Restriction map for
pF1KB7417
(Circular) (Six-base) MAPSORT of: pF1KB7417.seq Check: 1283 from: 1 to: 4208
pF1KB7417 4208 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 580 580
Size: 4208
AccI GT'mk_AC
Cuts at: 1246 1246
Size: 4208
Acc65I G'GTAC_C
Cuts at: 1224 1224
Size: 4208
AclI AA'CG_TT
Cuts at: 4048 4048
Size: 4208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 2033 2465 3212 3480 581
Size: 1452 432 747 268 1309
Fragments arranged by size:
1452 1309 747 432 268
AflIII A'CryG_T
Cuts at: 1560 2664 3641 1560
Size: 1104 977 2127
Fragments arranged by size:
2127 1104 977
AgeI A'CCGG_T
Cuts at: 2596 2596
Size: 4208
AleI CACnn'nnGTG
Cuts at: 318 412 695 1180 318
Size: 94 283 485 3346
Fragments arranged by size:
3346 485 283 94
AlwNI CAG_nnn'CTG
Cuts at: 1513 3080 1513
Size: 1567 2641
ApaLI G'TGCA_C
Cuts at: 2978 2978
Size: 4208
ApoI r'AATT_y
Cuts at: 1212 3561 3817 1212
Size: 2349 256 1603
Fragments arranged by size:
2349 1603 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4208
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
AvaI C'yCGr_G
Cuts at: 1128 1228 1128
Size: 100 4108
BamHI G'GATC_C
Cuts at: 1233 1233
Size: 4208
BanI G'GyrC_C
Cuts at: 625 1224 1638 1886 1921 625
Size: 599 414 248 35 2912
Fragments arranged by size:
2912 599 414 248 35
BanII G_rGCy'C
Cuts at: 1222 2252 1222
Size: 1030 3178
BbeI G_GCGC'C
Cuts at: 1642 1890 1642
Size: 248 3960
BbsI GAAGACnn'nnnn_
Cuts at: 780 780
Size: 4208
BciVI GTATCCnnnnn_n'
Cuts at: 128 2106 2867 4208 128
Size: 1978 761 1341 128
Fragments arranged by size:
1978 1341 761 128
BclI T'GATC_A
Cuts at: 1728 1728
Size: 4208
BglII A'GATC_T
Cuts at: 1723 3760 1723
Size: 2037 2171
BlpI GC'TnA_GC
Cuts at: 1320 1320
Size: 4208
Bme1580I G_kGCm'C
Cuts at: 530 1833 1926 2982 530
Size: 1303 93 1056 1756
Fragments arranged by size:
1756 1303 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 689 1420 1501 1836 3980 689
Size: 731 81 335 2144 917
Fragments arranged by size:
2144 917 731 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1081 2575 1081
Size: 1494 2714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 738 1105 526
Size: 212 367 3629
Fragments arranged by size:
3629 367 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 1384 2263 2755 3053 449
Size: 935 879 492 298 1604
Fragments arranged by size:
1604 935 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 845 3912 845
Size: 3067 1141
BsaAI yAC'GTr
Cuts at: 473 1164 1561 473
Size: 691 397 3120
Fragments arranged by size:
3120 691 397
BsaBI GATnn'nnATC
Cuts at: 6 1722 3759 6
Size: 1716 2037 455
Fragments arranged by size:
2037 1716 455
BsaHI Gr'CG_yC
Cuts at: 1639 1887 2589 4085 1639
Size: 248 702 1496 1762
Fragments arranged by size:
1762 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1397 1918 2596 2870 3017 3428 1397
Size: 521 678 274 147 411 2177
Fragments arranged by size:
2177 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 763 793 1141 1171 763
Size: 30 348 30 3800
Fragments arranged by size:
3800 348 30 30
BseYI C'CCAG_C
Cuts at: 1168 1633 2391 2968 3464 1168
Size: 465 758 577 496 1912
Fragments arranged by size:
1912 758 577 496 465
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1110 1204 1110
Size: 94 4114
BsiEI CG_ry'CG
Cuts at: 104 1750 1796 3004 104
Size: 1646 46 1208 1308
Fragments arranged by size:
1646 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1222 2000 2190 2982 1222
Size: 778 190 792 2448
Fragments arranged by size:
2448 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 530 1222 1833 1926 2000 2190 2252 2982 530
Size: 692 611 93 74 190 62 730 1756
Fragments arranged by size:
1756 730 692 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3428 3428
Size: 4208
BspHI T'CATG_A
Cuts at: 3384 3384
Size: 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 580 1258 1472 1774 2155 580
Size: 678 214 302 381 2633
Fragments arranged by size:
2633 678 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2500 2554 4038 4203 50
Size: 2450 54 1484 165 55
Fragments arranged by size:
2450 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1110 2120 1110
Size: 1010 3198
BsrFI r'CCGG_y
Cuts at: 2206 2387 2596 2206
Size: 181 209 3818
Fragments arranged by size:
3818 209 181
BssSI C'ACGA_G
Cuts at: 2479 2837 2479
Size: 358 3850
BstAPI GCAn_nnn'nTGC
Cuts at: 1121 1585 1121
Size: 464 3744
BstBI TT'CG_AA
Cuts at: 2569 2569
Size: 4208
BstXI CCAn_nnnn'nTGG
Cuts at: 1168 1168
Size: 4208
BstYI r'GATC_y
Cuts at: 1 389 1233 1700 1723 2058 2304 3316 3402
Size: 388 844 467 23 335 246 1012 86
Cuts at: 3402 3414 3760 1
Size: 12 346 449
Fragments arranged by size:
1012 844 467 449 388 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 556 556
Size: 4208
BtgI C'CryG_G
Cuts at: 107 107
Size: 4208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2158 2339 2502 2158
Size: 181 163 3864
Fragments arranged by size:
3864 181 163
BtsI GCAGTG_nn'
Cuts at: 1098 1451 1098
Size: 353 3855
DraI TTT'AAA
Cuts at: 1207 1207
Size: 4208
DrdI GACnn_nn'nnGTC
Cuts at: 1914 2772 1914
Size: 858 3350
EaeI y'GGCC_r
Cuts at: 1793 1967 2358 2385 3730 1793
Size: 174 391 27 1345 2271
Fragments arranged by size:
2271 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1793 1793
Size: 4208
EarI CTCTTCn'nnn_
Cuts at: 2231 2441 2231
Size: 210 3998
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2416 2726 2872 4018 2416
Size: 310 146 1146 2606
Fragments arranged by size:
2606 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1220 1220
Size: 4208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 581 738 1105 2033 2465 3212 3480 526
Size: 55 157 367 928 432 747 268 1254
Fragments arranged by size:
1254 928 747 432 367 268 157 55
EcoNI CCTnn'n_nnAGG
Cuts at: 505 505
Size: 4208
EcoO109I rG'GnC_Cy
Cuts at: 1347 1347
Size: 4208
EcoRI G'AATT_C
Cuts at: 1212 1212
Size: 4208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 4208
FspI TGC'GCA
Cuts at: 1989 1989
Size: 4208
HaeII r_GCGC'y
Cuts at: 1191 1642 1890 2912 3891 1191
Size: 451 248 1022 979 1508
Fragments arranged by size:
1508 1022 979 451 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1092 1124 1141 1173 2050 2080 2082 2112 1092
Size: 32 17 32 877 30 2 30 3188
Fragments arranged by size:
3188 877 32 32 30 30 17 2
HincII GTy'rAC
Cuts at: 185 1247 185
Size: 1062 3146
KasI G'GCGC_C
Cuts at: 1638 1886 1638
Size: 248 3960
KpnI G_GTAC'C
Cuts at: 1228 1228
Size: 4208
MluI A'CGCG_T
Cuts at: 3641 3641
Size: 4208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1001 2879 3063 1001
Size: 1878 184 2146
Fragments arranged by size:
2146 1878 184
MscI TGG'CCA
Cuts at: 1969 1969
Size: 4208
MslI CAynn'nnrTG
Cuts at: 318 412 695 1180 2197 2324 318
Size: 94 283 485 1017 127 2202
Fragments arranged by size:
2202 1017 485 283 127 94
MspA1I CmG'CkG
Cuts at: 492 1319 1633 1993 3006 3251 492
Size: 827 314 360 1013 245 1449
Fragments arranged by size:
1449 1013 827 360 314 245
NaeI GCC'GGC
Cuts at: 2389 2389
Size: 4208
NarI GG'CG_CC
Cuts at: 1639 1887 1639
Size: 248 3960
NcoI C'CATG_G
Cuts at: 107 107
Size: 4208
NdeI CA'TA_TG
Cuts at: 887 887
Size: 4208
NgoMIV G'CCGG_C
Cuts at: 2387 2387
Size: 4208
NspI r_CATG'y
Cuts at: 1261 1761 2668 1261
Size: 500 907 2801
Fragments arranged by size:
2801 907 500
PciI A'CATG_T
Cuts at: 2664 2664
Size: 4208
PflMI CCAn_nnn'nTGG
Cuts at: 840 840
Size: 4208
PmeI GTTT'AAAC
Cuts at: 1207 1207
Size: 4208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 4176
PstI C_TGCA'G
Cuts at: 481 1255 1940 481
Size: 774 685 2749
Fragments arranged by size:
2749 774 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4208
PvuII CAG'CTG
Cuts at: 492 1633 1993 492
Size: 1141 360 2707
Fragments arranged by size:
2707 1141 360
RsrII CG'GwC_CG
Cuts at: 2403 2403
Size: 4208
SacI G_AGCT'C
Cuts at: 1222 1222
Size: 4208
SalI G'TCGA_C
Cuts at: 1245 1245
Size: 4208
SapI GCTCTTCn'nnn_
Cuts at: 2231 2441 2231
Size: 210 3998
SbfI CC_TGCA'GG
Cuts at: 1255 1255
Size: 4208
ScaI AGT'ACT
Cuts at: 3725 3725
Size: 4208
SexAI A'CCwGG_T
Cuts at: 632 632
Size: 4208
SfcI C'TryA_G
Cuts at: 33 477 1251 1936 2929 3120 33
Size: 444 774 685 993 191 1121
Fragments arranged by size:
1121 993 774 685 444 191
SfoI GGC'GCC
Cuts at: 1640 1888 1640
Size: 248 3960
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
SmaI CCC'GGG
Cuts at: 1130 1230 1130
Size: 100 4108
SmlI C'TyrA_G
Cuts at: 464 1363 2278 2770 3032 464
Size: 899 915 492 262 1640
Fragments arranged by size:
1640 915 899 492 262
SnaBI TAC'GTA
Cuts at: 473 473
Size: 4208
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4208
SphI G_CATG'C
Cuts at: 1261 1761 1261
Size: 500 3708
SspI AAT'ATT
Cuts at: 298 933 298
Size: 635 3573
StuI AGG'CCT
Cuts at: 879 901 879
Size: 22 4186
StyI C'CwwG_G
Cuts at: 107 276 732 1342 1696 107
Size: 169 456 610 354 2619
Fragments arranged by size:
2619 610 456 354 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 411 424 1791 1924 2383 2592 411
Size: 13 1367 133 459 209 2027
Fragments arranged by size:
2027 1367 459 209 133 13
TatI w'GTAC_w
Cuts at: 569 836 3723 569
Size: 267 2887 1054
Fragments arranged by size:
2887 1054 267
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 132 164 1164 1196 3725 3757 132
Size: 32 1000 32 2529 32 583
Fragments arranged by size:
2529 1000 583 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2005 2005
Size: 4208
XbaI T'CTAG_A
Cuts at: 1239 1239
Size: 4208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 582 1703 582
Size: 1121 3087
XmaI C'CCGG_G
Cuts at: 1128 1228 1128
Size: 100 4108
XmnI GAAnn'nnTTC
Cuts at: 286 933 286
Size: 647 3561
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI SwaI XhoI ZraI