Restriction map for
pF1KB7535
(Circular) (Six-base) MAPSORT of: pF1KB7535.seq Check: 5255 from: 1 to: 3629
pF1KB7535 3629 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 667 667
Size: 3629
Acc65I G'GTAC_C
Cuts at: 645 645
Size: 3629
AclI AA'CG_TT
Cuts at: 3469 3469
Size: 3629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 1454 1886 2633 2901 434
Size: 1020 432 747 268 1162
Fragments arranged by size:
1162 1020 747 432 268
AflIII A'CryG_T
Cuts at: 981 2085 3062 981
Size: 1104 977 1548
Fragments arranged by size:
1548 1104 977
AgeI A'CCGG_T
Cuts at: 2017 2017
Size: 3629
AleI CACnn'nnGTG
Cuts at: 205 205
Size: 3629
AlwNI CAG_nnn'CTG
Cuts at: 934 2501 934
Size: 1567 2062
ApaI G_GGCC'C
Cuts at: 416 416
Size: 3629
ApaLI G'TGCA_C
Cuts at: 2399 2399
Size: 3629
ApoI r'AATT_y
Cuts at: 633 2982 3238 633
Size: 2349 256 1024
Fragments arranged by size:
2349 1024 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3629
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
AvaI C'yCGr_G
Cuts at: 649 649
Size: 3629
BamHI G'GATC_C
Cuts at: 654 654
Size: 3629
BanI G'GyrC_C
Cuts at: 645 1059 1307 1342 645
Size: 414 248 35 2932
Fragments arranged by size:
2932 414 248 35
BanII G_rGCy'C
Cuts at: 416 643 1673 416
Size: 227 1030 2372
Fragments arranged by size:
2372 1030 227
BbeI G_GCGC'C
Cuts at: 1063 1311 1063
Size: 248 3381
BciVI GTATCCnnnnn_n'
Cuts at: 1527 2288 3629 1527
Size: 761 1341 1527
Fragments arranged by size:
1527 1341 761
BclI T'GATC_A
Cuts at: 1149 1149
Size: 3629
BglII A'GATC_T
Cuts at: 1144 3181 1144
Size: 2037 1592
BlpI GC'TnA_GC
Cuts at: 741 741
Size: 3629
Bme1580I G_kGCm'C
Cuts at: 416 1254 1347 2403 416
Size: 838 93 1056 1642
Fragments arranged by size:
1642 1056 838 93
BmrI ACTGGGnnnn_n'
Cuts at: 841 922 1257 3401 841
Size: 81 335 2144 1069
Fragments arranged by size:
2144 1069 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3629
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1684 2176 2474 805
Size: 879 492 298 1960
Fragments arranged by size:
1960 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3333 3333
Size: 3629
BsaAI yAC'GTr
Cuts at: 982 982
Size: 3629
BsaBI GATnn'nnATC
Cuts at: 6 1143 3180 6
Size: 1137 2037 455
Fragments arranged by size:
2037 1137 455
BsaHI Gr'CG_yC
Cuts at: 1060 1308 2010 3506 1060
Size: 248 702 1496 1183
Fragments arranged by size:
1496 1183 702 248
BsaWI w'CCGG_w
Cuts at: 818 1339 2017 2291 2438 2849 818
Size: 521 678 274 147 411 1598
Fragments arranged by size:
1598 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 106 136 106
Size: 30 3599
BseYI C'CCAG_C
Cuts at: 1054 1812 2389 2885 1054
Size: 758 577 496 1798
Fragments arranged by size:
1798 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 204 130
Size: 74 3555
BsiEI CG_ry'CG
Cuts at: 104 1171 1217 2425 104
Size: 1067 46 1208 1308
Fragments arranged by size:
1308 1208 1067 46
BsiHKAI G_wGCw'C
Cuts at: 134 643 1421 1611 2403 134
Size: 509 778 190 792 1360
Fragments arranged by size:
1360 792 778 509 190
Bsp1286I G_dGCh'C
Cuts at: 134 416 643 1254 1347 1421 1611 1673 2403
Size: 282 227 611 93 74 190 62 730
Cuts at: 2403 134
Size: 1360
Fragments arranged by size:
1360 730 611 282 227 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2849 2849
Size: 3629
BspHI T'CATG_A
Cuts at: 2805 2805
Size: 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 226 679 893 1195 1576 226
Size: 453 214 302 381 2279
Fragments arranged by size:
2279 453 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1921 1975 3459 3624 50
Size: 1871 54 1484 165 55
Fragments arranged by size:
1871 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1541 1541
Size: 3629
BsrFI r'CCGG_y
Cuts at: 1627 1808 2017 1627
Size: 181 209 3239
Fragments arranged by size:
3239 209 181
BssSI C'ACGA_G
Cuts at: 1900 2258 1900
Size: 358 3271
BstAPI GCAn_nnn'nTGC
Cuts at: 1006 1006
Size: 3629
BstBI TT'CG_AA
Cuts at: 1990 1990
Size: 3629
BstYI r'GATC_y
Cuts at: 1 533 654 1121 1144 1479 1725 2737 2823
Size: 532 121 467 23 335 246 1012 86
Cuts at: 2823 2835 3181 1
Size: 12 346 449
Fragments arranged by size:
1012 532 467 449 346 335 246 121
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1579 1760 1923 1579
Size: 181 163 3285
Fragments arranged by size:
3285 181 163
BtsI GCAGTG_nn'
Cuts at: 872 872
Size: 3629
DraI TTT'AAA
Cuts at: 348 628 348
Size: 280 3349
DrdI GACnn_nn'nnGTC
Cuts at: 1335 2193 1335
Size: 858 2771
EaeI y'GGCC_r
Cuts at: 340 1214 1388 1779 1806 3151 340
Size: 874 174 391 27 1345 818
Fragments arranged by size:
1345 874 818 391 174 27
EagI C'GGCC_G
Cuts at: 1214 1214
Size: 3629
EarI CTCTTCn'nnn_
Cuts at: 202 403 1652 1862 202
Size: 201 1249 210 1969
Fragments arranged by size:
1969 1249 210 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1837 2147 2293 3439 1837
Size: 310 146 1146 2027
Fragments arranged by size:
2027 1146 310 146
EcoICRI GAG'CTC
Cuts at: 641 641
Size: 3629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 1454 1886 2633 2901 434
Size: 1020 432 747 268 1162
Fragments arranged by size:
1162 1020 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 215 554 768 215
Size: 339 214 3076
Fragments arranged by size:
3076 339 214
EcoRI G'AATT_C
Cuts at: 633 633
Size: 3629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 252 1648 252
Size: 1396 2233
FspI TGC'GCA
Cuts at: 1410 1410
Size: 3629
HaeII r_GCGC'y
Cuts at: 1063 1311 2333 3312 1063
Size: 248 1022 979 1380
Fragments arranged by size:
1380 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 287 319 428 457 460 489 1471 1501 1503
Size: 32 109 29 3 29 982 30 2
Cuts at: 1503 1533 287
Size: 30 2383
Fragments arranged by size:
2383 982 109 32 30 30 29 29
3 2
HincII GTy'rAC
Cuts at: 384 668 384
Size: 284 3345
KasI G'GCGC_C
Cuts at: 1059 1307 1059
Size: 248 3381
KpnI G_GTAC'C
Cuts at: 649 649
Size: 3629
MluI A'CGCG_T
Cuts at: 3062 3062
Size: 3629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 372 500 2300 2484 372
Size: 128 1800 184 1517
Fragments arranged by size:
1800 1517 184 128
MscI TGG'CCA
Cuts at: 1390 1390
Size: 3629
MslI CAynn'nnrTG
Cuts at: 205 1618 1745 205
Size: 1413 127 2089
Fragments arranged by size:
2089 1413 127
MspA1I CmG'CkG
Cuts at: 740 1054 1414 2427 2672 740
Size: 314 360 1013 245 1697
Fragments arranged by size:
1697 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1810 1810
Size: 3629
NarI GG'CG_CC
Cuts at: 1060 1308 1060
Size: 248 3381
NgoMIV G'CCGG_C
Cuts at: 1808 1808
Size: 3629
NspI r_CATG'y
Cuts at: 682 1182 2089 682
Size: 500 907 2222
Fragments arranged by size:
2222 907 500
PciI A'CATG_T
Cuts at: 2085 2085
Size: 3629
PmeI GTTT'AAAC
Cuts at: 628 628
Size: 3629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2768 2800 2768
Size: 32 3597
PpuMI rG'GwC_Cy
Cuts at: 215 554 215
Size: 339 3290
PspOMI G'GGCC_C
Cuts at: 412 412
Size: 3629
PstI C_TGCA'G
Cuts at: 676 1361 676
Size: 685 2944
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3629
PvuII CAG'CTG
Cuts at: 1054 1414 1054
Size: 360 3269
RsrII CG'GwC_CG
Cuts at: 1824 1824
Size: 3629
SacI G_AGCT'C
Cuts at: 643 643
Size: 3629
SalI G'TCGA_C
Cuts at: 666 666
Size: 3629
SanDI GG'GwC_CC
Cuts at: 554 554
Size: 3629
SapI GCTCTTCn'nnn_
Cuts at: 202 1652 1862 202
Size: 1450 210 1969
Fragments arranged by size:
1969 1450 210
SbfI CC_TGCA'GG
Cuts at: 676 676
Size: 3629
ScaI AGT'ACT
Cuts at: 3146 3146
Size: 3629
SfcI C'TryA_G
Cuts at: 33 672 1357 2350 2541 33
Size: 639 685 993 191 1121
Fragments arranged by size:
1121 993 685 639 191
SfoI GGC'GCC
Cuts at: 1061 1309 1061
Size: 248 3381
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
SmaI CCC'GGG
Cuts at: 651 651
Size: 3629
SmlI C'TyrA_G
Cuts at: 784 1699 2191 2453 784
Size: 915 492 262 1960
Fragments arranged by size:
1960 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3629
SphI G_CATG'C
Cuts at: 682 1182 682
Size: 500 3129
SspI AAT'ATT
Cuts at: 267 267
Size: 3629
StyI C'CwwG_G
Cuts at: 763 1117 763
Size: 354 3275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1212 1345 1804 2013 1212
Size: 133 459 209 2828
Fragments arranged by size:
2828 459 209 133
TatI w'GTAC_w
Cuts at: 320 3144 320
Size: 2824 805
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3597
Tth111I GACn'n_nGTC
Cuts at: 1426 1426
Size: 3629
XbaI T'CTAG_A
Cuts at: 660 660
Size: 3629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1124 1124
Size: 3629
XmaI C'CCGG_G
Cuts at: 649 649
Size: 3629
XmnI GAAnn'nnTTC
Cuts at: 196 196
Size: 3629
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI