Restriction map for
pF1KB7510
(Circular) (Six-base) MAPSORT of: pF1KB7510.seq Check: 6692 from: 1 to: 3839
pF1KB7510 3839 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 877 877
Size: 3839
Acc65I G'GTAC_C
Cuts at: 385 855 385
Size: 470 3369
AclI AA'CG_TT
Cuts at: 3679 3679
Size: 3839
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 466 1664 2096 2843 3111 132
Size: 334 1198 432 747 268 860
Fragments arranged by size:
1198 860 747 432 334 268
AflIII A'CryG_T
Cuts at: 1191 2295 3272 1191
Size: 1104 977 1758
Fragments arranged by size:
1758 1104 977
AgeI A'CCGG_T
Cuts at: 2227 2227
Size: 3839
AlwNI CAG_nnn'CTG
Cuts at: 234 1144 2711 234
Size: 910 1567 1362
Fragments arranged by size:
1567 1362 910
ApaLI G'TGCA_C
Cuts at: 2609 2609
Size: 3839
ApoI r'AATT_y
Cuts at: 580 843 3192 3448 580
Size: 263 2349 256 971
Fragments arranged by size:
2349 971 263 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3839
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3839
AvaI C'yCGr_G
Cuts at: 859 859
Size: 3839
BamHI G'GATC_C
Cuts at: 864 864
Size: 3839
BanI G'GyrC_C
Cuts at: 181 385 855 1269 1517 1552 181
Size: 204 470 414 248 35 2468
Fragments arranged by size:
2468 470 414 248 204 35
BanII G_rGCy'C
Cuts at: 853 1883 853
Size: 1030 2809
BbeI G_GCGC'C
Cuts at: 185 1273 1521 185
Size: 1088 248 2503
Fragments arranged by size:
2503 1088 248
BbsI GAAGACnn'nnnn_
Cuts at: 149 149
Size: 3839
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 619 653 619
Size: 34 3805
BciVI GTATCCnnnnn_n'
Cuts at: 1737 2498 3839 1737
Size: 761 1341 1737
Fragments arranged by size:
1737 1341 761
BclI T'GATC_A
Cuts at: 1359 1359
Size: 3839
BglII A'GATC_T
Cuts at: 1354 3391 1354
Size: 2037 1802
BlpI GC'TnA_GC
Cuts at: 951 951
Size: 3839
Bme1580I G_kGCm'C
Cuts at: 1464 1557 2613 1464
Size: 93 1056 2690
Fragments arranged by size:
2690 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1051 1132 1467 3611 1051
Size: 81 335 2144 1279
Fragments arranged by size:
2144 1279 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 3839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 491 629 802 244
Size: 247 138 173 3281
Fragments arranged by size:
3281 247 173 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1015 1894 2386 2684 811
Size: 204 879 492 298 1966
Fragments arranged by size:
1966 879 492 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 747 3543 747
Size: 2796 1043
BsaAI yAC'GTr
Cuts at: 1192 1192
Size: 3839
BsaBI GATnn'nnATC
Cuts at: 6 1353 3390 6
Size: 1347 2037 455
Fragments arranged by size:
2037 1347 455
BsaHI Gr'CG_yC
Cuts at: 182 1270 1518 2220 3716 182
Size: 1088 248 702 1496 305
Fragments arranged by size:
1496 1088 702 305 248
BsaWI w'CCGG_w
Cuts at: 1028 1549 2227 2501 2648 3059 1028
Size: 521 678 274 147 411 1808
Fragments arranged by size:
1808 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 403 1264 2022 2599 3095 403
Size: 861 758 577 496 1147
Fragments arranged by size:
1147 861 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1381 1427 2635 104
Size: 1277 46 1208 1308
Fragments arranged by size:
1308 1277 1208 46
BsiHKAI G_wGCw'C
Cuts at: 209 853 1631 1821 2613 209
Size: 644 778 190 792 1435
Fragments arranged by size:
1435 792 778 644 190
BsmI GAATG_Cn'
Cuts at: 702 702
Size: 3839
Bsp1286I G_dGCh'C
Cuts at: 209 853 1464 1557 1631 1821 1883 2613 209
Size: 644 611 93 74 190 62 730 1435
Fragments arranged by size:
1435 730 644 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3059 3059
Size: 3839
BspHI T'CATG_A
Cuts at: 360 3015 360
Size: 2655 1184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 889 1103 1405 1786 889
Size: 214 302 381 2942
Fragments arranged by size:
2942 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2131 2185 3669 3834 50
Size: 2081 54 1484 165 55
Fragments arranged by size:
2081 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 740 1751 740
Size: 1011 2828
BsrFI r'CCGG_y
Cuts at: 196 1837 2018 2227 196
Size: 1641 181 209 1808
Fragments arranged by size:
1808 1641 209 181
BssSI C'ACGA_G
Cuts at: 2110 2468 2110
Size: 358 3481
BstAPI GCAn_nnn'nTGC
Cuts at: 708 1216 708
Size: 508 3331
BstBI TT'CG_AA
Cuts at: 2200 2200
Size: 3839
BstEII G'GTnAC_C
Cuts at: 717 717
Size: 3839
BstXI CCAn_nnnn'nTGG
Cuts at: 650 689 650
Size: 39 3800
BstYI r'GATC_y
Cuts at: 1 864 1331 1354 1689 1935 2947 3033 3045
Size: 863 467 23 335 246 1012 86 12
Cuts at: 3045 3391 1
Size: 346 449
Fragments arranged by size:
1012 863 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 722 107
Size: 615 3224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1789 1970 2133 1789
Size: 181 163 3495
Fragments arranged by size:
3495 181 163
BtsI GCAGTG_nn'
Cuts at: 1082 1082
Size: 3839
DraI TTT'AAA
Cuts at: 838 838
Size: 3839
DrdI GACnn_nn'nnGTC
Cuts at: 1545 2403 1545
Size: 858 2981
EaeI y'GGCC_r
Cuts at: 110 258 1424 1598 1989 2016 3361 110
Size: 148 1166 174 391 27 1345 588
Fragments arranged by size:
1345 1166 588 391 174 148 27
EagI C'GGCC_G
Cuts at: 1424 1424
Size: 3839
EarI CTCTTCn'nnn_
Cuts at: 1862 2072 1862
Size: 210 3629
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 424 2047 2357 2503 3649 424
Size: 1623 310 146 1146 614
Fragments arranged by size:
1623 1146 614 310 146
EcoICRI GAG'CTC
Cuts at: 851 851
Size: 3839
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 244 466 491 629 802 1664 2096 2843
Size: 112 222 25 138 173 862 432 747
Cuts at: 2843 3111 132
Size: 268 860
Fragments arranged by size:
862 860 747 432 268 222 173 138
112 25
EcoNI CCTnn'n_nnAGG
Cuts at: 226 226
Size: 3839
EcoO109I rG'GnC_Cy
Cuts at: 231 978 231
Size: 747 3092
EcoRI G'AATT_C
Cuts at: 843 843
Size: 3839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 291 1858 291
Size: 1567 2272
FspI TGC'GCA
Cuts at: 1620 1620
Size: 3839
HaeII r_GCGC'y
Cuts at: 185 1273 1521 2543 3522 185
Size: 1088 248 1022 979 502
Fragments arranged by size:
1088 1022 979 502 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 328 360 577 609 1681 1711 1713 1743 328
Size: 32 217 32 1072 30 2 30 2424
Fragments arranged by size:
2424 1072 217 32 32 30 30 2
HincII GTy'rAC
Cuts at: 429 878 429
Size: 449 3390
HindIII A'AGCT_T
Cuts at: 133 287 133
Size: 154 3685
KasI G'GCGC_C
Cuts at: 181 1269 1517 181
Size: 1088 248 2503
Fragments arranged by size:
2503 1088 248
KpnI G_GTAC'C
Cuts at: 389 859 389
Size: 470 3369
MluI A'CGCG_T
Cuts at: 3272 3272
Size: 3839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 311 348 2510 2694 311
Size: 37 2162 184 1456
Fragments arranged by size:
2162 1456 184 37
MscI TGG'CCA
Cuts at: 1600 1600
Size: 3839
MslI CAynn'nnrTG
Cuts at: 1828 1955 1828
Size: 127 3712
MspA1I CmG'CkG
Cuts at: 188 950 1264 1624 2637 2882 188
Size: 762 314 360 1013 245 1145
Fragments arranged by size:
1145 1013 762 360 314 245
NaeI GCC'GGC
Cuts at: 2020 2020
Size: 3839
NarI GG'CG_CC
Cuts at: 182 1270 1518 182
Size: 1088 248 2503
Fragments arranged by size:
2503 1088 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3839
NgoMIV G'CCGG_C
Cuts at: 2018 2018
Size: 3839
NspI r_CATG'y
Cuts at: 892 1392 2299 892
Size: 500 907 2432
Fragments arranged by size:
2432 907 500
PciI A'CATG_T
Cuts at: 2295 2295
Size: 3839
PflMI CCAn_nnn'nTGG
Cuts at: 804 804
Size: 3839
PfoI T'CCnGG_A
Cuts at: 221 221
Size: 3839
PmeI GTTT'AAAC
Cuts at: 838 838
Size: 3839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2978 3010 2978
Size: 32 3807
PsiI TTA'TAA
Cuts at: 293 293
Size: 3839
PstI C_TGCA'G
Cuts at: 456 886 1571 456
Size: 430 685 2724
Fragments arranged by size:
2724 685 430
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3839
PvuII CAG'CTG
Cuts at: 1264 1624 1264
Size: 360 3479
RsrII CG'GwC_CG
Cuts at: 2034 2034
Size: 3839
SacI G_AGCT'C
Cuts at: 853 853
Size: 3839
SalI G'TCGA_C
Cuts at: 876 876
Size: 3839
SapI GCTCTTCn'nnn_
Cuts at: 1862 2072 1862
Size: 210 3629
SbfI CC_TGCA'GG
Cuts at: 886 886
Size: 3839
ScaI AGT'ACT
Cuts at: 3356 3356
Size: 3839
SfcI C'TryA_G
Cuts at: 33 452 882 1567 2560 2751 33
Size: 419 430 685 993 191 1121
Fragments arranged by size:
1121 993 685 430 419 191
SfoI GGC'GCC
Cuts at: 183 1271 1519 183
Size: 1088 248 2503
Fragments arranged by size:
2503 1088 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3839
SmaI CCC'GGG
Cuts at: 861 861
Size: 3839
SmlI C'TyrA_G
Cuts at: 826 994 1909 2401 2663 826
Size: 168 915 492 262 2002
Fragments arranged by size:
2002 915 492 262 168
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3839
SphI G_CATG'C
Cuts at: 892 1392 892
Size: 500 3339
StuI AGG'CCT
Cuts at: 606 606
Size: 3839
StyI C'CwwG_G
Cuts at: 107 563 973 1327 107
Size: 456 410 354 2619
Fragments arranged by size:
2619 456 410 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 446 1422 1555 2014 2223 446
Size: 976 133 459 209 2062
Fragments arranged by size:
2062 976 459 209 133
TatI w'GTAC_w
Cuts at: 3354 3354
Size: 3839
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 3807
Tth111I GACn'n_nGTC
Cuts at: 1636 1636
Size: 3839
XbaI T'CTAG_A
Cuts at: 870 870
Size: 3839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 801 1334 801
Size: 533 3306
XmaI C'CCGG_G
Cuts at: 859 859
Size: 3839
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI