Restriction map for
pF1KB7382
(Circular) (Six-base) MAPSORT of: pF1KB7382.seq Check: 2837 from: 1 to: 4052
pF1KB7382 4052 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 531 603 820 1090 531
Size: 72 217 270 3493
Fragments arranged by size:
3493 270 217 72
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 818 1877 2309 3056 3324 818
Size: 1059 432 747 268 1546
Fragments arranged by size:
1546 1059 747 432 268
AflIII A'CryG_T
Cuts at: 1404 2508 3485 1404
Size: 1104 977 1971
Fragments arranged by size:
1971 1104 977
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AhdI GACnn_n'nnGTC
Cuts at: 599 599
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 1357 2924 1357
Size: 1567 2485
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 139 1056 3405 3661 139
Size: 917 2349 256 530
Fragments arranged by size:
2349 917 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 1072 1072
Size: 4052
BamHI G'GATC_C
Cuts at: 1077 1077
Size: 4052
BanI G'GyrC_C
Cuts at: 803 1068 1482 1730 1765 803
Size: 265 414 248 35 3090
Fragments arranged by size:
3090 414 265 248 35
BanII G_rGCy'C
Cuts at: 1066 2096 1066
Size: 1030 3022
BbeI G_GCGC'C
Cuts at: 807 1486 1734 807
Size: 679 248 3125
Fragments arranged by size:
3125 679 248
BbsI GAAGACnn'nnnn_
Cuts at: 138 918 138
Size: 780 3272
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 452 1572 452
Size: 1120 2932
BglII A'GATC_T
Cuts at: 440 1567 3604 440
Size: 1127 2037 888
Fragments arranged by size:
2037 1127 888
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 1677 1770 2826 1677
Size: 93 1056 2903
Fragments arranged by size:
2903 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
Bpu10I CC'TnA_GC
Cuts at: 295 1009 295
Size: 714 3338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 993 1228 2107 2599 2897 993
Size: 235 879 492 298 2148
Fragments arranged by size:
2148 879 492 298 235
BsaI GGTCTCn'nnnn_
Cuts at: 3756 3756
Size: 4052
BsaAI yAC'GTr
Cuts at: 477 1405 477
Size: 928 3124
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 804 1483 1731 2433 3929 804
Size: 679 248 702 1496 927
Fragments arranged by size:
1496 927 702 679 248
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 997 997
Size: 4052
BseYI C'CCAG_C
Cuts at: 1477 2235 2812 3308 1477
Size: 758 577 496 2221
Fragments arranged by size:
2221 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 471 657 471
Size: 186 3866
BsiEI CG_ry'CG
Cuts at: 104 1594 1640 2848 104
Size: 1490 46 1208 1308
Fragments arranged by size:
1490 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1066 1844 2034 2826 1066
Size: 778 190 792 2292
Fragments arranged by size:
2292 792 778 190
BsmI GAATG_Cn'
Cuts at: 196 196
Size: 4052
Bsp1286I G_dGCh'C
Cuts at: 1066 1677 1770 1844 2034 2096 2826 1066
Size: 611 93 74 190 62 730 2292
Fragments arranged by size:
2292 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 3228 3228
Size: 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 832 1102 1316 1618 1999 832
Size: 270 214 302 381 2885
Fragments arranged by size:
2885 381 302 270 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 245 1964 245
Size: 1719 2333
BsrFI r'CCGG_y
Cuts at: 2050 2231 2440 2050
Size: 181 209 3662
Fragments arranged by size:
3662 209 181
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 783 2413 783
Size: 1630 2422
BstEII G'GTnAC_C
Cuts at: 750 939 750
Size: 189 3863
BstYI r'GATC_y
Cuts at: 1 440 1077 1544 1567 1902 2148 3160 3246
Size: 439 637 467 23 335 246 1012 86
Cuts at: 3246 3258 3604 1
Size: 12 346 449
Fragments arranged by size:
1012 637 467 449 439 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 532 532
Size: 4052
BtgI C'CryG_G
Cuts at: 107 746 107
Size: 639 3413
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 1295 1295
Size: 4052
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 743 886 1637 1811 2202 2229 3574 743
Size: 143 751 174 391 27 1345 1221
Fragments arranged by size:
1345 1221 751 391 174 143 27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 415 785 859 2075 2285 415
Size: 370 74 1216 210 2182
Fragments arranged by size:
2182 1216 370 210 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1064 1064
Size: 4052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 818 1877 2309 3056 3324 818
Size: 1059 432 747 268 1546
Fragments arranged by size:
1546 1059 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1191 1191
Size: 4052
EcoRI G'AATT_C
Cuts at: 1056 1056
Size: 4052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 4052
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 807 1486 1734 2756 3735 807
Size: 679 248 1022 979 1124
Fragments arranged by size:
1124 1022 979 679 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 585 617 1894 1924 1926 1956 585
Size: 32 1277 30 2 30 2681
Fragments arranged by size:
2681 1277 32 30 30 2
HincII GTy'rAC
Cuts at: 1091 1091
Size: 4052
KasI G'GCGC_C
Cuts at: 803 1482 1730 803
Size: 679 248 3125
Fragments arranged by size:
3125 679 248
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 332 2723 2907 332
Size: 2391 184 1477
Fragments arranged by size:
2391 1477 184
MscI TGG'CCA
Cuts at: 1813 1813
Size: 4052
MslI CAynn'nnrTG
Cuts at: 211 2041 2168 211
Size: 1830 127 2095
Fragments arranged by size:
2095 1830 127
MspA1I CmG'CkG
Cuts at: 796 1163 1477 1837 2850 3095 796
Size: 367 314 360 1013 245 1753
Fragments arranged by size:
1753 1013 367 360 314 245
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 804 1483 1731 804
Size: 679 248 3125
Fragments arranged by size:
3125 679 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4052
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NspI r_CATG'y
Cuts at: 1105 1605 2512 1105
Size: 500 907 2645
Fragments arranged by size:
2645 907 500
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PflMI CCAn_nnn'nTGG
Cuts at: 840 840
Size: 4052
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PstI C_TGCA'G
Cuts at: 1099 1784 1099
Size: 685 3367
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 796 1477 1837 796
Size: 681 360 3011
Fragments arranged by size:
3011 681 360
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 1066 1066
Size: 4052
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 415 2075 2285 415
Size: 1660 210 2182
Fragments arranged by size:
2182 1660 210
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SfcI C'TryA_G
Cuts at: 33 1095 1780 2773 2964 33
Size: 1062 685 993 191 1121
Fragments arranged by size:
1121 1062 993 685 191
SfoI GGC'GCC
Cuts at: 805 1484 1732 805
Size: 679 248 3125
Fragments arranged by size:
3125 679 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 972 1207 2122 2614 2876 972
Size: 235 915 492 262 2148
Fragments arranged by size:
2148 915 492 262 235
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
StyI C'CwwG_G
Cuts at: 107 1186 1540 107
Size: 1079 354 2619
Fragments arranged by size:
2619 1079 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1635 1768 2227 2436 1635
Size: 133 459 209 3251
Fragments arranged by size:
3251 459 209 133
TatI w'GTAC_w
Cuts at: 3567 3567
Size: 4052
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 912 1547 912
Size: 635 3417
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
XmnI GAAnn'nnTTC
Cuts at: 958 958
Size: 4052
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BsiWI BsmBI BsrGI BssHII BstXI
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI