Restriction map for
pF1KB7392
(Circular) (Six-base) MAPSORT of: pF1KB7392.seq Check: 3501 from: 1 to: 4133
pF1KB7392 4133 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AatII G_ACGT'C
Cuts at: 247 247
Size: 4133
AccI GT'mk_AC
Cuts at: 1171 1171
Size: 4133
Acc65I G'GTAC_C
Cuts at: 1149 1149
Size: 4133
AclI AA'CG_TT
Cuts at: 3973 3973
Size: 4133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1958 2390 3137 3405 1958
Size: 432 747 268 2686
Fragments arranged by size:
2686 747 432 268
AflIII A'CryG_T
Cuts at: 1485 2589 3566 1485
Size: 1104 977 2052
Fragments arranged by size:
2052 1104 977
AgeI A'CCGG_T
Cuts at: 2521 2521
Size: 4133
AlwNI CAG_nnn'CTG
Cuts at: 1438 3005 1438
Size: 1567 2566
ApaLI G'TGCA_C
Cuts at: 2903 2903
Size: 4133
ApoI r'AATT_y
Cuts at: 146 652 1137 3486 3742 146
Size: 506 485 2349 256 537
Fragments arranged by size:
2349 537 506 485 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4133
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
AvaI C'yCGr_G
Cuts at: 283 416 878 1153 283
Size: 133 462 275 3263
Fragments arranged by size:
3263 462 275 133
AvrII C'CTAG_G
Cuts at: 912 912
Size: 4133
BamHI G'GATC_C
Cuts at: 1158 1158
Size: 4133
BanI G'GyrC_C
Cuts at: 966 1149 1563 1811 1846 966
Size: 183 414 248 35 3253
Fragments arranged by size:
3253 414 248 183 35
BanII G_rGCy'C
Cuts at: 219 398 812 1017 1147 2177 219
Size: 179 414 205 130 1030 2175
Fragments arranged by size:
2175 1030 414 205 179 130
BbeI G_GCGC'C
Cuts at: 970 1567 1815 970
Size: 597 248 3288
Fragments arranged by size:
3288 597 248
BbsI GAAGACnn'nnnn_
Cuts at: 350 989 350
Size: 639 3494
BbvCI CC'TCA_GC
Cuts at: 977 977
Size: 4133
BciVI GTATCCnnnnn_n'
Cuts at: 2031 2792 4133 2031
Size: 761 1341 2031
Fragments arranged by size:
2031 1341 761
BclI T'GATC_A
Cuts at: 203 1653 203
Size: 1450 2683
BglII A'GATC_T
Cuts at: 1648 3685 1648
Size: 2037 2096
BlpI GC'TnA_GC
Cuts at: 1245 1245
Size: 4133
Bme1580I G_kGCm'C
Cuts at: 1758 1851 2907 1758
Size: 93 1056 2984
Fragments arranged by size:
2984 1056 93
BmgBI CAC'GTC
Cuts at: 201 201
Size: 4133
BmrI ACTGGGnnnn_n'
Cuts at: 1345 1426 1761 3905 1345
Size: 81 335 2144 1573
Fragments arranged by size:
2144 1573 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 4133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1063
Size: 4133
Bpu10I CC'TnA_GC
Cuts at: 977 977
Size: 4133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 1309 2188 2680 2978 391
Size: 918 879 492 298 1546
Fragments arranged by size:
1546 918 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3837 3837
Size: 4133
BsaAI yAC'GTr
Cuts at: 1486 1486
Size: 4133
BsaBI GATnn'nnATC
Cuts at: 6 1647 3684 6
Size: 1641 2037 455
Fragments arranged by size:
2037 1641 455
BsaHI Gr'CG_yC
Cuts at: 244 967 1564 1812 2514 4010 244
Size: 723 597 248 702 1496 367
Fragments arranged by size:
1496 723 702 597 367 248
BsaWI w'CCGG_w
Cuts at: 768 1322 1843 2521 2795 2942 3353 768
Size: 554 521 678 274 147 411 1548
Fragments arranged by size:
1548 678 554 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1061 1091 1061
Size: 30 4103
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 316 325 328 331 334 406 316
Size: 9 3 3 3 72 4043
Fragments arranged by size:
4043 72 9 3 3 3
BseYI C'CCAG_C
Cuts at: 1558 2316 2893 3389 1558
Size: 758 577 496 2302
Fragments arranged by size:
2302 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 905 905
Size: 4133
BsiEI CG_ry'CG
Cuts at: 104 1675 1721 2929 104
Size: 1571 46 1208 1308
Fragments arranged by size:
1571 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 398 1147 1925 2115 2907 398
Size: 749 778 190 792 1624
Fragments arranged by size:
1624 792 778 749 190
Bsp1286I G_dGCh'C
Cuts at: 219 398 812 1017 1147 1758 1851 1925 2115
Size: 179 414 205 130 611 93 74 190
Cuts at: 2115 2177 2907 219
Size: 62 730 1445
Fragments arranged by size:
1445 730 611 414 205 190 179 130
93 74 62
BspEI T'CCGG_A
Cuts at: 768 3353 768
Size: 2585 1548
BspHI T'CATG_A
Cuts at: 3309 3309
Size: 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1183 1397 1699 2080 1183
Size: 214 302 381 3236
Fragments arranged by size:
3236 381 302 214
BsrBI CCG'CTC
Cuts at: 50 423 2425 2479 3963 4128 50
Size: 373 2002 54 1484 165 55
Fragments arranged by size:
2002 1484 373 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2045 2045
Size: 4133
BsrFI r'CCGG_y
Cuts at: 1055 2131 2312 2521 1055
Size: 1076 181 209 2667
Fragments arranged by size:
2667 1076 209 181
BssSI C'ACGA_G
Cuts at: 388 2404 2762 388
Size: 2016 358 1759
Fragments arranged by size:
2016 1759 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1510 1510
Size: 4133
BstBI TT'CG_AA
Cuts at: 2494 2494
Size: 4133
BstYI r'GATC_y
Cuts at: 1 916 1158 1625 1648 1983 2229 3241 3327
Size: 915 242 467 23 335 246 1012 86
Cuts at: 3327 3339 3685 1
Size: 12 346 449
Fragments arranged by size:
1012 915 467 449 346 335 246 242
86 23 12
BtgI C'CryG_G
Cuts at: 703 703
Size: 4133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2083 2264 2427 2083
Size: 181 163 3789
Fragments arranged by size:
3789 181 163
BtsI GCAGTG_nn'
Cuts at: 1376 1376
Size: 4133
DraI TTT'AAA
Cuts at: 1132 1132
Size: 4133
DrdI GACnn_nn'nnGTC
Cuts at: 1839 2697 1839
Size: 858 3275
EaeI y'GGCC_r
Cuts at: 706 1718 1892 2283 2310 3655 706
Size: 1012 174 391 27 1345 1184
Fragments arranged by size:
1345 1184 1012 391 174 27
EagI C'GGCC_G
Cuts at: 1718 1718
Size: 4133
EarI CTCTTCn'nnn_
Cuts at: 205 293 302 320 326 819 1045 2156 2366
Size: 88 9 18 6 493 226 1111 210
Cuts at: 2366 205
Size: 1972
Fragments arranged by size:
1972 1111 493 226 210 88 18 9
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2341 2651 2797 3943 2341
Size: 310 146 1146 2531
Fragments arranged by size:
2531 1146 310 146
EcoICRI GAG'CTC
Cuts at: 396 1145 396
Size: 749 3384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1958 2390 3137 3405 1063
Size: 895 432 747 268 1791
Fragments arranged by size:
1791 895 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1272 1272
Size: 4133
EcoRI G'AATT_C
Cuts at: 146 1137 146
Size: 991 3142
EcoRV GAT'ATC
Cuts at: 175 175
Size: 4133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 166 2152 166
Size: 1986 2147
FspI TGC'GCA
Cuts at: 1914 1914
Size: 4133
HaeII r_GCGC'y
Cuts at: 970 977 1567 1815 2837 3816 970
Size: 7 590 248 1022 979 1287
Fragments arranged by size:
1287 1022 979 590 248 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 586 618 874 906 1108 1140 1975 2005 2007
Size: 32 256 32 202 32 835 30 2
Cuts at: 2007 2037 586
Size: 30 2682
Fragments arranged by size:
2682 835 256 202 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1172 1172
Size: 4133
KasI G'GCGC_C
Cuts at: 966 1563 1811 966
Size: 597 248 3288
Fragments arranged by size:
3288 597 248
KpnI G_GTAC'C
Cuts at: 1153 1153
Size: 4133
MluI A'CGCG_T
Cuts at: 3566 3566
Size: 4133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 690 2804 2988 690
Size: 2114 184 1835
Fragments arranged by size:
2114 1835 184
MscI TGG'CCA
Cuts at: 1894 1894
Size: 4133
MslI CAynn'nnrTG
Cuts at: 529 2122 2249 529
Size: 1593 127 2413
Fragments arranged by size:
2413 1593 127
MspA1I CmG'CkG
Cuts at: 195 744 1244 1558 1918 2931 3176 195
Size: 549 500 314 360 1013 245 1152
Fragments arranged by size:
1152 1013 549 500 360 314 245
NaeI GCC'GGC
Cuts at: 2314 2314
Size: 4133
NarI GG'CG_CC
Cuts at: 967 1564 1812 967
Size: 597 248 3288
Fragments arranged by size:
3288 597 248
NgoMIV G'CCGG_C
Cuts at: 2312 2312
Size: 4133
NspI r_CATG'y
Cuts at: 1186 1686 2593 1186
Size: 500 907 2726
Fragments arranged by size:
2726 907 500
PciI A'CATG_T
Cuts at: 2589 2589
Size: 4133
PflMI CCAn_nnn'nTGG
Cuts at: 130 354 130
Size: 224 3909
PmeI GTTT'AAAC
Cuts at: 1132 1132
Size: 4133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 4101
PstI C_TGCA'G
Cuts at: 1180 1865 1180
Size: 685 3448
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4133
PvuII CAG'CTG
Cuts at: 1558 1918 1558
Size: 360 3773
RsrII CG'GwC_CG
Cuts at: 2328 2328
Size: 4133
SacI G_AGCT'C
Cuts at: 398 1147 398
Size: 749 3384
SalI G'TCGA_C
Cuts at: 1170 1170
Size: 4133
SapI GCTCTTCn'nnn_
Cuts at: 1045 2156 2366 1045
Size: 1111 210 2812
Fragments arranged by size:
2812 1111 210
SbfI CC_TGCA'GG
Cuts at: 1180 1180
Size: 4133
ScaI AGT'ACT
Cuts at: 723 3650 723
Size: 2927 1206
SfcI C'TryA_G
Cuts at: 33 1176 1861 2854 3045 33
Size: 1143 685 993 191 1121
Fragments arranged by size:
1143 1121 993 685 191
SfoI GGC'GCC
Cuts at: 968 1565 1813 968
Size: 597 248 3288
Fragments arranged by size:
3288 597 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
SmaI CCC'GGG
Cuts at: 418 1155 418
Size: 737 3396
SmlI C'TyrA_G
Cuts at: 406 1288 2203 2695 2957 406
Size: 882 915 492 262 1582
Fragments arranged by size:
1582 915 882 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4133
SphI G_CATG'C
Cuts at: 1186 1686 1186
Size: 500 3633
SspI AAT'ATT
Cuts at: 573 573
Size: 4133
StuI AGG'CCT
Cuts at: 863 863
Size: 4133
StyI C'CwwG_G
Cuts at: 912 1267 1621 912
Size: 355 354 3424
Fragments arranged by size:
3424 355 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 939 1716 1849 2308 2517 939
Size: 777 133 459 209 2555
Fragments arranged by size:
2555 777 459 209 133
TatI w'GTAC_w
Cuts at: 721 3648 721
Size: 2927 1206
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3650 3682 3650
Size: 32 4101
Tth111I GACn'n_nGTC
Cuts at: 1930 1930
Size: 4133
XbaI T'CTAG_A
Cuts at: 433 483 1164 433
Size: 50 681 3402
Fragments arranged by size:
3402 681 50
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1628 1628
Size: 4133
XmaI C'CCGG_G
Cuts at: 416 1153 416
Size: 737 3396
ZraI GAC'GTC
Cuts at: 245 245
Size: 4133
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BcgI BfrBI BglI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI