Restriction map for
pF1KB7370
(Circular) (Six-base) MAPSORT of: pF1KB7370.seq Check: 164 from: 1 to: 3704
pF1KB7370 3704 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 742 742
Size: 3704
Acc65I G'GTAC_C
Cuts at: 720 720
Size: 3704
AclI AA'CG_TT
Cuts at: 3544 3544
Size: 3704
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 370 527 1529 1961 2708 2976 145
Size: 225 157 1002 432 747 268 873
Fragments arranged by size:
1002 873 747 432 268 225 157
AflIII A'CryG_T
Cuts at: 1056 2160 3137 1056
Size: 1104 977 1623
Fragments arranged by size:
1623 1104 977
AgeI A'CCGG_T
Cuts at: 2092 2092
Size: 3704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 327 327
Size: 3704
AlwNI CAG_nnn'CTG
Cuts at: 1009 2576 1009
Size: 1567 2137
ApaLI G'TGCA_C
Cuts at: 2474 2474
Size: 3704
ApoI r'AATT_y
Cuts at: 317 708 3057 3313 317
Size: 391 2349 256 708
Fragments arranged by size:
2349 708 391 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3704
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
AvaI C'yCGr_G
Cuts at: 269 724 269
Size: 455 3249
BamHI G'GATC_C
Cuts at: 729 729
Size: 3704
BanI G'GyrC_C
Cuts at: 497 720 1134 1382 1417 497
Size: 223 414 248 35 2784
Fragments arranged by size:
2784 414 248 223 35
BanII G_rGCy'C
Cuts at: 718 1748 718
Size: 1030 2674
BbeI G_GCGC'C
Cuts at: 1138 1386 1138
Size: 248 3456
BbsI GAAGACnn'nnnn_
Cuts at: 350 467 350
Size: 117 3587
BciVI GTATCCnnnnn_n'
Cuts at: 1602 2363 3704 1602
Size: 761 1341 1602
Fragments arranged by size:
1602 1341 761
BclI T'GATC_A
Cuts at: 1224 1224
Size: 3704
BglI GCCn_nnn'nGGC
Cuts at: 434 434
Size: 3704
BglII A'GATC_T
Cuts at: 1219 3256 1219
Size: 2037 1667
BlpI GC'TnA_GC
Cuts at: 816 816
Size: 3704
Bme1580I G_kGCm'C
Cuts at: 306 1329 1422 2478 306
Size: 1023 93 1056 1532
Fragments arranged by size:
1532 1056 1023 93
BmrI ACTGGGnnnn_n'
Cuts at: 916 997 1332 3476 916
Size: 81 335 2144 1144
Fragments arranged by size:
2144 1144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 3704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 621 621
Size: 3704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 880 1759 2251 2549 408
Size: 472 879 492 298 1563
Fragments arranged by size:
1563 879 492 472 298
BsaI GGTCTCn'nnnn_
Cuts at: 3408 3408
Size: 3704
BsaAI yAC'GTr
Cuts at: 1057 1057
Size: 3704
BsaBI GATnn'nnATC
Cuts at: 6 1218 3255 6
Size: 1212 2037 455
Fragments arranged by size:
2037 1212 455
BsaHI Gr'CG_yC
Cuts at: 1135 1383 2085 3581 1135
Size: 248 702 1496 1258
Fragments arranged by size:
1496 1258 702 248
BsaWI w'CCGG_w
Cuts at: 893 1414 2092 2366 2513 2924 893
Size: 521 678 274 147 411 1673
Fragments arranged by size:
1673 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 602 632 602
Size: 30 3674
BseYI C'CCAG_C
Cuts at: 366 405 1129 1887 2464 2960 366
Size: 39 724 758 577 496 1110
Fragments arranged by size:
1110 758 724 577 496 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 415
Size: 3704
BsiEI CG_ry'CG
Cuts at: 104 1246 1292 2500 104
Size: 1142 46 1208 1308
Fragments arranged by size:
1308 1208 1142 46
BsiHKAI G_wGCw'C
Cuts at: 718 1496 1686 2478 718
Size: 778 190 792 1944
Fragments arranged by size:
1944 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 355 355
Size: 3704
Bsp1286I G_dGCh'C
Cuts at: 306 718 1329 1422 1496 1686 1748 2478 306
Size: 412 611 93 74 190 62 730 1532
Fragments arranged by size:
1532 730 611 412 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2924 2924
Size: 3704
BspHI T'CATG_A
Cuts at: 2880 2880
Size: 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 754 968 1270 1651 754
Size: 214 302 381 2807
Fragments arranged by size:
2807 381 302 214
BsrBI CCG'CTC
Cuts at: 50 594 1996 2050 3534 3699 50
Size: 544 1402 54 1484 165 55
Fragments arranged by size:
1484 1402 544 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1616 1616
Size: 3704
BsrFI r'CCGG_y
Cuts at: 1702 1883 2092 1702
Size: 181 209 3314
Fragments arranged by size:
3314 209 181
BssSI C'ACGA_G
Cuts at: 1975 2333 1975
Size: 358 3346
BstAPI GCAn_nnn'nTGC
Cuts at: 1081 1081
Size: 3704
BstBI TT'CG_AA
Cuts at: 2065 2065
Size: 3704
BstYI r'GATC_y
Cuts at: 1 634 729 1196 1219 1554 1800 2812 2898
Size: 633 95 467 23 335 246 1012 86
Cuts at: 2898 2910 3256 1
Size: 12 346 449
Fragments arranged by size:
1012 633 467 449 346 335 246 95
86 23 12
BtgI C'CryG_G
Cuts at: 107 155 107
Size: 48 3656
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1654 1835 1998 1654
Size: 181 163 3360
Fragments arranged by size:
3360 181 163
BtsI GCAGTG_nn'
Cuts at: 398 568 947 398
Size: 170 379 3155
Fragments arranged by size:
3155 379 170
DraI TTT'AAA
Cuts at: 703 703
Size: 3704
DrdI GACnn_nn'nnGTC
Cuts at: 1410 2268 1410
Size: 858 2846
EaeI y'GGCC_r
Cuts at: 110 1289 1463 1854 1881 3226 110
Size: 1179 174 391 27 1345 588
Fragments arranged by size:
1345 1179 588 391 174 27
EagI C'GGCC_G
Cuts at: 1289 1289
Size: 3704
EarI CTCTTCn'nnn_
Cuts at: 127 215 389 1727 1937 127
Size: 88 174 1338 210 1894
Fragments arranged by size:
1894 1338 210 174 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1912 2222 2368 3514 1912
Size: 310 146 1146 2102
Fragments arranged by size:
2102 1146 310 146
EcoICRI GAG'CTC
Cuts at: 716 716
Size: 3704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 370 527 621 1529 1961 2708 2976 145
Size: 225 157 94 908 432 747 268 873
Fragments arranged by size:
908 873 747 432 268 225 157 94
EcoO109I rG'GnC_Cy
Cuts at: 120 843 120
Size: 723 2981
EcoRI G'AATT_C
Cuts at: 708 708
Size: 3704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 468 557 1723 468
Size: 89 1166 2449
Fragments arranged by size:
2449 1166 89
FspI TGC'GCA
Cuts at: 1485 1485
Size: 3704
HaeII r_GCGC'y
Cuts at: 1138 1386 2408 3387 1138
Size: 248 1022 979 1455
Fragments arranged by size:
1455 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1546 1576 1578 1608 1546
Size: 30 2 30 3642
Fragments arranged by size:
3642 30 30 2
HincII GTy'rAC
Cuts at: 743 743
Size: 3704
HindIII A'AGCT_T
Cuts at: 445 445
Size: 3704
KasI G'GCGC_C
Cuts at: 1134 1382 1134
Size: 248 3456
KpnI G_GTAC'C
Cuts at: 724 724
Size: 3704
MluI A'CGCG_T
Cuts at: 3137 3137
Size: 3704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2375 2559 2375
Size: 184 3520
MscI TGG'CCA
Cuts at: 1465 1465
Size: 3704
MslI CAynn'nnrTG
Cuts at: 309 1693 1820 309
Size: 1384 127 2193
Fragments arranged by size:
2193 1384 127
MspA1I CmG'CkG
Cuts at: 815 1129 1489 2502 2747 815
Size: 314 360 1013 245 1772
Fragments arranged by size:
1772 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1885 1885
Size: 3704
NarI GG'CG_CC
Cuts at: 1135 1383 1135
Size: 248 3456
NcoI C'CATG_G
Cuts at: 107 155 107
Size: 48 3656
NgoMIV G'CCGG_C
Cuts at: 1883 1883
Size: 3704
NspI r_CATG'y
Cuts at: 314 422 757 1257 2164 314
Size: 108 335 500 907 1854
Fragments arranged by size:
1854 907 500 335 108
PciI A'CATG_T
Cuts at: 2160 2160
Size: 3704
PflMI CCAn_nnn'nTGG
Cuts at: 156 156
Size: 3704
PmeI GTTT'AAAC
Cuts at: 703 703
Size: 3704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2843 2875 2843
Size: 32 3672
PpuMI rG'GwC_Cy
Cuts at: 120 120
Size: 3704
PstI C_TGCA'G
Cuts at: 751 1436 751
Size: 685 3019
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3704
PvuII CAG'CTG
Cuts at: 1129 1489 1129
Size: 360 3344
RsrII CG'GwC_CG
Cuts at: 1899 1899
Size: 3704
SacI G_AGCT'C
Cuts at: 718 718
Size: 3704
SalI G'TCGA_C
Cuts at: 741 741
Size: 3704
SapI GCTCTTCn'nnn_
Cuts at: 389 1727 1937 389
Size: 1338 210 2156
Fragments arranged by size:
2156 1338 210
SbfI CC_TGCA'GG
Cuts at: 751 751
Size: 3704
ScaI AGT'ACT
Cuts at: 3221 3221
Size: 3704
SfcI C'TryA_G
Cuts at: 33 251 747 1432 2425 2616 33
Size: 218 496 685 993 191 1121
Fragments arranged by size:
1121 993 685 496 218 191
SfoI GGC'GCC
Cuts at: 1136 1384 1136
Size: 248 3456
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
SmaI CCC'GGG
Cuts at: 726 726
Size: 3704
SmlI C'TyrA_G
Cuts at: 423 859 1774 2266 2528 423
Size: 436 915 492 262 1599
Fragments arranged by size:
1599 915 492 436 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3704
SphI G_CATG'C
Cuts at: 314 757 1257 314
Size: 443 500 2761
Fragments arranged by size:
2761 500 443
StyI C'CwwG_G
Cuts at: 107 155 838 1192 107
Size: 48 683 354 2619
Fragments arranged by size:
2619 683 354 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 148 1287 1420 1879 2088 148
Size: 1139 133 459 209 1764
Fragments arranged by size:
1764 1139 459 209 133
TatI w'GTAC_w
Cuts at: 3219 3219
Size: 3704
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 3672
Tth111I GACn'n_nGTC
Cuts at: 118 1501 118
Size: 1383 2321
XbaI T'CTAG_A
Cuts at: 456 735 456
Size: 279 3425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1199 1199
Size: 3704
XmaI C'CCGG_G
Cuts at: 724 724
Size: 3704
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BseRI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI