Restriction map for
pF1KB7367
(Circular) (Six-base) MAPSORT of: pF1KB7367.seq Check: 4922 from: 1 to: 3596
pF1KB7367 3596 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 155 197 634 155
Size: 42 437 3117
Fragments arranged by size:
3117 437 42
Acc65I G'GTAC_C
Cuts at: 182 612 182
Size: 430 3166
AclI AA'CG_TT
Cuts at: 3436 3436
Size: 3596
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1421 1853 2600 2868 412
Size: 1009 432 747 268 1140
Fragments arranged by size:
1140 1009 747 432 268
AflIII A'CryG_T
Cuts at: 948 2052 3029 948
Size: 1104 977 1515
Fragments arranged by size:
1515 1104 977
AgeI A'CCGG_T
Cuts at: 1984 1984
Size: 3596
AlwNI CAG_nnn'CTG
Cuts at: 450 901 2468 450
Size: 451 1567 1578
Fragments arranged by size:
1578 1567 451
ApaI G_GGCC'C
Cuts at: 293 362 399 293
Size: 69 37 3490
Fragments arranged by size:
3490 69 37
ApaLI G'TGCA_C
Cuts at: 313 2366 313
Size: 2053 1543
ApoI r'AATT_y
Cuts at: 600 2949 3205 600
Size: 2349 256 991
Fragments arranged by size:
2349 991 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3596
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3596
AvaI C'yCGr_G
Cuts at: 616 616
Size: 3596
BamHI G'GATC_C
Cuts at: 621 621
Size: 3596
BanI G'GyrC_C
Cuts at: 182 612 1026 1274 1309 182
Size: 430 414 248 35 2469
Fragments arranged by size:
2469 430 414 248 35
BanII G_rGCy'C
Cuts at: 293 362 399 610 1640 293
Size: 69 37 211 1030 2249
Fragments arranged by size:
2249 1030 211 69 37
BbeI G_GCGC'C
Cuts at: 1030 1278 1030
Size: 248 3348
BbsI GAAGACnn'nnnn_
Cuts at: 551 551
Size: 3596
BciVI GTATCCnnnnn_n'
Cuts at: 1494 2255 3596 1494
Size: 761 1341 1494
Fragments arranged by size:
1494 1341 761
BclI T'GATC_A
Cuts at: 1116 1116
Size: 3596
BglII A'GATC_T
Cuts at: 1111 3148 1111
Size: 2037 1559
BlpI GC'TnA_GC
Cuts at: 708 708
Size: 3596
Bme1580I G_kGCm'C
Cuts at: 293 317 362 399 1221 1314 2370 293
Size: 24 45 37 822 93 1056 1519
Fragments arranged by size:
1519 1056 822 93 45 37 24
BmrI ACTGGGnnnn_n'
Cuts at: 808 889 1224 3368 808
Size: 81 335 2144 1036
Fragments arranged by size:
2144 1036 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1963 1963
Size: 3596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 604
Size: 3596
Bpu10I CC'TnA_GC
Cuts at: 391 391
Size: 3596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1651 2143 2441 772
Size: 879 492 298 1927
Fragments arranged by size:
1927 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 494 580 3300 494
Size: 86 2720 790
Fragments arranged by size:
2720 790 86
BsaAI yAC'GTr
Cuts at: 201 949 201
Size: 748 2848
BsaBI GATnn'nnATC
Cuts at: 6 1110 3147 6
Size: 1104 2037 455
Fragments arranged by size:
2037 1104 455
BsaHI Gr'CG_yC
Cuts at: 152 1027 1275 1977 3473 152
Size: 875 248 702 1496 275
Fragments arranged by size:
1496 875 702 275 248
BsaWI w'CCGG_w
Cuts at: 785 1306 1984 2258 2405 2816 785
Size: 521 678 274 147 411 1565
Fragments arranged by size:
1565 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 433 433
Size: 3596
BseYI C'CCAG_C
Cuts at: 1021 1779 2356 2852 1021
Size: 758 577 496 1765
Fragments arranged by size:
1765 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1138 1184 2392 104
Size: 1034 46 1208 1308
Fragments arranged by size:
1308 1208 1034 46
BsiHKAI G_wGCw'C
Cuts at: 308 317 610 1388 1578 2370 308
Size: 9 293 778 190 792 1534
Fragments arranged by size:
1534 792 778 293 190 9
Bsp1286I G_dGCh'C
Cuts at: 293 308 317 362 399 610 1221 1314 1388
Size: 15 9 45 37 211 611 93 74
Cuts at: 1388 1578 1640 2370 293
Size: 190 62 730 1519
Fragments arranged by size:
1519 730 611 211 190 93 74 62
45 37 15 9
BspEI T'CCGG_A
Cuts at: 2816 2816
Size: 3596
BspHI T'CATG_A
Cuts at: 2772 2772
Size: 3596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 167 587 646 860 1162 1543 167
Size: 420 59 214 302 381 2220
Fragments arranged by size:
2220 420 381 302 214 59
BsrBI CCG'CTC
Cuts at: 50 1888 1942 3426 3591 50
Size: 1838 54 1484 165 55
Fragments arranged by size:
1838 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1508 1508
Size: 3596
BsrFI r'CCGG_y
Cuts at: 1594 1775 1984 1594
Size: 181 209 3206
Fragments arranged by size:
3206 209 181
BssSI C'ACGA_G
Cuts at: 1867 2225 1867
Size: 358 3238
BstAPI GCAn_nnn'nTGC
Cuts at: 450 973 450
Size: 523 3073
BstBI TT'CG_AA
Cuts at: 1957 1957
Size: 3596
BstYI r'GATC_y
Cuts at: 1 621 1088 1111 1446 1692 2704 2790 2802
Size: 620 467 23 335 246 1012 86 12
Cuts at: 2802 3148 1
Size: 346 449
Fragments arranged by size:
1012 620 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 116 332 107
Size: 9 216 3371
Fragments arranged by size:
3371 216 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1546 1727 1890 1546
Size: 181 163 3252
Fragments arranged by size:
3252 181 163
BtsI GCAGTG_nn'
Cuts at: 218 487 839 218
Size: 269 352 2975
Fragments arranged by size:
2975 352 269
DraI TTT'AAA
Cuts at: 595 595
Size: 3596
DrdI GACnn_nn'nnGTC
Cuts at: 1302 2160 1302
Size: 858 2738
EaeI y'GGCC_r
Cuts at: 335 1181 1355 1746 1773 3118 335
Size: 846 174 391 27 1345 813
Fragments arranged by size:
1345 846 813 391 174 27
EagI C'GGCC_G
Cuts at: 1181 1181
Size: 3596
EarI CTCTTCn'nnn_
Cuts at: 347 554 1619 1829 347
Size: 207 1065 210 2114
Fragments arranged by size:
2114 1065 210 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 288 1804 2114 2260 3406 288
Size: 1516 310 146 1146 478
Fragments arranged by size:
1516 1146 478 310 146
EcoICRI GAG'CTC
Cuts at: 608 608
Size: 3596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 604 1421 1853 2600 2868 412
Size: 192 817 432 747 268 1140
Fragments arranged by size:
1140 817 747 432 268 192
EcoNI CCTnn'n_nnAGG
Cuts at: 415 415
Size: 3596
EcoO109I rG'GnC_Cy
Cuts at: 112 266 289 290 358 359 395 735 112
Size: 154 23 1 68 1 36 340 2973
Fragments arranged by size:
2973 340 154 68 36 23 1 1
EcoRI G'AATT_C
Cuts at: 600 600
Size: 3596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1615 1615
Size: 3596
FspI TGC'GCA
Cuts at: 1377 1377
Size: 3596
HaeII r_GCGC'y
Cuts at: 144 1030 1278 2300 3279 144
Size: 886 248 1022 979 461
Fragments arranged by size:
1022 979 886 461 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 491 523 1438 1468 1470 1500 491
Size: 32 915 30 2 30 2587
Fragments arranged by size:
2587 915 32 30 30 2
HincII GTy'rAC
Cuts at: 216 635 216
Size: 419 3177
KasI G'GCGC_C
Cuts at: 1026 1274 1026
Size: 248 3348
KpnI G_GTAC'C
Cuts at: 186 616 186
Size: 430 3166
MluI A'CGCG_T
Cuts at: 3029 3029
Size: 3596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2267 2451 2267
Size: 184 3412
MscI TGG'CCA
Cuts at: 1357 1357
Size: 3596
MslI CAynn'nnrTG
Cuts at: 1585 1712 1585
Size: 127 3469
MspA1I CmG'CkG
Cuts at: 118 281 707 1021 1381 2394 2639 118
Size: 163 426 314 360 1013 245 1075
Fragments arranged by size:
1075 1013 426 360 314 245 163
NaeI GCC'GGC
Cuts at: 1777 1777
Size: 3596
NarI GG'CG_CC
Cuts at: 1027 1275 1027
Size: 248 3348
NcoI C'CATG_G
Cuts at: 107 107
Size: 3596
NgoMIV G'CCGG_C
Cuts at: 1775 1775
Size: 3596
NspI r_CATG'y
Cuts at: 649 1149 2056 649
Size: 500 907 2189
Fragments arranged by size:
2189 907 500
PasI CC'CwG_GG
Cuts at: 293 293
Size: 3596
PciI A'CATG_T
Cuts at: 2052 2052
Size: 3596
PmeI GTTT'AAAC
Cuts at: 595 595
Size: 3596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2735 2767 2735
Size: 32 3564
PpuMI rG'GwC_Cy
Cuts at: 112 112
Size: 3596
PspOMI G'GGCC_C
Cuts at: 289 358 395 289
Size: 69 37 3490
Fragments arranged by size:
3490 69 37
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 166 198 166
Size: 32 3564
PstI C_TGCA'G
Cuts at: 643 1328 643
Size: 685 2911
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3596
PvuII CAG'CTG
Cuts at: 1021 1381 1021
Size: 360 3236
RsrII CG'GwC_CG
Cuts at: 1791 1791
Size: 3596
SacI G_AGCT'C
Cuts at: 610 610
Size: 3596
SacII CC_GC'GG
Cuts at: 119 119
Size: 3596
SalI G'TCGA_C
Cuts at: 633 633
Size: 3596
SanDI GG'GwC_CC
Cuts at: 112 112
Size: 3596
SapI GCTCTTCn'nnn_
Cuts at: 1619 1829 1619
Size: 210 3386
SbfI CC_TGCA'GG
Cuts at: 643 643
Size: 3596
ScaI AGT'ACT
Cuts at: 3113 3113
Size: 3596
SfcI C'TryA_G
Cuts at: 33 639 1324 2317 2508 33
Size: 606 685 993 191 1121
Fragments arranged by size:
1121 993 685 606 191
SfoI GGC'GCC
Cuts at: 1028 1276 1028
Size: 248 3348
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3596
SmaI CCC'GGG
Cuts at: 618 618
Size: 3596
SmlI C'TyrA_G
Cuts at: 751 1666 2158 2420 751
Size: 915 492 262 1927
Fragments arranged by size:
1927 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3596
SphI G_CATG'C
Cuts at: 649 1149 649
Size: 500 3096
StyI C'CwwG_G
Cuts at: 107 730 1084 107
Size: 623 354 2619
Fragments arranged by size:
2619 623 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1179 1312 1771 1980 1179
Size: 133 459 209 2795
Fragments arranged by size:
2795 459 209 133
TatI w'GTAC_w
Cuts at: 3111 3111
Size: 3596
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3113 3145 3113
Size: 32 3564
Tth111I GACn'n_nGTC
Cuts at: 1393 1393
Size: 3596
XbaI T'CTAG_A
Cuts at: 627 627
Size: 3596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 241 1091 241
Size: 850 2746
XmaI C'CCGG_G
Cuts at: 616 616
Size: 3596
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PpuMI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PspXI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI