Restriction map for
pF1KB7368
(Circular) (Six-base) MAPSORT of: pF1KB7368.seq Check: 1769 from: 1 to: 4061
pF1KB7368 4061 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 346 346
Size: 4061
AccI GT'mk_AC
Cuts at: 1099 1099
Size: 4061
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 1886 2318 3065 3333 704
Size: 1182 432 747 268 1432
Fragments arranged by size:
1432 1182 747 432 268
AflIII A'CryG_T
Cuts at: 1413 2517 3494 1413
Size: 1104 977 1980
Fragments arranged by size:
1980 1104 977
AgeI A'CCGG_T
Cuts at: 2449 2449
Size: 4061
AleI CACnn'nnGTG
Cuts at: 851 851
Size: 4061
AlwNI CAG_nnn'CTG
Cuts at: 1366 2933 1366
Size: 1567 2494
ApaLI G'TGCA_C
Cuts at: 2831 2831
Size: 4061
ApoI r'AATT_y
Cuts at: 237 776 1010 1065 3414 3670 237
Size: 539 234 55 2349 256 628
Fragments arranged by size:
2349 628 539 256 234 55
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 1081 1081
Size: 4061
AvrII C'CTAG_G
Cuts at: 735 735
Size: 4061
BamHI G'GATC_C
Cuts at: 1086 1086
Size: 4061
BanI G'GyrC_C
Cuts at: 1077 1491 1739 1774 1077
Size: 414 248 35 3364
Fragments arranged by size:
3364 414 248 35
BanII G_rGCy'C
Cuts at: 599 1075 2105 599
Size: 476 1030 2555
Fragments arranged by size:
2555 1030 476
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BbsI GAAGACnn'nnnn_
Cuts at: 123 849 1006 123
Size: 726 157 3178
Fragments arranged by size:
3178 726 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 428 462 90
Size: 34 304 34 3689
Fragments arranged by size:
3689 304 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 463 505 1959 2720 4061 463
Size: 42 1454 761 1341 463
Fragments arranged by size:
1454 1341 761 463 42
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BfrBI ATG'CAT
Cuts at: 839 839
Size: 4061
BglI GCCn_nnn'nGGC
Cuts at: 310 310
Size: 4061
BglII A'GATC_T
Cuts at: 1576 3613 1576
Size: 2037 2024
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 1686 1779 2835 1686
Size: 93 1056 2912
Fragments arranged by size:
2912 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1273 1354 1689 3833 1273
Size: 81 335 2144 1501
Fragments arranged by size:
2144 1501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 193
Size: 4061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 1237 2116 2608 2906 522
Size: 715 879 492 298 1677
Fragments arranged by size:
1677 879 715 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3765 3765
Size: 4061
BsaAI yAC'GTr
Cuts at: 1414 1414
Size: 4061
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 1492 1740 2442 3938 1492
Size: 248 702 1496 1615
Fragments arranged by size:
1615 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1250 1771 2449 2723 2870 3281 1250
Size: 521 678 274 147 411 2030
Fragments arranged by size:
2030 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 524 1486 2244 2821 3317 524
Size: 962 758 577 496 1268
Fragments arranged by size:
1268 962 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 202
Size: 4061
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2857 104
Size: 1499 46 1208 1308
Fragments arranged by size:
1499 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 659 1075 1853 2043 2835 659
Size: 416 778 190 792 1885
Fragments arranged by size:
1885 792 778 416 190
BsmI GAATG_Cn'
Cuts at: 341 341
Size: 4061
Bsp1286I G_dGCh'C
Cuts at: 599 659 1075 1686 1779 1853 2043 2105 2835
Size: 60 416 611 93 74 190 62 730
Cuts at: 2835 599
Size: 1825
Fragments arranged by size:
1825 730 611 416 190 93 74 62
60
BspEI T'CCGG_A
Cuts at: 3281 3281
Size: 4061
BspHI T'CATG_A
Cuts at: 3237 3237
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 346 1111 1325 1627 2008 346
Size: 765 214 302 381 2399
Fragments arranged by size:
2399 765 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2353 2407 3891 4056 50
Size: 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 323 470 839 1973 323
Size: 147 369 1134 2411
Fragments arranged by size:
2411 1134 369 147
BsrFI r'CCGG_y
Cuts at: 2059 2240 2449 2059
Size: 181 209 3671
Fragments arranged by size:
3671 209 181
BsrGI T'GTAC_A
Cuts at: 290 290
Size: 4061
BssSI C'ACGA_G
Cuts at: 2332 2690 2332
Size: 358 3703
BstAPI GCAn_nnn'nTGC
Cuts at: 1438 1438
Size: 4061
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstYI r'GATC_y
Cuts at: 1 1086 1553 1576 1911 2157 3169 3255 3267
Size: 1085 467 23 335 246 1012 86 12
Cuts at: 3267 3613 1
Size: 346 449
Fragments arranged by size:
1085 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 591 591
Size: 4061
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 190 1304 190
Size: 1114 2947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 306 341 306
Size: 35 4026
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 1767 2625 1767
Size: 858 3203
EaeI y'GGCC_r
Cuts at: 302 485 662 1646 1820 2211 2238 3583 302
Size: 183 177 984 174 391 27 1345 780
Fragments arranged by size:
1345 984 780 391 183 177 174 27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 2579 2725 3871 2269
Size: 310 146 1146 2459
Fragments arranged by size:
2459 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1073 1073
Size: 4061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 704 1886 2318 3065 3333 193
Size: 511 1182 432 747 268 921
Fragments arranged by size:
1182 921 747 511 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 590 590
Size: 4061
EcoO109I rG'GnC_Cy
Cuts at: 1200 1200
Size: 4061
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
EcoRV GAT'ATC
Cuts at: 690 690
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 4061
FspI TGC'GCA
Cuts at: 1842 1842
Size: 4061
HaeII r_GCGC'y
Cuts at: 1495 1743 2765 3744 1495
Size: 248 1022 979 1812
Fragments arranged by size:
1812 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 153 185 1903 1933 1935 1965 153
Size: 32 1718 30 2 30 2249
Fragments arranged by size:
2249 1718 32 30 30 2
HincII GTy'rAC
Cuts at: 699 1100 699
Size: 401 3660
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MfeI C'AATT_G
Cuts at: 440 440
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 169 2732 2916 169
Size: 2563 184 1314
Fragments arranged by size:
2563 1314 184
MscI TGG'CCA
Cuts at: 304 487 664 1822 304
Size: 183 177 1158 2543
Fragments arranged by size:
2543 1158 183 177
MslI CAynn'nnrTG
Cuts at: 851 2050 2177 851
Size: 1199 127 2735
Fragments arranged by size:
2735 1199 127
MspA1I CmG'CkG
Cuts at: 798 1172 1486 1846 2859 3104 798
Size: 374 314 360 1013 245 1755
Fragments arranged by size:
1755 1013 374 360 314 245
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NsiI A_TGCA'T
Cuts at: 841 841
Size: 4061
NspI r_CATG'y
Cuts at: 511 839 1114 1614 2521 511
Size: 328 275 500 907 2051
Fragments arranged by size:
2051 907 500 328 275
PciI A'CATG_T
Cuts at: 2517 2517
Size: 4061
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 4029
PsiI TTA'TAA
Cuts at: 256 580 757 256
Size: 324 177 3560
Fragments arranged by size:
3560 324 177
PstI C_TGCA'G
Cuts at: 1108 1793 1108
Size: 685 3376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 798 1486 1846 798
Size: 688 360 3013
Fragments arranged by size:
3013 688 360
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 1075 1075
Size: 4061
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 629 862 1104 1789 2782 2973 33
Size: 596 233 242 685 993 191 1121
Fragments arranged by size:
1121 993 685 596 242 233 191
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 501 1216 2131 2623 2885 501
Size: 715 915 492 262 1677
Fragments arranged by size:
1677 915 715 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
SspI AAT'ATT
Cuts at: 752 752
Size: 4061
StuI AGG'CCT
Cuts at: 826 826
Size: 4061
StyI C'CwwG_G
Cuts at: 735 1195 1549 735
Size: 460 354 3247
Fragments arranged by size:
3247 460 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1644 1777 2236 2445 1644
Size: 133 459 209 3260
Fragments arranged by size:
3260 459 209 133
TatI w'GTAC_w
Cuts at: 290 3576 290
Size: 3286 775
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 539 571 3578 3610 539
Size: 32 3007 32 990
Fragments arranged by size:
3007 990 32 32
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4061
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 672 1556 672
Size: 884 3177
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
BaeI BbvCI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I BtgI ClaI DraIII
FseI FspAI HindIII HpaI NcoI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI ZraI