Restriction map for
pF1KB7397
(Circular) (Six-base) MAPSORT of: pF1KB7397.seq Check: 5879 from: 1 to: 3704
pF1KB7397 3704 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 301 742 301
Size: 441 3263
Acc65I G'GTAC_C
Cuts at: 720 720
Size: 3704
AclI AA'CG_TT
Cuts at: 3544 3544
Size: 3704
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1529 1961 2708 2976 1529
Size: 432 747 268 2257
Fragments arranged by size:
2257 747 432 268
AflIII A'CryG_T
Cuts at: 497 1056 2160 3137 497
Size: 559 1104 977 1064
Fragments arranged by size:
1104 1064 977 559
AgeI A'CCGG_T
Cuts at: 2092 2092
Size: 3704
AlwNI CAG_nnn'CTG
Cuts at: 540 684 1009 2576 540
Size: 144 325 1567 1668
Fragments arranged by size:
1668 1567 325 144
ApaLI G'TGCA_C
Cuts at: 2474 2474
Size: 3704
ApoI r'AATT_y
Cuts at: 225 708 3057 3313 225
Size: 483 2349 256 616
Fragments arranged by size:
2349 616 483 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3704
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
AvaI C'yCGr_G
Cuts at: 724 724
Size: 3704
BamHI G'GATC_C
Cuts at: 729 729
Size: 3704
BanI G'GyrC_C
Cuts at: 210 720 1134 1382 1417 210
Size: 510 414 248 35 2497
Fragments arranged by size:
2497 510 414 248 35
BanII G_rGCy'C
Cuts at: 718 1748 718
Size: 1030 2674
BbeI G_GCGC'C
Cuts at: 214 1138 1386 214
Size: 924 248 2532
Fragments arranged by size:
2532 924 248
BbsI GAAGACnn'nnnn_
Cuts at: 638 638
Size: 3704
BbvCI CC'TCA_GC
Cuts at: 471 471
Size: 3704
BciVI GTATCCnnnnn_n'
Cuts at: 1602 2363 3704 1602
Size: 761 1341 1602
Fragments arranged by size:
1602 1341 761
BclI T'GATC_A
Cuts at: 608 1224 608
Size: 616 3088
BglII A'GATC_T
Cuts at: 182 1219 3256 182
Size: 1037 2037 630
Fragments arranged by size:
2037 1037 630
BlpI GC'TnA_GC
Cuts at: 816 816
Size: 3704
Bme1580I G_kGCm'C
Cuts at: 1329 1422 2478 1329
Size: 93 1056 2555
Fragments arranged by size:
2555 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 916 997 1332 3476 916
Size: 81 335 2144 1144
Fragments arranged by size:
2144 1144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 3704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 562
Size: 3704
Bpu10I CC'TnA_GC
Cuts at: 471 471
Size: 3704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1759 2251 2549 880
Size: 879 492 298 2035
Fragments arranged by size:
2035 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 168 3408 168
Size: 3240 464
BsaAI yAC'GTr
Cuts at: 500 1057 500
Size: 557 3147
BsaBI GATnn'nnATC
Cuts at: 6 1218 3255 6
Size: 1212 2037 455
Fragments arranged by size:
2037 1212 455
BsaHI Gr'CG_yC
Cuts at: 211 1135 1383 2085 3581 211
Size: 924 248 702 1496 334
Fragments arranged by size:
1496 924 702 334 248
BsaWI w'CCGG_w
Cuts at: 121 893 1414 2092 2366 2513 2924 121
Size: 772 521 678 274 147 411 901
Fragments arranged by size:
901 772 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1129 1887 2464 2960 1129
Size: 758 577 496 1873
Fragments arranged by size:
1873 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 102
Size: 3704
BsiEI CG_ry'CG
Cuts at: 104 1246 1292 2500 104
Size: 1142 46 1208 1308
Fragments arranged by size:
1308 1208 1142 46
BsiHKAI G_wGCw'C
Cuts at: 718 1496 1686 2478 718
Size: 778 190 792 1944
Fragments arranged by size:
1944 792 778 190
BsiWI C'GTAC_G
Cuts at: 500 500
Size: 3704
BsmBI CGTCTCn'nnnn_
Cuts at: 510 510
Size: 3704
Bsp1286I G_dGCh'C
Cuts at: 718 1329 1422 1496 1686 1748 2478 718
Size: 611 93 74 190 62 730 1944
Fragments arranged by size:
1944 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2924 2924
Size: 3704
BspHI T'CATG_A
Cuts at: 2880 2880
Size: 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 754 968 1270 1651 754
Size: 214 302 381 2807
Fragments arranged by size:
2807 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1996 2050 3534 3699 50
Size: 1946 54 1484 165 55
Fragments arranged by size:
1946 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1616 1616
Size: 3704
BsrFI r'CCGG_y
Cuts at: 1702 1883 2092 1702
Size: 181 209 3314
Fragments arranged by size:
3314 209 181
BsrGI T'GTAC_A
Cuts at: 411 411
Size: 3704
BssHII G'CGCG_C
Cuts at: 149 149
Size: 3704
BssSI C'ACGA_G
Cuts at: 1975 2333 1975
Size: 358 3346
BstAPI GCAn_nnn'nTGC
Cuts at: 1081 1081
Size: 3704
BstBI TT'CG_AA
Cuts at: 2065 2065
Size: 3704
BstEII G'GTnAC_C
Cuts at: 324 324
Size: 3704
BstYI r'GATC_y
Cuts at: 1 182 729 1196 1219 1554 1800 2812 2898
Size: 181 547 467 23 335 246 1012 86
Cuts at: 2898 2910 3256 1
Size: 12 346 449
Fragments arranged by size:
1012 547 467 449 346 335 246 181
86 23 12
BstZ17I GTA'TAC
Cuts at: 302 302
Size: 3704
BtgI C'CryG_G
