Restriction map for
pF1KB7362
(Circular) (Six-base) MAPSORT of: pF1KB7362.seq Check: 7091 from: 1 to: 3869
pF1KB7362 3869 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 907 907
Size: 3869
Acc65I G'GTAC_C
Cuts at: 885 885
Size: 3869
AclI AA'CG_TT
Cuts at: 3709 3709
Size: 3869
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1694 2126 2873 3141 1694
Size: 432 747 268 2422
Fragments arranged by size:
2422 747 432 268
AflII C'TTAA_G
Cuts at: 651 651
Size: 3869
AflIII A'CryG_T
Cuts at: 1221 2325 3302 1221
Size: 1104 977 1788
Fragments arranged by size:
1788 1104 977
AgeI A'CCGG_T
Cuts at: 2257 2257
Size: 3869
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 178 210 178
Size: 32 3837
AlwNI CAG_nnn'CTG
Cuts at: 1174 2741 1174
Size: 1567 2302
ApaI G_GGCC'C
Cuts at: 215 246 215
Size: 31 3838
ApaLI G'TGCA_C
Cuts at: 2639 2639
Size: 3869
ApoI r'AATT_y
Cuts at: 873 3222 3478 873
Size: 2349 256 1264
Fragments arranged by size:
2349 1264 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3869
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
AvaI C'yCGr_G
Cuts at: 166 199 889 166
Size: 33 690 3146
Fragments arranged by size:
3146 690 33
BamHI G'GATC_C
Cuts at: 894 894
Size: 3869
BanI G'GyrC_C
Cuts at: 205 607 885 1299 1547 1582 205
Size: 402 278 414 248 35 2492
Fragments arranged by size:
2492 414 402 278 248 35
BanII G_rGCy'C
Cuts at: 215 246 284 883 1913 215
Size: 31 38 599 1030 2171
Fragments arranged by size:
2171 1030 599 38 31
BbeI G_GCGC'C
Cuts at: 209 1303 1551 209
Size: 1094 248 2527
Fragments arranged by size:
2527 1094 248
BbsI GAAGACnn'nnnn_
Cuts at: 749 841 749
Size: 92 3777
BciVI GTATCCnnnnn_n'
Cuts at: 1767 2528 3869 1767
Size: 761 1341 1767
Fragments arranged by size:
1767 1341 761
BclI T'GATC_A
Cuts at: 1389 1389
Size: 3869
BfrBI ATG'CAT
Cuts at: 851 851
Size: 3869
BglII A'GATC_T
Cuts at: 345 1384 3421 345
Size: 1039 2037 793
Fragments arranged by size:
2037 1039 793
BlpI GC'TnA_GC
Cuts at: 981 981
Size: 3869
Bme1580I G_kGCm'C
Cuts at: 215 246 643 1494 1587 2643 215
Size: 31 397 851 93 1056 1441
Fragments arranged by size:
1441 1056 851 397 93 31
BmrI ACTGGGnnnn_n'
Cuts at: 1081 1162 1497 3641 1081
Size: 81 335 2144 1309
Fragments arranged by size:
2144 1309 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 3869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 792 792
Size: 3869
Bpu10I CC'TnA_GC
Cuts at: 375 375
Size: 3869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1924 2416 2714 1045
Size: 879 492 298 2200
Fragments arranged by size:
2200 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3573 3573
Size: 3869
BsaAI yAC'GTr
Cuts at: 219 1222 219
Size: 1003 2866
BsaBI GATnn'nnATC
Cuts at: 6 1383 3420 6
Size: 1377 2037 455
Fragments arranged by size:
2037 1377 455
BsaHI Gr'CG_yC
Cuts at: 206 1300 1548 2250 3746 206
Size: 1094 248 702 1496 329
Fragments arranged by size:
1496 1094 702 329 248
BsaWI w'CCGG_w
Cuts at: 1058 1579 2257 2531 2678 3089 1058
Size: 521 678 274 147 411 1838
Fragments arranged by size:
1838 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 687 1294 2052 2629 3125 687
Size: 607 758 577 496 1431
Fragments arranged by size:
1431 758 607 577 496
BsiEI CG_ry'CG
Cuts at: 104 1411 1457 2665 104
Size: 1307 46 1208 1308
Fragments arranged by size:
1308 1307 1208 46
BsiHKAI G_wGCw'C
Cuts at: 443 544 710 883 1661 1851 2643 443
Size: 101 166 173 778 190 792 1669
Fragments arranged by size:
1669 792 778 190 173 166 101
Bsp1286I G_dGCh'C
Cuts at: 215 246 284 443 544 643 710 883 1494
Size: 31 38 159 101 99 67 173 611
Cuts at: 1494 1587 1661 1851 1913 2643 215
Size: 93 74 190 62 730 1441
Fragments arranged by size:
1441 730 611 190 173 159 101 99
93 74 67 62 38 31
BspEI T'CCGG_A
Cuts at: 3089 3089
Size: 3869
BspHI T'CATG_A
Cuts at: 3045 3045
Size: 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 919 1133 1435 1816 919
Size: 214 302 381 2972
Fragments arranged by size:
2972 381 302 214
BsrBI CCG'CTC
Cuts at: 50 468 2161 2215 3699 3864 50
Size: 418 1693 54 1484 165 55
Fragments arranged by size:
1693 1484 418 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1781 1781
Size: 3869
BsrFI r'CCGG_y
Cuts at: 177 1867 2048 2257 177
Size: 1690 181 209 1789
Fragments arranged by size:
1789 1690 209 181
BssHII G'CGCG_C
Cuts at: 114 116 114
Size: 2 3867
BssSI C'ACGA_G
Cuts at: 2140 2498 2140
Size: 358 3511
BstAPI GCAn_nnn'nTGC
Cuts at: 1246 1246
Size: 3869
BstBI TT'CG_AA
Cuts at: 2230 2230
Size: 3869
BstXI CCAn_nnnn'nTGG
Cuts at: 687 687
Size: 3869
BstYI r'GATC_y
Cuts at: 1 345 894 1361 1384 1719 1965 2977 3063
Size: 344 549 467 23 335 246 1012 86
Cuts at: 3063 3075 3421 1
Size: 12 346 449
Fragments arranged by size:
1012 549 467 449 346 344 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 159 407 450 549 159
Size: 248 43 99 3479
Fragments arranged by size:
3479 248 99 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1819 2000 2163 1819
Size: 181 163 3525
Fragments arranged by size:
3525 181 163
BtsI GCAGTG_nn'
Cuts at: 418 1112 418
Size: 694 3175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 664 699 664
Size: 35 3834
DraI TTT'AAA
Cuts at: 868 868
Size: 3869
DrdI GACnn_nn'nnGTC
Cuts at: 1575 2433 1575
Size: 858 3011
EaeI y'GGCC_r
Cuts at: 1454 1628 2019 2046 3391 1454
Size: 174 391 27 1345 1932
Fragments arranged by size:
1932 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1454 1454
Size: 3869
EarI CTCTTCn'nnn_
Cuts at: 479 1892 2102 479
Size: 1413 210 2246
Fragments arranged by size:
2246 1413 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 577 2077 2387 2533 3679 577
Size: 1500 310 146 1146 767
Fragments arranged by size:
1500 1146 767 310 146
EcoICRI GAG'CTC
Cuts at: 881 881
Size: 3869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 792 1694 2126 2873 3141 792
Size: 902 432 747 268 1520
Fragments arranged by size:
1520 902 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 229 229
Size: 3869
EcoO109I rG'GnC_Cy
Cuts at: 212 1008 212
Size: 796 3073
EcoRI G'AATT_C
Cuts at: 873 873
Size: 3869
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1888 1888
Size: 3869
FspI TGC'GCA
Cuts at: 1650 1650
Size: 3869
HaeII r_GCGC'y
Cuts at: 159 209 571 1303 1551 2573 3552 159
Size: 50 362 732 248 1022 979 476
Fragments arranged by size:
1022 979 732 476 362 248 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1711 1741 1743 1773 1711
Size: 30 2 30 3807
Fragments arranged by size:
3807 30 30 2
HincII GTy'rAC
Cuts at: 908 908
Size: 3869
KasI G'GCGC_C
Cuts at: 205 1299 1547 205
Size: 1094 248 2527
Fragments arranged by size:
2527 1094 248
KpnI G_GTAC'C
Cuts at: 889 889
Size: 3869
MluI A'CGCG_T
Cuts at: 3302 3302
Size: 3869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 416 461 2540 2724 416
Size: 45 2079 184 1561
Fragments arranged by size:
2079 1561 184 45
MscI TGG'CCA
Cuts at: 1630 1630
Size: 3869
MslI CAynn'nnrTG
Cuts at: 846 856 1858 1985 846
Size: 10 1002 127 2730
Fragments arranged by size:
2730 1002 127 10
MspA1I CmG'CkG
Cuts at: 161 600 980 1294 1654 2667 2912 161
Size: 439 380 314 360 1013 245 1118
Fragments arranged by size:
1118 1013 439 380 360 314 245
NaeI GCC'GGC
Cuts at: 179 2050 179
Size: 1871 1998
NarI GG'CG_CC
Cuts at: 206 1300 1548 206
Size: 1094 248 2527
Fragments arranged by size:
2527 1094 248
NcoI C'CATG_G
Cuts at: 407 549 407
Size: 142 3727
NgoMIV G'CCGG_C
Cuts at: 177 2048 177
Size: 1871 1998
NsiI A_TGCA'T
Cuts at: 853 853
Size: 3869
NspI r_CATG'y
Cuts at: 922 1422 2329 922
Size: 500 907 2462
Fragments arranged by size:
2462 907 500
PciI A'CATG_T
Cuts at: 2325 2325
Size: 3869
PmeI GTTT'AAAC
Cuts at: 868 868
Size: 3869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 3837
PspOMI G'GGCC_C
Cuts at: 211 242 211
Size: 31 3838
PstI C_TGCA'G
Cuts at: 425 916 1601 425
Size: 491 685 2693
Fragments arranged by size:
2693 685 491
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3869
PvuII CAG'CTG
Cuts at: 1294 1654 1294
Size: 360 3509
RsrII CG'GwC_CG
Cuts at: 2064 2064
Size: 3869
SacI G_AGCT'C
Cuts at: 883 883
Size: 3869
SacII CC_GC'GG
Cuts at: 162 162
Size: 3869
SalI G'TCGA_C
Cuts at: 906 906
Size: 3869
SapI GCTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
SbfI CC_TGCA'GG
Cuts at: 916 916
Size: 3869
ScaI AGT'ACT
Cuts at: 3386 3386
Size: 3869
SfcI C'TryA_G
Cuts at: 33 421 912 1597 2590 2781 33
Size: 388 491 685 993 191 1121
Fragments arranged by size:
1121 993 685 491 388 191
SfoI GGC'GCC
Cuts at: 207 1301 1549 207
Size: 1094 248 2527
Fragments arranged by size:
2527 1094 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
SmaI CCC'GGG
Cuts at: 891 891
Size: 3869
SmlI C'TyrA_G
Cuts at: 651 1024 1939 2431 2693 651
Size: 373 915 492 262 1827
Fragments arranged by size:
1827 915 492 373 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3869
SphI G_CATG'C
Cuts at: 922 1422 922
Size: 500 3369
StyI C'CwwG_G
Cuts at: 407 549 1003 1357 407
Size: 142 454 354 2919
Fragments arranged by size:
2919 454 354 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1452 1585 2044 2253 1452
Size: 133 459 209 3068
Fragments arranged by size:
3068 459 209 133
TatI w'GTAC_w
Cuts at: 3384 3384
Size: 3869
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3386 3418 3386
Size: 32 3837
Tth111I GACn'n_nGTC
Cuts at: 1666 1666
Size: 3869
XbaI T'CTAG_A
Cuts at: 699 900 699
Size: 201 3668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1364 1364
Size: 3869
XmaI C'CCGG_G
Cuts at: 889 889
Size: 3869
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI BsaXI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI