Restriction map for
pF1KB7389
(Circular) (Six-base) MAPSORT of: pF1KB7389.seq Check: 1088 from: 1 to: 3728
pF1KB7389 3728 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 596 766 596
Size: 170 3558
Acc65I G'GTAC_C
Cuts at: 744 744
Size: 3728
AclI AA'CG_TT
Cuts at: 3568 3568
Size: 3728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1553 1985 2732 3000 649
Size: 904 432 747 268 1377
Fragments arranged by size:
1377 904 747 432 268
AflIII A'CryG_T
Cuts at: 1080 2184 3161 1080
Size: 1104 977 1647
Fragments arranged by size:
1647 1104 977
AgeI A'CCGG_T
Cuts at: 2116 2116
Size: 3728
AlwNI CAG_nnn'CTG
Cuts at: 543 1033 2600 543
Size: 490 1567 1671
Fragments arranged by size:
1671 1567 490
ApaLI G'TGCA_C
Cuts at: 2498 2498
Size: 3728
ApoI r'AATT_y
Cuts at: 732 3081 3337 732
Size: 2349 256 1123
Fragments arranged by size:
2349 1123 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3728
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
AvaI C'yCGr_G
Cuts at: 748 748
Size: 3728
AvrII C'CTAG_G
Cuts at: 717 717
Size: 3728
BamHI G'GATC_C
Cuts at: 753 753
Size: 3728
BanI G'GyrC_C
Cuts at: 517 744 1158 1406 1441 517
Size: 227 414 248 35 2804
Fragments arranged by size:
2804 414 248 227 35
BanII G_rGCy'C
Cuts at: 356 514 742 1772 356
Size: 158 228 1030 2312
Fragments arranged by size:
2312 1030 228 158
BbeI G_GCGC'C
Cuts at: 1162 1410 1162
Size: 248 3480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 565 599 565
Size: 34 3694
BciVI GTATCCnnnnn_n'
Cuts at: 1626 2387 3728 1626
Size: 761 1341 1626
Fragments arranged by size:
1626 1341 761
BclI T'GATC_A
Cuts at: 1248 1248
Size: 3728
BglII A'GATC_T
Cuts at: 1243 3280 1243
Size: 2037 1691
BlpI GC'TnA_GC
Cuts at: 840 840
Size: 3728
Bme1580I G_kGCm'C
Cuts at: 522 1353 1446 2502 522
Size: 831 93 1056 1748
Fragments arranged by size:
1748 1056 831 93
BmgBI CAC'GTC
Cuts at: 221 221
Size: 3728
BmrI ACTGGGnnnn_n'
Cuts at: 645 940 1021 1356 3500 645
Size: 295 81 335 2144 873
Fragments arranged by size:
2144 873 335 295 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 3728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 682 370
Size: 312 3416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 1783 2275 2573 904
Size: 879 492 298 2059
Fragments arranged by size:
2059 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3432 3432
Size: 3728
BsaAI yAC'GTr
Cuts at: 680 1081 680
Size: 401 3327
BsaBI GATnn'nnATC
Cuts at: 6 445 1242 3279 6
Size: 439 797 2037 455
Fragments arranged by size:
2037 797 455 439
BsaHI Gr'CG_yC
Cuts at: 1159 1407 2109 3605 1159
Size: 248 702 1496 1282
Fragments arranged by size:
1496 1282 702 248
BsaWI w'CCGG_w
Cuts at: 917 1438 2116 2390 2537 2948 917
Size: 521 678 274 147 411 1697
Fragments arranged by size:
1697 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1153 1911 2488 2984 1153
Size: 758 577 496 1897
Fragments arranged by size:
1897 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 635
Size: 3728
BsiEI CG_ry'CG
Cuts at: 104 1270 1316 2524 104
Size: 1166 46 1208 1308
Fragments arranged by size:
1308 1208 1166 46
BsiHKAI G_wGCw'C
Cuts at: 356 679 742 1520 1710 2502 356
Size: 323 63 778 190 792 1582
Fragments arranged by size:
1582 792 778 323 190 63
Bsp1286I G_dGCh'C
Cuts at: 356 514 522 679 742 1353 1446 1520 1710
Size: 158 8 157 63 611 93 74 190
Cuts at: 1710 1772 2502 356
Size: 62 730 1582
Fragments arranged by size:
1582 730 611 190 158 157 93 74
63 62 8
BspEI T'CCGG_A
Cuts at: 2948 2948
Size: 3728
BspHI T'CATG_A
Cuts at: 2904 2904
Size: 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 778 992 1294 1675 778
Size: 214 302 381 2831
Fragments arranged by size:
2831 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2020 2074 3558 3723 50
Size: 1970 54 1484 165 55
Fragments arranged by size:
1970 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1640 1640
Size: 3728
BsrFI r'CCGG_y
Cuts at: 1726 1907 2116 1726
Size: 181 209 3338
Fragments arranged by size:
3338 209 181
BssSI C'ACGA_G
Cuts at: 1999 2357 1999
Size: 358 3370
BstAPI GCAn_nnn'nTGC
Cuts at: 1105 1105
Size: 3728
BstBI TT'CG_AA
Cuts at: 2089 2089
Size: 3728
BstEII G'GTnAC_C
Cuts at: 325 325
Size: 3728
BstXI CCAn_nnnn'nTGG
Cuts at: 396 396
Size: 3728
BstYI r'GATC_y
Cuts at: 1 167 753 1220 1243 1578 1824 2836 2922
Size: 166 586 467 23 335 246 1012 86
Cuts at: 2922 2934 3280 1
Size: 12 346 449
Fragments arranged by size:
1012 586 467 449 346 335 246 166
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1678 1859 2022 1678
Size: 181 163 3384
Fragments arranged by size:
3384 181 163
BtsI GCAGTG_nn'
Cuts at: 425 971 425
Size: 546 3182
DraI TTT'AAA
Cuts at: 245 727 245
Size: 482 3246
DrdI GACnn_nn'nnGTC
Cuts at: 1434 2292 1434
Size: 858 2870
EaeI y'GGCC_r
Cuts at: 363 1313 1487 1878 1905 3250 363
Size: 950 174 391 27 1345 841
Fragments arranged by size:
1345 950 841 391 174 27
EagI C'GGCC_G
Cuts at: 1313 1313
Size: 3728
EarI CTCTTCn'nnn_
Cuts at: 361 489 1751 1961 361
Size: 128 1262 210 2128
Fragments arranged by size:
2128 1262 210 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1936 2246 2392 3538 1936
Size: 310 146 1146 2126
Fragments arranged by size:
2126 1146 310 146
EcoICRI GAG'CTC
Cuts at: 354 740 354
Size: 386 3342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 649 682 1553 1985 2732 3000 370
Size: 279 33 871 432 747 268 1098
Fragments arranged by size:
1098 871 747 432 279 268 33
EcoO109I rG'GnC_Cy
Cuts at: 333 704 867 333
Size: 371 163 3194
Fragments arranged by size:
3194 371 163
EcoRI G'AATT_C
Cuts at: 732 732
Size: 3728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1747 1747
Size: 3728
FspI TGC'GCA
Cuts at: 1509 1509
Size: 3728
HaeII r_GCGC'y
Cuts at: 1162 1410 2432 3411 1162
Size: 248 1022 979 1479
Fragments arranged by size:
1479 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1570 1600 1602 1632 1570
Size: 30 2 30 3666
Fragments arranged by size:
3666 30 30 2
HincII GTy'rAC
Cuts at: 767 767
Size: 3728
KasI G'GCGC_C
Cuts at: 1158 1406 1158
Size: 248 3480
KpnI G_GTAC'C
Cuts at: 748 748
Size: 3728
MluI A'CGCG_T
Cuts at: 3161 3161
Size: 3728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 181 238 2399 2583 181
Size: 57 2161 184 1326
Fragments arranged by size:
2161 1326 184 57
MscI TGG'CCA
Cuts at: 1489 1489
Size: 3728
MslI CAynn'nnrTG
Cuts at: 394 1717 1844 394
Size: 1323 127 2278
Fragments arranged by size:
2278 1323 127
MspA1I CmG'CkG
Cuts at: 627 839 1153 1513 2526 2771 627
Size: 212 314 360 1013 245 1584
Fragments arranged by size:
1584 1013 360 314 245 212
NaeI GCC'GGC
Cuts at: 1909 1909
Size: 3728
NarI GG'CG_CC
Cuts at: 1159 1407 1159
Size: 248 3480
NgoMIV G'CCGG_C
Cuts at: 1907 1907
Size: 3728
NspI r_CATG'y
Cuts at: 149 781 1281 2188 149
Size: 632 500 907 1689
Fragments arranged by size:
1689 907 632 500
PciI A'CATG_T
Cuts at: 2184 2184
Size: 3728
PmeI GTTT'AAAC
Cuts at: 727 727
Size: 3728
PmlI CAC'GTG
Cuts at: 680 680
Size: 3728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 3696
PpuMI rG'GwC_Cy
Cuts at: 333 704 333
Size: 371 3357
PstI C_TGCA'G
Cuts at: 775 1460 775
Size: 685 3043
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3728
PvuII CAG'CTG
Cuts at: 627 1153 1513 627
Size: 526 360 2842
Fragments arranged by size:
2842 526 360
RsrII CG'GwC_CG
Cuts at: 1923 1923
Size: 3728
SacI G_AGCT'C
Cuts at: 356 742 356
Size: 386 3342
SalI G'TCGA_C
Cuts at: 765 765
Size: 3728
SapI GCTCTTCn'nnn_
Cuts at: 361 1751 1961 361
Size: 1390 210 2128
Fragments arranged by size:
2128 1390 210
SbfI CC_TGCA'GG
Cuts at: 775 775
Size: 3728
ScaI AGT'ACT
Cuts at: 3245 3245
Size: 3728
SfcI C'TryA_G
Cuts at: 33 213 771 1456 2449 2640 33
Size: 180 558 685 993 191 1121
Fragments arranged by size:
1121 993 685 558 191 180
SfoI GGC'GCC
Cuts at: 1160 1408 1160
Size: 248 3480
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
SmaI CCC'GGG
Cuts at: 750 750
Size: 3728
SmlI C'TyrA_G
Cuts at: 883 1798 2290 2552 883
Size: 915 492 262 2059
Fragments arranged by size:
2059 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3728
SphI G_CATG'C
Cuts at: 781 1281 781
Size: 500 3228
StyI C'CwwG_G
Cuts at: 717 862 1216 717
Size: 145 354 3229
Fragments arranged by size:
3229 354 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 185 478 1311 1444 1903 2112 185
Size: 293 833 133 459 209 1801
Fragments arranged by size:
1801 833 459 293 209 133
TatI w'GTAC_w
Cuts at: 407 3243 407
Size: 2836 892
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 181 213 3245 3277 181
Size: 32 3032 32 632
Fragments arranged by size:
3032 632 32 32
Tth111I GACn'n_nGTC
Cuts at: 1525 1525
Size: 3728
XbaI T'CTAG_A
Cuts at: 759 759
Size: 3728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1223 1223
Size: 3728
XmaI C'CCGG_G
Cuts at: 748 748
Size: 3728
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbsI BbvCI BfrBI BglI BmtI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI