Restriction map for
pF1KB7557
(Circular) (Six-base) MAPSORT of: pF1KB7557.seq Check: 3906 from: 1 to: 3719
pF1KB7557 3719 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 667 757 667
Size: 90 3629
Acc65I G'GTAC_C
Cuts at: 735 735
Size: 3719
AclI AA'CG_TT
Cuts at: 253 3559 253
Size: 3306 413
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 521 1544 1976 2723 2991 199
Size: 322 1023 432 747 268 927
Fragments arranged by size:
1023 927 747 432 322 268
AflIII A'CryG_T
Cuts at: 1071 2175 3152 1071
Size: 1104 977 1638
Fragments arranged by size:
1638 1104 977
AgeI A'CCGG_T
Cuts at: 2107 2107
Size: 3719
AleI CACnn'nnGTG
Cuts at: 157 157
Size: 3719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 530 530
Size: 3719
AlwNI CAG_nnn'CTG
Cuts at: 1024 2591 1024
Size: 1567 2152
ApaI G_GGCC'C
Cuts at: 595 595
Size: 3719
ApaLI G'TGCA_C
Cuts at: 2489 2489
Size: 3719
ApoI r'AATT_y
Cuts at: 454 723 3072 3328 454
Size: 269 2349 256 845
Fragments arranged by size:
2349 845 269 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
AvaI C'yCGr_G
Cuts at: 739 739
Size: 3719
BamHI G'GATC_C
Cuts at: 744 744
Size: 3719
BanI G'GyrC_C
Cuts at: 151 735 1149 1397 1432 151
Size: 584 414 248 35 2438
Fragments arranged by size:
2438 584 414 248 35
BanII G_rGCy'C
Cuts at: 595 733 1763 595
Size: 138 1030 2551
Fragments arranged by size:
2551 1030 138
BbeI G_GCGC'C
Cuts at: 1153 1401 1153
Size: 248 3471
BbsI GAAGACnn'nnnn_
Cuts at: 400 400
Size: 3719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 237 271 237
Size: 34 3685
BciVI GTATCCnnnnn_n'
Cuts at: 1617 2378 3719 1617
Size: 761 1341 1617
Fragments arranged by size:
1617 1341 761
BclI T'GATC_A
Cuts at: 1239 1239
Size: 3719
BglII A'GATC_T
Cuts at: 220 1234 3271 220
Size: 1014 2037 668
Fragments arranged by size:
2037 1014 668
BlpI GC'TnA_GC
Cuts at: 831 831
Size: 3719
Bme1580I G_kGCm'C
Cuts at: 595 1344 1437 2493 595
Size: 749 93 1056 1821
Fragments arranged by size:
1821 1056 749 93
BmrI ACTGGGnnnn_n'
Cuts at: 931 1012 1347 3491 931
Size: 81 335 2144 1159
Fragments arranged by size:
2144 1159 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 559 2086 559
Size: 1527 2192
Bpu10I CC'TnA_GC
Cuts at: 167 167
Size: 3719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 549 895 1774 2266 2564 209
Size: 340 346 879 492 298 1364
Fragments arranged by size:
1364 879 492 346 340 298
BsaI GGTCTCn'nnnn_
Cuts at: 3423 3423
Size: 3719
BsaAI yAC'GTr
Cuts at: 1072 1072
Size: 3719
BsaBI GATnn'nnATC
Cuts at: 6 437 1233 3270 6
Size: 431 796 2037 455
Fragments arranged by size:
2037 796 455 431
BsaHI Gr'CG_yC
Cuts at: 1150 1398 2100 3596 1150
Size: 248 702 1496 1273
Fragments arranged by size:
1496 1273 702 248
BsaWI w'CCGG_w
Cuts at: 908 1429 2107 2381 2528 2939 908
Size: 521 678 274 147 411 1688
Fragments arranged by size:
1688 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 574 574
Size: 3719
BseYI C'CCAG_C
Cuts at: 183 588 1144 1902 2479 2975 183
Size: 405 556 758 577 496 927
Fragments arranged by size:
927 758 577 556 496 405
BsiEI CG_ry'CG
Cuts at: 104 1261 1307 2515 104
Size: 1157 46 1208 1308
Fragments arranged by size:
1308 1208 1157 46
BsiHKAI G_wGCw'C
Cuts at: 509 733 1511 1701 2493 509
Size: 224 778 190 792 1735
Fragments arranged by size:
1735 792 778 224 190
BsmI GAATG_Cn'
Cuts at: 659 659
Size: 3719
Bsp1286I G_dGCh'C
Cuts at: 509 595 733 1344 1437 1511 1701 1763 2493
Size: 86 138 611 93 74 190 62 730
Cuts at: 2493 509
Size: 1735
Fragments arranged by size:
1735 730 611 190 138 93 86 74
62
BspEI T'CCGG_A
Cuts at: 2939 2939
Size: 3719
BspHI T'CATG_A
Cuts at: 545 2895 545
Size: 2350 1369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 503 769 983 1285 1666 503
Size: 266 214 302 381 2556
Fragments arranged by size:
2556 381 302 266 214
BsrBI CCG'CTC
Cuts at: 50 2011 2065 3549 3714 50
Size: 1961 54 1484 165 55
Fragments arranged by size:
1961 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1631 1631
Size: 3719
BsrFI r'CCGG_y
Cuts at: 1717 1898 2107 1717
Size: 181 209 3329
Fragments arranged by size:
3329 209 181
BssSI C'ACGA_G
Cuts at: 1990 2348 1990
Size: 358 3361
BstAPI GCAn_nnn'nTGC
Cuts at: 1096 1096
Size: 3719
BstBI TT'CG_AA
Cuts at: 2080 2080
Size: 3719
BstYI r'GATC_y
Cuts at: 1 220 744 1211 1234 1569 1815 2827 2913
Size: 219 524 467 23 335 246 1012 86
Cuts at: 2913 2925 3271 1
Size: 12 346 449
Fragments arranged by size:
1012 524 467 449 346 335 246 219
86 23 12
BtgI C'CryG_G
Cuts at: 107 625 107
Size: 518 3201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1669 1850 2013 1669
Size: 181 163 3375
Fragments arranged by size:
3375 181 163
BtsI GCAGTG_nn'
Cuts at: 962 962
Size: 3719
DraI TTT'AAA
Cuts at: 718 718
Size: 3719
DrdI GACnn_nn'nnGTC
Cuts at: 1425 2283 1425
Size: 858 2861
EaeI y'GGCC_r
Cuts at: 1304 1478 1869 1896 3241 1304
Size: 174 391 27 1345 1782
Fragments arranged by size:
1782 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1304 1304
Size: 3719
EarI CTCTTCn'nnn_
Cuts at: 497 1742 1952 497
Size: 1245 210 2264
Fragments arranged by size:
2264 1245 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1927 2237 2383 3529 1927
Size: 310 146 1146 2117
Fragments arranged by size:
2117 1146 310 146
EcoICRI GAG'CTC
Cuts at: 731 731
Size: 3719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 521 1544 1976 2723 2991 199
Size: 322 1023 432 747 268 927
Fragments arranged by size:
1023 927 747 432 322 268
EcoNI CCTnn'n_nnAGG
Cuts at: 556 556
Size: 3719
EcoO109I rG'GnC_Cy
Cuts at: 343 592 858 343
Size: 249 266 3204
Fragments arranged by size:
3204 266 249
EcoRI G'AATT_C
Cuts at: 723 723
Size: 3719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1738 1738
Size: 3719
FspI TGC'GCA
Cuts at: 518 1500 518
Size: 982 2737
HaeII r_GCGC'y
Cuts at: 1153 1401 2423 3402 1153
Size: 248 1022 979 1470
Fragments arranged by size:
1470 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 185 205 217 237 603 635 1561 1591 1593
Size: 20 12 20 366 32 926 30 2
Cuts at: 1593 1623 185
Size: 30 2281
Fragments arranged by size:
2281 926 366 32 30 30 20 20
12 2
HincII GTy'rAC
Cuts at: 309 668 758 309
Size: 359 90 3270
Fragments arranged by size:
3270 359 90
HindIII A'AGCT_T
Cuts at: 366 366
Size: 3719
KasI G'GCGC_C
Cuts at: 1149 1397 1149
Size: 248 3471
KpnI G_GTAC'C
Cuts at: 739 739
Size: 3719
MluI A'CGCG_T
Cuts at: 3152 3152
Size: 3719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2390 2574 2390
Size: 184 3535
MscI TGG'CCA
Cuts at: 1480 1480
Size: 3719
MslI CAynn'nnrTG
Cuts at: 157 1708 1835 157
Size: 1551 127 2041
Fragments arranged by size:
2041 1551 127
MspA1I CmG'CkG
Cuts at: 588 830 1144 1504 2517 2762 588
Size: 242 314 360 1013 245 1545
Fragments arranged by size:
1545 1013 360 314 245 242
NaeI GCC'GGC
Cuts at: 1900 1900
Size: 3719
NarI GG'CG_CC
Cuts at: 1150 1398 1150
Size: 248 3471
NcoI C'CATG_G
Cuts at: 107 625 107
Size: 518 3201
NgoMIV G'CCGG_C
Cuts at: 1898 1898
Size: 3719
NspI r_CATG'y
Cuts at: 772 1272 2179 772
Size: 500 907 2312
Fragments arranged by size:
2312 907 500
PciI A'CATG_T
Cuts at: 2175 2175
Size: 3719
PflMI CCAn_nnn'nTGG
Cuts at: 244 244
Size: 3719
PmeI GTTT'AAAC
Cuts at: 718 718
Size: 3719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 3687
PpuMI rG'GwC_Cy
Cuts at: 343 343
Size: 3719
PspOMI G'GGCC_C
Cuts at: 591 591
Size: 3719
PstI C_TGCA'G
Cuts at: 766 1451 766
Size: 685 3034
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3719
PvuII CAG'CTG
Cuts at: 588 1144 1504 588
Size: 556 360 2803
Fragments arranged by size:
2803 556 360
RsrII CG'GwC_CG
Cuts at: 1914 1914
Size: 3719
SacI G_AGCT'C
Cuts at: 733 733
Size: 3719
SalI G'TCGA_C
Cuts at: 666 756 666
Size: 90 3629
SanDI GG'GwC_CC
Cuts at: 343 343
Size: 3719
SapI GCTCTTCn'nnn_
Cuts at: 497 1742 1952 497
Size: 1245 210 2264
Fragments arranged by size:
2264 1245 210
SbfI CC_TGCA'GG
Cuts at: 766 766
Size: 3719
ScaI AGT'ACT
Cuts at: 3236 3236
Size: 3719
SfcI C'TryA_G
Cuts at: 33 317 762 1447 2440 2631 33
Size: 284 445 685 993 191 1121
Fragments arranged by size:
1121 993 685 445 284 191
SfoI GGC'GCC
Cuts at: 1151 1399 1151
Size: 248 3471
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
SmaI CCC'GGG
Cuts at: 741 741
Size: 3719
SmlI C'TyrA_G
Cuts at: 224 528 874 1789 2281 2543 224
Size: 304 346 915 492 262 1400
Fragments arranged by size:
1400 915 492 346 304 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3719
SphI G_CATG'C
Cuts at: 772 1272 772
Size: 500 3219
StyI C'CwwG_G
Cuts at: 107 338 625 853 1207 107
Size: 231 287 228 354 2619
Fragments arranged by size:
2619 354 287 231 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1302 1435 1894 2103 1302
Size: 133 459 209 2918
Fragments arranged by size:
2918 459 209 133
TatI w'GTAC_w
Cuts at: 3234 3234
Size: 3719
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 3687
Tth111I GACn'n_nGTC
Cuts at: 1516 1516
Size: 3719
XbaI T'CTAG_A
Cuts at: 474 750 474
Size: 276 3443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1214 1214
Size: 3719
XmaI C'CCGG_G
Cuts at: 739 739
Size: 3719
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI BsaXI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI