Restriction map for
pF1KB7375
(Circular) (Six-base) MAPSORT of: pF1KB7375.seq Check: 7686 from: 1 to: 4046
pF1KB7375 4046 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1084 1084
Size: 4046
Acc65I G'GTAC_C
Cuts at: 1062 1062
Size: 4046
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 339 481 1042 1871 2303 3050 3318 339
Size: 142 561 829 432 747 268 1067
Fragments arranged by size:
1067 829 747 561 432 268 142
AflIII A'CryG_T
Cuts at: 1398 2502 3479 1398
Size: 1104 977 1965
Fragments arranged by size:
1965 1104 977
AgeI A'CCGG_T
Cuts at: 2434 2434
Size: 4046
AhdI GACnn_n'nnGTC
Cuts at: 703 703
Size: 4046
AlwNI CAG_nnn'CTG
Cuts at: 1351 2918 1351
Size: 1567 2479
ApaLI G'TGCA_C
Cuts at: 2816 2816
Size: 4046
ApoI r'AATT_y
Cuts at: 626 1050 3399 3655 626
Size: 424 2349 256 1017
Fragments arranged by size:
2349 1017 424 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4046
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 1066 1066
Size: 4046
BamHI G'GATC_C
Cuts at: 1071 1071
Size: 4046
BanI G'GyrC_C
Cuts at: 1062 1476 1724 1759 1062
Size: 414 248 35 3349
Fragments arranged by size:
3349 414 248 35
BanII G_rGCy'C
Cuts at: 796 1060 2090 796
Size: 264 1030 2752
Fragments arranged by size:
2752 1030 264
BbeI G_GCGC'C
Cuts at: 1480 1728 1480
Size: 248 3798
BbsI GAAGACnn'nnnn_
Cuts at: 525 525
Size: 4046
BciVI GTATCCnnnnn_n'
Cuts at: 1944 2705 4046 1944
Size: 761 1341 1944
Fragments arranged by size:
1944 1341 761
BclI T'GATC_A
Cuts at: 1566 1566
Size: 4046
BglII A'GATC_T
Cuts at: 1561 3598 1561
Size: 2037 2009
BlpI GC'TnA_GC
Cuts at: 1158 1158
Size: 4046
Bme1580I G_kGCm'C
Cuts at: 1008 1671 1764 2820 1008
Size: 663 93 1056 2234
Fragments arranged by size:
2234 1056 663 93
BmrI ACTGGGnnnn_n'
Cuts at: 1258 1339 1674 3818 1258
Size: 81 335 2144 1486
Fragments arranged by size:
2144 1486 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 2101 2593 2891 1222
Size: 879 492 298 2377
Fragments arranged by size:
2377 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 569 882 3750 569
Size: 313 2868 865
Fragments arranged by size:
2868 865 313
BsaAI yAC'GTr
Cuts at: 1399 1399
Size: 4046
BsaBI GATnn'nnATC
Cuts at: 6 1560 3597 6
Size: 1554 2037 455
Fragments arranged by size:
2037 1554 455
BsaHI Gr'CG_yC
Cuts at: 1477 1725 2427 3923 1477
Size: 248 702 1496 1600
Fragments arranged by size:
1600 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1235 1756 2434 2708 2855 3266 1235
Size: 521 678 274 147 411 2015
Fragments arranged by size:
2015 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 283 822 238
Size: 45 539 3462
Fragments arranged by size:
3462 539 45
BseYI C'CCAG_C
Cuts at: 150 1471 2229 2806 3302 150
Size: 1321 758 577 496 894
Fragments arranged by size:
1321 894 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 657 315
Size: 342 3704
BsiEI CG_ry'CG
Cuts at: 104 1588 1634 2842 104
Size: 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1060 1838 2028 2820 1060
Size: 778 190 792 2286
Fragments arranged by size:
2286 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 796 1008 1060 1671 1764 1838 2028 2090 2820
Size: 212 52 611 93 74 190 62 730
Cuts at: 2820 796
Size: 2022
Fragments arranged by size:
2022 730 611 212 190 93 74 62
52
BspEI T'CCGG_A
Cuts at: 3266 3266
Size: 4046
BspHI T'CATG_A
Cuts at: 512 536 3222 512
Size: 24 2686 1336
Fragments arranged by size:
2686 1336 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 382 835 1096 1310 1612 1993 382
Size: 453 261 214 302 381 2435
Fragments arranged by size:
2435 453 381 302 261 214
BsrBI CCG'CTC
Cuts at: 50 2338 2392 3876 4041 50
Size: 2288 54 1484 165 55
Fragments arranged by size:
2288 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1958 1958
Size: 4046
BsrFI r'CCGG_y
Cuts at: 2044 2225 2434 2044
Size: 181 209 3656
Fragments arranged by size:
3656 209 181
BsrGI T'GTAC_A
Cuts at: 881 881
Size: 4046
BssSI C'ACGA_G
Cuts at: 2317 2675 2317
Size: 358 3688
BstAPI GCAn_nnn'nTGC
Cuts at: 1423 1423
Size: 4046
BstBI TT'CG_AA
Cuts at: 2407 2407
Size: 4046
BstYI r'GATC_y
Cuts at: 1 1071 1538 1561 1896 2142 3154 3240 3252
Size: 1070 467 23 335 246 1012 86 12
Cuts at: 3252 3598 1
Size: 346 449
Fragments arranged by size:
1070 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 239 107
Size: 132 3914
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 1289 1289
Size: 4046
DraI TTT'AAA
Cuts at: 1045 1045
Size: 4046
DrdI GACnn_nn'nnGTC
Cuts at: 561 1752 2610 561
Size: 1191 858 1997
Fragments arranged by size:
1997 1191 858
EaeI y'GGCC_r
Cuts at: 1631 1805 2196 2223 3568 1631
Size: 174 391 27 1345 2109
Fragments arranged by size:
2109 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1631 1631
Size: 4046
EarI CTCTTCn'nnn_
Cuts at: 500 2069 2279 500
Size: 1569 210 2267
Fragments arranged by size:
2267 1569 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2254 2564 2710 3856 2254
Size: 310 146 1146 2444
Fragments arranged by size:
2444 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1058 1058
Size: 4046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 339 481 1042 1871 2303 3050 3318 339
Size: 142 561 829 432 747 268 1067
Fragments arranged by size:
1067 829 747 561 432 268 142
EcoO109I rG'GnC_Cy
Cuts at: 1185 1185
Size: 4046
EcoRI G'AATT_C
Cuts at: 1050 1050
Size: 4046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 369 2065 369
Size: 1696 2350
FspI TGC'GCA
Cuts at: 1827 1827
Size: 4046
HaeII r_GCGC'y
Cuts at: 1480 1728 2750 3729 1480
Size: 248 1022 979 1797
Fragments arranged by size:
1797 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 689 721 767 799 1888 1918 1920 1950 689
Size: 32 46 32 1089 30 2 30 2785
Fragments arranged by size:
2785 1089 46 32 32 30 30 2
HincII GTy'rAC
Cuts at: 301 1085 301
Size: 784 3262
KasI G'GCGC_C
Cuts at: 1476 1724 1476
Size: 248 3798
KpnI G_GTAC'C
Cuts at: 1066 1066
Size: 4046
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 831 2717 2901 831
Size: 1886 184 1976
Fragments arranged by size:
1976 1886 184
MscI TGG'CCA
Cuts at: 1807 1807
Size: 4046
MslI CAynn'nnrTG
Cuts at: 280 511 787 883 955 2035 2162 280
Size: 231 276 96 72 1080 127 2164
Fragments arranged by size:
2164 1080 276 231 127 96 72
MspA1I CmG'CkG
Cuts at: 592 1157 1471 1831 2844 3089 592
Size: 565 314 360 1013 245 1549
Fragments arranged by size:
1549 1013 565 360 314 245
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 4046
NarI GG'CG_CC
Cuts at: 1477 1725 1477
Size: 248 3798
NcoI C'CATG_G
Cuts at: 107 239 107
Size: 132 3914
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 4046
NspI r_CATG'y
Cuts at: 1099 1599 2506 1099
Size: 500 907 2639
Fragments arranged by size:
2639 907 500
PciI A'CATG_T
Cuts at: 2502 2502
Size: 4046
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4046
PfoI T'CCnGG_A
Cuts at: 550 550
Size: 4046
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 4014
PstI C_TGCA'G
Cuts at: 616 1093 1778 616
Size: 477 685 2884
Fragments arranged by size:
2884 685 477
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4046
PvuII CAG'CTG
Cuts at: 592 1471 1831 592
Size: 879 360 2807
Fragments arranged by size:
2807 879 360
RsrII CG'GwC_CG
Cuts at: 2241 2241
Size: 4046
SacI G_AGCT'C
Cuts at: 1060 1060
Size: 4046
SalI G'TCGA_C
Cuts at: 1083 1083
Size: 4046
SapI GCTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
SbfI CC_TGCA'GG
Cuts at: 1093 1093
Size: 4046
ScaI AGT'ACT
Cuts at: 3563 3563
Size: 4046
SfcI C'TryA_G
Cuts at: 33 612 1009 1089 1774 2767 2958 33
Size: 579 397 80 685 993 191 1121
Fragments arranged by size:
1121 993 685 579 397 191 80
SfoI GGC'GCC
Cuts at: 1478 1726 1478
Size: 248 3798
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SmaI CCC'GGG
Cuts at: 1068 1068
Size: 4046
SmlI C'TyrA_G
Cuts at: 1201 2116 2608 2870 1201
Size: 915 492 262 2377
Fragments arranged by size:
2377 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 1099 1599 1099
Size: 500 3546
SspI AAT'ATT
Cuts at: 234 234
Size: 4046
StuI AGG'CCT
Cuts at: 817 817
Size: 4046
StyI C'CwwG_G
Cuts at: 107 239 557 1180 1534 107
Size: 132 318 623 354 2619
Fragments arranged by size:
2619 623 354 318 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 190 1629 1762 2221 2430 190
Size: 1439 133 459 209 1806
Fragments arranged by size:
1806 1439 459 209 133
TatI w'GTAC_w
Cuts at: 284 500 881 3561 284
Size: 216 381 2680 769
Fragments arranged by size:
2680 769 381 216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4014
Tth111I GACn'n_nGTC
Cuts at: 1843 1843
Size: 4046
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 793 1541 793
Size: 748 3298
XmaI C'CCGG_G
Cuts at: 1066 1066
Size: 4046
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI