Restriction map for
pF1KB7376
(Circular) (Six-base) MAPSORT of: pF1KB7376.seq Check: 2389 from: 1 to: 4103
pF1KB7376 4103 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1141 1141
Size: 4103
Acc65I G'GTAC_C
Cuts at: 1119 1119
Size: 4103
AclI AA'CG_TT
Cuts at: 3943 3943
Size: 4103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 451 1928 2360 3107 3375 206
Size: 245 1477 432 747 268 934
Fragments arranged by size:
1477 934 747 432 268 245
AfeI AGC'GCT
Cuts at: 308 308
Size: 4103
AflIII A'CryG_T
Cuts at: 1455 2559 3536 1455
Size: 1104 977 2022
Fragments arranged by size:
2022 1104 977
AgeI A'CCGG_T
Cuts at: 2491 2491
Size: 4103
AlwNI CAG_nnn'CTG
Cuts at: 375 1408 2975 375
Size: 1033 1567 1503
Fragments arranged by size:
1567 1503 1033
ApaLI G'TGCA_C
Cuts at: 1068 2873 1068
Size: 1805 2298
ApoI r'AATT_y
Cuts at: 1107 3456 3712 1107
Size: 2349 256 1498
Fragments arranged by size:
2349 1498 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 832 1123 832
Size: 291 3812
BamHI G'GATC_C
Cuts at: 1128 1128
Size: 4103
BanI G'GyrC_C
Cuts at: 1119 1533 1781 1816 1119
Size: 414 248 35 3406
Fragments arranged by size:
3406 414 248 35
BanII G_rGCy'C
Cuts at: 1117 2147 1117
Size: 1030 3073
BbeI G_GCGC'C
Cuts at: 1537 1785 1537
Size: 248 3855
BbsI GAAGACnn'nnnn_
Cuts at: 334 334
Size: 4103
BciVI GTATCCnnnnn_n'
Cuts at: 2001 2762 4103 2001
Size: 761 1341 2001
Fragments arranged by size:
2001 1341 761
BclI T'GATC_A
Cuts at: 1055 1623 1055
Size: 568 3535
BglI GCCn_nnn'nGGC
Cuts at: 301 301
Size: 4103
BglII A'GATC_T
Cuts at: 1618 3655 1618
Size: 2037 2066
BlpI GC'TnA_GC
Cuts at: 1215 1215
Size: 4103
Bme1580I G_kGCm'C
Cuts at: 1072 1728 1821 2877 1072
Size: 656 93 1056 2298
Fragments arranged by size:
2298 1056 656 93
BmrI ACTGGGnnnn_n'
Cuts at: 1315 1396 1731 3875 1315
Size: 81 335 2144 1543
Fragments arranged by size:
2144 1543 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 894 427
Size: 467 3636
Bpu10I CC'TnA_GC
Cuts at: 997 997
Size: 4103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 998 1279 2158 2650 2948 268
Size: 730 281 879 492 298 1423
Fragments arranged by size:
1423 879 730 492 298 281
BsaI GGTCTCn'nnnn_
Cuts at: 828 1069 3807 828
Size: 241 2738 1124
Fragments arranged by size:
2738 1124 241
BsaAI yAC'GTr
Cuts at: 1456 1456
Size: 4103
BsaBI GATnn'nnATC
Cuts at: 6 1617 3654 6
Size: 1611 2037 455
Fragments arranged by size:
2037 1611 455
BsaHI Gr'CG_yC
Cuts at: 1534 1782 2484 3980 1534
Size: 248 702 1496 1657
Fragments arranged by size:
1657 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1292 1813 2491 2765 2912 3323 1292
Size: 521 678 274 147 411 2072
Fragments arranged by size:
2072 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 496 691 850 964 496
Size: 195 159 114 3635
Fragments arranged by size:
3635 195 159 114
BseYI C'CCAG_C
Cuts at: 221 1528 2286 2863 3359 221
Size: 1307 758 577 496 965
Fragments arranged by size:
1307 965 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1645 1691 2899 104
Size: 1541 46 1208 1308
Fragments arranged by size:
1541 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1072 1117 1895 2085 2877 1072
Size: 45 778 190 792 2298
Fragments arranged by size:
2298 792 778 190 45
BsmBI CGTCTCn'nnnn_
Cuts at: 261 642 261
Size: 381 3722
Bsp1286I G_dGCh'C
Cuts at: 1072 1117 1728 1821 1895 2085 2147 2877 1072
Size: 45 611 93 74 190 62 730 2298
Fragments arranged by size:
2298 730 611 190 93 74 62 45
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 4103
BspHI T'CATG_A
Cuts at: 3279 3279
Size: 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 360 1153 1367 1669 2050 360
Size: 793 214 302 381 2413
Fragments arranged by size:
2413 793 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2395 2449 3933 4098 50
Size: 2345 54 1484 165 55
Fragments arranged by size:
2345 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2015 2015
Size: 4103
BsrFI r'CCGG_y
Cuts at: 756 2101 2282 2491 756
Size: 1345 181 209 2368
Fragments arranged by size:
2368 1345 209 181
BssSI C'ACGA_G
Cuts at: 2374 2732 2374
Size: 358 3745
BstAPI GCAn_nnn'nTGC
Cuts at: 1480 1480
Size: 4103
BstBI TT'CG_AA
Cuts at: 2464 2464
Size: 4103
BstYI r'GATC_y
Cuts at: 1 131 839 1128 1595 1618 1953 2199 3211
Size: 130 708 289 467 23 335 246 1012
Cuts at: 3211 3297 3309 3655 1
Size: 86 12 346 449
Fragments arranged by size:
1012 708 467 449 346 335 289 246
130 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 322 322
Size: 4103
BtgI C'CryG_G
Cuts at: 107 641 720 107
Size: 534 79 3490
Fragments arranged by size:
3490 534 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2053 2234 2397 2053
Size: 181 163 3759
Fragments arranged by size:
3759 181 163
BtsI GCAGTG_nn'
Cuts at: 1072 1346 1072
Size: 274 3829
DraI TTT'AAA
Cuts at: 990 1102 990
Size: 112 3991
DrdI GACnn_nn'nnGTC
Cuts at: 1809 2667 1809
Size: 858 3245
EaeI y'GGCC_r
Cuts at: 644 1688 1862 2253 2280 3625 644
Size: 1044 174 391 27 1345 1122
Fragments arranged by size:
1345 1122 1044 391 174 27
EagI C'GGCC_G
Cuts at: 1688 1688
Size: 4103
EarI CTCTTCn'nnn_
Cuts at: 316 1043 2126 2336 316
Size: 727 1083 210 2083
Fragments arranged by size:
2083 1083 727 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 754 2311 2621 2767 3913 754
Size: 1557 310 146 1146 944
Fragments arranged by size:
1557 1146 944 310 146
EcoICRI GAG'CTC
Cuts at: 1115 1115
Size: 4103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 427 451 894 1928 2360 3107 3375 206
Size: 221 24 443 1034 432 747 268 934
Fragments arranged by size:
1034 934 747 443 432 268 221 24
EcoNI CCTnn'n_nnAGG
Cuts at: 673 697 739 847 673
Size: 24 42 108 3929
Fragments arranged by size:
3929 108 42 24
EcoO109I rG'GnC_Cy
Cuts at: 1242 1242
Size: 4103
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 153 2122 153
Size: 1969 2134
FspI TGC'GCA
Cuts at: 1884 1884
Size: 4103
HaeII r_GCGC'y
Cuts at: 310 628 1537 1785 2807 3786 310
Size: 318 909 248 1022 979 627
Fragments arranged by size:
1022 979 909 627 318 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1945 1975 1977 2007 1945
Size: 30 2 30 4041
Fragments arranged by size:
4041 30 30 2
HincII GTy'rAC
Cuts at: 1142 1142
Size: 4103
HindIII A'AGCT_T
Cuts at: 870 870
Size: 4103
KasI G'GCGC_C
Cuts at: 1533 1781 1533
Size: 248 3855
KpnI G_GTAC'C
Cuts at: 1123 1123
Size: 4103
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2774 2958 2774
Size: 184 3919
MscI TGG'CCA
Cuts at: 1864 1864
Size: 4103
MslI CAynn'nnrTG
Cuts at: 2092 2219 2092
Size: 127 3976
MspA1I CmG'CkG
Cuts at: 225 237 599 643 722 788 812 929 1214
Size: 12 362 44 79 66 24 117 285
Cuts at: 1214 1528 1888 2901 3146 225
Size: 314 360 1013 245 1182
Fragments arranged by size:
1182 1013 362 360 314 285 245 117
79 66 44 24 12
NaeI GCC'GGC
Cuts at: 758 2284 758
Size: 1526 2577
NarI GG'CG_CC
Cuts at: 1534 1782 1534
Size: 248 3855
NcoI C'CATG_G
Cuts at: 107 107
Size: 4103
NgoMIV G'CCGG_C
Cuts at: 756 2282 756
Size: 1526 2577
NspI r_CATG'y
Cuts at: 469 510 1156 1656 2563 469
Size: 41 646 500 907 2009
Fragments arranged by size:
2009 907 646 500 41
PciI A'CATG_T
Cuts at: 2559 2559
Size: 4103
PfoI T'CCnGG_A
Cuts at: 875 875
Size: 4103
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 4071
PshAI GACnn'nnGTC
Cuts at: 273 273
Size: 4103
PsiI TTA'TAA
Cuts at: 172 172
Size: 4103
PstI C_TGCA'G
Cuts at: 242 1150 1835 242
Size: 908 685 2510
Fragments arranged by size:
2510 908 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 225 237 812 929 1528 1888 225
Size: 12 575 117 599 360 2440
Fragments arranged by size:
2440 599 575 360 117 12
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 1117 1117
Size: 4103
SacII CC_GC'GG
Cuts at: 644 723 644
Size: 79 4024
SalI G'TCGA_C
Cuts at: 1140 1140
Size: 4103
SapI GCTCTTCn'nnn_
Cuts at: 316 2126 2336 316
Size: 1810 210 2083
Fragments arranged by size:
2083 1810 210
SbfI CC_TGCA'GG
Cuts at: 1150 1150
Size: 4103
ScaI AGT'ACT
Cuts at: 3620 3620
Size: 4103
SfcI C'TryA_G
Cuts at: 33 238 1146 1831 2824 3015 33
Size: 205 908 685 993 191 1121
Fragments arranged by size:
1121 993 908 685 205 191
SfoI GGC'GCC
Cuts at: 1535 1783 1535
Size: 248 3855
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 1125 1125
Size: 4103
SmlI C'TyrA_G
Cuts at: 247 1013 1258 2173 2665 2927 247
Size: 766 245 915 492 262 1423
Fragments arranged by size:
1423 915 766 492 262 245
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 469 1156 1656 469
Size: 687 500 2916
Fragments arranged by size:
2916 687 500
StuI AGG'CCT
Cuts at: 703 745 995 703
Size: 42 250 3811
Fragments arranged by size:
3811 250 42
StyI C'CwwG_G
Cuts at: 107 550 1237 1591 107
Size: 443 687 354 2619
Fragments arranged by size:
2619 687 443 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1686 1819 2278 2487 1686
Size: 133 459 209 3302
Fragments arranged by size:
3302 459 209 133
TatI w'GTAC_w
Cuts at: 335 898 3618 335
Size: 563 2720 820
Fragments arranged by size:
2720 820 563
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4071
Tth111I GACn'n_nGTC
Cuts at: 1900 1900
Size: 4103
XbaI T'CTAG_A
Cuts at: 1134 1134
Size: 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 816 1598 816
Size: 782 3321
XmaI C'CCGG_G
Cuts at: 1123 1123
Size: 4103
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI