Restriction map for
pF1KB7386
(Circular) (Six-base) MAPSORT of: pF1KB7386.seq Check: 1796 from: 1 to: 4367
pF1KB7386 4367 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 189 189
Size: 4367
AccI GT'mk_AC
Cuts at: 1405 1405
Size: 4367
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 632 743 2192 2624 3371 3639 632
Size: 111 1449 432 747 268 1360
Fragments arranged by size:
1449 1360 747 432 268 111
AflIII A'CryG_T
Cuts at: 1719 2823 3800 1719
Size: 1104 977 2286
Fragments arranged by size:
2286 1104 977
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 408 766 1141 1672 3239 408
Size: 358 375 531 1567 1536
Fragments arranged by size:
1567 1536 531 375 358
ApaLI G'TGCA_C
Cuts at: 1323 3137 1323
Size: 1814 2553
ApoI r'AATT_y
Cuts at: 241 601 714 901 1371 3720 3976 241
Size: 360 113 187 470 2349 256 632
Fragments arranged by size:
2349 632 470 360 256 187 113
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 1387 1387
Size: 4367
BamHI G'GATC_C
Cuts at: 733 1392 733
Size: 659 3708
BanI G'GyrC_C
Cuts at: 1293 1383 1797 2045 2080 1293
Size: 90 414 248 35 3580
Fragments arranged by size:
3580 414 248 90 35
BanII G_rGCy'C
Cuts at: 1381 2411 1381
Size: 1030 3337
BbeI G_GCGC'C
Cuts at: 1801 2049 1801
Size: 248 4119
BbsI GAAGACnn'nnnn_
Cuts at: 128 1022 128
Size: 894 3473
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BglII A'GATC_T
Cuts at: 1882 3919 1882
Size: 2037 2330
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 1327 1992 2085 3141 1327
Size: 665 93 1056 2553
Fragments arranged by size:
2553 1056 665 93
BmrI ACTGGGnnnn_n'
Cuts at: 1579 1660 1995 4139 1579
Size: 81 335 2144 1807
Fragments arranged by size:
2144 1807 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 518
Size: 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 782 1543 2422 2914 3212 408
Size: 374 761 879 492 298 1563
Fragments arranged by size:
1563 879 761 492 374 298
BsaI GGTCTCn'nnnn_
Cuts at: 4071 4071
Size: 4367
BsaAI yAC'GTr
Cuts at: 1720 1720
Size: 4367
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 1798 2046 2748 4244 1798
Size: 248 702 1496 1921
Fragments arranged by size:
1921 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1556 2077 2755 3029 3176 3587 1556
Size: 521 678 274 147 411 2336
Fragments arranged by size:
2336 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1047 1050 1047
Size: 3 4364
BseYI C'CCAG_C
Cuts at: 1792 2550 3127 3623 1792
Size: 758 577 496 2536
Fragments arranged by size:
2536 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 791 223
Size: 568 3799
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1327 1381 2159 2349 3141 1327
Size: 54 778 190 792 2553
Fragments arranged by size:
2553 792 778 190 54
BsmI GAATG_Cn'
Cuts at: 213 258 975 213
Size: 45 717 3605
Fragments arranged by size:
3605 717 45
Bsp1286I G_dGCh'C
Cuts at: 1327 1381 1992 2085 2159 2349 2411 3141 1327
Size: 54 611 93 74 190 62 730 2553
Fragments arranged by size:
2553 730 611 190 93 74 62 54
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 3543 3543
Size: 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 189 203 1417 1631 1933 2314 189
Size: 14 1214 214 302 381 2242
Fragments arranged by size:
2242 1214 381 302 214 14
BsrBI CCG'CTC
Cuts at: 50 2659 2713 4197 4362 50
Size: 2609 54 1484 165 55
Fragments arranged by size:
2609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 218 1340 2279 218
Size: 1122 939 2306
Fragments arranged by size:
2306 1122 939
BsrFI r'CCGG_y
Cuts at: 2365 2546 2755 2365
Size: 181 209 3977
Fragments arranged by size:
3977 209 181
BsrGI T'GTAC_A
Cuts at: 1101 1101
Size: 4367
BssSI C'ACGA_G
Cuts at: 2638 2996 2638
Size: 358 4009
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstYI r'GATC_y
Cuts at: 1 156 733 1392 1859 1882 2217 2463 3475
Size: 155 577 659 467 23 335 246 1012
Cuts at: 3475 3561 3573 3919 1
Size: 86 12 346 449
Fragments arranged by size:
1012 659 577 467 449 346 335 246
155 86 23 12
BtgI C'CryG_G
Cuts at: 785 785
Size: 4367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 1610 1325
Size: 285 4082
DraI TTT'AAA
Cuts at: 420 606 851 1366 420
Size: 186 245 515 3421
Fragments arranged by size:
3421 515 245 186
DraIII CAC_nnn'GTG
Cuts at: 1000 1000
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 2073 2931 2073
Size: 858 3509
EaeI y'GGCC_r
Cuts at: 911 1952 2126 2517 2544 3889 911
Size: 1041 174 391 27 1345 1389
Fragments arranged by size:
1389 1345 1041 391 174 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 278 623 696 1068 1333 1338 2390 2600 278
Size: 345 73 372 265 5 1052 210 2045
Fragments arranged by size:
2045 1052 372 345 265 210 73 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 2885 3031 4177 2575
Size: 310 146 1146 2765
Fragments arranged by size:
2765 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 632 743 2192 2624 3371 3639 518
Size: 114 111 1449 432 747 268 1246
Fragments arranged by size:
1449 1246 747 432 268 114 111
EcoO109I rG'GnC_Cy
Cuts at: 1506 1506
Size: 4367
EcoRI G'AATT_C
Cuts at: 241 714 1371 241
Size: 473 657 3237
Fragments arranged by size:
3237 657 473
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4367
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 1801 2049 3071 4050 1801
Size: 248 1022 979 2118
Fragments arranged by size:
2118 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 457 489 567 599 2209 2239 2241 2271 457
Size: 32 78 32 1610 30 2 30 2553
Fragments arranged by size:
2553 1610 78 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1406 1406
Size: 4367
HindIII A'AGCT_T
Cuts at: 320 320
Size: 4367
KasI G'GCGC_C
Cuts at: 1797 2045 1797
Size: 248 4119
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 583 1260 3038 3222 583
Size: 677 1778 184 1728
Fragments arranged by size:
1778 1728 677 184
MscI TGG'CCA
Cuts at: 913 2128 913
Size: 1215 3152
MslI CAynn'nnrTG
Cuts at: 178 2356 2483 178
Size: 2178 127 2062
Fragments arranged by size:
2178 2062 127
MspA1I CmG'CkG
Cuts at: 793 1478 1792 2152 3165 3410 793
Size: 685 314 360 1013 245 1750
Fragments arranged by size:
1750 1013 685 360 314 245
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 1798 2046 1798
Size: 248 4119
NcoI C'CATG_G
Cuts at: 785 785
Size: 4367
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NspI r_CATG'y
Cuts at: 1420 1920 2827 1420
Size: 500 907 2960
Fragments arranged by size:
2960 907 500
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PflMI CCAn_nnn'nTGG
Cuts at: 729 729
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PstI C_TGCA'G
Cuts at: 200 1414 2099 200
Size: 1214 685 2468
Fragments arranged by size:
2468 1214 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 793 1792 2152 793
Size: 999 360 3008
Fragments arranged by size:
3008 999 360
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 278 623 2390 2600 278
Size: 345 1767 210 2045
Fragments arranged by size:
2045 1767 345 210
SbfI CC_TGCA'GG
Cuts at: 200 1414 200
Size: 1214 3153
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 196 812 1127 1253 1410 2095 3088 3279
Size: 163 616 315 126 157 685 993 191
Cuts at: 3279 33
Size: 1121
Fragments arranged by size:
1121 993 685 616 315 191 163 157
126
SfoI GGC'GCC
Cuts at: 1799 2047 1799
Size: 248 4119
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 387 797 1522 2437 2929 3191 387
Size: 410 725 915 492 262 1563
Fragments arranged by size:
1563 915 725 492 410 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
SspI AAT'ATT
Cuts at: 461 461
Size: 4367
StyI C'CwwG_G
Cuts at: 785 1501 1855 785
Size: 716 354 3297
Fragments arranged by size:
3297 716 354
SwaI ATTT'AAAT
Cuts at: 606 606
Size: 4367
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1950 2083 2542 2751 1950
Size: 133 459 209 3566
Fragments arranged by size:
3566 459 209 133
TatI w'GTAC_w
Cuts at: 1101 3882 1101
Size: 2781 1586
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3884 3916 3884
Size: 32 4335
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1304 1862 1304
Size: 558 3809
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
XmnI GAAnn'nnTTC
Cuts at: 617 617
Size: 4367
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI XhoI ZraI