Restriction map for
pF1KB7366
(Circular) (Six-base) MAPSORT of: pF1KB7366.seq Check: 9639 from: 1 to: 3848
pF1KB7366 3848 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 400 568 400
Size: 168 3680
AccI GT'mk_AC
Cuts at: 886 886
Size: 3848
Acc65I G'GTAC_C
Cuts at: 864 864
Size: 3848
AclI AA'CG_TT
Cuts at: 3688 3688
Size: 3848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1673 2105 2852 3120 1673
Size: 432 747 268 2401
Fragments arranged by size:
2401 747 432 268
AfeI AGC'GCT
Cuts at: 529 529
Size: 3848
AflIII A'CryG_T
Cuts at: 1200 2304 3281 1200
Size: 1104 977 1767
Fragments arranged by size:
1767 1104 977
AgeI A'CCGG_T
Cuts at: 2236 2236
Size: 3848
AlwNI CAG_nnn'CTG
Cuts at: 138 284 327 1153 2720 138
Size: 146 43 826 1567 1266
Fragments arranged by size:
1567 1266 826 146 43
ApaI G_GGCC'C
Cuts at: 452 627 819 452
Size: 175 192 3481
Fragments arranged by size:
3481 192 175
ApaLI G'TGCA_C
Cuts at: 2618 2618
Size: 3848
ApoI r'AATT_y
Cuts at: 852 3201 3457 852
Size: 2349 256 1243
Fragments arranged by size:
2349 1243 256
AseI AT'TA_AT
Cuts at: 20 605 20
Size: 585 3263
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
AvaI C'yCGr_G
Cuts at: 253 868 253
Size: 615 3233
BamHI G'GATC_C
Cuts at: 455 873 455
Size: 418 3430
BanI G'GyrC_C
Cuts at: 864 1278 1526 1561 864
Size: 414 248 35 3151
Fragments arranged by size:
3151 414 248 35
BanII G_rGCy'C
Cuts at: 452 627 819 862 1892 452
Size: 175 192 43 1030 2408
Fragments arranged by size:
2408 1030 192 175 43
BbeI G_GCGC'C
Cuts at: 1282 1530 1282
Size: 248 3600
BciVI GTATCCnnnnn_n'
Cuts at: 1746 2507 3848 1746
Size: 761 1341 1746
Fragments arranged by size:
1746 1341 761
BclI T'GATC_A
Cuts at: 1368 1368
Size: 3848
BglII A'GATC_T
Cuts at: 1363 3400 1363
Size: 2037 1811
BlpI GC'TnA_GC
Cuts at: 960 960
Size: 3848
Bme1580I G_kGCm'C
Cuts at: 174 452 506 627 819 1473 1566 2622 174
Size: 278 54 121 192 654 93 1056 1400
Fragments arranged by size:
1400 1056 654 278 192 121 93 54
BmrI ACTGGGnnnn_n'
Cuts at: 372 1060 1141 1476 3620 372
Size: 688 81 335 2144 600
Fragments arranged by size:
2144 688 600 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 3848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 644 705 644
Size: 61 3787
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1903 2395 2693 1024
Size: 879 492 298 2179
Fragments arranged by size:
2179 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3552 3552
Size: 3848
BsaAI yAC'GTr
Cuts at: 1201 1201
Size: 3848
BsaBI GATnn'nnATC
Cuts at: 6 1362 3399 6
Size: 1356 2037 455
Fragments arranged by size:
2037 1356 455
BsaHI Gr'CG_yC
Cuts at: 1279 1527 2229 3725 1279
Size: 248 702 1496 1402
Fragments arranged by size:
1496 1402 702 248
BsaWI w'CCGG_w
Cuts at: 1037 1558 2236 2510 2657 3068 1037
Size: 521 678 274 147 411 1817
Fragments arranged by size:
1817 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 790 790
Size: 3848
BseYI C'CCAG_C
Cuts at: 173 247 811 1273 2031 2608 3104 173
Size: 74 564 462 758 577 496 917
Fragments arranged by size:
917 758 577 564 496 462 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 297 313 288
Size: 9 16 3823
Fragments arranged by size:
3823 16 9
BsiEI CG_ry'CG
Cuts at: 104 620 729 1390 1436 2644 104
Size: 516 109 661 46 1208 1308
Fragments arranged by size:
1308 1208 661 516 109 46
BsiHKAI G_wGCw'C
Cuts at: 436 862 1640 1830 2622 436
Size: 426 778 190 792 1662
Fragments arranged by size:
1662 792 778 426 190
BsmI GAATG_Cn'
Cuts at: 300 491 813 300
Size: 191 322 3335
Fragments arranged by size:
3335 322 191
BsmBI CGTCTCn'nnnn_
Cuts at: 827 827
Size: 3848
Bsp1286I G_dGCh'C
Cuts at: 174 436 452 506 627 819 862 1473 1566
Size: 262 16 54 121 192 43 611 93
Cuts at: 1566 1640 1830 1892 2622 174
Size: 74 190 62 730 1400
Fragments arranged by size:
1400 730 611 262 192 190 121 93
74 62 54 43 16
BspEI T'CCGG_A
Cuts at: 3068 3068
Size: 3848
BspHI T'CATG_A
Cuts at: 3024 3024
Size: 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 212 400 453 568 898 1112 1414 1795 212
Size: 188 53 115 330 214 302 381 2265
Fragments arranged by size:
2265 381 330 302 214 188 115 53
BsrBI CCG'CTC
Cuts at: 50 725 2140 2194 3678 3843 50
Size: 675 1415 54 1484 165 55
Fragments arranged by size:
1484 1415 675 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1760 1760
Size: 3848
BsrFI r'CCGG_y
Cuts at: 1846 2027 2236 1846
Size: 181 209 3458
Fragments arranged by size:
3458 209 181
BsrGI T'GTAC_A
Cuts at: 350 350
Size: 3848
BssSI C'ACGA_G
Cuts at: 824 2119 2477 824
Size: 1295 358 2195
Fragments arranged by size:
2195 1295 358
BstAPI GCAn_nnn'nTGC
Cuts at: 129 138 1225 129
Size: 9 1087 2752
Fragments arranged by size:
2752 1087 9
BstBI TT'CG_AA
Cuts at: 2209 2209
Size: 3848
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3848
BstYI r'GATC_y
Cuts at: 1 455 873 1340 1363 1698 1944 2956 3042
Size: 454 418 467 23 335 246 1012 86
Cuts at: 3042 3054 3400 1
Size: 12 346 449
Fragments arranged by size:
1012 467 454 449 418 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 597 597
Size: 3848
BtgI C'CryG_G
Cuts at: 107 107
Size: 3848
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1798 1979 2142 1798
Size: 181 163 3504
Fragments arranged by size:
3504 181 163
BtsI GCAGTG_nn'
Cuts at: 1091 1091
Size: 3848
DraI TTT'AAA
Cuts at: 847 847
Size: 3848
DrdI GACnn_nn'nnGTC
Cuts at: 1554 2412 1554
Size: 858 2990
EaeI y'GGCC_r
Cuts at: 270 308 617 726 782 800 1433 1607 1998
Size: 38 309 109 56 18 633 174 391
Cuts at: 1998 2025 3370 270
Size: 27 1345 748
Fragments arranged by size:
1345 748 633 391 309 174 109 56
38 27 18
EagI C'GGCC_G
Cuts at: 617 726 1433 617
Size: 109 707 3032
Fragments arranged by size:
3032 707 109
EarI CTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2056 2366 2512 3658 2056
Size: 310 146 1146 2246
Fragments arranged by size:
2246 1146 310 146
EcoICRI GAG'CTC
Cuts at: 860 860
Size: 3848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 644 705 1673 2105 2852 3120 644
Size: 61 968 432 747 268 1372
Fragments arranged by size:
1372 968 747 432 268 61
EcoO109I rG'GnC_Cy
Cuts at: 448 449 623 624 815 816 987 448
Size: 1 174 1 191 1 171 3309
Fragments arranged by size:
3309 191 174 171 1 1 1
EcoRI G'AATT_C
Cuts at: 852 852
Size: 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 420 1867 420
Size: 1447 2401
FspI TGC'GCA
Cuts at: 1629 1629
Size: 3848
HaeII r_GCGC'y
Cuts at: 402 531 1282 1530 2552 3531 402
Size: 129 751 248 1022 979 719
Fragments arranged by size:
1022 979 751 719 248 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1690 1720 1722 1752 1690
Size: 30 2 30 3786
Fragments arranged by size:
3786 30 30 2
HincII GTy'rAC
Cuts at: 468 887 468
Size: 419 3429
KasI G'GCGC_C
Cuts at: 1278 1526 1278
Size: 248 3600
KpnI G_GTAC'C
Cuts at: 868 868
Size: 3848
MluI A'CGCG_T
Cuts at: 3281 3281
Size: 3848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2519 2703 2519
Size: 184 3664
MscI TGG'CCA
Cuts at: 272 784 1609 272
Size: 512 825 2511
Fragments arranged by size:
2511 825 512
MslI CAynn'nnrTG
Cuts at: 112 739 1837 1964 112
Size: 627 1098 127 1996
Fragments arranged by size:
1996 1098 627 127
MspA1I CmG'CkG
Cuts at: 135 419 731 959 1273 1633 2646 2891 135
Size: 284 312 228 314 360 1013 245 1092
Fragments arranged by size:
1092 1013 360 314 312 284 245 228
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 3848
NarI GG'CG_CC
Cuts at: 1279 1527 1279
Size: 248 3600
NcoI C'CATG_G
Cuts at: 107 107
Size: 3848
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 3848
NotI GC'GGCC_GC
Cuts at: 726 726
Size: 3848
NspI r_CATG'y
Cuts at: 901 1401 2308 901
Size: 500 907 2441
Fragments arranged by size:
2441 907 500
PasI CC'CwG_GG
Cuts at: 759 759
Size: 3848
PciI A'CATG_T
Cuts at: 2304 2304
Size: 3848
PfoI T'CCnGG_A
Cuts at: 410 410
Size: 3848
PmeI GTTT'AAAC
Cuts at: 847 847
Size: 3848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 3816
PshAI GACnn'nnGTC
Cuts at: 419 419
Size: 3848
PspOMI G'GGCC_C
Cuts at: 448 623 815 448
Size: 175 192 3481
Fragments arranged by size:
3481 192 175
PstI C_TGCA'G
Cuts at: 634 895 1580 634
Size: 261 685 2902
Fragments arranged by size:
2902 685 261
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3848
PvuII CAG'CTG
Cuts at: 135 419 1273 1633 135
Size: 284 854 360 2350
Fragments arranged by size:
2350 854 360 284
RsrII CG'GwC_CG
Cuts at: 2043 2043
Size: 3848
SacI G_AGCT'C
Cuts at: 862 862
Size: 3848
SalI G'TCGA_C
Cuts at: 885 885
Size: 3848
SapI GCTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
SbfI CC_TGCA'GG
Cuts at: 895 895
Size: 3848
ScaI AGT'ACT
Cuts at: 3365 3365
Size: 3848
SfcI C'TryA_G
Cuts at: 33 630 711 891 1576 2569 2760 33
Size: 597 81 180 685 993 191 1121
Fragments arranged by size:
1121 993 685 597 191 180 81
SfoI GGC'GCC
Cuts at: 1280 1528 1280
Size: 248 3600
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
SmaI CCC'GGG
Cuts at: 870 870
Size: 3848
SmlI C'TyrA_G
Cuts at: 1003 1918 2410 2672 1003
Size: 915 492 262 2179
Fragments arranged by size:
2179 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3848
SphI G_CATG'C
Cuts at: 901 1401 901
Size: 500 3348
StuI AGG'CCT
Cuts at: 680 680
Size: 3848
StyI C'CwwG_G
Cuts at: 107 982 1336 107
Size: 875 354 2619
Fragments arranged by size:
2619 875 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 453 1431 1564 2023 2232 453
Size: 978 133 459 209 2069
Fragments arranged by size:
2069 978 459 209 133
TatI w'GTAC_w
Cuts at: 350 3363 350
Size: 3013 835
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 3816
Tth111I GACn'n_nGTC
Cuts at: 1645 1645
Size: 3848
XbaI T'CTAG_A
Cuts at: 879 879
Size: 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 777 1343 777
Size: 566 3282
XmaI C'CCGG_G
Cuts at: 868 868
Size: 3848
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PfoI
PmeI PpiI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PflMI PmlI PpuMI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI