Restriction map for
pF1KB7378
(Circular) (Six-base) MAPSORT of: pF1KB7378.seq Check: 444 from: 1 to: 3947
pF1KB7378 3947 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 542 609 542
Size: 67 3880
AccI GT'mk_AC
Cuts at: 985 985
Size: 3947
Acc65I G'GTAC_C
Cuts at: 963 963
Size: 3947
AclI AA'CG_TT
Cuts at: 3787 3787
Size: 3947
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1772 2204 2951 3219 1772
Size: 432 747 268 2500
Fragments arranged by size:
2500 747 432 268
AflIII A'CryG_T
Cuts at: 1299 2403 3380 1299
Size: 1104 977 1866
Fragments arranged by size:
1866 1104 977
AgeI A'CCGG_T
Cuts at: 2335 2335
Size: 3947
AlwNI CAG_nnn'CTG
Cuts at: 482 1252 2819 482
Size: 770 1567 1610
Fragments arranged by size:
1610 1567 770
ApaI G_GGCC'C
Cuts at: 763 763
Size: 3947
ApaLI G'TGCA_C
Cuts at: 2717 2717
Size: 3947
ApoI r'AATT_y
Cuts at: 951 3300 3556 951
Size: 2349 256 1342
Fragments arranged by size:
2349 1342 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3947
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3947
AvaI C'yCGr_G
Cuts at: 177 271 406 967 177
Size: 94 135 561 3157
Fragments arranged by size:
3157 561 135 94
BamHI G'GATC_C
Cuts at: 188 972 188
Size: 784 3163
BanI G'GyrC_C
Cuts at: 209 249 267 412 442 697 963 1377 1625
Size: 40 18 145 30 255 266 414 248
Cuts at: 1625 1660 209
Size: 35 2496
Fragments arranged by size:
2496 414 266 255 248 145 40 35
30 18
BanII G_rGCy'C
Cuts at: 422 763 961 1991 422
Size: 341 198 1030 2378
Fragments arranged by size:
2378 1030 341 198
BbeI G_GCGC'C
Cuts at: 213 253 271 1381 1629 213
Size: 40 18 1110 248 2531
Fragments arranged by size:
2531 1110 248 40 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3913
BciVI GTATCCnnnnn_n'
Cuts at: 1845 2606 3947 1845
Size: 761 1341 1845
Fragments arranged by size:
1845 1341 761
BclI T'GATC_A
Cuts at: 794 1467 794
Size: 673 3274
BglI GCCn_nnn'nGGC
Cuts at: 350 397 350
Size: 47 3900
BglII A'GATC_T
Cuts at: 891 1462 3499 891
Size: 571 2037 1339
Fragments arranged by size:
2037 1339 571
BlpI GC'TnA_GC
Cuts at: 1059 1059
Size: 3947
Bme1580I G_kGCm'C
Cuts at: 415 700 763 1572 1665 2721 415
Size: 285 63 809 93 1056 1641
Fragments arranged by size:
1641 1056 809 285 93 63
BmgBI CAC'GTC
Cuts at: 198 198
Size: 3947
BmrI ACTGGGnnnn_n'
Cuts at: 652 1159 1240 1575 3719 652
Size: 507 81 335 2144 880
Fragments arranged by size:
2144 880 507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 3947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 730
Size: 3947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1123 2002 2494 2792 1123
Size: 879 492 298 2278
Fragments arranged by size:
2278 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3651 3651
Size: 3947
BsaAI yAC'GTr
Cuts at: 668 1300 668
Size: 632 3315
BsaBI GATnn'nnATC
Cuts at: 6 793 1461 3498 6
Size: 787 668 2037 455
Fragments arranged by size:
2037 787 668 455
BsaHI Gr'CG_yC
Cuts at: 210 250 268 1378 1626 2328 3824 210
Size: 40 18 1110 248 702 1496 333
Fragments arranged by size:
1496 1110 702 333 248 40 18
BsaWI w'CCGG_w
Cuts at: 1136 1657 2335 2609 2756 3167 1136
Size: 521 678 274 147 411 1916
Fragments arranged by size:
1916 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 727 727
Size: 3947
BseYI C'CCAG_C
Cuts at: 394 475 921 1372 2130 2707 3203 394
Size: 81 446 451 758 577 496 1138
Fragments arranged by size:
1138 758 577 496 451 446 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 520
Size: 3947
BsiEI CG_ry'CG
Cuts at: 104 207 465 1489 1535 2743 104
Size: 103 258 1024 46 1208 1308
Fragments arranged by size:
1308 1208 1024 258 103 46
BsiHKAI G_wGCw'C
Cuts at: 961 1739 1929 2721 961
Size: 778 190 792 2187
Fragments arranged by size:
2187 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 415 422 700 763 961 1572 1665 1739 1929
Size: 7 278 63 198 611 93 74 190
Cuts at: 1929 1991 2721 415
Size: 62 730 1641
Fragments arranged by size:
1641 730 611 278 198 190 93 74
63 62 7
BspEI T'CCGG_A
Cuts at: 3167 3167
Size: 3947
BspHI T'CATG_A
Cuts at: 3123 3123
Size: 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 542 609 997 1211 1513 1894 542
Size: 67 388 214 302 381 2595
Fragments arranged by size:
2595 388 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2239 2293 3777 3942 50
Size: 2189 54 1484 165 55
Fragments arranged by size:
2189 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1859 1859
Size: 3947
BsrFI r'CCGG_y
Cuts at: 206 230 349 1945 2126 2335 206
Size: 24 119 1596 181 209 1818
Fragments arranged by size:
1818 1596 209 181 119 24
BssHII G'CGCG_C
Cuts at: 431 485 498 518 544 431
Size: 54 13 20 26 3834
Fragments arranged by size:
3834 54 26 20 13
BssSI C'ACGA_G
Cuts at: 2218 2576 2218
Size: 358 3589
BstAPI GCAn_nnn'nTGC
Cuts at: 449 1324 449
Size: 875 3072
BstBI TT'CG_AA
Cuts at: 2308 2308
Size: 3947
BstEII G'GTnAC_C
Cuts at: 529 529
Size: 3947
BstXI CCAn_nnnn'nTGG
Cuts at: 394 852 394
Size: 458 3489
BstYI r'GATC_y
Cuts at: 1 188 275 891 972 1439 1462 1797 2043
Size: 187 87 616 81 467 23 335 246
Cuts at: 2043 3055 3141 3153 3499 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 616 467 449 346 335 246 187
87 86 81 23 12
Bsu36I CC'TnA_GG
Cuts at: 692 692
Size: 3947
BtgI C'CryG_G
Cuts at: 779 779
Size: 3947
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1897 2078 2241 1897
Size: 181 163 3603
Fragments arranged by size:
3603 181 163
BtsI GCAGTG_nn'
Cuts at: 1190 1190
Size: 3947
DraI TTT'AAA
Cuts at: 946 946
Size: 3947
DrdI GACnn_nn'nnGTC
Cuts at: 1653 2511 1653
Size: 858 3089
EaeI y'GGCC_r
Cuts at: 204 232 1532 1706 2097 2124 3469 204
Size: 28 1300 174 391 27 1345 682
Fragments arranged by size:
1345 1300 682 391 174 28 27
EagI C'GGCC_G
Cuts at: 204 1532 204
Size: 1328 2619
EarI CTCTTCn'nnn_
Cuts at: 1970 2180 1970
Size: 210 3737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2155 2465 2611 3757 2155
Size: 310 146 1146 2345
Fragments arranged by size:
2345 1146 310 146
EcoICRI GAG'CTC
Cuts at: 959 959
Size: 3947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1772 2204 2951 3219 730
Size: 1042 432 747 268 1458
Fragments arranged by size:
1458 1042 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 538 808 538
Size: 270 3677
EcoO109I rG'GnC_Cy
Cuts at: 759 760 1086 759
Size: 1 326 3620
Fragments arranged by size:
3620 326 1
EcoRI G'AATT_C
Cuts at: 951 951
Size: 3947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1966 1966
Size: 3947
FspI TGC'GCA
Cuts at: 1728 1728
Size: 3947
HaeII r_GCGC'y
Cuts at: 213 253 271 286 360 406 1381 1629 2651
Size: 40 18 15 74 46 975 248 1022
Cuts at: 2651 3630 213
Size: 979 530
Fragments arranged by size:
1022 979 975 530 248 74 46 40
18 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 850 882 1789 1819 1821 1851 451
Size: 32 367 32 907 30 2 30 2547
Fragments arranged by size:
2547 907 367 32 32 30 30 2
HincII GTy'rAC
Cuts at: 986 986
Size: 3947
KasI G'GCGC_C
Cuts at: 209 249 267 1377 1625 209
Size: 40 18 1110 248 2531
Fragments arranged by size:
2531 1110 248 40 18
KpnI G_GTAC'C
Cuts at: 967 967
Size: 3947
MluI A'CGCG_T
Cuts at: 3380 3380
Size: 3947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 482 2618 2802 482
Size: 2136 184 1627
Fragments arranged by size:
2136 1627 184
MscI TGG'CCA
Cuts at: 1708 1708
Size: 3947
MslI CAynn'nnrTG
Cuts at: 1936 2063 1936
Size: 127 3820
MspA1I CmG'CkG
Cuts at: 135 159 171 311 394 1058 1372 1732 2745
Size: 24 12 140 83 664 314 360 1013
Cuts at: 2745 2990 135
Size: 245 1092
Fragments arranged by size:
1092 1013 664 360 314 245 140 83
24 12
NaeI GCC'GGC
Cuts at: 208 232 351 2128 208
Size: 24 119 1777 2027
Fragments arranged by size:
2027 1777 119 24
NarI GG'CG_CC
Cuts at: 210 250 268 1378 1626 210
Size: 40 18 1110 248 2531
Fragments arranged by size:
2531 1110 248 40 18
NgoMIV G'CCGG_C
Cuts at: 206 230 349 2126 206
Size: 24 119 1777 2027
Fragments arranged by size:
2027 1777 119 24
NspI r_CATG'y
Cuts at: 1000 1500 2407 1000
Size: 500 907 2540
Fragments arranged by size:
2540 907 500
PciI A'CATG_T
Cuts at: 2403 2403
Size: 3947
PflMI CCAn_nnn'nTGG
Cuts at: 708 785 708
Size: 77 3870
PmeI GTTT'AAAC
Cuts at: 946 946
Size: 3947
PmlI CAC'GTG
Cuts at: 668 668
Size: 3947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 3915
PspOMI G'GGCC_C
Cuts at: 759 759
Size: 3947
PstI C_TGCA'G
Cuts at: 994 1679 994
Size: 685 3262
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3947
PvuII CAG'CTG
Cuts at: 1372 1732 1372
Size: 360 3587
RsrII CG'GwC_CG
Cuts at: 2142 2142
Size: 3947
SacI G_AGCT'C
Cuts at: 961 961
Size: 3947
SalI G'TCGA_C
Cuts at: 984 984
Size: 3947
SapI GCTCTTCn'nnn_
Cuts at: 1970 2180 1970
Size: 210 3737
SbfI CC_TGCA'GG
Cuts at: 994 994
Size: 3947
ScaI AGT'ACT
Cuts at: 3464 3464
Size: 3947
SfcI C'TryA_G
Cuts at: 33 990 1675 2668 2859 33
Size: 957 685 993 191 1121
Fragments arranged by size:
1121 993 957 685 191
SfoI GGC'GCC
Cuts at: 211 251 269 1379 1627 211
Size: 40 18 1110 248 2531
Fragments arranged by size:
2531 1110 248 40 18
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3947
SgrAI Cr'CCGG_yG
Cuts at: 349 349
Size: 3947
SmaI CCC'GGG
Cuts at: 273 969 273
Size: 696 3251
SmlI C'TyrA_G
Cuts at: 1102 2017 2509 2771 1102
Size: 915 492 262 2278
Fragments arranged by size:
2278 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3947
SphI G_CATG'C
Cuts at: 1000 1500 1000
Size: 500 3447
StyI C'CwwG_G
Cuts at: 183 1081 1435 183
Size: 898 354 2695
Fragments arranged by size:
2695 898 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 715 1530 1663 2122 2331 715
Size: 815 133 459 209 2331
Fragments arranged by size:
2331 815 459 209 133
TatI w'GTAC_w
Cuts at: 3462 3462
Size: 3947
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 3915
Tth111I GACn'n_nGTC
Cuts at: 730 1744 730
Size: 1014 2933
XbaI T'CTAG_A
Cuts at: 888 978 888
Size: 90 3857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 665 1442 665
Size: 777 3170
XmaI C'CCGG_G
Cuts at: 271 967 271
Size: 696 3251
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmeI
PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI