Restriction map for
pF1KB7421
(Circular) (Six-base) MAPSORT of: pF1KB7421.seq Check: 7553 from: 1 to: 4220
pF1KB7421 4220 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1258 1258
Size: 4220
Acc65I G'GTAC_C
Cuts at: 1236 1236
Size: 4220
AclI AA'CG_TT
Cuts at: 4060 4060
Size: 4220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1180 2045 2477 3224 3492 832
Size: 348 865 432 747 268 1560
Fragments arranged by size:
1560 865 747 432 348 268
AflIII A'CryG_T
Cuts at: 180 1572 2676 3653 180
Size: 1392 1104 977 747
Fragments arranged by size:
1392 1104 977 747
AgeI A'CCGG_T
Cuts at: 2608 2608
Size: 4220
AleI CACnn'nnGTG
Cuts at: 487 487
Size: 4220
AlwNI CAG_nnn'CTG
Cuts at: 1525 3092 1525
Size: 1567 2653
ApaLI G'TGCA_C
Cuts at: 546 2990 546
Size: 2444 1776
ApoI r'AATT_y
Cuts at: 589 1224 3573 3829 589
Size: 635 2349 256 980
Fragments arranged by size:
2349 980 635 256
AseI AT'TA_AT
Cuts at: 20 173 20
Size: 153 4067
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
AvaI C'yCGr_G
Cuts at: 770 1240 770
Size: 470 3750
BamHI G'GATC_C
Cuts at: 1245 1245
Size: 4220
BanI G'GyrC_C
Cuts at: 798 1236 1650 1898 1933 798
Size: 438 414 248 35 3085
Fragments arranged by size:
3085 438 414 248 35
BanII G_rGCy'C
Cuts at: 715 946 1158 1234 2264 715
Size: 231 212 76 1030 2671
Fragments arranged by size:
2671 1030 231 212 76
BbeI G_GCGC'C
Cuts at: 1654 1902 1654
Size: 248 3972
BbvCI CC'TCA_GC
Cuts at: 445 445
Size: 4220
BciVI GTATCCnnnnn_n'
Cuts at: 2118 2879 4220 2118
Size: 761 1341 2118
Fragments arranged by size:
2118 1341 761
BclI T'GATC_A
Cuts at: 1740 1740
Size: 4220
BglII A'GATC_T
Cuts at: 1735 3772 1735
Size: 2037 2183
BlpI GC'TnA_GC
Cuts at: 1332 1332
Size: 4220
Bme1580I G_kGCm'C
Cuts at: 550 1845 1938 2994 550
Size: 1295 93 1056 1776
Fragments arranged by size:
1776 1295 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1432 1513 1848 3992 1432
Size: 81 335 2144 1660
Fragments arranged by size:
2144 1660 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2587 2587
Size: 4220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1172 1172
Size: 4220
Bpu10I CC'TnA_GC
Cuts at: 445 445
Size: 4220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 2275 2767 3065 1396
Size: 879 492 298 2551
Fragments arranged by size:
2551 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3924 3924
Size: 4220
BsaAI yAC'GTr
Cuts at: 1573 1573
Size: 4220
BsaBI GATnn'nnATC
Cuts at: 6 1734 3771 6
Size: 1728 2037 455
Fragments arranged by size:
2037 1728 455
BsaHI Gr'CG_yC
Cuts at: 1651 1899 2601 4097 1651
Size: 248 702 1496 1774
Fragments arranged by size:
1774 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1409 1930 2608 2882 3029 3440 1409
Size: 521 678 274 147 411 2189
Fragments arranged by size:
2189 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 433 433
Size: 4220
BseYI C'CCAG_C
Cuts at: 600 1645 2403 2980 3476 600
Size: 1045 758 577 496 1344
Fragments arranged by size:
1344 1045 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 845 845
Size: 4220
BsiEI CG_ry'CG
Cuts at: 104 1762 1808 3016 104
Size: 1658 46 1208 1308
Fragments arranged by size:
1658 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 550 946 1158 1234 2012 2202 2994 550
Size: 396 212 76 778 190 792 1776
Fragments arranged by size:
1776 792 778 396 212 190 76
BsmI GAATG_Cn'
Cuts at: 211 211
Size: 4220
Bsp1286I G_dGCh'C
Cuts at: 550 715 946 1158 1234 1845 1938 2012 2202
Size: 165 231 212 76 611 93 74 190
Cuts at: 2202 2264 2994 550
Size: 62 730 1776
Fragments arranged by size:
1776 730 611 231 212 190 165 93
76 74 62
BspEI T'CCGG_A
Cuts at: 3440 3440
Size: 4220
BspHI T'CATG_A
Cuts at: 662 3396 662
Size: 2734 1486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 521 685 1270 1484 1786 2167 521
Size: 164 585 214 302 381 2574
Fragments arranged by size:
2574 585 381 302 214 164
BsrBI CCG'CTC
Cuts at: 50 2512 2566 4050 4215 50
Size: 2462 54 1484 165 55
Fragments arranged by size:
2462 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2132 2132
Size: 4220
BsrFI r'CCGG_y
Cuts at: 2218 2399 2608 2218
Size: 181 209 3830
Fragments arranged by size:
3830 209 181
BsrGI T'GTAC_A
Cuts at: 1025 1025
Size: 4220
BssSI C'ACGA_G
Cuts at: 2491 2849 2491
Size: 358 3862
BstAPI GCAn_nnn'nTGC
Cuts at: 1597 1597
Size: 4220
BstBI TT'CG_AA
Cuts at: 2581 2581
Size: 4220
BstYI r'GATC_y
Cuts at: 1 1245 1712 1735 2070 2316 3328 3414 3426
Size: 1244 467 23 335 246 1012 86 12
Cuts at: 3426 3772 1
Size: 346 449
Fragments arranged by size:
1244 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 327 327
Size: 4220
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2170 2351 2514 2170
Size: 181 163 3876
Fragments arranged by size:
3876 181 163
BtsI GCAGTG_nn'
Cuts at: 1463 1463
Size: 4220
DraI TTT'AAA
Cuts at: 131 152 1219 131
Size: 21 1067 3132
Fragments arranged by size:
3132 1067 21
DrdI GACnn_nn'nnGTC
Cuts at: 999 1926 2784 999
Size: 927 858 2435
Fragments arranged by size:
2435 927 858
EaeI y'GGCC_r
Cuts at: 377 629 1805 1979 2370 2397 3742 377
Size: 252 1176 174 391 27 1345 855
Fragments arranged by size:
1345 1176 855 391 252 174 27
EagI C'GGCC_G
Cuts at: 1805 1805
Size: 4220
EarI CTCTTCn'nnn_
Cuts at: 260 1156 2243 2453 260
Size: 896 1087 210 2027
Fragments arranged by size:
2027 1087 896 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2428 2738 2884 4030 2428
Size: 310 146 1146 2618
Fragments arranged by size:
2618 1146 310 146
EcoICRI GAG'CTC
Cuts at: 944 1156 1232 944
Size: 212 76 3932
Fragments arranged by size:
3932 212 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1172 1180 2045 2477 3224 3492 832
Size: 340 8 865 432 747 268 1560
Fragments arranged by size:
1560 865 747 432 340 268 8
EcoNI CCTnn'n_nnAGG
Cuts at: 1173 1173
Size: 4220
EcoO109I rG'GnC_Cy
Cuts at: 1166 1186 1359 1166
Size: 20 173 4027
Fragments arranged by size:
4027 173 20
EcoRI G'AATT_C
Cuts at: 589 1224 589
Size: 635 3585
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1185 2239 1185
Size: 1054 3166
FspI TGC'GCA
Cuts at: 2001 2001
Size: 4220
HaeII r_GCGC'y
Cuts at: 1654 1902 2924 3903 1654
Size: 248 1022 979 1971
Fragments arranged by size:
1971 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 641 673 1140 1172 2062 2092 2094 2124 641
Size: 32 467 32 890 30 2 30 2737
Fragments arranged by size:
2737 890 467 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1259 1259
Size: 4220
KasI G'GCGC_C
Cuts at: 1650 1898 1650
Size: 248 3972
KpnI G_GTAC'C
Cuts at: 1240 1240
Size: 4220
MluI A'CGCG_T
Cuts at: 3653 3653
Size: 4220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 661 2891 3075 661
Size: 2230 184 1806
Fragments arranged by size:
2230 1806 184
MscI TGG'CCA
Cuts at: 379 631 1981 379
Size: 252 1350 2618
Fragments arranged by size:
2618 1350 252
MslI CAynn'nnrTG
Cuts at: 298 487 745 994 1033 1105 2209 2336 298
Size: 189 258 249 39 72 1104 127 2182
Fragments arranged by size:
2182 1104 258 249 189 127 72 39
MspA1I CmG'CkG
Cuts at: 1331 1645 2005 3018 3263 1331
Size: 314 360 1013 245 2288
Fragments arranged by size:
2288 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2401 2401
Size: 4220
NarI GG'CG_CC
Cuts at: 1651 1899 1651
Size: 248 3972
NgoMIV G'CCGG_C
Cuts at: 2399 2399
Size: 4220
NspI r_CATG'y
Cuts at: 184 824 1038 1273 1773 2680 184
Size: 640 214 235 500 907 1724
Fragments arranged by size:
1724 907 640 500 235 214
PasI CC'CwG_GG
Cuts at: 200 200
Size: 4220
PciI A'CATG_T
Cuts at: 180 2676 180
Size: 2496 1724
PmeI GTTT'AAAC
Cuts at: 1219 1219
Size: 4220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3359 3391 3359
Size: 32 4188
PpuMI rG'GwC_Cy
Cuts at: 1186 1186
Size: 4220
PstI C_TGCA'G
Cuts at: 1267 1952 1267
Size: 685 3535
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4220
PvuII CAG'CTG
Cuts at: 1645 2005 1645
Size: 360 3860
RsrII CG'GwC_CG
Cuts at: 2415 2415
Size: 4220
SacI G_AGCT'C
Cuts at: 946 1158 1234 946
Size: 212 76 3932
Fragments arranged by size:
3932 212 76
SalI G'TCGA_C
Cuts at: 1257 1257
Size: 4220
SapI GCTCTTCn'nnn_
Cuts at: 2243 2453 2243
Size: 210 4010
SbfI CC_TGCA'GG
Cuts at: 1267 1267
Size: 4220
ScaI AGT'ACT
Cuts at: 3737 3737
Size: 4220
SfcI C'TryA_G
Cuts at: 33 251 284 893 926 1125 1263 1948 2941
Size: 218 33 609 33 199 138 685 993
Cuts at: 2941 3132 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 609 218 199 191 138
33 33
SfoI GGC'GCC
Cuts at: 1652 1900 1652
Size: 248 3972
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
SmaI CCC'GGG
Cuts at: 772 1242 772
Size: 470 3750
SmlI C'TyrA_G
Cuts at: 1375 2290 2782 3044 1375
Size: 915 492 262 2551
Fragments arranged by size:
2551 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4220
SphI G_CATG'C
Cuts at: 1273 1773 1273
Size: 500 3720
StyI C'CwwG_G
Cuts at: 1169 1354 1708 1169
Size: 185 354 3681
Fragments arranged by size:
3681 354 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 444 1119 1803 1936 2395 2604 444
Size: 675 684 133 459 209 2060
Fragments arranged by size:
2060 684 675 459 209 133
TatI w'GTAC_w
Cuts at: 269 434 1025 3735 269
Size: 165 591 2710 754
Fragments arranged by size:
2710 754 591 165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 791 823 3737 3769 791
Size: 32 2914 32 1242
Fragments arranged by size:
2914 1242 32 32
Tth111I GACn'n_nGTC
Cuts at: 1072 2017 1072
Size: 945 3275
XbaI T'CTAG_A
Cuts at: 1251 1251
Size: 4220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 537 1715 537
Size: 1178 3042
XmaI C'CCGG_G
Cuts at: 770 1240 770
Size: 470 3750
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI