Restriction map for
pF1KB7371
(Circular) (Six-base) MAPSORT of: pF1KB7371.seq Check: 3367 from: 1 to: 4061
pF1KB7371 4061 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 844 844
Size: 4061
AccI GT'mk_AC
Cuts at: 1099 1099
Size: 4061
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 343 420 1886 2318 3065 3333 212
Size: 131 77 1466 432 747 268 940
Fragments arranged by size:
1466 940 747 432 268 131 77
AflII C'TTAA_G
Cuts at: 193 193
Size: 4061
AflIII A'CryG_T
Cuts at: 1413 2517 3494 1413
Size: 1104 977 1980
Fragments arranged by size:
1980 1104 977
AgeI A'CCGG_T
Cuts at: 2449 2449
Size: 4061
AlwNI CAG_nnn'CTG
Cuts at: 1035 1366 2933 1035
Size: 331 1567 2163
Fragments arranged by size:
2163 1567 331
ApaI G_GGCC'C
Cuts at: 718 718
Size: 4061
ApaLI G'TGCA_C
Cuts at: 2831 2831
Size: 4061
ApoI r'AATT_y
Cuts at: 1065 3414 3670 1065
Size: 2349 256 1456
Fragments arranged by size:
2349 1456 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 133 1081 133
Size: 948 3113
BamHI G'GATC_C
Cuts at: 349 1086 349
Size: 737 3324
BanI G'GyrC_C
Cuts at: 924 1077 1491 1739 1774 924
Size: 153 414 248 35 3211
Fragments arranged by size:
3211 414 248 153 35
BanII G_rGCy'C
Cuts at: 491 718 1075 2105 491
Size: 227 357 1030 2447
Fragments arranged by size:
2447 1030 357 227
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BbsI GAAGACnn'nnnn_
Cuts at: 218 372 218
Size: 154 3907
BciVI GTATCCnnnnn_n'
Cuts at: 1959 2720 4061 1959
Size: 761 1341 1959
Fragments arranged by size:
1959 1341 761
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BglII A'GATC_T
Cuts at: 587 1576 3613 587
Size: 989 2037 1035
Fragments arranged by size:
2037 1035 989
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 682 718 1686 1779 2835 682
Size: 36 968 93 1056 1908
Fragments arranged by size:
1908 1056 968 93 36
BmgBI CAC'GTC
Cuts at: 267 267
Size: 4061
BmrI ACTGGGnnnn_n'
Cuts at: 1273 1354 1689 3833 1273
Size: 81 335 2144 1501
Fragments arranged by size:
2144 1501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 571 576 475
Size: 96 5 3960
Fragments arranged by size:
3960 96 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 2116 2608 2906 1237
Size: 879 492 298 2392
Fragments arranged by size:
2392 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3765 3765
Size: 4061
BsaAI yAC'GTr
Cuts at: 1414 1414
Size: 4061
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 1492 1740 2442 3938 1492
Size: 248 702 1496 1615
Fragments arranged by size:
1615 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1250 1771 2449 2723 2870 3281 1250
Size: 521 678 274 147 411 2030
Fragments arranged by size:
2030 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 612 612
Size: 4061
BseYI C'CCAG_C
Cuts at: 729 1486 2244 2821 3317 729
Size: 757 758 577 496 1473
Fragments arranged by size:
1473 758 757 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 825 555
Size: 270 3791
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2857 104
Size: 1499 46 1208 1308
Fragments arranged by size:
1499 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 134 1075 1853 2043 2835 134
Size: 941 778 190 792 1360
Fragments arranged by size:
1360 941 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 134 491 682 718 1075 1686 1779 1853 2043
Size: 357 191 36 357 611 93 74 190
Cuts at: 2043 2105 2835 134
Size: 62 730 1360
Fragments arranged by size:
1360 730 611 357 357 191 190 93
74 62 36
BspEI T'CCGG_A
Cuts at: 3281 3281
Size: 4061
BspHI T'CATG_A
Cuts at: 3237 3237
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 1111 1325 1627 2008 844
Size: 267 214 302 381 2897
Fragments arranged by size:
2897 381 302 267 214
BsrBI CCG'CTC
Cuts at: 50 2353 2407 3891 4056 50
Size: 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 943 1045 1973 943
Size: 102 928 3031
Fragments arranged by size:
3031 928 102
BsrFI r'CCGG_y
Cuts at: 2059 2240 2449 2059
Size: 181 209 3671
Fragments arranged by size:
3671 209 181
BssSI C'ACGA_G
Cuts at: 409 2332 2690 409
Size: 1923 358 1780
Fragments arranged by size:
1923 1780 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1035 1438 1035
Size: 403 3658
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstYI r'GATC_y
Cuts at: 1 349 534 587 641 972 1086 1553 1576
Size: 348 185 53 54 331 114 467 23
Cuts at: 1576 1911 2157 3169 3255 3267 3613 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 348 346 335 331 246
185 114 86 54 53 23 12
BtgI C'CryG_G
Cuts at: 107 361 384 107
Size: 254 23 3784
Fragments arranged by size:
3784 254 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 1304 1304
Size: 4061
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 523 1767 2625 523
Size: 1244 858 1959
Fragments arranged by size:
1959 1244 858
EaeI y'GGCC_r
Cuts at: 1646 1820 2211 2238 3583 1646
Size: 174 391 27 1345 2124
Fragments arranged by size:
2124 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 280 308 802 982 2084 2294 280
Size: 28 494 180 1102 210 2047
Fragments arranged by size:
2047 1102 494 210 180 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 2579 2725 3871 2269
Size: 310 146 1146 2459
Fragments arranged by size:
2459 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1073 1073
Size: 4061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 343 420 475 571 576 1886 2318 3065
Size: 131 77 55 96 5 1310 432 747
Cuts at: 3065 3333 212
Size: 268 940
Fragments arranged by size:
1310 940 747 432 268 131 96 77
55 5
EcoO109I rG'GnC_Cy
Cuts at: 318 516 714 715 857 954 1200 318
Size: 198 198 1 142 97 246 3179
Fragments arranged by size:
3179 246 198 198 142 97 1
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 4061
FspI TGC'GCA
Cuts at: 1842 1842
Size: 4061
HaeII r_GCGC'y
Cuts at: 1495 1743 2765 3744 1495
Size: 248 1022 979 1812
Fragments arranged by size:
1812 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 109 141 334 366 604 636 1903 1933 1935
Size: 32 193 32 238 32 1267 30 2
Cuts at: 1935 1965 109
Size: 30 2205
Fragments arranged by size:
2205 1267 238 193 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1100 1100
Size: 4061
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 649 2732 2916 649
Size: 2083 184 1794
Fragments arranged by size:
2083 1794 184
MscI TGG'CCA
Cuts at: 1822 1822
Size: 4061
MslI CAynn'nnrTG
Cuts at: 655 2050 2177 655
Size: 1395 127 2539
Fragments arranged by size:
2539 1395 127
MspA1I CmG'CkG
Cuts at: 1172 1486 1846 2859 3104 1172
Size: 314 360 1013 245 2129
Fragments arranged by size:
2129 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NcoI C'CATG_G
Cuts at: 107 361 107
Size: 254 3807
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NspI r_CATG'y
Cuts at: 1114 1614 2521 1114
Size: 500 907 2654
Fragments arranged by size:
2654 907 500
PciI A'CATG_T
Cuts at: 2517 2517
Size: 4061
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 521 553 3200 3232 521
Size: 32 2647 32 1350
Fragments arranged by size:
2647 1350 32 32
PpuMI rG'GwC_Cy
Cuts at: 516 857 954 516
Size: 341 97 3623
Fragments arranged by size:
3623 341 97
PspOMI G'GGCC_C
Cuts at: 714 714
Size: 4061
PstI C_TGCA'G
Cuts at: 1108 1793 1108
Size: 685 3376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 1486 1846 1486
Size: 360 3701
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 1075 1075
Size: 4061
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 1104 1789 2782 2973 33
Size: 1071 685 993 191 1121
Fragments arranged by size:
1121 1071 993 685 191
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 193 1216 2131 2623 2885 193
Size: 1023 915 492 262 1369
Fragments arranged by size:
1369 1023 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StuI AGG'CCT
Cuts at: 259 259
Size: 4061
StyI C'CwwG_G
Cuts at: 107 361 1195 1549 107
Size: 254 834 354 2619
Fragments arranged by size:
2619 834 354 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1644 1777 2236 2445 1644
Size: 133 459 209 3260
Fragments arranged by size:
3260 459 209 133
TatI w'GTAC_w
Cuts at: 3576 3576
Size: 4061
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 4029
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4061
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 415 1556 415
Size: 1141 2920
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI