Restriction map for
pF1KB7526
(Circular) (Six-base) MAPSORT of: pF1KB7526.seq Check: 1274 from: 1 to: 3866
pF1KB7526 3866 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 904 904
Size: 3866
Acc65I G'GTAC_C
Cuts at: 882 882
Size: 3866
AclI AA'CG_TT
Cuts at: 3706 3706
Size: 3866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 514 841 1691 2123 2870 3138 196
Size: 318 327 850 432 747 268 924
Fragments arranged by size:
924 850 747 432 327 318 268
AflIII A'CryG_T
Cuts at: 1218 2322 3299 1218
Size: 1104 977 1785
Fragments arranged by size:
1785 1104 977
AgeI A'CCGG_T
Cuts at: 2254 2254
Size: 3866
AlwNI CAG_nnn'CTG
Cuts at: 174 703 1171 2738 174
Size: 529 468 1567 1302
Fragments arranged by size:
1567 1302 529 468
ApaI G_GGCC'C
Cuts at: 755 755
Size: 3866
ApaLI G'TGCA_C
Cuts at: 2636 2636
Size: 3866
ApoI r'AATT_y
Cuts at: 229 272 870 3219 3475 229
Size: 43 598 2349 256 620
Fragments arranged by size:
2349 620 598 256 43
AseI AT'TA_AT
Cuts at: 20 734 20
Size: 714 3152
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3866
AvaI C'yCGr_G
Cuts at: 886 886
Size: 3866
BamHI G'GATC_C
Cuts at: 891 891
Size: 3866
BanI G'GyrC_C
Cuts at: 126 882 1296 1544 1579 126
Size: 756 414 248 35 2413
Fragments arranged by size:
2413 756 414 248 35
BanII G_rGCy'C
Cuts at: 755 880 1910 755
Size: 125 1030 2711
Fragments arranged by size:
2711 1030 125
BbeI G_GCGC'C
Cuts at: 130 1300 1548 130
Size: 1170 248 2448
Fragments arranged by size:
2448 1170 248
BciVI GTATCCnnnnn_n'
Cuts at: 1764 2525 3866 1764
Size: 761 1341 1764
Fragments arranged by size:
1764 1341 761
BclI T'GATC_A
Cuts at: 1386 1386
Size: 3866
BglII A'GATC_T
Cuts at: 1381 3418 1381
Size: 2037 1829
BlpI GC'TnA_GC
Cuts at: 978 978
Size: 3866
Bme1580I G_kGCm'C
Cuts at: 755 1491 1584 2640 755
Size: 736 93 1056 1981
Fragments arranged by size:
1981 1056 736 93
BmrI ACTGGGnnnn_n'
Cuts at: 1078 1159 1494 3638 1078
Size: 81 335 2144 1306
Fragments arranged by size:
2144 1306 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 3866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1921 2413 2711 1042
Size: 879 492 298 2197
Fragments arranged by size:
2197 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3570 3570
Size: 3866
BsaAI yAC'GTr
Cuts at: 1219 1219
Size: 3866
BsaBI GATnn'nnATC
Cuts at: 6 1380 3417 6
Size: 1374 2037 455
Fragments arranged by size:
2037 1374 455
BsaHI Gr'CG_yC
Cuts at: 127 1297 1545 2247 3743 127
Size: 1170 248 702 1496 250
Fragments arranged by size:
1496 1170 702 250 248
BsaWI w'CCGG_w
Cuts at: 1055 1576 2254 2528 2675 3086 1055
Size: 521 678 274 147 411 1835
Fragments arranged by size:
1835 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 348 378 348
Size: 30 3836
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 353 353
Size: 3866
BseYI C'CCAG_C
Cuts at: 1291 2049 2626 3122 1291
Size: 758 577 496 2035
Fragments arranged by size:
2035 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 682
Size: 3866
BsiEI CG_ry'CG
Cuts at: 104 1408 1454 2662 104
Size: 1304 46 1208 1308
Fragments arranged by size:
1308 1304 1208 46
BsiHKAI G_wGCw'C
Cuts at: 880 1658 1848 2640 880
Size: 778 190 792 2106
Fragments arranged by size:
2106 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 755 880 1491 1584 1658 1848 1910 2640 755
Size: 125 611 93 74 190 62 730 1981
Fragments arranged by size:
1981 730 611 190 125 93 74 62
BspEI T'CCGG_A
Cuts at: 3086 3086
Size: 3866
BspHI T'CATG_A
Cuts at: 3042 3042
Size: 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 916 1130 1432 1813 916
Size: 214 302 381 2969
Fragments arranged by size:
2969 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2158 2212 3696 3861 50
Size: 2108 54 1484 165 55
Fragments arranged by size:
2108 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1778 1778
Size: 3866
BsrFI r'CCGG_y
Cuts at: 1864 2045 2254 1864
Size: 181 209 3476
Fragments arranged by size:
3476 209 181
BssSI C'ACGA_G
Cuts at: 2137 2495 2137
Size: 358 3508
BstAPI GCAn_nnn'nTGC
Cuts at: 712 1243 712
Size: 531 3335
BstBI TT'CG_AA
Cuts at: 2227 2227
Size: 3866
BstXI CCAn_nnnn'nTGG
Cuts at: 780 780
Size: 3866
BstYI r'GATC_y
Cuts at: 1 170 891 1358 1381 1716 1962 2974 3060
Size: 169 721 467 23 335 246 1012 86
Cuts at: 3060 3072 3418 1
Size: 12 346 449
Fragments arranged by size:
1012 721 467 449 346 335 246 169
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3866
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1816 1997 2160 1816
Size: 181 163 3522
Fragments arranged by size:
3522 181 163
BtsI GCAGTG_nn'
Cuts at: 1109 1109
Size: 3866
DraI TTT'AAA
Cuts at: 865 865
Size: 3866
DrdI GACnn_nn'nnGTC
Cuts at: 1572 2430 1572
Size: 858 3008
EaeI y'GGCC_r
Cuts at: 1451 1625 2016 2043 3388 1451
Size: 174 391 27 1345 1929
Fragments arranged by size:
1929 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1451 1451
Size: 3866
EarI CTCTTCn'nnn_
Cuts at: 817 1889 2099 817
Size: 1072 210 2584
Fragments arranged by size:
2584 1072 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2074 2384 2530 3676 2074
Size: 310 146 1146 2264
Fragments arranged by size:
2264 1146 310 146
EcoICRI GAG'CTC
Cuts at: 878 878
Size: 3866
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 514 841 1691 2123 2870 3138 196
Size: 318 327 850 432 747 268 924
Fragments arranged by size:
924 850 747 432 327 318 268
EcoNI CCTnn'n_nnAGG
Cuts at: 521 521
Size: 3866
EcoO109I rG'GnC_Cy
Cuts at: 442 514 1005 442
Size: 72 491 3303
Fragments arranged by size:
3303 491 72
EcoRI G'AATT_C
Cuts at: 870 870
Size: 3866
EcoRV GAT'ATC
Cuts at: 390 390
Size: 3866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1885 1885
Size: 3866
FspI TGC'GCA
Cuts at: 1647 1647
Size: 3866
HaeII r_GCGC'y
Cuts at: 130 1300 1548 2570 3549 130
Size: 1170 248 1022 979 447
Fragments arranged by size:
1170 1022 979 447 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 114 146 314 346 1708 1738 1740 1770 114
Size: 32 168 32 1362 30 2 30 2210
Fragments arranged by size:
2210 1362 168 32 32 30 30 2
HincII GTy'rAC
Cuts at: 905 905
Size: 3866
KasI G'GCGC_C
Cuts at: 126 1296 1544 126
Size: 1170 248 2448
Fragments arranged by size:
2448 1170 248
KpnI G_GTAC'C
Cuts at: 886 886
Size: 3866
MluI A'CGCG_T
Cuts at: 3299 3299
Size: 3866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 206 2537 2721 206
Size: 2331 184 1351
Fragments arranged by size:
2331 1351 184
MscI TGG'CCA
Cuts at: 1627 1627
Size: 3866
MslI CAynn'nnrTG
Cuts at: 778 836 1855 1982 778
Size: 58 1019 127 2662
Fragments arranged by size:
2662 1019 127 58
MspA1I CmG'CkG
Cuts at: 977 1291 1651 2664 2909 977
Size: 314 360 1013 245 1934
Fragments arranged by size:
1934 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2047 2047
Size: 3866
NarI GG'CG_CC
Cuts at: 127 1297 1545 127
Size: 1170 248 2448
Fragments arranged by size:
2448 1170 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3866
NdeI CA'TA_TG
Cuts at: 435 435
Size: 3866
NgoMIV G'CCGG_C
Cuts at: 2045 2045
Size: 3866
NspI r_CATG'y
Cuts at: 919 1419 2326 919
Size: 500 907 2459
Fragments arranged by size:
2459 907 500
PacI TTA_AT'TAA
Cuts at: 506 506
Size: 3866
PciI A'CATG_T
Cuts at: 2322 2322
Size: 3866
PmeI GTTT'AAAC
Cuts at: 865 865
Size: 3866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 3834
PpuMI rG'GwC_Cy
Cuts at: 442 514 442
Size: 72 3794
PsiI TTA'TAA
Cuts at: 214 214
Size: 3866
PspOMI G'GGCC_C
Cuts at: 751 751
Size: 3866
PstI C_TGCA'G
Cuts at: 717 913 1598 717
Size: 196 685 2985
Fragments arranged by size:
2985 685 196
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3866
PvuII CAG'CTG
Cuts at: 1291 1651 1291
Size: 360 3506
RsrII CG'GwC_CG
Cuts at: 2061 2061
Size: 3866
SacI G_AGCT'C
Cuts at: 880 880
Size: 3866
SalI G'TCGA_C
Cuts at: 903 903
Size: 3866
SapI GCTCTTCn'nnn_
Cuts at: 1889 2099 1889
Size: 210 3656
SbfI CC_TGCA'GG
Cuts at: 913 913
Size: 3866
ScaI AGT'ACT
Cuts at: 3383 3383
Size: 3866
SfcI C'TryA_G
Cuts at: 33 486 584 713 909 1594 2587 2778 33
Size: 453 98 129 196 685 993 191 1121
Fragments arranged by size:
1121 993 685 453 196 191 129 98
SfoI GGC'GCC
Cuts at: 128 1298 1546 128
Size: 1170 248 2448
Fragments arranged by size:
2448 1170 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3866
SmaI CCC'GGG
Cuts at: 888 888
Size: 3866
SmlI C'TyrA_G
Cuts at: 1021 1936 2428 2690 1021
Size: 915 492 262 2197
Fragments arranged by size:
2197 915 492 262
SpeI A'CTAG_T
Cuts at: 67 531 67
Size: 464 3402
SphI G_CATG'C
Cuts at: 919 1419 919
Size: 500 3366
SspI AAT'ATT
Cuts at: 762 762
Size: 3866
StyI C'CwwG_G
Cuts at: 107 155 205 522 1000 1354 107
Size: 48 50 317 478 354 2619
Fragments arranged by size:
2619 478 354 317 50 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1449 1582 2041 2250 1449
Size: 133 459 209 3065
Fragments arranged by size:
3065 459 209 133
TatI w'GTAC_w
Cuts at: 3381 3381
Size: 3866
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3383 3415 3383
Size: 32 3834
Tth111I GACn'n_nGTC
Cuts at: 1663 1663
Size: 3866
XbaI T'CTAG_A
Cuts at: 897 897
Size: 3866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1361 1361
Size: 3866
XmaI C'CCGG_G
Cuts at: 886 886
Size: 3866
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PacI PciI PmeI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PasI PflMI PfoI PmlI PshAI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI