Restriction map for
pF1KB7387
(Circular) (Six-base) MAPSORT of: pF1KB7387.seq Check: 600 from: 1 to: 4403
pF1KB7387 4403 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1441 1441
Size: 4403
Acc65I G'GTAC_C
Cuts at: 1419 1419
Size: 4403
AclI AA'CG_TT
Cuts at: 4243 4243
Size: 4403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 852 1188 2228 2660 3407 3675 852
Size: 336 1040 432 747 268 1580
Fragments arranged by size:
1580 1040 747 432 336 268
AflIII A'CryG_T
Cuts at: 1755 2859 3836 1755
Size: 1104 977 2322
Fragments arranged by size:
2322 1104 977
AgeI A'CCGG_T
Cuts at: 2791 2791
Size: 4403
AlwNI CAG_nnn'CTG
Cuts at: 482 1708 3275 482
Size: 1226 1567 1610
Fragments arranged by size:
1610 1567 1226
ApaLI G'TGCA_C
Cuts at: 1298 3173 1298
Size: 1875 2528
ApoI r'AATT_y
Cuts at: 619 665 690 986 1407 3756 4012 619
Size: 46 25 296 421 2349 256 1010
Fragments arranged by size:
2349 1010 421 296 256 46 25
AseI AT'TA_AT
Cuts at: 20 20
Size: 4403
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
AvaI C'yCGr_G
Cuts at: 1331 1423 1331
Size: 92 4311
AvrII C'CTAG_G
Cuts at: 495 495
Size: 4403
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 268 301 268
Size: 33 4370
BamHI G'GATC_C
Cuts at: 1428 1428
Size: 4403
BanI G'GyrC_C
Cuts at: 1419 1833 2081 2116 1419
Size: 414 248 35 3706
Fragments arranged by size:
3706 414 248 35
BanII G_rGCy'C
Cuts at: 1417 2447 1417
Size: 1030 3373
BbeI G_GCGC'C
Cuts at: 1837 2085 1837
Size: 248 4155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1351 1385 1351
Size: 34 4369
BciVI GTATCCnnnnn_n'
Cuts at: 357 956 2301 3062 4403 357
Size: 599 1345 761 1341 357
Fragments arranged by size:
1345 1341 761 599 357
BclI T'GATC_A
Cuts at: 822 1923 822
Size: 1101 3302
BglII A'GATC_T
Cuts at: 148 1918 3955 148
Size: 1770 2037 596
Fragments arranged by size:
2037 1770 596
BlpI GC'TnA_GC
Cuts at: 1515 1515
Size: 4403
Bme1580I G_kGCm'C
Cuts at: 1302 2028 2121 3177 1302
Size: 726 93 1056 2528
Fragments arranged by size:
2528 1056 726 93
BmgBI CAC'GTC
Cuts at: 246 246
Size: 4403
BmrI ACTGGGnnnn_n'
Cuts at: 289 842 1615 1696 2031 4175 289
Size: 553 773 81 335 2144 517
Fragments arranged by size:
2144 773 553 517 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 4403
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 1100 274
Size: 826 3577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1579 2458 2950 3248 628
Size: 951 879 492 298 1783
Fragments arranged by size:
1783 951 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4107 4107
Size: 4403
BsaAI yAC'GTr
Cuts at: 756 1756 756
Size: 1000 3403
BsaBI GATnn'nnATC
Cuts at: 6 368 434 521 1917 3954 6
Size: 362 66 87 1396 2037 455
Fragments arranged by size:
2037 1396 455 362 87 66
BsaHI Gr'CG_yC
Cuts at: 1834 2082 2784 4280 1834
Size: 248 702 1496 1957
Fragments arranged by size:
1957 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1162 1592 2113 2791 3065 3212 3623 1162
Size: 430 521 678 274 147 411 1942
Fragments arranged by size:
1942 678 521 430 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 214 127
Size: 87 4316
BseYI C'CCAG_C
Cuts at: 1828 2586 3163 3659 1828
Size: 758 577 496 2572
Fragments arranged by size:
2572 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1945 1991 3199 104
Size: 1841 46 1208 1308
Fragments arranged by size:
1841 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1302 1417 2195 2385 3177 1302
Size: 115 778 190 792 2528
Fragments arranged by size:
2528 792 778 190 115
BsmI GAATG_Cn'
Cuts at: 1044 1044
Size: 4403
BsmBI CGTCTCn'nnnn_
Cuts at: 236 236
Size: 4403
Bsp1286I G_dGCh'C
Cuts at: 1302 1417 2028 2121 2195 2385 2447 3177 1302
Size: 115 611 93 74 190 62 730 2528
Fragments arranged by size:
2528 730 611 190 115 93 74 62
BspEI T'CCGG_A
Cuts at: 3623 3623
Size: 4403
BspHI T'CATG_A
Cuts at: 3579 3579
Size: 4403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1453 1667 1969 2350 1453
Size: 214 302 381 3506
Fragments arranged by size:
3506 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2695 2749 4233 4398 50
Size: 2645 54 1484 165 55
Fragments arranged by size:
2645 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1023 2315 1023
Size: 1292 3111
BsrFI r'CCGG_y
Cuts at: 2401 2582 2791 2401
Size: 181 209 4013
Fragments arranged by size:
4013 209 181
BssSI C'ACGA_G
Cuts at: 2674 3032 2674
Size: 358 4045
BstAPI GCAn_nnn'nTGC
Cuts at: 1780 1780
Size: 4403
BstBI TT'CG_AA
Cuts at: 2764 2764
Size: 4403
BstYI r'GATC_y
Cuts at: 1 148 1428 1895 1918 2253 2499 3511 3597
Size: 147 1280 467 23 335 246 1012 86
Cuts at: 3597 3609 3955 1
Size: 12 346 449
Fragments arranged by size:
1280 1012 467 449 346 335 246 147
86 23 12
BtgI C'CryG_G
Cuts at: 107 182 107
Size: 75 4328
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2353 2534 2697 2353
Size: 181 163 4059
Fragments arranged by size:
4059 181 163
BtsI GCAGTG_nn'
Cuts at: 1646 1646
Size: 4403
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1005 1040 1005
Size: 35 4368
DraI TTT'AAA
Cuts at: 1402 1402
Size: 4403
DrdI GACnn_nn'nnGTC
Cuts at: 2109 2967 2109
Size: 858 3545
EaeI y'GGCC_r
Cuts at: 179 1988 2162 2553 2580 3925 179
Size: 1809 174 391 27 1345 657
Fragments arranged by size:
1809 1345 657 391 174 27
EagI C'GGCC_G
Cuts at: 1988 1988
Size: 4403
EarI CTCTTCn'nnn_
Cuts at: 2426 2636 2426
Size: 210 4193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2611 2921 3067 4213 2611
Size: 310 146 1146 2801
Fragments arranged by size:
2801 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1415 1415
Size: 4403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 852 1100 1188 2228 2660 3407 3675 274
Size: 578 248 88 1040 432 747 268 1002
Fragments arranged by size:
1040 1002 747 578 432 268 248 88
EcoO109I rG'GnC_Cy
Cuts at: 1542 1542
Size: 4403
EcoRI G'AATT_C
Cuts at: 665 690 986 1407 665
Size: 25 296 421 3661
Fragments arranged by size:
3661 421 296 25
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4403
FspI TGC'GCA
Cuts at: 2184 2184
Size: 4403
HaeII r_GCGC'y
Cuts at: 1837 2085 3107 4086 1837
Size: 248 1022 979 2154
Fragments arranged by size:
2154 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 380 412 726 758 951 983 1203 1235 2245
Size: 32 314 32 193 32 220 32 1010
Cuts at: 2245 2275 2277 2307 380
Size: 30 2 30 2476
Fragments arranged by size:
2476 1010 314 220 193 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 407 1442 407
Size: 1035 3368
HindIII A'AGCT_T
Cuts at: 1115 1115
Size: 4403
KasI G'GCGC_C
Cuts at: 1833 2081 1833
Size: 248 4155
KpnI G_GTAC'C
Cuts at: 1423 1423
Size: 4403
MluI A'CGCG_T
Cuts at: 3836 3836
Size: 4403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 299 3074 3258 299
Size: 2775 184 1444
Fragments arranged by size:
2775 1444 184
MscI TGG'CCA
Cuts at: 2164 2164
Size: 4403
MslI CAynn'nnrTG
Cuts at: 374 2392 2519 374
Size: 2018 127 2258
Fragments arranged by size:
2258 2018 127
MspA1I CmG'CkG
Cuts at: 213 1514 1828 2188 3201 3446 213
Size: 1301 314 360 1013 245 1170
Fragments arranged by size:
1301 1170 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2584 2584
Size: 4403
NarI GG'CG_CC
Cuts at: 1834 2082 1834
Size: 248 4155
NcoI C'CATG_G
Cuts at: 107 107
Size: 4403
NgoMIV G'CCGG_C
Cuts at: 2582 2582
Size: 4403
NspI r_CATG'y
Cuts at: 1456 1956 2863 1456
Size: 500 907 2996
Fragments arranged by size:
2996 907 500
PciI A'CATG_T
Cuts at: 2859 2859
Size: 4403
PmeI GTTT'AAAC
Cuts at: 1402 1402
Size: 4403
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 3542
Size: 32 4371
PstI C_TGCA'G
Cuts at: 840 1450 2135 840
Size: 610 685 3108
Fragments arranged by size:
3108 685 610
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4403
PvuII CAG'CTG
Cuts at: 213 1828 2188 213
Size: 1615 360 2428
Fragments arranged by size:
2428 1615 360
RsrII CG'GwC_CG
Cuts at: 2598 2598
Size: 4403
SacI G_AGCT'C
Cuts at: 1417 1417
Size: 4403
SalI G'TCGA_C
Cuts at: 1440 1440
Size: 4403
SapI GCTCTTCn'nnn_
Cuts at: 2426 2636 2426
Size: 210 4193
SbfI CC_TGCA'GG
Cuts at: 1450 1450
Size: 4403
ScaI AGT'ACT
Cuts at: 3920 3920
Size: 4403
SfcI C'TryA_G
Cuts at: 33 261 836 1446 2131 3124 3315 33
Size: 228 575 610 685 993 191 1121
Fragments arranged by size:
1121 993 685 610 575 228 191
SfoI GGC'GCC
Cuts at: 1835 2083 1835
Size: 248 4155
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
SmaI CCC'GGG
Cuts at: 1425 1425
Size: 4403
SmlI C'TyrA_G
Cuts at: 607 1558 2473 2965 3227 607
Size: 951 915 492 262 1783
Fragments arranged by size:
1783 951 915 492 262
SnaBI TAC'GTA
Cuts at: 756 756
Size: 4403
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4403
SphI G_CATG'C
Cuts at: 1456 1956 1456
Size: 500 3903
SspI AAT'ATT
Cuts at: 423 423
Size: 4403
StuI AGG'CCT
Cuts at: 865 865
Size: 4403
StyI C'CwwG_G
Cuts at: 107 495 1537 1891 107
Size: 388 1042 354 2619
Fragments arranged by size:
2619 1042 388 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 327 1986 2119 2578 2787 327
Size: 1659 133 459 209 1943
Fragments arranged by size:
1943 1659 459 209 133
TatI w'GTAC_w
Cuts at: 3918 3918
Size: 4403
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3920 3952 3920
Size: 32 4371
Tth111I GACn'n_nGTC
Cuts at: 2200 2200
Size: 4403
XbaI T'CTAG_A
Cuts at: 1434 1434
Size: 4403
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1898 1898
Size: 4403
XmaI C'CCGG_G
Cuts at: 1423 1423
Size: 4403
XmnI GAAnn'nnTTC
Cuts at: 669 865 1033 669
Size: 196 168 4039
Fragments arranged by size:
4039 196 168
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BbsI BbvCI BfrBI BglI BmtI Bpu10I
BsaXI BsgI BsiWI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SwaI
XhoI ZraI