Cuts at: 107 477 620 107
Size: 370 143 3191
Fragments arranged by size:
3191 370 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1654 1835 1998 1654
Size: 181 163 3360
Fragments arranged by size:
3360 181 163
BtsI GCAGTG_nn'
Cuts at: 947 947
Size: 3704
DraI TTT'AAA
Cuts at: 703 703
Size: 3704
DrdI GACnn_nn'nnGTC
Cuts at: 1410 2268 1410
Size: 858 2846
EaeI y'GGCC_r
Cuts at: 1289 1463 1854 1881 3226 1289
Size: 174 391 27 1345 1767
Fragments arranged by size:
1767 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1289 1289
Size: 3704
EarI CTCTTCn'nnn_
Cuts at: 450 1727 1937 450
Size: 1277 210 2217
Fragments arranged by size:
2217 1277 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1912 2222 2368 3514 1912
Size: 310 146 1146 2102
Fragments arranged by size:
2102 1146 310 146
EcoICRI GAG'CTC
Cuts at: 716 716
Size: 3704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1529 1961 2708 2976 562
Size: 967 432 747 268 1290
Fragments arranged by size:
1290 967 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 843 843
Size: 3704
EcoRI G'AATT_C
Cuts at: 708 708
Size: 3704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1723 1723
Size: 3704
FspI TGC'GCA
Cuts at: 1485 1485
Size: 3704
HaeII r_GCGC'y
Cuts at: 214 1138 1386 2408 3387 214
Size: 924 248 1022 979 531
Fragments arranged by size:
1022 979 924 531 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1546 1576 1578 1608 1546
Size: 30 2 30 3642
Fragments arranged by size:
3642 30 30 2
HincII GTy'rAC
Cuts at: 528 743 528
Size: 215 3489
KasI G'GCGC_C
Cuts at: 210 1134 1382 210
Size: 924 248 2532
Fragments arranged by size:
2532 924 248
KpnI G_GTAC'C
Cuts at: 724 724
Size: 3704
MluI A'CGCG_T
Cuts at: 3137 3137
Size: 3704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2375 2559 2375
Size: 184 3520
MscI TGG'CCA
Cuts at: 1465 1465
Size: 3704
MslI CAynn'nnrTG
Cuts at: 295 642 1693 1820 295
Size: 347 1051 127 2179
Fragments arranged by size:
2179 1051 347 127
MspA1I CmG'CkG
Cuts at: 815 1129 1489 2502 2747 815
Size: 314 360 1013 245 1772
Fragments arranged by size:
1772 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1885 1885
Size: 3704
NarI GG'CG_CC
Cuts at: 211 1135 1383 211
Size: 924 248 2532
Fragments arranged by size:
2532 924 248
NcoI C'CATG_G
Cuts at: 107 477 620 107
Size: 370 143 3191
Fragments arranged by size:
3191 370 143
NgoMIV G'CCGG_C
Cuts at: 1883 1883
Size: 3704
NspI r_CATG'y
Cuts at: 757 1257 2164 757
Size: 500 907 2297
Fragments arranged by size:
2297 907 500
PciI A'CATG_T
Cuts at: 2160 2160
Size: 3704
PflMI CCAn_nnn'nTGG
Cuts at: 540 540
Size: 3704
PmeI GTTT'AAAC
Cuts at: 703 703
Size: 3704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2843 2875 2843
Size: 32 3672
PstI C_TGCA'G
Cuts at: 158 751 1436 158
Size: 593 685 2426
Fragments arranged by size:
2426 685 593
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3704
PvuII CAG'CTG
Cuts at: 1129 1489 1129
Size: 360 3344
RsrII CG'GwC_CG
Cuts at: 1899 1899
Size: 3704
SacI G_AGCT'C
Cuts at: 718 718
Size: 3704
SalI G'TCGA_C
Cuts at: 741 741
Size: 3704
SapI GCTCTTCn'nnn_
Cuts at: 1727 1937 1727
Size: 210 3494
SbfI CC_TGCA'GG
Cuts at: 751 751
Size: 3704
ScaI AGT'ACT
Cuts at: 3221 3221
Size: 3704
SfcI C'TryA_G
Cuts at: 33 154 747 1432 2425 2616 33
Size: 121 593 685 993 191 1121
Fragments arranged by size:
1121 993 685 593 191 121
SfoI GGC'GCC
Cuts at: 212 1136 1384 212
Size: 924 248 2532
Fragments arranged by size:
2532 924 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
SmaI CCC'GGG
Cuts at: 726 726
Size: 3704
SmlI C'TyrA_G
Cuts at: 859 1774 2266 2528 859
Size: 915 492 262 2035
Fragments arranged by size:
2035 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3704
SphI G_CATG'C
Cuts at: 757 1257 757
Size: 500 3204
StyI C'CwwG_G
Cuts at: 107 195 477 620 838 1192 107
Size: 88 282 143 218 354 2619
Fragments arranged by size:
2619 354 282 218 143 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 407 1287 1420 1879 2088 407
Size: 880 133 459 209 2023
Fragments arranged by size:
2023 880 459 209 133
TatI w'GTAC_w
Cuts at: 275 411 3219 275
Size: 136 2808 760
Fragments arranged by size:
2808 760 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 3672
Tth111I GACn'n_nGTC
Cuts at: 1501 1501
Size: 3704
XbaI T'CTAG_A
Cuts at: 735 735
Size: 3704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1199 1199
Size: 3704
XmaI C'CCGG_G
Cuts at: 724 724
Size: 3704
XmnI GAAnn'nnTTC
Cuts at: 337 337
Size: 3704
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsaXI BseRI BsmI BstXI Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI