Restriction map for
pF1KB7363
(Circular) (Six-base) MAPSORT of: pF1KB7363.seq Check: 702 from: 1 to: 4199
pF1KB7363 4199 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1237 1237
Size: 4199
Acc65I G'GTAC_C
Cuts at: 1187 1215 1187
Size: 28 4171
AclI AA'CG_TT
Cuts at: 4039 4039
Size: 4199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2024 2456 3203 3471 2024
Size: 432 747 268 2752
Fragments arranged by size:
2752 747 432 268
AflIII A'CryG_T
Cuts at: 1551 2655 3632 1551
Size: 1104 977 2118
Fragments arranged by size:
2118 1104 977
AgeI A'CCGG_T
Cuts at: 2587 2587
Size: 4199
AleI CACnn'nnGTG
Cuts at: 469 469
Size: 4199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 769 769
Size: 4199
AlwNI CAG_nnn'CTG
Cuts at: 949 1504 3071 949
Size: 555 1567 2077
Fragments arranged by size:
2077 1567 555
ApaI G_GGCC'C
Cuts at: 1159 1159
Size: 4199
ApaLI G'TGCA_C
Cuts at: 2969 2969
Size: 4199
ApoI r'AATT_y
Cuts at: 611 841 1203 3552 3808 611
Size: 230 362 2349 256 1002
Fragments arranged by size:
2349 1002 362 256 230
AseI AT'TA_AT
Cuts at: 20 20
Size: 4199
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
AvaI C'yCGr_G
Cuts at: 695 707 867 886 1086 1219 695
Size: 12 160 19 200 133 3675
Fragments arranged by size:
3675 200 160 133 19 12
BamHI G'GATC_C
Cuts at: 1224 1224
Size: 4199
BanI G'GyrC_C
Cuts at: 551 1187 1215 1629 1877 1912 551
Size: 636 28 414 248 35 2838
Fragments arranged by size:
2838 636 414 248 35 28
BanII G_rGCy'C
Cuts at: 362 691 1064 1159 1213 2243 362
Size: 329 373 95 54 1030 2318
Fragments arranged by size:
2318 1030 373 329 95 54
BbeI G_GCGC'C
Cuts at: 1633 1881 1633
Size: 248 3951
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1119 1153 1119
Size: 34 4165
BciVI GTATCCnnnnn_n'
Cuts at: 2097 2858 4199 2097
Size: 761 1341 2097
Fragments arranged by size:
2097 1341 761
BclI T'GATC_A
Cuts at: 1719 1719
Size: 4199
BglII A'GATC_T
Cuts at: 437 1714 3751 437
Size: 1277 2037 885
Fragments arranged by size:
2037 1277 885
BlpI GC'TnA_GC
Cuts at: 659 690 1311 659
Size: 31 621 3547
Fragments arranged by size:
3547 621 31
Bme1580I G_kGCm'C
Cuts at: 1159 1824 1917 2973 1159
Size: 665 93 1056 2385
Fragments arranged by size:
2385 1056 665 93
BmgBI CAC'GTC
Cuts at: 480 480
Size: 4199
BmrI ACTGGGnnnn_n'
Cuts at: 871 1411 1492 1827 3971 871
Size: 540 81 335 2144 1099
Fragments arranged by size:
2144 1099 540 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 381 2566 381
Size: 2185 2014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 980 980
Size: 4199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1375 2254 2746 3044 1375
Size: 879 492 298 2530
Fragments arranged by size:
2530 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 710 3903 710
Size: 3193 1006
BsaAI yAC'GTr
Cuts at: 1552 1552
Size: 4199
BsaBI GATnn'nnATC
Cuts at: 6 175 1713 3750 6
Size: 169 1538 2037 455
Fragments arranged by size:
2037 1538 455 169
BsaHI Gr'CG_yC
Cuts at: 518 731 1630 1878 2580 4076 518
Size: 213 899 248 702 1496 641
Fragments arranged by size:
1496 899 702 641 248 213
BsaWI w'CCGG_w
Cuts at: 1388 1909 2587 2861 3008 3419 1388
Size: 521 678 274 147 411 2168
Fragments arranged by size:
2168 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 589 619 661 691 972 1002 589
Size: 30 42 30 281 30 3786
Fragments arranged by size:
3786 281 42 30 30 30
BseYI C'CCAG_C
Cuts at: 125 741 756 1009 1126 1624 2382 2959 3455
Size: 616 15 253 117 498 758 577 496
Cuts at: 3455 125
Size: 869
Fragments arranged by size:
869 758 616 577 498 496 253 117
15
BsiEI CG_ry'CG
Cuts at: 104 1741 1787 2995 104
Size: 1637 46 1208 1308
Fragments arranged by size:
1637 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 362 408 1064 1213 1991 2181 2973 362
Size: 46 656 149 778 190 792 1588
Fragments arranged by size:
1588 792 778 656 190 149 46
Bsp1286I G_dGCh'C
Cuts at: 362 408 691 1064 1159 1213 1824 1917 1991
Size: 46 283 373 95 54 611 93 74
Cuts at: 1991 2181 2243 2973 362
Size: 190 62 730 1588
Fragments arranged by size:
1588 730 611 373 283 190 95 93
74 62 54 46
BspEI T'CCGG_A
Cuts at: 3419 3419
Size: 4199
BspHI T'CATG_A
Cuts at: 621 3375 621
Size: 2754 1445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1249 1463 1765 2146 1249
Size: 214 302 381 3302
Fragments arranged by size:
3302 381 302 214
BsrBI CCG'CTC
Cuts at: 50 855 2491 2545 4029 4194 50
Size: 805 1636 54 1484 165 55
Fragments arranged by size:
1636 1484 805 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2111 2111
Size: 4199
BsrFI r'CCGG_y
Cuts at: 548 1132 2197 2378 2587 548
Size: 584 1065 181 209 2160
Fragments arranged by size:
2160 1065 584 209 181
BssSI C'ACGA_G
Cuts at: 355 2470 2828 355
Size: 2115 358 1726
Fragments arranged by size:
2115 1726 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1576 1576
Size: 4199
BstBI TT'CG_AA
Cuts at: 2560 2560
Size: 4199
BstEII G'GTnAC_C
Cuts at: 760 760
Size: 4199
BstXI CCAn_nnnn'nTGG
Cuts at: 1150 1150
Size: 4199
BstYI r'GATC_y
Cuts at: 1 283 437 1224 1691 1714 2049 2295 3307
Size: 282 154 787 467 23 335 246 1012
Cuts at: 3307 3393 3405 3751 1
Size: 86 12 346 449
Fragments arranged by size:
1012 787 467 449 346 335 282 246
154 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 383 383
Size: 4199
BtgI C'CryG_G
Cuts at: 107 467 1052 107
Size: 360 585 3254
Fragments arranged by size:
3254 585 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2149 2330 2493 2149
Size: 181 163 3855
Fragments arranged by size:
3855 181 163
BtsI GCAGTG_nn'
Cuts at: 908 1442 908
Size: 534 3665
DraI TTT'AAA
Cuts at: 1198 1198
Size: 4199
DrdI GACnn_nn'nnGTC
Cuts at: 1090 1905 2763 1090
Size: 815 858 2526
Fragments arranged by size:
2526 858 815
EaeI y'GGCC_r
Cuts at: 228 1055 1073 1140 1784 1958 2349 2376 3721
Size: 827 18 67 644 174 391 27 1345
Cuts at: 3721 228
Size: 706
Fragments arranged by size:
1345 827 706 644 391 174 67 27
18
EagI C'GGCC_G
Cuts at: 1784 1784
Size: 4199
EarI CTCTTCn'nnn_
Cuts at: 2222 2432 2222
Size: 210 3989
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2407 2717 2863 4009 2407
Size: 310 146 1146 2597
Fragments arranged by size:
2597 1146 310 146
EcoICRI GAG'CTC
Cuts at: 360 1062 1211 360
Size: 702 149 3348
Fragments arranged by size:
3348 702 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 980 2024 2456 3203 3471 980
Size: 1044 432 747 268 1708
Fragments arranged by size:
1708 1044 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 267 1155 1156 1338 267
Size: 888 1 182 3128
Fragments arranged by size:
3128 888 182 1
EcoRI G'AATT_C
Cuts at: 841 1203 841
Size: 362 3837
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 408 456 2218 408
Size: 48 1762 2389
Fragments arranged by size:
2389 1762 48
FspI TGC'GCA
Cuts at: 1980 1980
Size: 4199
HaeII r_GCGC'y
Cuts at: 875 1112 1633 1881 2903 3882 875
Size: 237 521 248 1022 979 1192
Fragments arranged by size:
1192 1022 979 521 248 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 408 440 661 693 954 972 986 1004 2041
Size: 32 221 32 261 18 14 18 1037
Cuts at: 2041 2071 2073 2103 408
Size: 30 2 30 2504
Fragments arranged by size:
2504 1037 261 221 32 32 30 30
18 18 14 2
HincII GTy'rAC
Cuts at: 1238 1238
Size: 4199
KasI G'GCGC_C
Cuts at: 1629 1877 1629
Size: 248 3951
KpnI G_GTAC'C
Cuts at: 1191 1219 1191
Size: 28 4171
MfeI C'AATT_G
Cuts at: 374 374
Size: 4199
MluI A'CGCG_T
Cuts at: 3632 3632
Size: 4199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2870 3054 2870
Size: 184 4015
MscI TGG'CCA
Cuts at: 1960 1960
Size: 4199
MslI CAynn'nnrTG
Cuts at: 310 469 526 620 751 2188 2315 310
Size: 159 57 94 131 1437 127 2194
Fragments arranged by size:
2194 1437 159 131 127 94 57
MspA1I CmG'CkG
Cuts at: 937 946 1310 1624 1984 2997 3242 937
Size: 9 364 314 360 1013 245 1894
Fragments arranged by size:
1894 1013 364 360 314 245 9
NaeI GCC'GGC
Cuts at: 2380 2380
Size: 4199
NarI GG'CG_CC
Cuts at: 1630 1878 1630
Size: 248 3951
NcoI C'CATG_G
Cuts at: 107 1052 107
Size: 945 3254
NgoMIV G'CCGG_C
Cuts at: 2378 2378
Size: 4199
NspI r_CATG'y
Cuts at: 756 1252 1752 2659 756
Size: 496 500 907 2296
Fragments arranged by size:
2296 907 500 496
PciI A'CATG_T
Cuts at: 2655 2655
Size: 4199
PmeI GTTT'AAAC
Cuts at: 1198 1198
Size: 4199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 3338
Size: 32 4167
PpuMI rG'GwC_Cy
Cuts at: 267 267
Size: 4199
PspOMI G'GGCC_C
Cuts at: 1155 1155
Size: 4199
PstI C_TGCA'G
Cuts at: 1246 1931 1246
Size: 685 3514
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4199
PvuII CAG'CTG
Cuts at: 1624 1984 1624
Size: 360 3839
RsrII CG'GwC_CG
Cuts at: 2394 2394
Size: 4199
SacI G_AGCT'C
Cuts at: 362 1064 1213 362
Size: 702 149 3348
Fragments arranged by size:
3348 702 149
SalI G'TCGA_C
Cuts at: 1236 1236
Size: 4199
SanDI GG'GwC_CC
Cuts at: 267 267
Size: 4199
SapI GCTCTTCn'nnn_
Cuts at: 2222 2432 2222
Size: 210 3989
SbfI CC_TGCA'GG
Cuts at: 1246 1246
Size: 4199
ScaI AGT'ACT
Cuts at: 3716 3716
Size: 4199
SfcI C'TryA_G
Cuts at: 33 1242 1927 2920 3111 33
Size: 1209 685 993 191 1121
Fragments arranged by size:
1209 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1631 1879 1631
Size: 248 3951
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
SmaI CCC'GGG
Cuts at: 697 1221 697
Size: 524 3675
SmlI C'TyrA_G
Cuts at: 707 1354 2269 2761 3023 707
Size: 647 915 492 262 1883
Fragments arranged by size:
1883 915 647 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4199
SphI G_CATG'C
Cuts at: 756 1252 1752 756
Size: 496 500 3203
Fragments arranged by size:
3203 500 496
SspI AAT'ATT
Cuts at: 490 490
Size: 4199
StuI AGG'CCT
Cuts at: 809 809
Size: 4199
StyI C'CwwG_G
Cuts at: 107 1052 1333 1687 107
Size: 945 281 354 2619
Fragments arranged by size:
2619 945 354 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1782 1915 2374 2583 1782
Size: 133 459 209 3398
Fragments arranged by size:
3398 459 209 133
TatI w'GTAC_w
Cuts at: 3714 3714
Size: 4199
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 589 621 3716 3748 589
Size: 32 3095 32 1040
Fragments arranged by size:
3095 1040 32 32
Tth111I GACn'n_nGTC
Cuts at: 1996 1996
Size: 4199
XbaI T'CTAG_A
Cuts at: 1230 1230
Size: 4199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1694 1694
Size: 4199
XhoI C'TCGA_G
Cuts at: 707 707
Size: 4199
XmaI C'CCGG_G
Cuts at: 695 1219 695
Size: 524 3675
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmtI Bpu10I BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SwaI XmnI ZraI