Restriction map for
pF1KB7444
(Circular) (Six-base) MAPSORT of: pF1KB7444.seq Check: 9969 from: 1 to: 4601
pF1KB7444 4601 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1329 1366 1329
Size: 37 4564
AccI GT'mk_AC
Cuts at: 1071 1639 1071
Size: 568 4033
Acc65I G'GTAC_C
Cuts at: 1617 1617
Size: 4601
AclI AA'CG_TT
Cuts at: 4441 4441
Size: 4601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 1273 2426 2858 3605 3873 407
Size: 866 1153 432 747 268 1135
Fragments arranged by size:
1153 1135 866 747 432 268
AflIII A'CryG_T
Cuts at: 1953 3057 4034 1953
Size: 1104 977 2520
Fragments arranged by size:
2520 1104 977
AgeI A'CCGG_T
Cuts at: 2989 2989
Size: 4601
AlwNI CAG_nnn'CTG
Cuts at: 1906 3473 1906
Size: 1567 3034
ApaLI G'TGCA_C
Cuts at: 1313 3371 1313
Size: 2058 2543
ApoI r'AATT_y
Cuts at: 1605 3954 4210 1605
Size: 2349 256 1996
Fragments arranged by size:
2349 1996 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4601
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
AvaI C'yCGr_G
Cuts at: 893 1004 1115 1413 1584 1621 893
Size: 111 111 298 171 37 3873
Fragments arranged by size:
3873 298 171 111 111 37
BamHI G'GATC_C
Cuts at: 1626 1626
Size: 4601
BanI G'GyrC_C
Cuts at: 491 1617 2031 2279 2314 491
Size: 1126 414 248 35 2778
Fragments arranged by size:
2778 1126 414 248 35
BanII G_rGCy'C
Cuts at: 1615 2645 1615
Size: 1030 3571
BbeI G_GCGC'C
Cuts at: 2035 2283 2035
Size: 248 4353
BciVI GTATCCnnnnn_n'
Cuts at: 502 976 1039 2499 3260 4601 502
Size: 474 63 1460 761 1341 502
Fragments arranged by size:
1460 1341 761 502 474 63
BclI T'GATC_A
Cuts at: 2121 2121
Size: 4601
BfrBI ATG'CAT
Cuts at: 823 934 1156 823
Size: 111 222 4268
Fragments arranged by size:
4268 222 111
BglII A'GATC_T
Cuts at: 436 2116 4153 436
Size: 1680 2037 884
Fragments arranged by size:
2037 1680 884
BlpI GC'TnA_GC
Cuts at: 1713 1713
Size: 4601
Bme1580I G_kGCm'C
Cuts at: 494 1317 2226 2319 3375 494
Size: 823 909 93 1056 1720
Fragments arranged by size:
1720 1056 909 823 93
BmrI ACTGGGnnnn_n'
Cuts at: 1813 1894 2229 4373 1813
Size: 81 335 2144 2041
Fragments arranged by size:
2144 2041 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1428 2968 1428
Size: 1540 3061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 735 735
Size: 4601
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1512 1777 2656 3148 3446 508
Size: 1004 265 879 492 298 1663
Fragments arranged by size:
1663 1004 879 492 298 265
BsaI GGTCTCn'nnnn_
Cuts at: 384 4305 384
Size: 3921 680
BsaAI yAC'GTr
Cuts at: 1954 1954
Size: 4601
BsaBI GATnn'nnATC
Cuts at: 6 767 2115 4152 6
Size: 761 1348 2037 455
Fragments arranged by size:
2037 1348 761 455
BsaHI Gr'CG_yC
Cuts at: 2032 2280 2982 4478 2032
Size: 248 702 1496 2155
Fragments arranged by size:
2155 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1790 2311 2989 3263 3410 3821 1790
Size: 521 678 274 147 411 2570
Fragments arranged by size:
2570 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 581 581
Size: 4601
BseYI C'CCAG_C
Cuts at: 2026 2784 3361 3857 2026
Size: 758 577 496 2770
Fragments arranged by size:
2770 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2143 2189 3397 104
Size: 2039 46 1208 1308
Fragments arranged by size:
2039 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1317 1499 1615 2393 2583 3375 1317
Size: 182 116 778 190 792 2543
Fragments arranged by size:
2543 792 778 190 182 116
BsmI GAATG_Cn'
Cuts at: 300 300
Size: 4601
Bsp1286I G_dGCh'C
Cuts at: 494 1317 1499 1615 2226 2319 2393 2583 2645
Size: 823 182 116 611 93 74 190 62
Cuts at: 2645 3375 494
Size: 730 1720
Fragments arranged by size:
1720 823 730 611 190 182 116 93
74 62
BspEI T'CCGG_A
Cuts at: 3821 3821
Size: 4601
BspHI T'CATG_A
Cuts at: 1215 3777 1215
Size: 2562 2039
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1329 1366 1651 1865 2167 2548 1329
Size: 37 285 214 302 381 3382
Fragments arranged by size:
3382 381 302 285 214 37
BsrBI CCG'CTC
Cuts at: 50 2893 2947 4431 4596 50
Size: 2843 54 1484 165 55
Fragments arranged by size:
2843 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2513 2513
Size: 4601
BsrFI r'CCGG_y
Cuts at: 2599 2780 2989 2599
Size: 181 209 4211
Fragments arranged by size:
4211 209 181
BssSI C'ACGA_G
Cuts at: 1379 1508 2872 3230 1379
Size: 129 1364 358 2750
Fragments arranged by size:
2750 1364 358 129
BstAPI GCAn_nnn'nTGC
Cuts at: 1321 1978 1321
Size: 657 3944
BstBI TT'CG_AA
Cuts at: 2962 2962
Size: 4601
BstYI r'GATC_y
Cuts at: 1 436 1626 2093 2116 2451 2697 3709 3795
Size: 435 1190 467 23 335 246 1012 86
Cuts at: 3795 3807 4153 1
Size: 12 346 449
Fragments arranged by size:
1190 1012 467 449 435 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1041 107
Size: 934 3667
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2551 2732 2895 2551
Size: 181 163 4257
Fragments arranged by size:
4257 181 163
BtsI GCAGTG_nn'
Cuts at: 459 1844 459
Size: 1385 3216
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1517 1552 1517
Size: 35 4566
DraI TTT'AAA
Cuts at: 1600 1600
Size: 4601
DrdI GACnn_nn'nnGTC
Cuts at: 2307 3165 2307
Size: 858 3743
EaeI y'GGCC_r
Cuts at: 2186 2360 2751 2778 4123 2186
Size: 174 391 27 1345 2664
Fragments arranged by size:
2664 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2186 2186
Size: 4601
EarI CTCTTCn'nnn_
Cuts at: 663 2624 2834 663
Size: 1961 210 2430
Fragments arranged by size:
2430 1961 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 402 1466 2809 3119 3265 4411 402
Size: 1064 1343 310 146 1146 592
Fragments arranged by size:
1343 1146 1064 592 310 146
EcoICRI GAG'CTC
Cuts at: 1613 1613
Size: 4601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 735 1273 2426 2858 3605 3873 407
Size: 328 538 1153 432 747 268 1135
Fragments arranged by size:
1153 1135 747 538 432 328 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1325 1325
Size: 4601
EcoO109I rG'GnC_Cy
Cuts at: 586 652 1233 1301 1330 1740 586
Size: 66 581 68 29 410 3447
Fragments arranged by size:
3447 581 410 68 66 29
EcoRI G'AATT_C
Cuts at: 1605 1605
Size: 4601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4601
FspI TGC'GCA
Cuts at: 2382 2382
Size: 4601
HaeII r_GCGC'y
Cuts at: 2035 2283 3305 4284 2035
Size: 248 1022 979 2352
Fragments arranged by size:
2352 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 216 248 708 715 740 747 1552 1584 2443
Size: 32 460 7 25 7 805 32 859
Cuts at: 2443 2473 2475 2505 216
Size: 30 2 30 2312
Fragments arranged by size:
2312 859 805 460 32 32 30 30
25 7 7 2
HincII GTy'rAC
Cuts at: 734 1072 1640 734
Size: 338 568 3695
Fragments arranged by size:
3695 568 338
HindIII A'AGCT_T
Cuts at: 133 133
Size: 4601
KasI G'GCGC_C
Cuts at: 2031 2279 2031
Size: 248 4353
KpnI G_GTAC'C
Cuts at: 1621 1621
Size: 4601
MluI A'CGCG_T
Cuts at: 4034 4034
Size: 4601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 616 3272 3456 616
Size: 2656 184 1761
Fragments arranged by size:
2656 1761 184
MscI TGG'CCA
Cuts at: 2362 2362
Size: 4601
MslI CAynn'nnrTG
Cuts at: 497 1262 1436 2590 2717 497
Size: 765 174 1154 127 2381
Fragments arranged by size:
2381 1154 765 174 127
MspA1I CmG'CkG
Cuts at: 1712 2026 2386 3399 3644 1712
Size: 314 360 1013 245 2669
Fragments arranged by size:
2669 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2782 2782
Size: 4601
NarI GG'CG_CC
Cuts at: 2032 2280 2032
Size: 248 4353
NcoI C'CATG_G
Cuts at: 107 1041 107
Size: 934 3667
NdeI CA'TA_TG
Cuts at: 1518 1518
Size: 4601
NgoMIV G'CCGG_C
Cuts at: 2780 2780
Size: 4601
NsiI A_TGCA'T
Cuts at: 825 936 1158 825
Size: 111 222 4268
Fragments arranged by size:
4268 222 111
NspI r_CATG'y
Cuts at: 732 1453 1654 2154 3061 732
Size: 721 201 500 907 2272
Fragments arranged by size:
2272 907 721 500 201
PciI A'CATG_T
Cuts at: 3057 3057
Size: 4601
PfoI T'CCnGG_A
Cuts at: 712 712
Size: 4601
PmeI GTTT'AAAC
Cuts at: 1600 1600
Size: 4601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 4569
PpuMI rG'GwC_Cy
Cuts at: 586 652 1301 586
Size: 66 649 3886
Fragments arranged by size:
3886 649 66
PspXI vC'TCGA_Gb
Cuts at: 1413 1584 1413
Size: 171 4430
PstI C_TGCA'G
Cuts at: 285 1648 2333 285
Size: 1363 685 2553
Fragments arranged by size:
2553 1363 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4601
PvuII CAG'CTG
Cuts at: 2026 2386 2026
Size: 360 4241
RsrII CG'GwC_CG
Cuts at: 2796 2796
Size: 4601
SacI G_AGCT'C
Cuts at: 1615 1615
Size: 4601
SalI G'TCGA_C
Cuts at: 1070 1638 1070
Size: 568 4033
SapI GCTCTTCn'nnn_
Cuts at: 2624 2834 2624
Size: 210 4391
SbfI CC_TGCA'GG
Cuts at: 1648 1648
Size: 4601
ScaI AGT'ACT
Cuts at: 4118 4118
Size: 4601
SfcI C'TryA_G
Cuts at: 33 281 1544 1644 2329 3322 3513 33
Size: 248 1263 100 685 993 191 1121
Fragments arranged by size:
1263 1121 993 685 248 191 100
SfoI GGC'GCC
Cuts at: 2033 2281 2033
Size: 248 4353
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
SmaI CCC'GGG
Cuts at: 1623 1623
Size: 4601
SmlI C'TyrA_G
Cuts at: 523 1413 1527 1584 1756 2671 3163 3425 523
Size: 890 114 57 172 915 492 262 1699
Fragments arranged by size:
1699 915 890 492 262 172 114 57
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4601
SphI G_CATG'C
Cuts at: 1654 2154 1654
Size: 500 4101
StyI C'CwwG_G
Cuts at: 107 648 1041 1236 1735 2089 107
Size: 541 393 195 499 354 2619
Fragments arranged by size:
2619 541 499 393 354 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2184 2317 2776 2985 2184
Size: 133 459 209 3800
Fragments arranged by size:
3800 459 209 133
TatI w'GTAC_w
Cuts at: 4116 4116
Size: 4601
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 4569
Tth111I GACn'n_nGTC
Cuts at: 2398 2398
Size: 4601
XbaI T'CTAG_A
Cuts at: 811 865 922 976 1033 1087 1198 1632 811
Size: 54 57 54 57 54 111 434 3780
Fragments arranged by size:
3780 434 111 57 57 54 54 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2096 2096
Size: 4601
XhoI C'TCGA_G
Cuts at: 1413 1584 1413
Size: 171 4430
XmaI C'CCGG_G
Cuts at: 1621 1621
Size: 4601
XmnI GAAnn'nnTTC
Cuts at: 420 420
Size: 4601
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
ZraI