Restriction map for
pF1KB7533
(Circular) (Six-base) MAPSORT of: pF1KB7533.seq Check: 9328 from: 1 to: 3626
pF1KB7533 3626 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 664 664
Size: 3626
Acc65I G'GTAC_C
Cuts at: 642 642
Size: 3626
AclI AA'CG_TT
Cuts at: 3466 3466
Size: 3626
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1451 1883 2630 2898 1451
Size: 432 747 268 2179
Fragments arranged by size:
2179 747 432 268
AflIII A'CryG_T
Cuts at: 511 978 2082 3059 511
Size: 467 1104 977 1078
Fragments arranged by size:
1104 1078 977 467
AgeI A'CCGG_T
Cuts at: 2014 2014
Size: 3626
AlwNI CAG_nnn'CTG
Cuts at: 931 2498 931
Size: 1567 2059
ApaI G_GGCC'C
Cuts at: 152 152
Size: 3626
ApaLI G'TGCA_C
Cuts at: 2396 2396
Size: 3626
ApoI r'AATT_y
Cuts at: 497 630 2979 3235 497
Size: 133 2349 256 888
Fragments arranged by size:
2349 888 256 133
AseI AT'TA_AT
Cuts at: 20 20
Size: 3626
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3626
AvaI C'yCGr_G
Cuts at: 219 646 219
Size: 427 3199
BamHI G'GATC_C
Cuts at: 651 651
Size: 3626
BanI G'GyrC_C
Cuts at: 642 1056 1304 1339 642
Size: 414 248 35 2929
Fragments arranged by size:
2929 414 248 35
BanII G_rGCy'C
Cuts at: 152 640 1670 152
Size: 488 1030 2108
Fragments arranged by size:
2108 1030 488
BbeI G_GCGC'C
Cuts at: 1060 1308 1060
Size: 248 3378
BbsI GAAGACnn'nnnn_
Cuts at: 341 341
Size: 3626
BciVI GTATCCnnnnn_n'
Cuts at: 1524 2285 3626 1524
Size: 761 1341 1524
Fragments arranged by size:
1524 1341 761
BclI T'GATC_A
Cuts at: 1146 1146
Size: 3626
BglI GCCn_nnn'nGGC
Cuts at: 222 222
Size: 3626
BglII A'GATC_T
Cuts at: 1141 3178 1141
Size: 2037 1589
BlpI GC'TnA_GC
Cuts at: 738 738
Size: 3626
Bme1580I G_kGCm'C
Cuts at: 152 1251 1344 2400 152
Size: 1099 93 1056 1378
Fragments arranged by size:
1378 1099 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 304 838 919 1254 3398 304
Size: 534 81 335 2144 532
Fragments arranged by size:
2144 534 532 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1993 1993
Size: 3626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 1681 2173 2471 802
Size: 879 492 298 1957
Fragments arranged by size:
1957 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3330 3330
Size: 3626
BsaAI yAC'GTr
Cuts at: 979 979
Size: 3626
BsaBI GATnn'nnATC
Cuts at: 6 600 1140 3177 6
Size: 594 540 2037 455
Fragments arranged by size:
2037 594 540 455
BsaHI Gr'CG_yC
Cuts at: 1057 1305 2007 3503 1057
Size: 248 702 1496 1180
Fragments arranged by size:
1496 1180 702 248
BsaWI w'CCGG_w
Cuts at: 815 1336 2014 2288 2435 2846 815
Size: 521 678 274 147 411 1595
Fragments arranged by size:
1595 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 520 520
Size: 3626
BseYI C'CCAG_C
Cuts at: 256 1051 1809 2386 2882 256
Size: 795 758 577 496 1000
Fragments arranged by size:
1000 795 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 286 194
Size: 92 3534
BsiEI CG_ry'CG
Cuts at: 104 1168 1214 2422 104
Size: 1064 46 1208 1308
Fragments arranged by size:
1308 1208 1064 46
BsiHKAI G_wGCw'C
Cuts at: 640 1418 1608 2400 640
Size: 778 190 792 1866
Fragments arranged by size:
1866 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 152 640 1251 1344 1418 1608 1670 2400 152
Size: 488 611 93 74 190 62 730 1378
Fragments arranged by size:
1378 730 611 488 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2846 2846
Size: 3626
BspHI T'CATG_A
Cuts at: 2802 2802
Size: 3626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 676 890 1192 1573 676
Size: 214 302 381 2729
Fragments arranged by size:
2729 381 302 214
BsrBI CCG'CTC
Cuts at: 50 610 1918 1972 3456 3621 50
Size: 560 1308 54 1484 165 55
Fragments arranged by size:
1484 1308 560 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1538 1538
Size: 3626
BsrFI r'CCGG_y
Cuts at: 1624 1805 2014 1624
Size: 181 209 3236
Fragments arranged by size:
3236 209 181
BssSI C'ACGA_G
Cuts at: 1897 2255 1897
Size: 358 3268
BstAPI GCAn_nnn'nTGC
Cuts at: 1003 1003
Size: 3626
BstBI TT'CG_AA
Cuts at: 1987 1987
Size: 3626
BstYI r'GATC_y
Cuts at: 1 453 651 1118 1141 1476 1722 2734 2820
Size: 452 198 467 23 335 246 1012 86
Cuts at: 2820 2832 3178 1
Size: 12 346 449
Fragments arranged by size:
1012 467 452 449 346 335 246 198
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 251 251
Size: 3626
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1576 1757 1920 1576
Size: 181 163 3282
Fragments arranged by size:
3282 181 163
BtsI GCAGTG_nn'
Cuts at: 182 869 182
Size: 687 2939
DraI TTT'AAA
Cuts at: 625 625
Size: 3626
DrdI GACnn_nn'nnGTC
Cuts at: 1332 2190 1332
Size: 858 2768
EaeI y'GGCC_r
Cuts at: 425 1211 1385 1776 1803 3148 425
Size: 786 174 391 27 1345 903
Fragments arranged by size:
1345 903 786 391 174 27
EagI C'GGCC_G
Cuts at: 1211 1211
Size: 3626
EarI CTCTTCn'nnn_
Cuts at: 335 1649 1859 335
Size: 1314 210 2102
Fragments arranged by size:
2102 1314 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1834 2144 2290 3436 1834
Size: 310 146 1146 2024
Fragments arranged by size:
2024 1146 310 146
EcoICRI GAG'CTC
Cuts at: 638 638
Size: 3626
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1451 1883 2630 2898 1451
Size: 432 747 268 2179
Fragments arranged by size:
2179 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 149 765 149
Size: 616 3010
EcoRI G'AATT_C
Cuts at: 630 630
Size: 3626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1645 1645
Size: 3626
FspI TGC'GCA
Cuts at: 1407 1407
Size: 3626
HaeII r_GCGC'y
Cuts at: 1060 1308 2330 3309 1060
Size: 248 1022 979 1377
Fragments arranged by size:
1377 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 406 438 1468 1498 1500 1530 406
Size: 32 1030 30 2 30 2502
Fragments arranged by size:
2502 1030 32 30 30 2
HincII GTy'rAC
Cuts at: 509 665 509
Size: 156 3470
KasI G'GCGC_C
Cuts at: 1056 1304 1056
Size: 248 3378
KpnI G_GTAC'C
Cuts at: 646 646
Size: 3626
MluI A'CGCG_T
Cuts at: 3059 3059
Size: 3626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 595 2297 2481 595
Size: 1702 184 1740
Fragments arranged by size:
1740 1702 184
MscI TGG'CCA
Cuts at: 1387 1387
Size: 3626
MslI CAynn'nnrTG
Cuts at: 1615 1742 1615
Size: 127 3499
MspA1I CmG'CkG
Cuts at: 134 737 1051 1411 2424 2669 134
Size: 603 314 360 1013 245 1091
Fragments arranged by size:
1091 1013 603 360 314 245
NaeI GCC'GGC
Cuts at: 1807 1807
Size: 3626
NarI GG'CG_CC
Cuts at: 1057 1305 1057
Size: 248 3378
NgoMIV G'CCGG_C
Cuts at: 1805 1805
Size: 3626
NspI r_CATG'y
Cuts at: 515 679 1179 2086 515
Size: 164 500 907 2055
Fragments arranged by size:
2055 907 500 164
PasI CC'CwG_GG
Cuts at: 590 590
Size: 3626
PciI A'CATG_T
Cuts at: 511 2082 511
Size: 1571 2055
PmeI GTTT'AAAC
Cuts at: 625 625
Size: 3626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2765 2797 2765
Size: 32 3594
PspOMI G'GGCC_C
Cuts at: 148 148
Size: 3626
PstI C_TGCA'G
Cuts at: 673 1358 673
Size: 685 2941
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3626
PvuII CAG'CTG
Cuts at: 134 1051 1411 134
Size: 917 360 2349
Fragments arranged by size:
2349 917 360
RsrII CG'GwC_CG
Cuts at: 1821 1821
Size: 3626
SacI G_AGCT'C
Cuts at: 640 640
Size: 3626
SalI G'TCGA_C
Cuts at: 663 663
Size: 3626
SapI GCTCTTCn'nnn_
Cuts at: 1649 1859 1649
Size: 210 3416
SbfI CC_TGCA'GG
Cuts at: 673 673
Size: 3626
ScaI AGT'ACT
Cuts at: 3143 3143
Size: 3626
SfcI C'TryA_G
Cuts at: 33 669 1354 2347 2538 33
Size: 636 685 993 191 1121
Fragments arranged by size:
1121 993 685 636 191
SfoI GGC'GCC
Cuts at: 1058 1306 1058
Size: 248 3378
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3626
SmaI CCC'GGG
Cuts at: 221 648 221
Size: 427 3199
SmlI C'TyrA_G
Cuts at: 781 1696 2188 2450 781
Size: 915 492 262 1957
Fragments arranged by size:
1957 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3626
SphI G_CATG'C
Cuts at: 679 1179 679
Size: 500 3126
SspI AAT'ATT
Cuts at: 617 617
Size: 3626
StyI C'CwwG_G
Cuts at: 377 760 1114 377
Size: 383 354 2889
Fragments arranged by size:
2889 383 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1209 1342 1801 2010 1209
Size: 133 459 209 2825
Fragments arranged by size:
2825 459 209 133
TatI w'GTAC_w
Cuts at: 3141 3141
Size: 3626
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 3594
Tth111I GACn'n_nGTC
Cuts at: 1423 1423
Size: 3626
XbaI T'CTAG_A
Cuts at: 657 657
Size: 3626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1121 1121
Size: 3626
XmaI C'CCGG_G
Cuts at: 219 646 219
Size: 427 3199
XmnI GAAnn'nnTTC
Cuts at: 121 121
Size: 3626
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI