Restriction map for
pF1KB7372
(Circular) (Six-base) MAPSORT of: pF1KB7372.seq Check: 4441 from: 1 to: 3998
pF1KB7372 3998 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AatII G_ACGT'C
Cuts at: 722 722
Size: 3998
AccI GT'mk_AC
Cuts at: 848 1036 848
Size: 188 3810
Acc65I G'GTAC_C
Cuts at: 1014 1014
Size: 3998
AclI AA'CG_TT
Cuts at: 3838 3838
Size: 3998
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1823 2255 3002 3270 1823
Size: 432 747 268 2551
Fragments arranged by size:
2551 747 432 268
AflIII A'CryG_T
Cuts at: 746 823 1350 2454 3431 746
Size: 77 527 1104 977 1313
Fragments arranged by size:
1313 1104 977 527 77
AgeI A'CCGG_T
Cuts at: 2386 2386
Size: 3998
AlwNI CAG_nnn'CTG
Cuts at: 1303 2870 1303
Size: 1567 2431
ApaLI G'TGCA_C
Cuts at: 2768 2768
Size: 3998
ApoI r'AATT_y
Cuts at: 1002 3351 3607 1002
Size: 2349 256 1393
Fragments arranged by size:
2349 1393 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3998
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
AvaI C'yCGr_G
Cuts at: 1018 1018
Size: 3998
BamHI G'GATC_C
Cuts at: 1023 1023
Size: 3998
BanI G'GyrC_C
Cuts at: 236 1014 1428 1676 1711 236
Size: 778 414 248 35 2523
Fragments arranged by size:
2523 778 414 248 35
BanII G_rGCy'C
Cuts at: 1012 2042 1012
Size: 1030 2968
BbeI G_GCGC'C
Cuts at: 1432 1680 1432
Size: 248 3750
BbsI GAAGACnn'nnnn_
Cuts at: 765 765
Size: 3998
BciVI GTATCCnnnnn_n'
Cuts at: 1896 2657 3998 1896
Size: 761 1341 1896
Fragments arranged by size:
1896 1341 761
BclI T'GATC_A
Cuts at: 1518 1518
Size: 3998
BfrBI ATG'CAT
Cuts at: 132 162 132
Size: 30 3968
BglII A'GATC_T
Cuts at: 707 1513 3550 707
Size: 806 2037 1155
Fragments arranged by size:
2037 1155 806
BlpI GC'TnA_GC
Cuts at: 1110 1110
Size: 3998
Bme1580I G_kGCm'C
Cuts at: 1623 1716 2772 1623
Size: 93 1056 2849
Fragments arranged by size:
2849 1056 93
BmgBI CAC'GTC
Cuts at: 891 891
Size: 3998
BmrI ACTGGGnnnn_n'
Cuts at: 1210 1291 1626 3770 1210
Size: 81 335 2144 1438
Fragments arranged by size:
2144 1438 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 3998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1174 2053 2545 2843 838
Size: 336 879 492 298 1993
Fragments arranged by size:
1993 879 492 336 298
BsaI GGTCTCn'nnnn_
Cuts at: 3702 3702
Size: 3998
BsaAI yAC'GTr
Cuts at: 749 1351 749
Size: 602 3396
BsaBI GATnn'nnATC
Cuts at: 6 334 1512 3549 6
Size: 328 1178 2037 455
Fragments arranged by size:
2037 1178 455 328
BsaHI Gr'CG_yC
Cuts at: 719 1429 1677 2379 3875 719
Size: 710 248 702 1496 842
Fragments arranged by size:
1496 842 710 702 248
BsaWI w'CCGG_w
Cuts at: 1187 1708 2386 2660 2807 3218 1187
Size: 521 678 274 147 411 1967
Fragments arranged by size:
1967 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 229 259 229
Size: 30 3968
BseYI C'CCAG_C
Cuts at: 206 1423 2181 2758 3254 206
Size: 1217 758 577 496 950
Fragments arranged by size:
1217 950 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 292 632 241
Size: 51 340 3607
Fragments arranged by size:
3607 340 51
BsiEI CG_ry'CG
Cuts at: 104 1540 1586 2794 104
Size: 1436 46 1208 1308
Fragments arranged by size:
1436 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 122 1012 1790 1980 2772 122
Size: 890 778 190 792 1348
Fragments arranged by size:
1348 890 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 122 1012 1623 1716 1790 1980 2042 2772 122
Size: 890 611 93 74 190 62 730 1348
Fragments arranged by size:
1348 890 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3218 3218
Size: 3998
BspHI T'CATG_A
Cuts at: 3174 3174
Size: 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1048 1262 1564 1945 1048
Size: 214 302 381 3101
Fragments arranged by size:
3101 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2290 2344 3828 3993 50
Size: 2240 54 1484 165 55
Fragments arranged by size:
2240 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1910 1910
Size: 3998
BsrFI r'CCGG_y
Cuts at: 1996 2177 2386 1996
Size: 181 209 3608
Fragments arranged by size:
3608 209 181
BssSI C'ACGA_G
Cuts at: 778 2269 2627 778
Size: 1491 358 2149
Fragments arranged by size:
2149 1491 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1375 1375
Size: 3998
BstBI TT'CG_AA
Cuts at: 2359 2359
Size: 3998
BstEII G'GTnAC_C
Cuts at: 258 258
Size: 3998
BstYI r'GATC_y
Cuts at: 1 335 707 1023 1490 1513 1848 2094 3106
Size: 334 372 316 467 23 335 246 1012
Cuts at: 3106 3192 3204 3550 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 372 346 335 334 316
246 86 23 12
BtgI C'CryG_G
Cuts at: 144 536 792 144
Size: 392 256 3350
Fragments arranged by size:
3350 392 256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 171 1948 2129 2292 171
Size: 1777 181 163 1877
Fragments arranged by size:
1877 1777 181 163
BtsI GCAGTG_nn'
Cuts at: 229 1241 229
Size: 1012 2986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 126 161 179 214 444 479 126
Size: 35 18 35 230 35 3645
Fragments arranged by size:
3645 230 35 35 35 18
DraI TTT'AAA
Cuts at: 997 997
Size: 3998
DrdI GACnn_nn'nnGTC
Cuts at: 1704 2562 1704
Size: 858 3140
EaeI y'GGCC_r
Cuts at: 147 1583 1757 2148 2175 3520 147
Size: 1436 174 391 27 1345 625
Fragments arranged by size:
1436 1345 625 391 174 27
EagI C'GGCC_G
Cuts at: 1583 1583
Size: 3998
EarI CTCTTCn'nnn_
Cuts at: 565 722 2021 2231 565
Size: 157 1299 210 2332
Fragments arranged by size:
2332 1299 210 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2206 2516 2662 3808 2206
Size: 310 146 1146 2396
Fragments arranged by size:
2396 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1010 1010
Size: 3998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1823 2255 3002 3270 1823
Size: 432 747 268 2551
Fragments arranged by size:
2551 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 879 879
Size: 3998
EcoO109I rG'GnC_Cy
Cuts at: 1137 1137
Size: 3998
EcoRI G'AATT_C
Cuts at: 1002 1002
Size: 3998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3998
FspI TGC'GCA
Cuts at: 1779 1779
Size: 3998
HaeII r_GCGC'y
Cuts at: 1432 1680 2702 3681 1432
Size: 248 1022 979 1749
Fragments arranged by size:
1749 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1840 1870 1872 1902 1840
Size: 30 2 30 3936
Fragments arranged by size:
3936 30 30 2
HincII GTy'rAC
Cuts at: 1037 1037
Size: 3998
KasI G'GCGC_C
Cuts at: 1428 1676 1428
Size: 248 3750
KpnI G_GTAC'C
Cuts at: 1018 1018
Size: 3998
MluI A'CGCG_T
Cuts at: 3431 3431
Size: 3998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2669 2853 2669
Size: 184 3814
MscI TGG'CCA
Cuts at: 149 1759 149
Size: 1610 2388
MslI CAynn'nnrTG
Cuts at: 1987 2114 1987
Size: 127 3871
MspA1I CmG'CkG
Cuts at: 210 1109 1423 1783 2796 3041 210
Size: 899 314 360 1013 245 1167
Fragments arranged by size:
1167 1013 899 360 314 245
NaeI GCC'GGC
Cuts at: 2179 2179
Size: 3998
NarI GG'CG_CC
Cuts at: 1429 1677 1429
Size: 248 3750
NcoI C'CATG_G
Cuts at: 536 536
Size: 3998
NgoMIV G'CCGG_C
Cuts at: 2177 2177
Size: 3998
NsiI A_TGCA'T
Cuts at: 134 164 134
Size: 30 3968
NspI r_CATG'y
Cuts at: 132 136 166 660 1051 1551 2458 132
Size: 4 30 494 391 500 907 1672
Fragments arranged by size:
1672 907 500 494 391 30 4
PasI CC'CwG_GG
Cuts at: 875 875
Size: 3998
PciI A'CATG_T
Cuts at: 2454 2454
Size: 3998
PmeI GTTT'AAAC
Cuts at: 997 997
Size: 3998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3169 3137
Size: 32 3966
PsiI TTA'TAA
Cuts at: 419 419
Size: 3998
PstI C_TGCA'G
Cuts at: 1045 1730 1045
Size: 685 3313
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3998
PvuII CAG'CTG
Cuts at: 210 1423 1783 210
Size: 1213 360 2425
Fragments arranged by size:
2425 1213 360
RsrII CG'GwC_CG
Cuts at: 2193 2193
Size: 3998
SacI G_AGCT'C
Cuts at: 1012 1012
Size: 3998
SalI G'TCGA_C
Cuts at: 1035 1035
Size: 3998
SapI GCTCTTCn'nnn_
Cuts at: 2021 2231 2021
Size: 210 3788
SbfI CC_TGCA'GG
Cuts at: 1045 1045
Size: 3998
ScaI AGT'ACT
Cuts at: 3515 3515
Size: 3998
SfcI C'TryA_G
Cuts at: 33 642 1041 1726 2719 2910 33
Size: 609 399 685 993 191 1121
Fragments arranged by size:
1121 993 685 609 399 191
SfoI GGC'GCC
Cuts at: 1430 1678 1430
Size: 248 3750
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
SmaI CCC'GGG
Cuts at: 1020 1020
Size: 3998
SmlI C'TyrA_G
Cuts at: 853 1153 2068 2560 2822 853
Size: 300 915 492 262 2029
Fragments arranged by size:
2029 915 492 300 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3998
SphI G_CATG'C
Cuts at: 132 136 166 660 1051 1551 132
Size: 4 30 494 391 500 2579
Fragments arranged by size:
2579 500 494 391 30 4
StuI AGG'CCT
Cuts at: 885 885
Size: 3998
StyI C'CwwG_G
Cuts at: 194 406 536 963 1132 1486 194
Size: 212 130 427 169 354 2706
Fragments arranged by size:
2706 427 354 212 169 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 254 1581 1714 2173 2382 254
Size: 1327 133 459 209 1870
Fragments arranged by size:
1870 1327 459 209 133
TatI w'GTAC_w
Cuts at: 393 3513 393
Size: 3120 878
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 229 261 3515 3547 229
Size: 32 3254 32 680
Fragments arranged by size:
3254 680 32 32
Tth111I GACn'n_nGTC
Cuts at: 1795 1795
Size: 3998
XbaI T'CTAG_A
Cuts at: 1029 1029
Size: 3998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1493 1493
Size: 3998
XmaI C'CCGG_G
Cuts at: 1018 1018
Size: 3998
ZraI GAC'GTC
Cuts at: 720 720
Size: 3998
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmtI BpmI
Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI