Restriction map for
pF1KB7394
(Circular) (Six-base) MAPSORT of: pF1KB7394.seq Check: 1126 from: 1 to: 3725
pF1KB7394 3725 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 763 763
Size: 3725
Acc65I G'GTAC_C
Cuts at: 741 741
Size: 3725
AclI AA'CG_TT
Cuts at: 3565 3565
Size: 3725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 1550 1982 2729 2997 375
Size: 1175 432 747 268 1103
Fragments arranged by size:
1175 1103 747 432 268
AfeI AGC'GCT
Cuts at: 208 208
Size: 3725
AflIII A'CryG_T
Cuts at: 1077 2181 3158 1077
Size: 1104 977 1644
Fragments arranged by size:
1644 1104 977
AgeI A'CCGG_T
Cuts at: 2113 2113
Size: 3725
AlwNI CAG_nnn'CTG
Cuts at: 1030 2597 1030
Size: 1567 2158
ApaLI G'TGCA_C
Cuts at: 2495 2495
Size: 3725
ApoI r'AATT_y
Cuts at: 712 729 3078 3334 712
Size: 17 2349 256 1103
Fragments arranged by size:
2349 1103 256 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 3725
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
AvaI C'yCGr_G
Cuts at: 745 745
Size: 3725
BamHI G'GATC_C
Cuts at: 750 750
Size: 3725
BanI G'GyrC_C
Cuts at: 182 539 557 741 1155 1403 1438 182
Size: 357 18 184 414 248 35 2469
Fragments arranged by size:
2469 414 357 248 184 35 18
BanII G_rGCy'C
Cuts at: 166 288 497 739 1769 166
Size: 122 209 242 1030 2122
Fragments arranged by size:
2122 1030 242 209 122
BbeI G_GCGC'C
Cuts at: 1159 1407 1159
Size: 248 3477
BbsI GAAGACnn'nnnn_
Cuts at: 457 457
Size: 3725
BciVI GTATCCnnnnn_n'
Cuts at: 1623 2384 3725 1623
Size: 761 1341 1623
Fragments arranged by size:
1623 1341 761
BclI T'GATC_A
Cuts at: 1245 1245
Size: 3725
BglII A'GATC_T
Cuts at: 1240 3277 1240
Size: 2037 1688
BlpI GC'TnA_GC
Cuts at: 599 837 599
Size: 238 3487
Bme1580I G_kGCm'C
Cuts at: 481 1350 1443 2499 481
Size: 869 93 1056 1707
Fragments arranged by size:
1707 1056 869 93
BmgBI CAC'GTC
Cuts at: 627 627
Size: 3725
BmrI ACTGGGnnnn_n'
Cuts at: 470 694 937 1018 1353 3497 470
Size: 224 243 81 335 2144 698
Fragments arranged by size:
2144 698 335 243 224 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 3725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 1780 2272 2570 901
Size: 879 492 298 2056
Fragments arranged by size:
2056 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 460 3429 460
Size: 2969 756
BsaAI yAC'GTr
Cuts at: 1078 1078
Size: 3725
BsaBI GATnn'nnATC
Cuts at: 6 1239 3276 6
Size: 1233 2037 455
Fragments arranged by size:
2037 1233 455
BsaHI Gr'CG_yC
Cuts at: 1156 1404 2106 3602 1156
Size: 248 702 1496 1279
Fragments arranged by size:
1496 1279 702 248
BsaWI w'CCGG_w
Cuts at: 914 1435 2113 2387 2534 2945 914
Size: 521 678 274 147 411 1694
Fragments arranged by size:
1694 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 362 362
Size: 3725
BseYI C'CCAG_C
Cuts at: 352 382 586 1150 1908 2485 2981 352
Size: 30 204 564 758 577 496 1096
Fragments arranged by size:
1096 758 577 564 496 204 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 348
Size: 3725
BsiEI CG_ry'CG
Cuts at: 104 547 1267 1313 2521 104
Size: 443 720 46 1208 1308
Fragments arranged by size:
1308 1208 720 443 46
BsiHKAI G_wGCw'C
Cuts at: 166 248 739 1517 1707 2499 166
Size: 82 491 778 190 792 1392
Fragments arranged by size:
1392 792 778 491 190 82
Bsp1286I G_dGCh'C
Cuts at: 166 248 288 481 497 739 1350 1443 1517
Size: 82 40 193 16 242 611 93 74
Cuts at: 1517 1707 1769 2499 166
Size: 190 62 730 1392
Fragments arranged by size:
1392 730 611 242 193 190 93 82
74 62 40 16
BspEI T'CCGG_A
Cuts at: 2945 2945
Size: 3725
BspHI T'CATG_A
Cuts at: 2901 2901
Size: 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 775 989 1291 1672 775
Size: 214 302 381 2828
Fragments arranged by size:
2828 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2017 2071 3555 3720 50
Size: 1967 54 1484 165 55
Fragments arranged by size:
1967 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1637 1637
Size: 3725
BsrFI r'CCGG_y
Cuts at: 1723 1904 2113 1723
Size: 181 209 3335
Fragments arranged by size:
3335 209 181
BsrGI T'GTAC_A
Cuts at: 335 335
Size: 3725
BssSI C'ACGA_G
Cuts at: 1996 2354 1996
Size: 358 3367
BstAPI GCAn_nnn'nTGC
Cuts at: 1102 1102
Size: 3725
BstBI TT'CG_AA
Cuts at: 2086 2086
Size: 3725
BstYI r'GATC_y
Cuts at: 1 750 1217 1240 1575 1821 2833 2919 2931
Size: 749 467 23 335 246 1012 86 12
Cuts at: 2931 3277 1
Size: 346 449
Fragments arranged by size:
1012 749 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 689 1675 1856 2019 689
Size: 986 181 163 2395
Fragments arranged by size:
2395 986 181 163
BtsI GCAGTG_nn'
Cuts at: 968 968
Size: 3725
DraI TTT'AAA
Cuts at: 724 724
Size: 3725
DrdI GACnn_nn'nnGTC
Cuts at: 1431 2289 1431
Size: 858 2867
EaeI y'GGCC_r
Cuts at: 1310 1484 1875 1902 3247 1310
Size: 174 391 27 1345 1788
Fragments arranged by size:
1788 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1310 1310
Size: 3725
EarI CTCTTCn'nnn_
Cuts at: 1748 1958 1748
Size: 210 3515
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1933 2243 2389 3535 1933
Size: 310 146 1146 2123
Fragments arranged by size:
2123 1146 310 146
EcoICRI GAG'CTC
Cuts at: 164 737 164
Size: 573 3152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 1550 1982 2729 2997 375
Size: 1175 432 747 268 1103
Fragments arranged by size:
1175 1103 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 864 864
Size: 3725
EcoRI G'AATT_C
Cuts at: 729 729
Size: 3725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1744 1744
Size: 3725
FspI TGC'GCA
Cuts at: 1506 1506
Size: 3725
HaeII r_GCGC'y
Cuts at: 210 1159 1407 2429 3408 210
Size: 949 248 1022 979 527
Fragments arranged by size:
1022 979 949 527 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1567 1597 1599 1629 1567
Size: 30 2 30 3663
Fragments arranged by size:
3663 30 30 2
HincII GTy'rAC
Cuts at: 764 764
Size: 3725
KasI G'GCGC_C
Cuts at: 1155 1403 1155
Size: 248 3477
KpnI G_GTAC'C
Cuts at: 745 745
Size: 3725
MluI A'CGCG_T
Cuts at: 3158 3158
Size: 3725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2396 2580 2396
Size: 184 3541
MscI TGG'CCA
Cuts at: 1486 1486
Size: 3725
MslI CAynn'nnrTG
Cuts at: 1714 1841 1714
Size: 127 3598
MspA1I CmG'CkG
Cuts at: 836 1150 1510 2523 2768 836
Size: 314 360 1013 245 1793
Fragments arranged by size:
1793 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1906 1906
Size: 3725
NarI GG'CG_CC
Cuts at: 1156 1404 1156
Size: 248 3477
NdeI CA'TA_TG
Cuts at: 446 446
Size: 3725
NgoMIV G'CCGG_C
Cuts at: 1904 1904
Size: 3725
NspI r_CATG'y
Cuts at: 241 778 1278 2185 241
Size: 537 500 907 1781
Fragments arranged by size:
1781 907 537 500
PasI CC'CwG_GG
Cuts at: 168 299 168
Size: 131 3594
PciI A'CATG_T
Cuts at: 2181 2181
Size: 3725
PflMI CCAn_nnn'nTGG
Cuts at: 113 280 412 113
Size: 167 132 3426
Fragments arranged by size:
3426 167 132
PmeI GTTT'AAAC
Cuts at: 724 724
Size: 3725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2864 2896 2864
Size: 32 3693
PstI C_TGCA'G
Cuts at: 772 1457 772
Size: 685 3040
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3725
PvuII CAG'CTG
Cuts at: 1150 1510 1150
Size: 360 3365
RsrII CG'GwC_CG
Cuts at: 1920 1920
Size: 3725
SacI G_AGCT'C
Cuts at: 166 739 166
Size: 573 3152
SalI G'TCGA_C
Cuts at: 762 762
Size: 3725
SapI GCTCTTCn'nnn_
Cuts at: 1748 1958 1748
Size: 210 3515
SbfI CC_TGCA'GG
Cuts at: 772 772
Size: 3725
ScaI AGT'ACT
Cuts at: 3242 3242
Size: 3725
SfcI C'TryA_G
Cuts at: 33 768 1453 2446 2637 33
Size: 735 685 993 191 1121
Fragments arranged by size:
1121 993 735 685 191
SfoI GGC'GCC
Cuts at: 1157 1405 1157
Size: 248 3477
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
SmaI CCC'GGG
Cuts at: 747 747
Size: 3725
SmlI C'TyrA_G
Cuts at: 880 1795 2287 2549 880
Size: 915 492 262 2056
Fragments arranged by size:
2056 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3725
SphI G_CATG'C
Cuts at: 241 778 1278 241
Size: 537 500 2688
Fragments arranged by size:
2688 537 500
StyI C'CwwG_G
Cuts at: 406 423 859 1213 406
Size: 17 436 354 2918
Fragments arranged by size:
2918 436 354 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1308 1441 1900 2109 1308
Size: 133 459 209 2924
Fragments arranged by size:
2924 459 209 133
TatI w'GTAC_w
Cuts at: 335 3240 335
Size: 2905 820
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 3693
Tth111I GACn'n_nGTC
Cuts at: 548 1522 548
Size: 974 2751
XbaI T'CTAG_A
Cuts at: 756 756
Size: 3725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1220 1220
Size: 3725
XmaI C'CCGG_G
Cuts at: 745 745
Size: 3725
XmnI GAAnn'nnTTC
Cuts at: 454 454
Size: 3725
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NheI
NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI