Restriction map for
pF1KB7545
(Circular) (Six-base) MAPSORT of: pF1KB7545.seq Check: 5697 from: 1 to: 3665
pF1KB7545 3665 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 438 438
Size: 3665
AccI GT'mk_AC
Cuts at: 155 644 703 155
Size: 489 59 3117
Fragments arranged by size:
3117 489 59
Acc65I G'GTAC_C
Cuts at: 681 681
Size: 3665
AclI AA'CG_TT
Cuts at: 3505 3505
Size: 3665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 1490 1922 2669 2937 557
Size: 933 432 747 268 1285
Fragments arranged by size:
1285 933 747 432 268
AflIII A'CryG_T
Cuts at: 1017 2121 3098 1017
Size: 1104 977 1584
Fragments arranged by size:
1584 1104 977
AgeI A'CCGG_T
Cuts at: 2053 2053
Size: 3665
AlwNI CAG_nnn'CTG
Cuts at: 970 2537 970
Size: 1567 2098
ApaLI G'TGCA_C
Cuts at: 2435 2435
Size: 3665
ApoI r'AATT_y
Cuts at: 669 3018 3274 669
Size: 2349 256 1060
Fragments arranged by size:
2349 1060 256
AseI AT'TA_AT
Cuts at: 20 601 20
Size: 581 3084
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
AvaI C'yCGr_G
Cuts at: 310 400 685 310
Size: 90 285 3290
Fragments arranged by size:
3290 285 90
BamHI G'GATC_C
Cuts at: 690 690
Size: 3665
BanI G'GyrC_C
Cuts at: 681 1095 1343 1378 681
Size: 414 248 35 2968
Fragments arranged by size:
2968 414 248 35
BanII G_rGCy'C
Cuts at: 231 679 1709 231
Size: 448 1030 2187
Fragments arranged by size:
2187 1030 448
BbeI G_GCGC'C
Cuts at: 1099 1347 1099
Size: 248 3417
BbsI GAAGACnn'nnnn_
Cuts at: 545 545
Size: 3665
BbvCI CC'TCA_GC
Cuts at: 182 182
Size: 3665
BciVI GTATCCnnnnn_n'
Cuts at: 1563 2324 3665 1563
Size: 761 1341 1563
Fragments arranged by size:
1563 1341 761
BclI T'GATC_A
Cuts at: 1185 1185
Size: 3665
BglI GCCn_nnn'nGGC
Cuts at: 218 379 218
Size: 161 3504
BglII A'GATC_T
Cuts at: 1180 3217 1180
Size: 2037 1628
BlpI GC'TnA_GC
Cuts at: 777 777
Size: 3665
Bme1580I G_kGCm'C
Cuts at: 500 1290 1383 2439 500
Size: 790 93 1056 1726
Fragments arranged by size:
1726 1056 790 93
BmrI ACTGGGnnnn_n'
Cuts at: 877 958 1293 3437 877
Size: 81 335 2144 1105
Fragments arranged by size:
2144 1105 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 3665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 215
Size: 3665
Bpu10I CC'TnA_GC
Cuts at: 182 182
Size: 3665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1720 2212 2510 841
Size: 879 492 298 1996
Fragments arranged by size:
1996 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3369 3369
Size: 3665
BsaAI yAC'GTr
Cuts at: 622 1018 622
Size: 396 3269
BsaBI GATnn'nnATC
Cuts at: 6 1179 3216 6
Size: 1173 2037 455
Fragments arranged by size:
2037 1173 455
BsaHI Gr'CG_yC
Cuts at: 1096 1344 2046 3542 1096
Size: 248 702 1496 1219
Fragments arranged by size:
1496 1219 702 248
BsaWI w'CCGG_w
Cuts at: 854 1375 2053 2327 2474 2885 854
Size: 521 678 274 147 411 1634
Fragments arranged by size:
1634 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 469 379
Size: 90 3575
BseYI C'CCAG_C
Cuts at: 186 1090 1848 2425 2921 186
Size: 904 758 577 496 930
Fragments arranged by size:
930 904 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1207 1253 2461 104
Size: 1103 46 1208 1308
Fragments arranged by size:
1308 1208 1103 46
BsiHKAI G_wGCw'C
Cuts at: 231 679 1457 1647 2439 231
Size: 448 778 190 792 1457
Fragments arranged by size:
1457 792 778 448 190
Bsp1286I G_dGCh'C
Cuts at: 231 500 679 1290 1383 1457 1647 1709 2439
Size: 269 179 611 93 74 190 62 730
Cuts at: 2439 231
Size: 1457
Fragments arranged by size:
1457 730 611 269 190 179 93 74
62
BspEI T'CCGG_A
Cuts at: 2885 2885
Size: 3665
BspHI T'CATG_A
Cuts at: 2841 2841
Size: 3665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 438 715 929 1231 1612 438
Size: 277 214 302 381 2491
Fragments arranged by size:
2491 381 302 277 214
BsrBI CCG'CTC
Cuts at: 50 1957 2011 3495 3660 50
Size: 1907 54 1484 165 55
Fragments arranged by size:
1907 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1577 1577
Size: 3665
BsrFI r'CCGG_y
Cuts at: 369 1663 1844 2053 369
Size: 1294 181 209 1981
Fragments arranged by size:
1981 1294 209 181
BssSI C'ACGA_G
Cuts at: 274 1936 2294 274
Size: 1662 358 1645
Fragments arranged by size:
1662 1645 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1042 1042
Size: 3665
BstBI TT'CG_AA
Cuts at: 2026 2026
Size: 3665
BstYI r'GATC_y
Cuts at: 1 690 1157 1180 1515 1761 2773 2859 2871
Size: 689 467 23 335 246 1012 86 12
Cuts at: 2871 3217 1
Size: 346 449
Fragments arranged by size:
1012 689 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 156 156
Size: 3665
Bsu36I CC'TnA_GG
Cuts at: 257 257
Size: 3665
BtgI C'CryG_G
Cuts at: 107 383 107
Size: 276 3389
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 308 1615 1796 1959 308
Size: 1307 181 163 2014
Fragments arranged by size:
2014 1307 181 163
BtsI GCAGTG_nn'
Cuts at: 908 908
Size: 3665
DraI TTT'AAA
Cuts at: 664 664
Size: 3665
DrdI GACnn_nn'nnGTC
Cuts at: 640 1371 2229 640
Size: 731 858 2076
Fragments arranged by size:
2076 858 731
EaeI y'GGCC_r
Cuts at: 219 380 1250 1424 1815 1842 3187 219
Size: 161 870 174 391 27 1345 697
Fragments arranged by size:
1345 870 697 391 174 161 27
EagI C'GGCC_G
Cuts at: 1250 1250
Size: 3665
EarI CTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1873 2183 2329 3475 1873
Size: 310 146 1146 2063
Fragments arranged by size:
2063 1146 310 146
EcoICRI GAG'CTC
Cuts at: 229 677 229
Size: 448 3217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 557 1490 1922 2669 2937 215
Size: 342 933 432 747 268 943
Fragments arranged by size:
943 933 747 432 342 268
EcoO109I rG'GnC_Cy
Cuts at: 804 804
Size: 3665
EcoRI G'AATT_C
Cuts at: 669 669
Size: 3665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1684 1684
Size: 3665
FspI TGC'GCA
Cuts at: 1446 1446
Size: 3665
HaeII r_GCGC'y
Cuts at: 1099 1347 2369 3348 1099
Size: 248 1022 979 1416
Fragments arranged by size:
1416 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1507 1537 1539 1569 1507
Size: 30 2 30 3603
Fragments arranged by size:
3603 30 30 2
HincII GTy'rAC
Cuts at: 704 704
Size: 3665
KasI G'GCGC_C
Cuts at: 1095 1343 1095
Size: 248 3417
KpnI G_GTAC'C
Cuts at: 685 685
Size: 3665
MluI A'CGCG_T
Cuts at: 3098 3098
Size: 3665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 498 2336 2520 498
Size: 1838 184 1643
Fragments arranged by size:
1838 1643 184
MscI TGG'CCA
Cuts at: 221 1426 221
Size: 1205 2460
MslI CAynn'nnrTG
Cuts at: 652 1654 1781 652
Size: 1002 127 2536
Fragments arranged by size:
2536 1002 127
MspA1I CmG'CkG
Cuts at: 186 776 1090 1450 2463 2708 186
Size: 590 314 360 1013 245 1143
Fragments arranged by size:
1143 1013 590 360 314 245
NaeI GCC'GGC
Cuts at: 371 1846 371
Size: 1475 2190
NarI GG'CG_CC
Cuts at: 1096 1344 1096
Size: 248 3417
NcoI C'CATG_G
Cuts at: 107 383 107
Size: 276 3389
NgoMIV G'CCGG_C
Cuts at: 369 1844 369
Size: 1475 2190
NspI r_CATG'y
Cuts at: 411 718 1218 2125 411
Size: 307 500 907 1951
Fragments arranged by size:
1951 907 500 307
PciI A'CATG_T
Cuts at: 2121 2121
Size: 3665
PmeI GTTT'AAAC
Cuts at: 664 664
Size: 3665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2804 2836 2804
Size: 32 3633
PstI C_TGCA'G
Cuts at: 712 1397 712
Size: 685 2980
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3665
PvuII CAG'CTG
Cuts at: 186 1090 1450 186
Size: 904 360 2401
Fragments arranged by size:
2401 904 360
RsrII CG'GwC_CG
Cuts at: 1860 1860
Size: 3665
SacI G_AGCT'C
Cuts at: 231 679 231
Size: 448 3217
SalI G'TCGA_C
Cuts at: 702 702
Size: 3665
SapI GCTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
SbfI CC_TGCA'GG
Cuts at: 712 712
Size: 3665
ScaI AGT'ACT
Cuts at: 3182 3182
Size: 3665
SexAI A'CCwGG_T
Cuts at: 158 158
Size: 3665
SfcI C'TryA_G
Cuts at: 33 708 1393 2386 2577 33
Size: 675 685 993 191 1121
Fragments arranged by size:
1121 993 685 675 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 218 379 218
Size: 161 3504
SfoI GGC'GCC
Cuts at: 1097 1345 1097
Size: 248 3417
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
SmaI CCC'GGG
Cuts at: 687 687
Size: 3665
SmlI C'TyrA_G
Cuts at: 820 1735 2227 2489 820
Size: 915 492 262 1996
Fragments arranged by size:
1996 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3665
SphI G_CATG'C
Cuts at: 718 1218 718
Size: 500 3165
StuI AGG'CCT
Cuts at: 236 236
Size: 3665
StyI C'CwwG_G
Cuts at: 107 222 383 799 1153 107
Size: 115 161 416 354 2619
Fragments arranged by size:
2619 416 354 161 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1248 1381 1840 2049 1248
Size: 133 459 209 2864
Fragments arranged by size:
2864 459 209 133
TatI w'GTAC_w
Cuts at: 3180 3180
Size: 3665
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 3633
Tth111I GACn'n_nGTC
Cuts at: 640 1462 640
Size: 822 2843
XbaI T'CTAG_A
Cuts at: 696 696
Size: 3665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 183 1160 183
Size: 977 2688
XmaI C'CCGG_G
Cuts at: 685 685
Size: 3665
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI