 from: 1 to: 3935
pF1KB7562 3935 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 973 973
Size: 3935
Acc65I G'GTAC_C
Cuts at: 951 951
Size: 3935
AclI AA'CG_TT
Cuts at: 3775 3775
Size: 3935
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1760 2192 2939 3207 193
Size: 1567 432 747 268 921
Fragments arranged by size:
1567 921 747 432 268
AflIII A'CryG_T
Cuts at: 1287 2391 3368 1287
Size: 1104 977 1854
Fragments arranged by size:
1854 1104 977
AgeI A'CCGG_T
Cuts at: 2323 2323
Size: 3935
AleI CACnn'nnGTG
Cuts at: 116 116
Size: 3935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 148 848 148
Size: 700 3235
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 593 625 593
Size: 32 3903
AlwNI CAG_nnn'CTG
Cuts at: 303 1240 2807 303
Size: 937 1567 1431
Fragments arranged by size:
1567 1431 937
ApaI G_GGCC'C
Cuts at: 433 433
Size: 3935
ApaLI G'TGCA_C
Cuts at: 2705 2705
Size: 3935
ApoI r'AATT_y
Cuts at: 939 3288 3544 939
Size: 2349 256 1330
Fragments arranged by size:
2349 1330 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3935
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
AvaI C'yCGr_G
Cuts at: 486 733 955 486
Size: 247 222 3466
Fragments arranged by size:
3466 247 222
BamHI G'GATC_C
Cuts at: 870 960 870
Size: 90 3845
BanI G'GyrC_C
Cuts at: 951 1365 1613 1648 951
Size: 414 248 35 3238
Fragments arranged by size:
3238 414 248 35
BanII G_rGCy'C
Cuts at: 395 425 433 949 1979 395
Size: 30 8 516 1030 2351
Fragments arranged by size:
2351 1030 516 30 8
BbeI G_GCGC'C
Cuts at: 1369 1617 1369
Size: 248 3687
BbsI GAAGACnn'nnnn_
Cuts at: 662 662
Size: 3935
BbvCI CC'TCA_GC
Cuts at: 416 416
Size: 3935
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3901
BciVI GTATCCnnnnn_n'
Cuts at: 1833 2594 3935 1833
Size: 761 1341 1833
Fragments arranged by size:
1833 1341 761
BclI T'GATC_A
Cuts at: 1455 1455
Size: 3935
BglI GCCn_nnn'nGGC
Cuts at: 316 316
Size: 3935
BglII A'GATC_T
Cuts at: 1450 3487 1450
Size: 2037 1898
BlpI GC'TnA_GC
Cuts at: 381 506 1047 381
Size: 125 541 3269
Fragments arranged by size:
3269 541 125
Bme1580I G_kGCm'C
Cuts at: 361 433 562 690 1560 1653 2709 361
Size: 72 129 128 870 93 1056 1587
Fragments arranged by size:
1587 1056 870 129 128 93 72
BmgBI CAC'GTC
Cuts at: 808 808
Size: 3935
BmrI ACTGGGnnnn_n'
Cuts at: 1147 1228 1563 3707 1147
Size: 81 335 2144 1375
Fragments arranged by size:
2144 1375 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 3935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 490 503 538 676 760 371
Size: 119 13 35 138 84 3546
Fragments arranged by size:
3546 138 119 84 35 13
Bpu10I CC'TnA_GC
Cuts at: 416 416
Size: 3935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 1111 1990 2482 2780 902
Size: 209 879 492 298 2057
Fragments arranged by size:
2057 879 492 298 209
BsaI GGTCTCn'nnnn_
Cuts at: 3639 3639
Size: 3935
BsaAI yAC'GTr
Cuts at: 342 1288 342
Size: 946 2989
BsaBI GATnn'nnATC
Cuts at: 6 1449 3486 6
Size: 1443 2037 455
Fragments arranged by size:
2037 1443 455
BsaHI Gr'CG_yC
Cuts at: 236 1366 1614 2316 3812 236
Size: 1130 248 702 1496 359
Fragments arranged by size:
1496 1130 702 359 248
BsaWI w'CCGG_w
Cuts at: 1124 1645 2323 2597 2744 3155 1124
Size: 521 678 274 147 411 1904
Fragments arranged by size:
1904 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 283 313 705 735 283
Size: 30 392 30 3483
Fragments arranged by size:
3483 392 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 541 900 541
Size: 359 3576
BseYI C'CCAG_C
Cuts at: 200 404 501 544 622 697 762 874 1360
Size: 204 97 43 78 75 65 112 486
Cuts at: 1360 2118 2695 3191 200
Size: 758 577 496 944
Fragments arranged by size:
944 758 577 496 486 204 112 97
78 75 65 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 322 226
Size: 96 3839
BsiEI CG_ry'CG
Cuts at: 104 1477 1523 2731 104
Size: 1373 46 1208 1308
Fragments arranged by size:
1373 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 827 949 1727 1917 2709 827
Size: 122 778 190 792 2053
Fragments arranged by size:
2053 792 778 190 122
Bsp1286I G_dGCh'C
Cuts at: 361 395 425 433 562 690 827 949 1560
Size: 34 30 8 129 128 137 122 611
Cuts at: 1560 1653 1727 1917 1979 2709 361
Size: 93 74 190 62 730 1587
Fragments arranged by size:
1587 730 611 190 137 129 128 122
93 74 62 34 30 8
BspEI T'CCGG_A
Cuts at: 3155 3155
Size: 3935
BspHI T'CATG_A
Cuts at: 534 3111 534
Size: 2577 1358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 840 985 1199 1501 1882 840
Size: 145 214 302 381 2893
Fragments arranged by size:
2893 381 302 214 145
BsrBI CCG'CTC
Cuts at: 50 513 2227 2281 3765 3930 50
Size: 463 1714 54 1484 165 55
Fragments arranged by size:
1714 1484 463 165 55 54
BsrDI GCAATG_nn'
Cuts at: 132 1847 132
Size: 1715 2220
BsrFI r'CCGG_y
Cuts at: 209 1933 2114 2323 209
Size: 1724 181 209 1821
Fragments arranged by size:
1821 1724 209 181
BssSI C'ACGA_G
Cuts at: 253 2206 2564 253
Size: 1953 358 1624
Fragments arranged by size:
1953 1624 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1312 1312
Size: 3935
BstBI TT'CG_AA
Cuts at: 2296 2296
Size: 3935
BstYI r'GATC_y
Cuts at: 1 293 870 960 1427 1450 1785 2031 3043
Size: 292 577 90 467 23 335 246 1012
Cuts at: 3043 3129 3141 3487 1
Size: 86 12 346 449
Fragments arranged by size:
1012 577 467 449 346 335 292 246
90 86 23 12
BtgI C'CryG_G
Cuts at: 362 362
Size: 3935
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1885 2066 2229 1885
Size: 181 163 3591
Fragments arranged by size:
3591 181 163
BtsI GCAGTG_nn'
Cuts at: 1178 1178
Size: 3935
DraI TTT'AAA
Cuts at: 934 934
Size: 3935
DrdI GACnn_nn'nnGTC
Cuts at: 1641 2499 1641
Size: 858 3077
EaeI y'GGCC_r
Cuts at: 1520 1694 2085 2112 3457 1520
Size: 174 391 27 1345 1998
Fragments arranged by size:
1998 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1520 1520
Size: 3935
EarI CTCTTCn'nnn_
Cuts at: 1958 2168 1958
Size: 210 3725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2143 2453 2599 3745 2143
Size: 310 146 1146 2333
Fragments arranged by size:
2333 1146 310 146
EcoICRI GAG'CTC
Cuts at: 947 947
Size: 3935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 371 490 503 538 676 760 1760 2192
Size: 178 119 13 35 138 84 1000 432
Cuts at: 2192 2939 3207 193
Size: 747 268 921
Fragments arranged by size:
1000 921 747 432 268 178 138 119
84 35 13
EcoNI CCTnn'n_nnAGG
Cuts at: 286 286
Size: 3935
EcoO109I rG'GnC_Cy
Cuts at: 430 767 1074 430
Size: 337 307 3291
Fragments arranged by size:
3291 337 307
EcoRI G'AATT_C
Cuts at: 939 939
Size: 3935
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1954 1954
Size: 3935
FspI TGC'GCA
Cuts at: 1716 1716
Size: 3935
HaeII r_GCGC'y
Cuts at: 354 1369 1617 2639 3618 354
Size: 1015 248 1022 979 671
Fragments arranged by size:
1022 1015 979 671 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 283 315 358 390 1777 1807 1809 1839 283
Size: 32 43 32 1387 30 2 30 2379
Fragments arranged by size:
2379 1387 43 32 32 30 30 2
HincII GTy'rAC
Cuts at: 974 974
Size: 3935
KasI G'GCGC_C
Cuts at: 1365 1613 1365
Size: 248 3687
KpnI G_GTAC'C
Cuts at: 955 955
Size: 3935
MluI A'CGCG_T
Cuts at: 3368 3368
Size: 3935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2606 2790 2606
Size: 184 3751
MscI TGG'CCA
Cuts at: 1696 1696
Size: 3935
MslI CAynn'nnrTG
Cuts at: 116 344 1924 2051 116
Size: 228 1580 127 2000
Fragments arranged by size:
2000 1580 228 127
MspA1I CmG'CkG
Cuts at: 171 468 589 615 1046 1360 1720 2733 2978
Size: 297 121 26 431 314 360 1013 245
Cuts at: 2978 171
Size: 1128
Fragments arranged by size:
1128 1013 431 360 314 297 245 121
26
NaeI GCC'GGC
Cuts at: 211 2116 211
Size: 1905 2030
NarI GG'CG_CC
Cuts at: 1366 1614 1366
Size: 248 3687
NcoI C'CATG_G
Cuts at: 362 362
Size: 3935
NgoMIV G'CCGG_C
Cuts at: 209 2114 209
Size: 1905 2030
NspI r_CATG'y
Cuts at: 988 1488 2395 988
Size: 500 907 2528
Fragments arranged by size:
2528 907 500
PasI CC'CwG_GG
Cuts at: 795 795
Size: 3935
PciI A'CATG_T
Cuts at: 2391 2391
Size: 3935
PmeI GTTT'AAAC
Cuts at: 934 934
Size: 3935
PmlI CAC'GTG
Cuts at: 342 342
Size: 3935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 3903
PpuMI rG'GwC_Cy
Cuts at: 767 767
Size: 3935
PspOMI G'GGCC_C
Cuts at: 429 429
Size: 3935
PspXI vC'TCGA_Gb
Cuts at: 733 733
Size: 3935
PstI C_TGCA'G
Cuts at: 308 665 815 982 1667 308
Size: 357 150 167 685 2576
Fragments arranged by size:
2576 685 357 167 150
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3935
PvuII CAG'CTG
Cuts at: 171 468 615 1360 1720 171
Size: 297 147 745 360 2386
Fragments arranged by size:
2386 745 360 297 147
RsrII CG'GwC_CG
Cuts at: 2130 2130
Size: 3935
SacI G_AGCT'C
Cuts at: 949 949
Size: 3935
SalI G'TCGA_C
Cuts at: 972 972
Size: 3935
SapI GCTCTTCn'nnn_
Cuts at: 1958 2168 1958
Size: 210 3725
SbfI CC_TGCA'GG
Cuts at: 308 665 982 308
Size: 357 317 3261
Fragments arranged by size:
3261 357 317
ScaI AGT'ACT
Cuts at: 3452 3452
Size: 3935
SfcI C'TryA_G
Cuts at: 33 304 661 811 978 1663 2656 2847 33
Size: 271 357 150 167 685 993 191 1121
Fragments arranged by size:
1121 993 685 357 271 191 167 150
SfoI GGC'GCC
Cuts at: 1367 1615 1367
Size: 248 3687
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
SmaI CCC'GGG
Cuts at: 488 957 488
Size: 469 3466
SmlI C'TyrA_G
Cuts at: 733 917 1090 2005 2497 2759 733
Size: 184 173 915 492 262 1909
Fragments arranged by size:
1909 915 492 262 184 173
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3935
SphI G_CATG'C
Cuts at: 988 1488 988
Size: 500 3435
StyI C'CwwG_G
Cuts at: 152 362 579 1069 1423 152
Size: 210 217 490 354 2664
Fragments arranged by size:
2664 490 354 217 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1518 1651 2110 2319 1518
Size: 133 459 209 3134
Fragments arranged by size:
3134 459 209 133
TatI w'GTAC_w
Cuts at: 3450 3450
Size: 3935
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 637 669 3452 3484 637
Size: 32 2783 32 1088
Fragments arranged by size:
2783 1088 32 32
Tth111I GACn'n_nGTC
Cuts at: 1732 1732
Size: 3935
XbaI T'CTAG_A
Cuts at: 966 966
Size: 3935
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 759 1430 759
Size: 671 3264
XhoI C'TCGA_G
Cuts at: 733 733
Size: 3935
XmaI C'CCGG_G
Cuts at: 486 955 486
Size: 469 3466
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PshAI PsiI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI