Restriction map for
pF1KB7377
(Circular) (Six-base) MAPSORT of: pF1KB7377.seq Check: 2719 from: 1 to: 4460
pF1KB7377 4460 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 361 798 1133 361
Size: 437 335 3688
Fragments arranged by size:
3688 437 335
AccI GT'mk_AC
Cuts at: 887 1498 887
Size: 611 3849
Acc65I G'GTAC_C
Cuts at: 1476 1476
Size: 4460
AclI AA'CG_TT
Cuts at: 4300 4300
Size: 4460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 2717 3464 3732 2285
Size: 432 747 268 3013
Fragments arranged by size:
3013 747 432 268
AflIII A'CryG_T
Cuts at: 1812 2916 3893 1812
Size: 1104 977 2379
Fragments arranged by size:
2379 1104 977
AgeI A'CCGG_T
Cuts at: 2848 2848
Size: 4460
AleI CACnn'nnGTG
Cuts at: 758 784 758
Size: 26 4434
AlwNI CAG_nnn'CTG
Cuts at: 1161 1765 3332 1161
Size: 604 1567 2289
Fragments arranged by size:
2289 1567 604
ApaI G_GGCC'C
Cuts at: 160 160
Size: 4460
ApaLI G'TGCA_C
Cuts at: 699 3230 699
Size: 2531 1929
ApoI r'AATT_y
Cuts at: 1464 3813 4069 1464
Size: 2349 256 1855
Fragments arranged by size:
2349 1855 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4460
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
AvaI C'yCGr_G
Cuts at: 978 1383 1480 978
Size: 405 97 3958
Fragments arranged by size:
3958 405 97
BamHI G'GATC_C
Cuts at: 301 1485 301
Size: 1184 3276
BanI G'GyrC_C
Cuts at: 432 749 1092 1476 1890 2138 2173 432
Size: 317 343 384 414 248 35 2719
Fragments arranged by size:
2719 414 384 343 317 248 35
BanII G_rGCy'C
Cuts at: 160 167 1028 1474 2504 160
Size: 7 861 446 1030 2116
Fragments arranged by size:
2116 1030 861 446 7
BbeI G_GCGC'C
Cuts at: 1894 2142 1894
Size: 248 4212
BbsI GAAGACnn'nnnn_
Cuts at: 807 1333 807
Size: 526 3934
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4426
BciVI GTATCCnnnnn_n'
Cuts at: 599 2358 3119 4460 599
Size: 1759 761 1341 599
Fragments arranged by size:
1759 1341 761 599
BclI T'GATC_A
Cuts at: 1980 1980
Size: 4460
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4460
BglII A'GATC_T
Cuts at: 374 1975 4012 374
Size: 1601 2037 822
Fragments arranged by size:
2037 1601 822
BlpI GC'TnA_GC
Cuts at: 1572 1572
Size: 4460
Bme1580I G_kGCm'C
Cuts at: 160 437 703 752 2085 2178 3234 160
Size: 277 266 49 1333 93 1056 1386
Fragments arranged by size:
1386 1333 1056 277 266 93 49
BmgBI CAC'GTC
Cuts at: 654 654
Size: 4460
BmrI ACTGGGnnnn_n'
Cuts at: 633 1672 1753 2088 4232 633
Size: 1039 81 335 2144 861
Fragments arranged by size:
2144 1039 861 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 321 2827 321
Size: 2506 1954
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 216 391 897 1039 1330 151
Size: 65 175 506 142 291 3281
Fragments arranged by size:
3281 506 291 175 142 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1128 1636 2515 3007 3305 1128
Size: 508 879 492 298 2283
Fragments arranged by size:
2283 879 508 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4164 4164
Size: 4460
BsaAI yAC'GTr
Cuts at: 714 1813 714
Size: 1099 3361
BsaBI GATnn'nnATC
Cuts at: 6 1974 4011 6
Size: 1968 2037 455
Fragments arranged by size:
2037 1968 455
BsaHI Gr'CG_yC
Cuts at: 548 1891 2139 2841 4337 548
Size: 1343 248 702 1496 671
Fragments arranged by size:
1496 1343 702 671 248
BsaWI w'CCGG_w
Cuts at: 1649 2170 2848 3122 3269 3680 1649
Size: 521 678 274 147 411 2429
Fragments arranged by size:
2429 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 916 946 916
Size: 30 4430
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 444 1036 1102 444
Size: 592 66 3802
Fragments arranged by size:
3802 592 66
BseYI C'CCAG_C
Cuts at: 153 411 928 1101 1230 1447 1885 2643 3220
Size: 258 517 173 129 217 438 758 577
Cuts at: 3220 3716 153
Size: 496 897
Fragments arranged by size:
897 758 577 517 496 438 258 217
173 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 1156 294
Size: 862 3598
BsiEI CG_ry'CG
Cuts at: 104 2002 2048 3256 104
Size: 1898 46 1208 1308
Fragments arranged by size:
1898 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 209 703 1474 2252 2442 3234 209
Size: 494 771 778 190 792 1435
Fragments arranged by size:
1435 792 778 771 494 190
BsmI GAATG_Cn'
Cuts at: 880 880
Size: 4460
Bsp1286I G_dGCh'C
Cuts at: 160 167 209 437 703 752 1028 1474 2085
Size: 7 42 228 266 49 276 446 611
Cuts at: 2085 2178 2252 2442 2504 3234 160
Size: 93 74 190 62 730 1386
Fragments arranged by size:
1386 730 611 446 276 266 228 190
93 74 62 49 42 7
BspEI T'CCGG_A
Cuts at: 3680 3680
Size: 4460
BspHI T'CATG_A
Cuts at: 3636 3636
Size: 4460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 361 416 798 1133 1510 1724 2026 2407 361
Size: 55 382 335 377 214 302 381 2414
Fragments arranged by size:
2414 382 381 377 335 302 214 55
BsrBI CCG'CTC
Cuts at: 50 2752 2806 4290 4455 50
Size: 2702 54 1484 165 55
Fragments arranged by size:
2702 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 696 2372 696
Size: 1676 2784
BsrFI r'CCGG_y
Cuts at: 2458 2639 2848 2458
Size: 181 209 4070
Fragments arranged by size:
4070 209 181
BssSI C'ACGA_G
Cuts at: 784 2731 3089 784
Size: 1947 358 2155
Fragments arranged by size:
2155 1947 358
BstAPI GCAn_nnn'nTGC
Cuts at: 617 1837 617
Size: 1220 3240
BstBI TT'CG_AA
Cuts at: 2821 2821
Size: 4460
BstXI CCAn_nnnn'nTGG
Cuts at: 760 760
Size: 4460
BstYI r'GATC_y
Cuts at: 1 301 374 1485 1952 1975 2310 2556 3568
Size: 300 73 1111 467 23 335 246 1012
Cuts at: 3568 3654 3666 4012 1
Size: 86 12 346 449
Fragments arranged by size:
1111 1012 467 449 346 335 300 246
86 73 23 12
BstZ17I GTA'TAC
Cuts at: 888 888
Size: 4460
BtgI C'CryG_G
Cuts at: 179 507 744 843 179
Size: 328 237 99 3796
Fragments arranged by size:
3796 328 237 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2410 2591 2754 2410
Size: 181 163 4116
Fragments arranged by size:
4116 181 163
BtsI GCAGTG_nn'
Cuts at: 148 149 1703 148
Size: 1 1554 2905
Fragments arranged by size:
2905 1554 1
DraI TTT'AAA
Cuts at: 1459 1459
Size: 4460
DraIII CAC_nnn'GTG
Cuts at: 272 627 272
Size: 355 4105
DrdI GACnn_nn'nnGTC
Cuts at: 561 2166 3024 561
Size: 1605 858 1997
Fragments arranged by size:
1997 1605 858
EaeI y'GGCC_r
Cuts at: 293 500 893 2045 2219 2610 2637 3982 293
Size: 207 393 1152 174 391 27 1345 771
Fragments arranged by size:
1345 1152 771 393 391 207 174 27
EagI C'GGCC_G
Cuts at: 2045 2045
Size: 4460
EarI CTCTTCn'nnn_
Cuts at: 689 2483 2693 689
Size: 1794 210 2456
Fragments arranged by size:
2456 1794 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 958 2668 2978 3124 4270 958
Size: 1710 310 146 1146 1148
Fragments arranged by size:
1710 1148 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1472 1472
Size: 4460
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 216 391 897 1039 1330 2285 2717 3464
Size: 65 175 506 142 291 955 432 747
Cuts at: 3464 3732 151
Size: 268 879
Fragments arranged by size:
955 879 747 506 432 291 268 175
142 65
EcoO109I rG'GnC_Cy
Cuts at: 386 570 730 773 1387 1599 386
Size: 184 160 43 614 212 3247
Fragments arranged by size:
3247 614 212 184 160 43
EcoRI G'AATT_C
Cuts at: 1464 1464
Size: 4460
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 843 1017 2479 843
Size: 174 1462 2824
Fragments arranged by size:
2824 1462 174
FspI TGC'GCA
Cuts at: 2241 2241
Size: 4460
HaeII r_GCGC'y
Cuts at: 1894 2142 3164 4143 1894
Size: 248 1022 979 2211
Fragments arranged by size:
2211 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2302 2332 2334 2364 2302
Size: 30 2 30 4398
Fragments arranged by size:
4398 30 30 2
HincII GTy'rAC
Cuts at: 1499 1499
Size: 4460
KasI G'GCGC_C
Cuts at: 1890 2138 1890
Size: 248 4212
KpnI G_GTAC'C
Cuts at: 1480 1480
Size: 4460
MluI A'CGCG_T
Cuts at: 3893 3893
Size: 4460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 848 3131 3315 848
Size: 2283 184 1993
Fragments arranged by size:
2283 1993 184
MscI TGG'CCA
Cuts at: 502 895 2221 502
Size: 393 1326 2741
Fragments arranged by size:
2741 1326 393
MslI CAynn'nnrTG
Cuts at: 758 784 895 2449 2576 758
Size: 26 111 1554 127 2642
Fragments arranged by size:
2642 1554 127 111 26
MspA1I CmG'CkG
Cuts at: 278 640 953 1230 1571 1885 2245 3258 3503
Size: 362 313 277 341 314 360 1013 245
Cuts at: 3503 278
Size: 1235
Fragments arranged by size:
1235 1013 362 360 341 314 313 277
245
NaeI GCC'GGC
Cuts at: 2641 2641
Size: 4460
NarI GG'CG_CC
Cuts at: 1891 2139 1891
Size: 248 4212
NcoI C'CATG_G
Cuts at: 179 507 179
Size: 328 4132
NgoMIV G'CCGG_C
Cuts at: 2639 2639
Size: 4460
NspI r_CATG'y
Cuts at: 466 499 1513 2013 2920 466
Size: 33 1014 500 907 2006
Fragments arranged by size:
2006 1014 907 500 33
PasI CC'CwG_GG
Cuts at: 160 160
Size: 4460
PciI A'CATG_T
Cuts at: 2916 2916
Size: 4460
PflMI CCAn_nnn'nTGG
Cuts at: 180 180
Size: 4460
PfoI T'CCnGG_A
Cuts at: 1004 1004
Size: 4460
PmeI GTTT'AAAC
Cuts at: 1459 1459
Size: 4460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4428
PpuMI rG'GwC_Cy
Cuts at: 570 1387 570
Size: 817 3643
PspOMI G'GGCC_C
Cuts at: 156 156
Size: 4460
PstI C_TGCA'G
Cuts at: 358 809 1507 2192 358
Size: 451 698 685 2626
Fragments arranged by size:
2626 698 685 451
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4460
PvuII CAG'CTG
Cuts at: 278 1230 1885 2245 278
Size: 952 655 360 2493
Fragments arranged by size:
2493 952 655 360
RsrII CG'GwC_CG
Cuts at: 2655 2655
Size: 4460
SacI G_AGCT'C
Cuts at: 1474 1474
Size: 4460
SalI G'TCGA_C
Cuts at: 1497 1497
Size: 4460
SanDI GG'GwC_CC
Cuts at: 1387 1387
Size: 4460
SapI GCTCTTCn'nnn_
Cuts at: 2483 2693 2483
Size: 210 4250
SbfI CC_TGCA'GG
Cuts at: 1507 1507
Size: 4460
ScaI AGT'ACT
Cuts at: 3977 3977
Size: 4460
SexAI A'CCwGG_T
Cuts at: 986 986
Size: 4460
SfcI C'TryA_G
Cuts at: 33 354 805 1503 2188 3181 3372 33
Size: 321 451 698 685 993 191 1121
Fragments arranged by size:
1121 993 698 685 451 321 191
SfoI GGC'GCC
Cuts at: 1892 2140 1892
Size: 248 4212
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
SmaI CCC'GGG
Cuts at: 980 1482 980
Size: 502 3958
SmlI C'TyrA_G
Cuts at: 1143 1615 2530 3022 3284 1143
Size: 472 915 492 262 2319
Fragments arranged by size:
2319 915 492 472 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4460
SphI G_CATG'C
Cuts at: 1513 2013 1513
Size: 500 3960
StuI AGG'CCT
Cuts at: 856 856
Size: 4460
StyI C'CwwG_G
Cuts at: 179 507 1594 1948 179
Size: 328 1087 354 2691
Fragments arranged by size:
2691 1087 354 328
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 615 2043 2176 2635 2844 615
Size: 1428 133 459 209 2231
Fragments arranged by size:
2231 1428 459 209 133
TatI w'GTAC_w
Cuts at: 3975 3975
Size: 4460
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 293 325 1098 1130 3977 4009 293
Size: 32 773 32 2847 32 744
Fragments arranged by size:
2847 773 744 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2257 2257
Size: 4460
XbaI T'CTAG_A
Cuts at: 1491 1491
Size: 4460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1955 1955
Size: 4460
XmaI C'CCGG_G
Cuts at: 978 1480 978
Size: 502 3958
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI
BssHII BstEII Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI