Restriction map for
pF1KB7400
(Circular) (Six-base) MAPSORT of: pF1KB7400.seq Check: 2379 from: 1 to: 4028
pF1KB7400 4028 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 332 1066 332
Size: 734 3294
Acc65I G'GTAC_C
Cuts at: 1044 1044
Size: 4028
AclI AA'CG_TT
Cuts at: 592 3868 592
Size: 3276 752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 1853 2285 3032 3300 386
Size: 1467 432 747 268 1114
Fragments arranged by size:
1467 1114 747 432 268
AflIII A'CryG_T
Cuts at: 1380 2484 3461 1380
Size: 1104 977 1947
Fragments arranged by size:
1947 1104 977
AgeI A'CCGG_T
Cuts at: 2416 2416
Size: 4028
AlwNI CAG_nnn'CTG
Cuts at: 133 1333 2900 133
Size: 1200 1567 1261
Fragments arranged by size:
1567 1261 1200
ApaLI G'TGCA_C
Cuts at: 2798 2798
Size: 4028
ApoI r'AATT_y
Cuts at: 1032 3381 3637 1032
Size: 2349 256 1423
Fragments arranged by size:
2349 1423 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 1048 1048
Size: 4028
BamHI G'GATC_C
Cuts at: 1053 1053
Size: 4028
BanI G'GyrC_C
Cuts at: 1044 1458 1706 1741 1044
Size: 414 248 35 3331
Fragments arranged by size:
3331 414 248 35
BanII G_rGCy'C
Cuts at: 1042 2072 1042
Size: 1030 2998
BbeI G_GCGC'C
Cuts at: 1462 1710 1462
Size: 248 3780
BbsI GAAGACnn'nnnn_
Cuts at: 374 374
Size: 4028
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 222 256 90
Size: 34 98 34 3862
Fragments arranged by size:
3862 98 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2687 4028 1926
Size: 761 1341 1926
Fragments arranged by size:
1926 1341 761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4028
BglII A'GATC_T
Cuts at: 892 1543 3580 892
Size: 651 2037 1340
Fragments arranged by size:
2037 1340 651
BlpI GC'TnA_GC
Cuts at: 1140 1140
Size: 4028
Bme1580I G_kGCm'C
Cuts at: 1653 1746 2802 1653
Size: 93 1056 2879
Fragments arranged by size:
2879 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1015 1240 1321 1656 3800 1015
Size: 225 81 335 2144 1243
Fragments arranged by size:
2144 1243 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 801 1204 2083 2575 2873 801
Size: 403 879 492 298 1956
Fragments arranged by size:
1956 879 492 403 298
BsaI GGTCTCn'nnnn_
Cuts at: 3732 3732
Size: 4028
BsaAI yAC'GTr
Cuts at: 474 1381 474
Size: 907 3121
BsaBI GATnn'nnATC
Cuts at: 6 1542 3579 6
Size: 1536 2037 455
Fragments arranged by size:
2037 1536 455
BsaHI Gr'CG_yC
Cuts at: 1459 1707 2409 3905 1459
Size: 248 702 1496 1582
Fragments arranged by size:
1582 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1217 1738 2416 2690 2837 3248 1217
Size: 521 678 274 147 411 1997
Fragments arranged by size:
1997 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 847 877 847
Size: 30 3998
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 129 780 783 819 129
Size: 651 3 36 3338
Fragments arranged by size:
3338 651 36 3
BseYI C'CCAG_C
Cuts at: 147 177 1453 2211 2788 3284 147
Size: 30 1276 758 577 496 891
Fragments arranged by size:
1276 891 758 577 496 30
BsiEI CG_ry'CG
Cuts at: 104 1570 1616 2824 104
Size: 1466 46 1208 1308
Fragments arranged by size:
1466 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 743 1042 1820 2010 2802 743
Size: 299 778 190 792 1969
Fragments arranged by size:
1969 792 778 299 190
BsmI GAATG_Cn'
Cuts at: 176 176
Size: 4028
Bsp1286I G_dGCh'C
Cuts at: 743 1042 1653 1746 1820 2010 2072 2802 743
Size: 299 611 93 74 190 62 730 1969
Fragments arranged by size:
1969 730 611 299 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3248 3248
Size: 4028
BspHI T'CATG_A
Cuts at: 965 3204 965
Size: 2239 1789
BspMI ACCTGCnnnn'nnnn_
Cuts at: 364 452 1078 1292 1594 1975 364
Size: 88 626 214 302 381 2417
Fragments arranged by size:
2417 626 381 302 214 88
BsrBI CCG'CTC
Cuts at: 50 2320 2374 3858 4023 50
Size: 2270 54 1484 165 55
Fragments arranged by size:
2270 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 529 1940 529
Size: 1411 2617
BsrFI r'CCGG_y
Cuts at: 2026 2207 2416 2026
Size: 181 209 3638
Fragments arranged by size:
3638 209 181
BssSI C'ACGA_G
Cuts at: 2299 2657 2299
Size: 358 3670
BstAPI GCAn_nnn'nTGC
Cuts at: 1405 1405
Size: 4028
BstBI TT'CG_AA
Cuts at: 2389 2389
Size: 4028
BstYI r'GATC_y
Cuts at: 1 312 344 785 892 1053 1520 1543 1878
Size: 311 32 441 107 161 467 23 335
Cuts at: 1878 2124 3136 3222 3234 3580 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 441 346 335 311 246
161 107 86 32 23 12
Bsu36I CC'TnA_GG
Cuts at: 979 979
Size: 4028
BtgI C'CryG_G
Cuts at: 455 455
Size: 4028
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1978 2159 2322 1978
Size: 181 163 3684
Fragments arranged by size:
3684 181 163
BtsI GCAGTG_nn'
Cuts at: 743 1271 743
Size: 528 3500
DraI TTT'AAA
Cuts at: 1027 1027
Size: 4028
DrdI GACnn_nn'nnGTC
Cuts at: 1734 2592 1734
Size: 858 3170
EaeI y'GGCC_r
Cuts at: 476 1613 1787 2178 2205 3550 476
Size: 1137 174 391 27 1345 954
Fragments arranged by size:
1345 1137 954 391 174 27
EagI C'GGCC_G
Cuts at: 1613 1613
Size: 4028
EarI CTCTTCn'nnn_
Cuts at: 190 576 2051 2261 190
Size: 386 1475 210 1957
Fragments arranged by size:
1957 1475 386 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2236 2546 2692 3838 2236
Size: 310 146 1146 2426
Fragments arranged by size:
2426 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1040 1040
Size: 4028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 1853 2285 3032 3300 386
Size: 1467 432 747 268 1114
Fragments arranged by size:
1467 1114 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1167 1167
Size: 4028
EcoRI G'AATT_C
Cuts at: 1032 1032
Size: 4028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 4028
FspI TGC'GCA
Cuts at: 1809 1809
Size: 4028
HaeII r_GCGC'y
Cuts at: 1462 1710 2732 3711 1462
Size: 248 1022 979 1779
Fragments arranged by size:
1779 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 1870 1900 1902 1932 105
Size: 32 1733 30 2 30 2201
Fragments arranged by size:
2201 1733 32 30 30 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 1458 1706 1458
Size: 248 3780
KpnI G_GTAC'C
Cuts at: 1048 1048
Size: 4028
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2699 2883 2699
Size: 184 3844
MscI TGG'CCA
Cuts at: 478 1789 478
Size: 1311 2717
MslI CAynn'nnrTG
Cuts at: 277 2017 2144 277
Size: 1740 127 2161
Fragments arranged by size:
2161 1740 127
MspA1I CmG'CkG
Cuts at: 177 1139 1453 1813 2826 3071 177
Size: 962 314 360 1013 245 1134
Fragments arranged by size:
1134 1013 962 360 314 245
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 4028
NarI GG'CG_CC
Cuts at: 1459 1707 1459
Size: 248 3780
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 4028
NspI r_CATG'y
Cuts at: 827 1081 1581 2488 827
Size: 254 500 907 2367
Fragments arranged by size:
2367 907 500 254
PciI A'CATG_T
Cuts at: 2484 2484
Size: 4028
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3996
PshAI GACnn'nnGTC
Cuts at: 844 844
Size: 4028
PstI C_TGCA'G
Cuts at: 420 1075 1760 420
Size: 655 685 2688
Fragments arranged by size:
2688 685 655
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 177 1453 1813 177
Size: 1276 360 2392
Fragments arranged by size:
2392 1276 360
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 1042 1042
Size: 4028
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 2051 2261 2051
Size: 210 3818
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SfcI C'TryA_G
Cuts at: 33 416 429 972 1071 1756 2749 2940 33
Size: 383 13 543 99 685 993 191 1121
Fragments arranged by size:
1121 993 685 543 383 191 99 13
SfoI GGC'GCC
Cuts at: 1460 1708 1460
Size: 248 3780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 1050 1050
Size: 4028
SmlI C'TyrA_G
Cuts at: 780 1183 2098 2590 2852 780
Size: 403 915 492 262 1956
Fragments arranged by size:
1956 915 492 403 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
StyI C'CwwG_G
Cuts at: 653 797 1162 1516 653
Size: 144 365 354 3165
Fragments arranged by size:
3165 365 354 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 291 1611 1744 2203 2412 291
Size: 1320 133 459 209 1907
Fragments arranged by size:
1907 1320 459 209 133
TatI w'GTAC_w
Cuts at: 206 701 3543 206
Size: 495 2842 691
Fragments arranged by size:
2842 691 495
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 3996
Tth111I GACn'n_nGTC
Cuts at: 1825 1825
Size: 4028
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1523 1523
Size: 4028
XmaI C'CCGG_G
Cuts at: 1048 1048
Size: 4028
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI