Restriction map for
pF1KB7385
(Circular) (Six-base) MAPSORT of: pF1KB7385.seq Check: 4223 from: 1 to: 4064
pF1KB7385 4064 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 334 1102 334
Size: 768 3296
Acc65I G'GTAC_C
Cuts at: 1080 1080
Size: 4064
AclI AA'CG_TT
Cuts at: 3904 3904
Size: 4064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1889 2321 3068 3336 1889
Size: 432 747 268 2617
Fragments arranged by size:
2617 747 432 268
AflIII A'CryG_T
Cuts at: 183 679 1416 2520 3497 183
Size: 496 737 1104 977 750
Fragments arranged by size:
1104 977 750 737 496
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AleI CACnn'nnGTG
Cuts at: 851 851
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 1369 2936 1369
Size: 1567 2497
ApaLI G'TGCA_C
Cuts at: 2834 2834
Size: 4064
ApoI r'AATT_y
Cuts at: 1068 3417 3673 1068
Size: 2349 256 1459
Fragments arranged by size:
2349 1459 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 1084 1084
Size: 4064
BamHI G'GATC_C
Cuts at: 1089 1089
Size: 4064
BanI G'GyrC_C
Cuts at: 1080 1494 1742 1777 1080
Size: 414 248 35 3367
Fragments arranged by size:
3367 414 248 35
BanII G_rGCy'C
Cuts at: 1078 2108 1078
Size: 1030 3034
BbeI G_GCGC'C
Cuts at: 1498 1746 1498
Size: 248 3816
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4030
BciVI GTATCCnnnnn_n'
Cuts at: 1962 2723 4064 1962
Size: 761 1341 1962
Fragments arranged by size:
1962 1341 761
BclI T'GATC_A
Cuts at: 1584 1584
Size: 4064
BglII A'GATC_T
Cuts at: 1579 3616 1579
Size: 2037 2027
BlpI GC'TnA_GC
Cuts at: 715 1176 715
Size: 461 3603
Bme1580I G_kGCm'C
Cuts at: 1689 1782 2838 1689
Size: 93 1056 2915
Fragments arranged by size:
2915 1056 93
BmgBI CAC'GTC
Cuts at: 186 186
Size: 4064
BmrI ACTGGGnnnn_n'
Cuts at: 1276 1357 1692 3836 1276
Size: 81 335 2144 1504
Fragments arranged by size:
2144 1504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 4064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 911 193
Size: 718 3346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 2119 2611 2909 1240
Size: 879 492 298 2395
Fragments arranged by size:
2395 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3768 3768
Size: 4064
BsaAI yAC'GTr
Cuts at: 483 1417 483
Size: 934 3130
BsaBI GATnn'nnATC
Cuts at: 6 1578 3615 6
Size: 1572 2037 455
Fragments arranged by size:
2037 1572 455
BsaHI Gr'CG_yC
Cuts at: 398 1495 1743 2445 3941 398
Size: 1097 248 702 1496 521
Fragments arranged by size:
1496 1097 702 521 248
BsaWI w'CCGG_w
Cuts at: 1253 1774 2452 2726 2873 3284 1253
Size: 521 678 274 147 411 2033
Fragments arranged by size:
2033 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 550 914 949 550
Size: 364 35 3665
Fragments arranged by size:
3665 364 35
BseYI C'CCAG_C
Cuts at: 139 1489 2247 2824 3320 139
Size: 1350 758 577 496 883
Fragments arranged by size:
1350 883 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 262
Size: 4064
BsiEI CG_ry'CG
Cuts at: 104 1606 1652 2860 104
Size: 1502 46 1208 1308
Fragments arranged by size:
1502 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 521 659 1078 1856 2046 2838 521
Size: 138 419 778 190 792 1747
Fragments arranged by size:
1747 792 778 419 190 138
Bsp1286I G_dGCh'C
Cuts at: 521 659 1078 1689 1782 1856 2046 2108 2838
Size: 138 419 611 93 74 190 62 730
Cuts at: 2838 521
Size: 1747
Fragments arranged by size:
1747 730 611 419 190 138 93 74
62
BspEI T'CCGG_A
Cuts at: 3284 3284
Size: 4064
BspHI T'CATG_A
Cuts at: 3240 3240
Size: 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1114 1328 1630 2011 1114
Size: 214 302 381 3167
Fragments arranged by size:
3167 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2356 2410 3894 4059 50
Size: 2306 54 1484 165 55
Fragments arranged by size:
2306 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 878 1976 878
Size: 1098 2966
BsrFI r'CCGG_y
Cuts at: 2062 2243 2452 2062
Size: 181 209 3674
Fragments arranged by size:
3674 209 181
BssSI C'ACGA_G
Cuts at: 2335 2693 2335
Size: 358 3706
BstAPI GCAn_nnn'nTGC
Cuts at: 1441 1441
Size: 4064
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstXI CCAn_nnnn'nTGG
Cuts at: 219 324 357 219
Size: 105 33 3926
Fragments arranged by size:
3926 105 33
BstYI r'GATC_y
Cuts at: 1 1010 1089 1556 1579 1914 2160 3172 3258
Size: 1009 79 467 23 335 246 1012 86
Cuts at: 3258 3270 3616 1
Size: 12 346 449
Fragments arranged by size:
1012 1009 467 449 346 335 246 86
79 23 12
Bsu36I CC'TnA_GG
Cuts at: 809 809
Size: 4064
BtgI C'CryG_G
Cuts at: 464 464
Size: 4064
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2014 2195 2358 2014
Size: 181 163 3720
Fragments arranged by size:
3720 181 163
BtsI GCAGTG_nn'
Cuts at: 426 1307 426
Size: 881 3183
DraI TTT'AAA
Cuts at: 1063 1063
Size: 4064
DrdI GACnn_nn'nnGTC
Cuts at: 694 1770 2628 694
Size: 1076 858 2130
Fragments arranged by size:
2130 1076 858
EaeI y'GGCC_r
Cuts at: 485 944 1649 1823 2214 2241 3586 485
Size: 459 705 174 391 27 1345 963
Fragments arranged by size:
1345 963 705 459 391 174 27
EagI C'GGCC_G
Cuts at: 1649 1649
Size: 4064
EarI CTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 592 2272 2582 2728 3874 592
Size: 1680 310 146 1146 782
Fragments arranged by size:
1680 1146 782 310 146
EcoICRI GAG'CTC
Cuts at: 1076 1076
Size: 4064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 911 1889 2321 3068 3336 193
Size: 718 978 432 747 268 921
Fragments arranged by size:
978 921 747 718 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1203 1203
Size: 4064
EcoRI G'AATT_C
Cuts at: 1068 1068
Size: 4064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 4064
FspI TGC'GCA
Cuts at: 1845 1845
Size: 4064
HaeII r_GCGC'y
Cuts at: 1498 1746 2768 3747 1498
Size: 248 1022 979 1815
Fragments arranged by size:
1815 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1906 1936 1938 1968 1906
Size: 30 2 30 4002
Fragments arranged by size:
4002 30 30 2
HincII GTy'rAC
Cuts at: 1103 1103
Size: 4064
KasI G'GCGC_C
Cuts at: 1494 1742 1494
Size: 248 3816
KpnI G_GTAC'C
Cuts at: 1084 1084
Size: 4064
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2735 2919 2735
Size: 184 3880
MscI TGG'CCA
Cuts at: 487 946 1825 487
Size: 459 879 2726
Fragments arranged by size:
2726 879 459
MslI CAynn'nnrTG
Cuts at: 851 2053 2180 851
Size: 1202 127 2735
Fragments arranged by size:
2735 1202 127
MspA1I CmG'CkG
Cuts at: 1175 1489 1849 2862 3107 1175
Size: 314 360 1013 245 2132
Fragments arranged by size:
2132 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2245 2245
Size: 4064
NarI GG'CG_CC
Cuts at: 1495 1743 1495
Size: 248 3816
NcoI C'CATG_G
Cuts at: 464 464
Size: 4064
NgoMIV G'CCGG_C
Cuts at: 2243 2243
Size: 4064
NspI r_CATG'y
Cuts at: 309 683 839 1117 1617 2524 309
Size: 374 156 278 500 907 1849
Fragments arranged by size:
1849 907 500 374 278 156
PasI CC'CwG_GG
Cuts at: 166 828 166
Size: 662 3402
PciI A'CATG_T
Cuts at: 679 2520 679
Size: 1841 2223
PfoI T'CCnGG_A
Cuts at: 740 740
Size: 4064
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 4032
PsiI TTA'TAA
Cuts at: 623 623
Size: 4064
PstI C_TGCA'G
Cuts at: 1111 1796 1111
Size: 685 3379
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 1489 1849 1489
Size: 360 3704
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 1078 1078
Size: 4064
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SexAI A'CCwGG_T
Cuts at: 218 323 218
Size: 105 3959
SfcI C'TryA_G
Cuts at: 33 665 1107 1792 2785 2976 33
Size: 632 442 685 993 191 1121
Fragments arranged by size:
1121 993 685 632 442 191
SfoI GGC'GCC
Cuts at: 1496 1744 1496
Size: 248 3816
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 1219 2134 2626 2888 1219
Size: 915 492 262 2395
Fragments arranged by size:
2395 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 1117 1617 1117
Size: 500 3564
StyI C'CwwG_G
Cuts at: 464 1036 1198 1552 464
Size: 572 162 354 2976
Fragments arranged by size:
2976 572 354 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1647 1780 2239 2448 1647
Size: 133 459 209 3263
Fragments arranged by size:
3263 459 209 133
TatI w'GTAC_w
Cuts at: 290 3579 290
Size: 3289 775
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 4032
Tth111I GACn'n_nGTC
Cuts at: 1861 1861
Size: 4064
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 219 252 1559 219
Size: 33 1307 2724
Fragments arranged by size:
2724 1307 33
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI