Restriction map for
pF1KB7506
(Circular) (Six-base) MAPSORT of: pF1KB7506.seq Check: 4396 from: 1 to: 3833
pF1KB7506 3833 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 871 871
Size: 3833
Acc65I G'GTAC_C
Cuts at: 849 849
Size: 3833
AclI AA'CG_TT
Cuts at: 3673 3673
Size: 3833
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1658 2090 2837 3105 655
Size: 1003 432 747 268 1383
Fragments arranged by size:
1383 1003 747 432 268
AflIII A'CryG_T
Cuts at: 1185 2289 3266 1185
Size: 1104 977 1752
Fragments arranged by size:
1752 1104 977
AgeI A'CCGG_T
Cuts at: 2221 2221
Size: 3833
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 598 630 598
Size: 32 3801
AlwNI CAG_nnn'CTG
Cuts at: 531 1138 2705 531
Size: 607 1567 1659
Fragments arranged by size:
1659 1567 607
ApaI G_GGCC'C
Cuts at: 552 552
Size: 3833
ApaLI G'TGCA_C
Cuts at: 2603 2603
Size: 3833
ApoI r'AATT_y
Cuts at: 642 837 3186 3442 642
Size: 195 2349 256 1033
Fragments arranged by size:
2349 1033 256 195
AseI AT'TA_AT
Cuts at: 20 20
Size: 3833
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
AvaI C'yCGr_G
Cuts at: 382 541 853 382
Size: 159 312 3362
Fragments arranged by size:
3362 312 159
BamHI G'GATC_C
Cuts at: 858 858
Size: 3833
BanI G'GyrC_C
Cuts at: 431 849 1263 1511 1546 431
Size: 418 414 248 35 2718
Fragments arranged by size:
2718 418 414 248 35
BanII G_rGCy'C
Cuts at: 552 847 1877 552
Size: 295 1030 2508
Fragments arranged by size:
2508 1030 295
BbeI G_GCGC'C
Cuts at: 435 1267 1515 435
Size: 832 248 2753
Fragments arranged by size:
2753 832 248
BbsI GAAGACnn'nnnn_
Cuts at: 150 150
Size: 3833
BciVI GTATCCnnnnn_n'
Cuts at: 1731 2492 3833 1731
Size: 761 1341 1731
Fragments arranged by size:
1731 1341 761
BclI T'GATC_A
Cuts at: 1353 1353
Size: 3833
BglI GCCn_nnn'nGGC
Cuts at: 556 556
Size: 3833
BglII A'GATC_T
Cuts at: 593 1348 3385 593
Size: 755 2037 1041
Fragments arranged by size:
2037 1041 755
BlpI GC'TnA_GC
Cuts at: 945 945
Size: 3833
Bme1580I G_kGCm'C
Cuts at: 552 1458 1551 2607 552
Size: 906 93 1056 1778
Fragments arranged by size:
1778 1056 906 93
BmrI ACTGGGnnnn_n'
Cuts at: 1045 1126 1461 3605 1045
Size: 81 335 2144 1273
Fragments arranged by size:
2144 1273 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 408 2200 408
Size: 1792 2041
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 465 234
Size: 231 3602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1888 2380 2678 1009
Size: 879 492 298 2164
Fragments arranged by size:
2164 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3537 3537
Size: 3833
BsaAI yAC'GTr
Cuts at: 1186 1186
Size: 3833
BsaBI GATnn'nnATC
Cuts at: 6 1347 3384 6
Size: 1341 2037 455
Fragments arranged by size:
2037 1341 455
BsaHI Gr'CG_yC
Cuts at: 143 432 1264 1512 2214 3710 143
Size: 289 832 248 702 1496 266
Fragments arranged by size:
1496 832 702 289 266 248
BsaWI w'CCGG_w
Cuts at: 1022 1543 2221 2495 2642 3053 1022
Size: 521 678 274 147 411 1802
Fragments arranged by size:
1802 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 598 628 598
Size: 30 3803
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 786 786
Size: 3833
BseYI C'CCAG_C
Cuts at: 551 1258 2016 2593 3089 551
Size: 707 758 577 496 1295
Fragments arranged by size:
1295 758 707 577 496
BsiEI CG_ry'CG
Cuts at: 104 138 1375 1421 2629 104
Size: 34 1237 46 1208 1308
Fragments arranged by size:
1308 1237 1208 46 34
BsiHKAI G_wGCw'C
Cuts at: 371 847 1625 1815 2607 371
Size: 476 778 190 792 1597
Fragments arranged by size:
1597 792 778 476 190
Bsp1286I G_dGCh'C
Cuts at: 371 552 847 1458 1551 1625 1815 1877 2607
Size: 181 295 611 93 74 190 62 730
Cuts at: 2607 371
Size: 1597
Fragments arranged by size:
1597 730 611 295 190 181 93 74
62
BspEI T'CCGG_A
Cuts at: 3053 3053
Size: 3833
BspHI T'CATG_A
Cuts at: 3009 3009
Size: 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 369 883 1097 1399 1780 369
Size: 514 214 302 381 2422
Fragments arranged by size:
2422 514 381 302 214
BsrBI CCG'CTC
Cuts at: 50 429 2125 2179 3663 3828 50
Size: 379 1696 54 1484 165 55
Fragments arranged by size:
1696 1484 379 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1745 1745
Size: 3833
BsrFI r'CCGG_y
Cuts at: 1831 2012 2221 1831
Size: 181 209 3443
Fragments arranged by size:
3443 209 181
BssSI C'ACGA_G
Cuts at: 2104 2462 2104
Size: 358 3475
BstAPI GCAn_nnn'nTGC
Cuts at: 1210 1210
Size: 3833
BstBI TT'CG_AA
Cuts at: 2194 2194
Size: 3833
BstYI r'GATC_y
Cuts at: 1 593 723 858 1325 1348 1683 1929 2941
Size: 592 130 135 467 23 335 246 1012
Cuts at: 2941 3027 3039 3385 1
Size: 86 12 346 449
Fragments arranged by size:
1012 592 467 449 346 335 246 135
130 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 374 437 374
Size: 63 3770
BtgI C'CryG_G
Cuts at: 332 403 332
Size: 71 3762
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1783 1964 2127 1783
Size: 181 163 3489
Fragments arranged by size:
3489 181 163
BtsI GCAGTG_nn'
Cuts at: 1076 1076
Size: 3833
DraI TTT'AAA
Cuts at: 832 832
Size: 3833
DrdI GACnn_nn'nnGTC
Cuts at: 1539 2397 1539
Size: 858 2975
EaeI y'GGCC_r
Cuts at: 135 261 1418 1592 1983 2010 3355 135
Size: 126 1157 174 391 27 1345 613
Fragments arranged by size:
1345 1157 613 391 174 126 27
EagI C'GGCC_G
Cuts at: 135 1418 135
Size: 1283 2550
EarI CTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2041 2351 2497 3643 2041
Size: 310 146 1146 2231
Fragments arranged by size:
2231 1146 310 146
EcoICRI GAG'CTC
Cuts at: 845 845
Size: 3833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 465 655 1658 2090 2837 3105 234
Size: 231 190 1003 432 747 268 962
Fragments arranged by size:
1003 962 747 432 268 231 190
EcoNI CCTnn'n_nnAGG
Cuts at: 249 249
Size: 3833
EcoO109I rG'GnC_Cy
Cuts at: 441 548 677 740 972 441
Size: 107 129 63 232 3302
Fragments arranged by size:
3302 232 129 107 63
EcoRI G'AATT_C
Cuts at: 642 837 642
Size: 195 3638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1852 1852
Size: 3833
FspI TGC'GCA
Cuts at: 1614 1614
Size: 3833
HaeII r_GCGC'y
Cuts at: 340 435 538 1267 1515 2537 3516 340
Size: 95 103 729 248 1022 979 657
Fragments arranged by size:
1022 979 729 657 248 103 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1675 1705 1707 1737 1675
Size: 30 2 30 3771
Fragments arranged by size:
3771 30 30 2
HincII GTy'rAC
Cuts at: 872 872
Size: 3833
KasI G'GCGC_C
Cuts at: 431 1263 1511 431
Size: 832 248 2753
Fragments arranged by size:
2753 832 248
KpnI G_GTAC'C
Cuts at: 853 853
Size: 3833
MluI A'CGCG_T
Cuts at: 3266 3266
Size: 3833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2504 2688 2504
Size: 184 3649
MscI TGG'CCA
Cuts at: 263 1594 263
Size: 1331 2502
MslI CAynn'nnrTG
Cuts at: 1822 1949 1822
Size: 127 3706
MspA1I CmG'CkG
Cuts at: 334 405 492 555 944 1258 1618 2631 2876
Size: 71 87 63 389 314 360 1013 245
Cuts at: 2876 334
Size: 1291
Fragments arranged by size:
1291 1013 389 360 314 245 87 71
63
NaeI GCC'GGC
Cuts at: 2014 2014
Size: 3833
NarI GG'CG_CC
Cuts at: 432 1264 1512 432
Size: 832 248 2753
Fragments arranged by size:
2753 832 248
NgoMIV G'CCGG_C
Cuts at: 2012 2012
Size: 3833
NspI r_CATG'y
Cuts at: 886 1386 2293 886
Size: 500 907 2426
Fragments arranged by size:
2426 907 500
PasI CC'CwG_GG
Cuts at: 670 670
Size: 3833
PciI A'CATG_T
Cuts at: 2289 2289
Size: 3833
PmeI GTTT'AAAC
Cuts at: 832 832
Size: 3833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 598 630 2972 3004 598
Size: 32 2342 32 1427
Fragments arranged by size:
2342 1427 32 32
PpuMI rG'GwC_Cy
Cuts at: 677 740 677
Size: 63 3770
PspOMI G'GGCC_C
Cuts at: 548 548
Size: 3833
PspXI vC'TCGA_Gb
Cuts at: 382 382
Size: 3833
PstI C_TGCA'G
Cuts at: 366 491 497 880 1565 366
Size: 125 6 383 685 2634
Fragments arranged by size:
2634 685 383 125 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3833
PvuII CAG'CTG
Cuts at: 492 555 1258 1618 492
Size: 63 703 360 2707
Fragments arranged by size:
2707 703 360 63
RsrII CG'GwC_CG
Cuts at: 2028 2028
Size: 3833
SacI G_AGCT'C
Cuts at: 847 847
Size: 3833
SacII CC_GC'GG
Cuts at: 335 406 335
Size: 71 3762
SalI G'TCGA_C
Cuts at: 870 870
Size: 3833
SanDI GG'GwC_CC
Cuts at: 740 740
Size: 3833
SapI GCTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
SbfI CC_TGCA'GG
Cuts at: 880 880
Size: 3833
ScaI AGT'ACT
Cuts at: 3350 3350
Size: 3833
SfcI C'TryA_G
Cuts at: 33 362 487 493 876 1561 2554 2745 33
Size: 329 125 6 383 685 993 191 1121
Fragments arranged by size:
1121 993 685 383 329 191 125 6
SfoI GGC'GCC
Cuts at: 433 1265 1513 433
Size: 832 248 2753
Fragments arranged by size:
2753 832 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
SmaI CCC'GGG
Cuts at: 855 855
Size: 3833
SmlI C'TyrA_G
Cuts at: 382 988 1903 2395 2657 382
Size: 606 915 492 262 1558
Fragments arranged by size:
1558 915 606 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3833
SphI G_CATG'C
Cuts at: 886 1386 886
Size: 500 3333
StyI C'CwwG_G
Cuts at: 731 967 1321 731
Size: 236 354 3243
Fragments arranged by size:
3243 354 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 592 1416 1549 2008 2217 592
Size: 824 133 459 209 2208
Fragments arranged by size:
2208 824 459 209 133
TatI w'GTAC_w
Cuts at: 3348 3348
Size: 3833
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3350 3382 3350
Size: 32 3801
Tth111I GACn'n_nGTC
Cuts at: 1630 1630
Size: 3833
XbaI T'CTAG_A
Cuts at: 864 864
Size: 3833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1328 1328
Size: 3833
XhoI C'TCGA_G
Cuts at: 382 382
Size: 3833
XmaI C'CCGG_G
Cuts at: 853 853
Size: 3833
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PshAI PsiI PsrI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI