Restriction map for
pF1KB7391
(Circular) (Six-base) MAPSORT of: pF1KB7391.seq Check: 2692 from: 1 to: 3806
pF1KB7391 3806 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 586 586
Size: 3806
AccI GT'mk_AC
Cuts at: 844 844
Size: 3806
Acc65I G'GTAC_C
Cuts at: 822 822
Size: 3806
AclI AA'CG_TT
Cuts at: 3646 3646
Size: 3806
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 1631 2063 2810 3078 154
Size: 1477 432 747 268 882
Fragments arranged by size:
1477 882 747 432 268
AflIII A'CryG_T
Cuts at: 388 1158 2262 3239 388
Size: 770 1104 977 955
Fragments arranged by size:
1104 977 955 770
AgeI A'CCGG_T
Cuts at: 2194 2194
Size: 3806
AlwNI CAG_nnn'CTG
Cuts at: 462 1111 2678 462
Size: 649 1567 1590
Fragments arranged by size:
1590 1567 649
ApaLI G'TGCA_C
Cuts at: 2576 2576
Size: 3806
ApoI r'AATT_y
Cuts at: 810 3159 3415 810
Size: 2349 256 1201
Fragments arranged by size:
2349 1201 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3806
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3806
AvaI C'yCGr_G
Cuts at: 826 826
Size: 3806
AvrII C'CTAG_G
Cuts at: 337 337
Size: 3806
BamHI G'GATC_C
Cuts at: 831 831
Size: 3806
BanI G'GyrC_C
Cuts at: 795 822 1236 1484 1519 795
Size: 27 414 248 35 3082
Fragments arranged by size:
3082 414 248 35 27
BanII G_rGCy'C
Cuts at: 820 1850 820
Size: 1030 2776
BbeI G_GCGC'C
Cuts at: 1240 1488 1240
Size: 248 3558
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 564 598 564
Size: 34 3772
BciVI GTATCCnnnnn_n'
Cuts at: 1704 2465 3806 1704
Size: 761 1341 1704
Fragments arranged by size:
1704 1341 761
BclI T'GATC_A
Cuts at: 1326 1326
Size: 3806
BfrBI ATG'CAT
Cuts at: 529 529
Size: 3806
BglII A'GATC_T
Cuts at: 1321 3358 1321
Size: 2037 1769
BlpI GC'TnA_GC
Cuts at: 918 918
Size: 3806
Bme1580I G_kGCm'C
Cuts at: 800 1431 1524 2580 800
Size: 631 93 1056 2026
Fragments arranged by size:
2026 1056 631 93
BmrI ACTGGGnnnn_n'
Cuts at: 1018 1099 1434 3578 1018
Size: 81 335 2144 1246
Fragments arranged by size:
2144 1246 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 3806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 698
Size: 3806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1861 2353 2651 982
Size: 879 492 298 2137
Fragments arranged by size:
2137 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 161 3510 161
Size: 3349 457
BsaAI yAC'GTr
Cuts at: 1159 1159
Size: 3806
BsaBI GATnn'nnATC
Cuts at: 6 1320 3357 6
Size: 1314 2037 455
Fragments arranged by size:
2037 1314 455
BsaHI Gr'CG_yC
Cuts at: 1237 1485 2187 3683 1237
Size: 248 702 1496 1360
Fragments arranged by size:
1496 1360 702 248
BsaWI w'CCGG_w
Cuts at: 995 1516 2194 2468 2615 3026 995
Size: 521 678 274 147 411 1775
Fragments arranged by size:
1775 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 523 523
Size: 3806
BseYI C'CCAG_C
Cuts at: 1231 1989 2566 3062 1231
Size: 758 577 496 1975
Fragments arranged by size:
1975 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 396 261
Size: 135 3671
BsiEI CG_ry'CG
Cuts at: 104 1348 1394 2602 104
Size: 1244 46 1208 1308
Fragments arranged by size:
1308 1244 1208 46
BsiHKAI G_wGCw'C
Cuts at: 820 1598 1788 2580 820
Size: 778 190 792 2046
Fragments arranged by size:
2046 792 778 190
BsmI GAATG_Cn'
Cuts at: 208 208
Size: 3806
Bsp1286I G_dGCh'C
Cuts at: 800 820 1431 1524 1598 1788 1850 2580 800
Size: 20 611 93 74 190 62 730 2026
Fragments arranged by size:
2026 730 611 190 93 74 62 20
BspEI T'CCGG_A
Cuts at: 3026 3026
Size: 3806
BspHI T'CATG_A
Cuts at: 2982 2982
Size: 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 586 856 1070 1372 1753 586
Size: 270 214 302 381 2639
Fragments arranged by size:
2639 381 302 270 214
BsrBI CCG'CTC
Cuts at: 50 2098 2152 3636 3801 50
Size: 2048 54 1484 165 55
Fragments arranged by size:
2048 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1718 1718
Size: 3806
BsrFI r'CCGG_y
Cuts at: 562 1804 1985 2194 562
Size: 1242 181 209 2174
Fragments arranged by size:
2174 1242 209 181
BssSI C'ACGA_G
Cuts at: 2077 2435 2077
Size: 358 3448
BstAPI GCAn_nnn'nTGC
Cuts at: 145 1183 145
Size: 1038 2768
BstBI TT'CG_AA
Cuts at: 2167 2167
Size: 3806
BstYI r'GATC_y
Cuts at: 1 296 788 831 1298 1321 1656 1902 2914
Size: 295 492 43 467 23 335 246 1012
Cuts at: 2914 3000 3012 3358 1
Size: 86 12 346 449
Fragments arranged by size:
1012 492 467 449 346 335 295 246
86 43 23 12
Bsu36I CC'TnA_GG
Cuts at: 314 314
Size: 3806
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1756 1937 2100 1756
Size: 181 163 3462
Fragments arranged by size:
3462 181 163
BtsI GCAGTG_nn'
Cuts at: 1049 1049
Size: 3806
DraI TTT'AAA
Cuts at: 805 805
Size: 3806
DrdI GACnn_nn'nnGTC
Cuts at: 768 1512 2370 768
Size: 744 858 2204
Fragments arranged by size:
2204 858 744
EaeI y'GGCC_r
Cuts at: 570 705 1391 1565 1956 1983 3328 570
Size: 135 686 174 391 27 1345 1048
Fragments arranged by size:
1345 1048 686 391 174 135 27
EagI C'GGCC_G
Cuts at: 1391 1391
Size: 3806
EarI CTCTTCn'nnn_
Cuts at: 1829 2039 1829
Size: 210 3596
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2014 2324 2470 3616 2014
Size: 310 146 1146 2204
Fragments arranged by size:
2204 1146 310 146
EcoICRI GAG'CTC
Cuts at: 818 818
Size: 3806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 698 1631 2063 2810 3078 154
Size: 544 933 432 747 268 882
Fragments arranged by size:
933 882 747 544 432 268
EcoO109I rG'GnC_Cy
Cuts at: 945 945
Size: 3806
EcoRI G'AATT_C
Cuts at: 810 810
Size: 3806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1825 1825
Size: 3806
FspI TGC'GCA
Cuts at: 1587 1587
Size: 3806
HaeII r_GCGC'y
Cuts at: 1240 1488 2510 3489 1240
Size: 248 1022 979 1557
Fragments arranged by size:
1557 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1648 1678 1680 1710 1648
Size: 30 2 30 3744
Fragments arranged by size:
3744 30 30 2
HincII GTy'rAC
Cuts at: 845 845
Size: 3806
KasI G'GCGC_C
Cuts at: 1236 1484 1236
Size: 248 3558
KpnI G_GTAC'C
Cuts at: 826 826
Size: 3806
MluI A'CGCG_T
Cuts at: 3239 3239
Size: 3806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 274 2477 2661 274
Size: 2203 184 1419
Fragments arranged by size:
2203 1419 184
MscI TGG'CCA
Cuts at: 1567 1567
Size: 3806
MslI CAynn'nnrTG
Cuts at: 1795 1922 1795
Size: 127 3679
MspA1I CmG'CkG
Cuts at: 490 917 1231 1591 2604 2849 490
Size: 427 314 360 1013 245 1447
Fragments arranged by size:
1447 1013 427 360 314 245
NaeI GCC'GGC
Cuts at: 1987 1987
Size: 3806
NarI GG'CG_CC
Cuts at: 1237 1485 1237
Size: 248 3558
NgoMIV G'CCGG_C
Cuts at: 1985 1985
Size: 3806
NsiI A_TGCA'T
Cuts at: 531 531
Size: 3806
NspI r_CATG'y
Cuts at: 190 392 859 1359 2266 190
Size: 202 467 500 907 1730
Fragments arranged by size:
1730 907 500 467 202
PasI CC'CwG_GG
Cuts at: 540 540
Size: 3806
PciI A'CATG_T
Cuts at: 388 2262 388
Size: 1874 1932
PfoI T'CCnGG_A
Cuts at: 166 166
Size: 3806
PmeI GTTT'AAAC
Cuts at: 805 805
Size: 3806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2945 2977 2945
Size: 32 3774
PstI C_TGCA'G
Cuts at: 180 559 853 1538 180
Size: 379 294 685 2448
Fragments arranged by size:
2448 685 379 294
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3806
PvuII CAG'CTG
Cuts at: 1231 1591 1231
Size: 360 3446
RsrII CG'GwC_CG
Cuts at: 2001 2001
Size: 3806
SacI G_AGCT'C
Cuts at: 820 820
Size: 3806
SalI G'TCGA_C
Cuts at: 843 843
Size: 3806
SapI GCTCTTCn'nnn_
Cuts at: 1829 2039 1829
Size: 210 3596
SbfI CC_TGCA'GG
Cuts at: 853 853
Size: 3806
ScaI AGT'ACT
Cuts at: 3323 3323
Size: 3806
SfcI C'TryA_G
Cuts at: 33 176 555 849 1534 2527 2718 33
Size: 143 379 294 685 993 191 1121
Fragments arranged by size:
1121 993 685 379 294 191 143
SfoI GGC'GCC
Cuts at: 1238 1486 1238
Size: 248 3558
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3806
SmaI CCC'GGG
Cuts at: 828 828
Size: 3806
SmlI C'TyrA_G
Cuts at: 961 1876 2368 2630 961
Size: 915 492 262 2137
Fragments arranged by size:
2137 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3806
SphI G_CATG'C
Cuts at: 859 1359 859
Size: 500 3306
StyI C'CwwG_G
Cuts at: 337 940 1294 337
Size: 603 354 2849
Fragments arranged by size:
2849 603 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1389 1522 1981 2190 1389
Size: 133 459 209 3005
Fragments arranged by size:
3005 459 209 133
TatI w'GTAC_w
Cuts at: 3321 3321
Size: 3806
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 3774
Tth111I GACn'n_nGTC
Cuts at: 1603 1603
Size: 3806
XbaI T'CTAG_A
Cuts at: 837 837
Size: 3806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1301 1301
Size: 3806
XmaI C'CCGG_G
Cuts at: 826 826
Size: 3806
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PasI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbsI BbvCI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
PacI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI