Restriction map for
pF1KB7395
(Circular) (Six-base) MAPSORT of: pF1KB7395.seq Check: 6761 from: 1 to: 3776
pF1KB7395 3776 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 814 814
Size: 3776
Acc65I G'GTAC_C
Cuts at: 401 792 401
Size: 391 3385
AclI AA'CG_TT
Cuts at: 3616 3616
Size: 3776
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1601 2033 2780 3048 1601
Size: 432 747 268 2329
Fragments arranged by size:
2329 747 432 268
AflIII A'CryG_T
Cuts at: 1128 2232 3209 1128
Size: 1104 977 1695
Fragments arranged by size:
1695 1104 977
AgeI A'CCGG_T
Cuts at: 2164 2164
Size: 3776
AlwNI CAG_nnn'CTG
Cuts at: 1081 2648 1081
Size: 1567 2209
ApaLI G'TGCA_C
Cuts at: 2546 2546
Size: 3776
ApoI r'AATT_y
Cuts at: 780 3129 3385 780
Size: 2349 256 1171
Fragments arranged by size:
2349 1171 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3776
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
AvaI C'yCGr_G
Cuts at: 649 796 649
Size: 147 3629
BamHI G'GATC_C
Cuts at: 801 801
Size: 3776
BanI G'GyrC_C
Cuts at: 401 792 1206 1454 1489 401
Size: 391 414 248 35 2688
Fragments arranged by size:
2688 414 391 248 35
BanII G_rGCy'C
Cuts at: 790 1820 790
Size: 1030 2746
BbeI G_GCGC'C
Cuts at: 1210 1458 1210
Size: 248 3528
BciVI GTATCCnnnnn_n'
Cuts at: 1674 2435 3776 1674
Size: 761 1341 1674
Fragments arranged by size:
1674 1341 761
BclI T'GATC_A
Cuts at: 501 1296 501
Size: 795 2981
BglII A'GATC_T
Cuts at: 1291 3328 1291
Size: 2037 1739
BlpI GC'TnA_GC
Cuts at: 733 888 733
Size: 155 3621
Bme1580I G_kGCm'C
Cuts at: 1401 1494 2550 1401
Size: 93 1056 2627
Fragments arranged by size:
2627 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 988 1069 1404 3548 988
Size: 81 335 2144 1216
Fragments arranged by size:
2144 1216 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 3776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 952 1831 2323 2621 158
Size: 794 879 492 298 1313
Fragments arranged by size:
1313 879 794 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3480 3480
Size: 3776
BsaAI yAC'GTr
Cuts at: 1129 1129
Size: 3776
BsaBI GATnn'nnATC
Cuts at: 6 1290 3327 6
Size: 1284 2037 455
Fragments arranged by size:
2037 1284 455
BsaHI Gr'CG_yC
Cuts at: 1207 1455 2157 3653 1207
Size: 248 702 1496 1330
Fragments arranged by size:
1496 1330 702 248
BsaWI w'CCGG_w
Cuts at: 965 1486 2164 2438 2585 2996 965
Size: 521 678 274 147 411 1745
Fragments arranged by size:
1745 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 174 649 174
Size: 475 3301
BseYI C'CCAG_C
Cuts at: 1201 1959 2536 3032 1201
Size: 758 577 496 1945
Fragments arranged by size:
1945 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1318 1364 2572 104
Size: 1214 46 1208 1308
Fragments arranged by size:
1308 1214 1208 46
BsiHKAI G_wGCw'C
Cuts at: 739 790 1568 1758 2550 739
Size: 51 778 190 792 1965
Fragments arranged by size:
1965 792 778 190 51
Bsp1286I G_dGCh'C
Cuts at: 739 790 1401 1494 1568 1758 1820 2550 739
Size: 51 611 93 74 190 62 730 1965
Fragments arranged by size:
1965 730 611 190 93 74 62 51
BspEI T'CCGG_A
Cuts at: 2996 2996
Size: 3776
BspHI T'CATG_A
Cuts at: 2952 2952
Size: 3776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 826 1040 1342 1723 826
Size: 214 302 381 2879
Fragments arranged by size:
2879 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2068 2122 3606 3771 50
Size: 2018 54 1484 165 55
Fragments arranged by size:
2018 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 132 1688 132
Size: 1556 2220
BsrFI r'CCGG_y
Cuts at: 1774 1955 2164 1774
Size: 181 209 3386
Fragments arranged by size:
3386 209 181
BssSI C'ACGA_G
Cuts at: 2047 2405 2047
Size: 358 3418
BstAPI GCAn_nnn'nTGC
Cuts at: 1153 1153
Size: 3776
BstBI TT'CG_AA
Cuts at: 2137 2137
Size: 3776
BstYI r'GATC_y
Cuts at: 1 801 1268 1291 1626 1872 2884 2970 2982
Size: 800 467 23 335 246 1012 86 12
Cuts at: 2982 3328 1
Size: 346 449
Fragments arranged by size:
1012 800 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1726 1907 2070 1726
Size: 181 163 3432
Fragments arranged by size:
3432 181 163
BtsI GCAGTG_nn'
Cuts at: 718 1019 718
Size: 301 3475
DraI TTT'AAA
Cuts at: 576 775 576
Size: 199 3577
DrdI GACnn_nn'nnGTC
Cuts at: 1482 2340 1482
Size: 858 2918
EaeI y'GGCC_r
Cuts at: 1361 1535 1926 1953 3298 1361
Size: 174 391 27 1345 1839
Fragments arranged by size:
1839 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1361 1361
Size: 3776
EarI CTCTTCn'nnn_
Cuts at: 518 650 1799 2009 518
Size: 132 1149 210 2285
Fragments arranged by size:
2285 1149 210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1984 2294 2440 3586 1984
Size: 310 146 1146 2174
Fragments arranged by size:
2174 1146 310 146
EcoICRI GAG'CTC
Cuts at: 788 788
Size: 3776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1601 2033 2780 3048 1601
Size: 432 747 268 2329
Fragments arranged by size:
2329 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 915 915
Size: 3776
EcoRI G'AATT_C
Cuts at: 780 780
Size: 3776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1795 1795
Size: 3776
FspI TGC'GCA
Cuts at: 1557 1557
Size: 3776
HaeII r_GCGC'y
Cuts at: 1210 1458 2480 3459 1210
Size: 248 1022 979 1527
Fragments arranged by size:
1527 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1618 1648 1650 1680 1618
Size: 30 2 30 3714
Fragments arranged by size:
3714 30 30 2
HincII GTy'rAC
Cuts at: 815 815
Size: 3776
KasI G'GCGC_C
Cuts at: 1206 1454 1206
Size: 248 3528
KpnI G_GTAC'C
Cuts at: 405 796 405
Size: 391 3385
MluI A'CGCG_T
Cuts at: 3209 3209
Size: 3776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 222 2447 2631 222
Size: 2225 184 1367
Fragments arranged by size:
2225 1367 184
MscI TGG'CCA
Cuts at: 1537 1537
Size: 3776
MslI CAynn'nnrTG
Cuts at: 1765 1892 1765
Size: 127 3649
MspA1I CmG'CkG
Cuts at: 732 887 1201 1561 2574 2819 732
Size: 155 314 360 1013 245 1689
Fragments arranged by size:
1689 1013 360 314 245 155
NaeI GCC'GGC
Cuts at: 1957 1957
Size: 3776
NarI GG'CG_CC
Cuts at: 1207 1455 1207
Size: 248 3528
NgoMIV G'CCGG_C
Cuts at: 1955 1955
Size: 3776
NspI r_CATG'y
Cuts at: 829 1329 2236 829
Size: 500 907 2369
Fragments arranged by size:
2369 907 500
PciI A'CATG_T
Cuts at: 2232 2232
Size: 3776
PmeI GTTT'AAAC
Cuts at: 775 775
Size: 3776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2915 2947 2915
Size: 32 3744
PsiI TTA'TAA
Cuts at: 695 695
Size: 3776
PspXI vC'TCGA_Gb
Cuts at: 649 649
Size: 3776
PstI C_TGCA'G
Cuts at: 416 599 725 823 1508 416
Size: 183 126 98 685 2684
Fragments arranged by size:
2684 685 183 126 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3776
PvuII CAG'CTG
Cuts at: 732 1201 1561 732
Size: 469 360 2947
Fragments arranged by size:
2947 469 360
RsrII CG'GwC_CG
Cuts at: 1971 1971
Size: 3776
SacI G_AGCT'C
Cuts at: 790 790
Size: 3776
SalI G'TCGA_C
Cuts at: 813 813
Size: 3776
SapI GCTCTTCn'nnn_
Cuts at: 1799 2009 1799
Size: 210 3566
SbfI CC_TGCA'GG
Cuts at: 823 823
Size: 3776
ScaI AGT'ACT
Cuts at: 3293 3293
Size: 3776
SfcI C'TryA_G
Cuts at: 33 412 595 721 819 1504 2497 2688 33
Size: 379 183 126 98 685 993 191 1121
Fragments arranged by size:
1121 993 685 379 191 183 126 98
SfoI GGC'GCC
Cuts at: 1208 1456 1208
Size: 248 3528
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
SmaI CCC'GGG
Cuts at: 798 798
Size: 3776
SmlI C'TyrA_G
Cuts at: 173 649 931 1846 2338 2600 173
Size: 476 282 915 492 262 1349
Fragments arranged by size:
1349 915 492 476 282 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3776
SphI G_CATG'C
Cuts at: 829 1329 829
Size: 500 3276
StyI C'CwwG_G
Cuts at: 274 910 1264 274
Size: 636 354 2786
Fragments arranged by size:
2786 636 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1359 1492 1951 2160 1359
Size: 133 459 209 2975
Fragments arranged by size:
2975 459 209 133
TatI w'GTAC_w
Cuts at: 223 3291 223
Size: 3068 708
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 3744
Tth111I GACn'n_nGTC
Cuts at: 1573 1573
Size: 3776
XbaI T'CTAG_A
Cuts at: 807 807
Size: 3776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1271 1271
Size: 3776
XhoI C'TCGA_G
Cuts at: 649 649
Size: 3776
XmaI C'CCGG_G
Cuts at: 796 796
Size: 3776
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PsiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI ZraI