Restriction map for
pF1KB7390
(Circular) (Six-base) MAPSORT of: pF1KB7390.seq Check: 4152 from: 1 to: 4076
pF1KB7390 4076 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 226 226
Size: 4076
AccI GT'mk_AC
Cuts at: 1114 1114
Size: 4076
Acc65I G'GTAC_C
Cuts at: 1092 1092
Size: 4076
AclI AA'CG_TT
Cuts at: 3916 3916
Size: 4076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 618 864 1901 2333 3080 3348 321
Size: 297 246 1037 432 747 268 1049
Fragments arranged by size:
1049 1037 747 432 297 268 246
AfeI AGC'GCT
Cuts at: 475 475
Size: 4076
AflIII A'CryG_T
Cuts at: 1428 2532 3509 1428
Size: 1104 977 1995
Fragments arranged by size:
1995 1104 977
AgeI A'CCGG_T
Cuts at: 2464 2464
Size: 4076
AhdI GACnn_n'nnGTC
Cuts at: 563 563
Size: 4076
AlwNI CAG_nnn'CTG
Cuts at: 375 873 1381 2948 375
Size: 498 508 1567 1503
Fragments arranged by size:
1567 1503 508 498
ApaLI G'TGCA_C
Cuts at: 175 2846 175
Size: 2671 1405
ApoI r'AATT_y
Cuts at: 613 1080 3429 3685 613
Size: 467 2349 256 1004
Fragments arranged by size:
2349 1004 467 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
AvaI C'yCGr_G
Cuts at: 859 1018 1096 859
Size: 159 78 3839
Fragments arranged by size:
3839 159 78
BamHI G'GATC_C
Cuts at: 1101 1101
Size: 4076
BanI G'GyrC_C
Cuts at: 524 1051 1092 1506 1754 1789 524
Size: 527 41 414 248 35 2811
Fragments arranged by size:
2811 527 414 248 41 35
BanII G_rGCy'C
Cuts at: 202 733 1019 1090 2120 202
Size: 531 286 71 1030 2158
Fragments arranged by size:
2158 1030 531 286 71
BbeI G_GCGC'C
Cuts at: 1510 1758 1510
Size: 248 3828
BciVI GTATCCnnnnn_n'
Cuts at: 1974 2735 4076 1974
Size: 761 1341 1974
Fragments arranged by size:
1974 1341 761
BclI T'GATC_A
Cuts at: 1596 1596
Size: 4076
BglI GCCn_nnn'nGGC
Cuts at: 759 759
Size: 4076
BglII A'GATC_T
Cuts at: 1591 3628 1591
Size: 2037 2039
BlpI GC'TnA_GC
Cuts at: 196 355 1188 196
Size: 159 833 3084
Fragments arranged by size:
3084 833 159
Bme1580I G_kGCm'C
Cuts at: 179 1054 1701 1794 2850 179
Size: 875 647 93 1056 1405
Fragments arranged by size:
1405 1056 875 647 93
BmrI ACTGGGnnnn_n'
Cuts at: 676 1288 1369 1704 3848 676
Size: 612 81 335 2144 904
Fragments arranged by size:
2144 904 612 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4076
Bpu10I CC'TnA_GC
Cuts at: 445 445
Size: 4076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1252 2131 2623 2921 1252
Size: 879 492 298 2407
Fragments arranged by size:
2407 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3780 3780
Size: 4076
BsaAI yAC'GTr
Cuts at: 1429 1429
Size: 4076
BsaBI GATnn'nnATC
Cuts at: 6 1590 3627 6
Size: 1584 2037 455
Fragments arranged by size:
2037 1584 455
BsaHI Gr'CG_yC
Cuts at: 1039 1507 1755 2457 3953 1039
Size: 468 248 702 1496 1162
Fragments arranged by size:
1496 1162 702 468 248
BsaWI w'CCGG_w
Cuts at: 1265 1786 2464 2738 2885 3296 1265
Size: 521 678 274 147 411 2045
Fragments arranged by size:
2045 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 551 581 551
Size: 30 4046
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 316 741 888 969 1027 316
Size: 425 147 81 58 3365
Fragments arranged by size:
3365 425 147 81 58
BseYI C'CCAG_C
Cuts at: 261 368 470 734 1501 2259 2836 3332 261
Size: 107 102 264 767 758 577 496 1005
Fragments arranged by size:
1005 767 758 577 496 264 107 102
BsiEI CG_ry'CG
Cuts at: 104 1618 1664 2872 104
Size: 1514 46 1208 1308
Fragments arranged by size:
1514 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 172 179 202 733 839 995 1090 1868 2058
Size: 7 23 531 106 156 95 778 190
Cuts at: 2058 2850 172
Size: 792 1398
Fragments arranged by size:
1398 792 778 531 190 156 106 95
23 7
BsmI GAATG_Cn'
Cuts at: 278 278
Size: 4076
BsmBI CGTCTCn'nnnn_
Cuts at: 1030 1030
Size: 4076
Bsp1286I G_dGCh'C
Cuts at: 172 179 202 733 839 995 1019 1054 1090
Size: 7 23 531 106 156 24 35 36
Cuts at: 1090 1701 1794 1868 2058 2120 2850 172
Size: 611 93 74 190 62 730 1398
Fragments arranged by size:
1398 730 611 531 190 156 106 93
74 62 36 35 24 23 7
BspEI T'CCGG_A
Cuts at: 3296 3296
Size: 4076
BspHI T'CATG_A
Cuts at: 3252 3252
Size: 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 226 858 1126 1340 1642 2023 226
Size: 632 268 214 302 381 2279
Fragments arranged by size:
2279 632 381 302 268 214
BsrBI CCG'CTC
Cuts at: 50 2368 2422 3906 4071 50
Size: 2318 54 1484 165 55
Fragments arranged by size:
2318 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1988 1988
Size: 4076
BsrFI r'CCGG_y
Cuts at: 515 2074 2255 2464 515
Size: 1559 181 209 2127
Fragments arranged by size:
2127 1559 209 181
BssSI C'ACGA_G
Cuts at: 400 2347 2705 400
Size: 1947 358 1771
Fragments arranged by size:
1947 1771 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1453 1453
Size: 4076
BstBI TT'CG_AA
Cuts at: 270 2437 270
Size: 2167 1909
BstXI CCAn_nnnn'nTGG
Cuts at: 981 1062 981
Size: 81 3995
BstYI r'GATC_y
Cuts at: 1 1101 1568 1591 1926 2172 3184 3270 3282
Size: 1100 467 23 335 246 1012 86 12
Cuts at: 3282 3628 1
Size: 346 449
Fragments arranged by size:
1100 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 137 1046 137
Size: 909 3167
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2026 2207 2370 2026
Size: 181 163 3732
Fragments arranged by size:
3732 181 163
BtsI GCAGTG_nn'
Cuts at: 135 745 1319 135
Size: 610 574 2892
Fragments arranged by size:
2892 610 574
DraI TTT'AAA
Cuts at: 1075 1075
Size: 4076
DrdI GACnn_nn'nnGTC
Cuts at: 141 1782 2640 141
Size: 1641 858 1577
Fragments arranged by size:
1641 1577 858
EaeI y'GGCC_r
Cuts at: 206 458 1661 1835 2226 2253 3598 206
Size: 252 1203 174 391 27 1345 684
Fragments arranged by size:
1345 1203 684 391 252 174 27
EagI C'GGCC_G
Cuts at: 1661 1661
Size: 4076
EarI CTCTTCn'nnn_
Cuts at: 334 340 406 496 594 2099 2309 334
Size: 6 66 90 98 1505 210 2101
Fragments arranged by size:
2101 1505 210 98 90 66 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2284 2594 2740 3886 2284
Size: 310 146 1146 2474
Fragments arranged by size:
2474 1146 310 146
EcoICRI GAG'CTC
Cuts at: 200 731 1088 200
Size: 531 357 3188
Fragments arranged by size:
3188 531 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 618 864 1901 2333 3080 3348 321
Size: 297 246 1037 432 747 268 1049
Fragments arranged by size:
1049 1037 747 432 297 268 246
EcoO109I rG'GnC_Cy
Cuts at: 870 977 1215 870
Size: 107 238 3731
Fragments arranged by size:
3731 238 107
EcoRI G'AATT_C
Cuts at: 1080 1080
Size: 4076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 4076
FspI TGC'GCA
Cuts at: 1857 1857
Size: 4076
HaeII r_GCGC'y
Cuts at: 477 920 1006 1510 1758 2780 3759 477
Size: 443 86 504 248 1022 979 794
Fragments arranged by size:
1022 979 794 504 443 248 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 549 581 1918 1948 1950 1980 549
Size: 32 1337 30 2 30 2645
Fragments arranged by size:
2645 1337 32 30 30 2
HincII GTy'rAC
Cuts at: 584 1115 584
Size: 531 3545
KasI G'GCGC_C
Cuts at: 1506 1754 1506
Size: 248 3828
KpnI G_GTAC'C
Cuts at: 1096 1096
Size: 4076
MluI A'CGCG_T
Cuts at: 3509 3509
Size: 4076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1020 2747 2931 1020
Size: 1727 184 2165
Fragments arranged by size:
2165 1727 184
MscI TGG'CCA
Cuts at: 208 1837 208
Size: 1629 2447
MslI CAynn'nnrTG
Cuts at: 2065 2192 2065
Size: 127 3949
MspA1I CmG'CkG
Cuts at: 1187 1501 1861 2874 3119 1187
Size: 314 360 1013 245 2144
Fragments arranged by size:
2144 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 4076
NarI GG'CG_CC
Cuts at: 1507 1755 1507
Size: 248 3828
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 4076
NruI TCG'CGA
Cuts at: 1010 1010
Size: 4076
NspI r_CATG'y
Cuts at: 1129 1629 2536 1129
Size: 500 907 2669
Fragments arranged by size:
2669 907 500
PasI CC'CwG_GG
Cuts at: 981 981
Size: 4076
PciI A'CATG_T
Cuts at: 2532 2532
Size: 4076
PflMI CCAn_nnn'nTGG
Cuts at: 375 375
Size: 4076
PmeI GTTT'AAAC
Cuts at: 1075 1075
Size: 4076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 4044
PpuMI rG'GwC_Cy
Cuts at: 870 977 870
Size: 107 3969
PstI C_TGCA'G
Cuts at: 601 1123 1808 601
Size: 522 685 2869
Fragments arranged by size:
2869 685 522
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4076
PvuII CAG'CTG
Cuts at: 1501 1861 1501
Size: 360 3716
RsrII CG'GwC_CG
Cuts at: 2271 2271
Size: 4076
SacI G_AGCT'C
Cuts at: 202 733 1090 202
Size: 531 357 3188
Fragments arranged by size:
3188 531 357
SalI G'TCGA_C
Cuts at: 1113 1113
Size: 4076
SapI GCTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
SbfI CC_TGCA'GG
Cuts at: 1123 1123
Size: 4076
ScaI AGT'ACT
Cuts at: 3593 3593
Size: 4076
SexAI A'CCwGG_T
Cuts at: 188 973 188
Size: 785 3291
SfcI C'TryA_G
Cuts at: 33 597 1119 1804 2797 2988 33
Size: 564 522 685 993 191 1121
Fragments arranged by size:
1121 993 685 564 522 191
SfoI GGC'GCC
Cuts at: 1508 1756 1508
Size: 248 3828
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
SmaI CCC'GGG
Cuts at: 1098 1098
Size: 4076
SmlI C'TyrA_G
Cuts at: 1231 2146 2638 2900 1231
Size: 915 492 262 2407
Fragments arranged by size:
2407 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4076
SphI G_CATG'C
Cuts at: 1129 1629 1129
Size: 500 3576
StyI C'CwwG_G
Cuts at: 1210 1564 1210
Size: 354 3722
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 484 528 708 1659 1792 2251 2460 484
Size: 44 180 951 133 459 209 2100
Fragments arranged by size:
2100 951 459 209 180 133 44
TatI w'GTAC_w
Cuts at: 422 3591 422
Size: 3169 907
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 551 583 3593 3625 551
Size: 32 3010 32 1002
Fragments arranged by size:
3010 1002 32 32
Tth111I GACn'n_nGTC
Cuts at: 394 646 693 1873 394
Size: 252 47 1180 2597
Fragments arranged by size:
2597 1180 252 47
XbaI T'CTAG_A
Cuts at: 1107 1107
Size: 4076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 357 978 1571 357
Size: 621 593 2862
Fragments arranged by size:
2862 621 593
XmaI C'CCGG_G
Cuts at: 1096 1096
Size: 4076
XmnI GAAnn'nnTTC
Cuts at: 286 286
Size: 4076
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NruI NspI PasI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BpmI
BsgI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NsiI PacI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI