Restriction map for
pF1KB7358
(Circular) (Six-base) MAPSORT of: pF1KB7358.seq Check: 359 from: 1 to: 4370
pF1KB7358 4370 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1408 1408
Size: 4370
Acc65I G'GTAC_C
Cuts at: 185 1386 185
Size: 1201 3169
AclI AA'CG_TT
Cuts at: 4210 4210
Size: 4370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 955 989 2195 2627 3374 3642 713
Size: 242 34 1206 432 747 268 1441
Fragments arranged by size:
1441 1206 747 432 268 242 34
AflIII A'CryG_T
Cuts at: 1722 2826 3803 1722
Size: 1104 977 2289
Fragments arranged by size:
2289 1104 977
AgeI A'CCGG_T
Cuts at: 2758 2758
Size: 4370
AlwNI CAG_nnn'CTG
Cuts at: 1675 3242 1675
Size: 1567 2803
ApaLI G'TGCA_C
Cuts at: 3140 3140
Size: 4370
ApoI r'AATT_y
Cuts at: 1374 3723 3979 1374
Size: 2349 256 1765
Fragments arranged by size:
2349 1765 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 1390 1390
Size: 4370
BamHI G'GATC_C
Cuts at: 1395 1395
Size: 4370
BanI G'GyrC_C
Cuts at: 185 1386 1800 2048 2083 185
Size: 1201 414 248 35 2472
Fragments arranged by size:
2472 1201 414 248 35
BanII G_rGCy'C
Cuts at: 1384 2414 1384
Size: 1030 3340
BbeI G_GCGC'C
Cuts at: 1804 2052 1804
Size: 248 4122
BbsI GAAGACnn'nnnn_
Cuts at: 1135 1135
Size: 4370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4336
BciVI GTATCCnnnnn_n'
Cuts at: 562 2268 3029 4370 562
Size: 1706 761 1341 562
Fragments arranged by size:
1706 1341 761 562
BclI T'GATC_A
Cuts at: 1890 1890
Size: 4370
BfrBI ATG'CAT
Cuts at: 470 470
Size: 4370
BglI GCCn_nnn'nGGC
Cuts at: 112 254 112
Size: 142 4228
BglII A'GATC_T
Cuts at: 1885 3922 1885
Size: 2037 2333
BlpI GC'TnA_GC
Cuts at: 865 1482 865
Size: 617 3753
Bme1580I G_kGCm'C
Cuts at: 1995 2088 3144 1995
Size: 93 1056 3221
Fragments arranged by size:
3221 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1582 1663 1998 4142 1582
Size: 81 335 2144 1810
Fragments arranged by size:
2144 1810 335 81
BmtI G_CTAG'C
Cuts at: 605 605
Size: 4370
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 231 795 1148 123
Size: 108 564 353 3345
Fragments arranged by size:
3345 564 353 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 965 1546 2425 2917 3215 965
Size: 581 879 492 298 2120
Fragments arranged by size:
2120 879 581 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4074 4074
Size: 4370
BsaAI yAC'GTr
Cuts at: 1723 1723
Size: 4370
BsaBI GATnn'nnATC
Cuts at: 6 283 1249 1884 3921 6
Size: 277 966 635 2037 455
Fragments arranged by size:
2037 966 635 455 277
BsaHI Gr'CG_yC
Cuts at: 1801 2049 2751 4247 1801
Size: 248 702 1496 1924
Fragments arranged by size:
1924 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1559 2080 2758 3032 3179 3590 1559
Size: 521 678 274 147 411 2339
Fragments arranged by size:
2339 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 121 151 121
Size: 30 4340
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1203 1203
Size: 4370
BseYI C'CCAG_C
Cuts at: 534 1173 1201 1795 2553 3130 3626 534
Size: 639 28 594 758 577 496 1278
Fragments arranged by size:
1278 758 639 594 577 496 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 365
Size: 4370
BsiEI CG_ry'CG
Cuts at: 104 1912 1958 3166 104
Size: 1808 46 1208 1308
Fragments arranged by size:
1808 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1384 2162 2352 3144 1384
Size: 778 190 792 2610
Fragments arranged by size:
2610 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1384 1995 2088 2162 2352 2414 3144 1384
Size: 611 93 74 190 62 730 2610
Fragments arranged by size:
2610 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3590 3590
Size: 4370
BspHI T'CATG_A
Cuts at: 648 3546 648
Size: 2898 1472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 197 1420 1634 1936 2317 197
Size: 1223 214 302 381 2250
Fragments arranged by size:
2250 1223 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2662 2716 4200 4365 50
Size: 2612 54 1484 165 55
Fragments arranged by size:
2612 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 869 2282 869
Size: 1413 2957
BsrFI r'CCGG_y
Cuts at: 2368 2549 2758 2368
Size: 181 209 3980
Fragments arranged by size:
3980 209 181
BsrGI T'GTAC_A
Cuts at: 1302 1302
Size: 4370
BssSI C'ACGA_G
Cuts at: 670 2641 2999 670
Size: 1971 358 2041
Fragments arranged by size:
2041 1971 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1747 1747
Size: 4370
BstBI TT'CG_AA
Cuts at: 2731 2731
Size: 4370
BstYI r'GATC_y
Cuts at: 1 420 1395 1862 1885 2220 2466 3478 3564
Size: 419 975 467 23 335 246 1012 86
Cuts at: 3564 3576 3922 1
Size: 12 346 449
Fragments arranged by size:
1012 975 467 449 419 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2320 2501 2664 2320
Size: 181 163 4026
Fragments arranged by size:
4026 181 163
BtsI GCAGTG_nn'
Cuts at: 1613 1613
Size: 4370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 479 514 479
Size: 35 4335
DraI TTT'AAA
Cuts at: 1369 1369
Size: 4370
DrdI GACnn_nn'nnGTC
Cuts at: 2076 2934 2076
Size: 858 3512
EaeI y'GGCC_r
Cuts at: 503 584 1955 2129 2520 2547 3892 503
Size: 81 1371 174 391 27 1345 981
Fragments arranged by size:
1371 1345 981 391 174 81 27
EagI C'GGCC_G
Cuts at: 1955 1955
Size: 4370
EarI CTCTTCn'nnn_
Cuts at: 689 1008 1034 2393 2603 689
Size: 319 26 1359 210 2456
Fragments arranged by size:
2456 1359 319 210 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2578 2888 3034 4180 2578
Size: 310 146 1146 2768
Fragments arranged by size:
2768 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1382 1382
Size: 4370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 231 713 795 955 989 1148 2195 2627
Size: 108 482 82 160 34 159 1047 432
Cuts at: 2627 3374 3642 123
Size: 747 268 851
Fragments arranged by size:
1047 851 747 482 432 268 160 159
108 82 34
EcoO109I rG'GnC_Cy
Cuts at: 1509 1509
Size: 4370
EcoRI G'AATT_C
Cuts at: 1374 1374
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 4370
FspI TGC'GCA
Cuts at: 2151 2151
Size: 4370
HaeII r_GCGC'y
Cuts at: 1804 2052 3074 4053 1804
Size: 248 1022 979 2121
Fragments arranged by size:
2121 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 121 153 327 359 688 720 804 836 2212
Size: 32 174 32 329 32 84 32 1376
Cuts at: 2212 2242 2244 2274 121
Size: 30 2 30 2217
Fragments arranged by size:
2217 1376 329 174 84 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1409 1409
Size: 4370
KasI G'GCGC_C
Cuts at: 1800 2048 1800
Size: 248 4122
KpnI G_GTAC'C
Cuts at: 189 1390 189
Size: 1201 3169
MluI A'CGCG_T
Cuts at: 3803 3803
Size: 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1016 1120 3041 3225 1016
Size: 104 1921 184 2161
Fragments arranged by size:
2161 1921 184 104
MscI TGG'CCA
Cuts at: 505 586 2131 505
Size: 81 1545 2744
Fragments arranged by size:
2744 1545 81
MslI CAynn'nnrTG
Cuts at: 151 469 653 856 2359 2486 151
Size: 318 184 203 1503 127 2035
Fragments arranged by size:
2035 1503 318 203 184 127
MspA1I CmG'CkG
Cuts at: 118 685 1126 1481 1795 2155 3168 3413 118
Size: 567 441 355 314 360 1013 245 1075
Fragments arranged by size:
1075 1013 567 441 360 355 314 245
NaeI GCC'GGC
Cuts at: 2551 2551
Size: 4370
NarI GG'CG_CC
Cuts at: 1801 2049 1801
Size: 248 4122
NgoMIV G'CCGG_C
Cuts at: 2549 2549
Size: 4370
NheI G'CTAG_C
Cuts at: 601 601
Size: 4370
NsiI A_TGCA'T
Cuts at: 472 472
Size: 4370
NspI r_CATG'y
Cuts at: 470 1423 1923 2830 470
Size: 953 500 907 2010
Fragments arranged by size:
2010 953 907 500
PasI CC'CwG_GG
Cuts at: 1325 1325
Size: 4370
PciI A'CATG_T
Cuts at: 2826 2826
Size: 4370
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 798 830 3509 3541 798
Size: 32 2679 32 1627
Fragments arranged by size:
2679 1627 32 32
PstI C_TGCA'G
Cuts at: 945 1417 2102 945
Size: 472 685 3213
Fragments arranged by size:
3213 685 472
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 118 685 1126 1795 2155 118
Size: 567 441 669 360 2333
Fragments arranged by size:
2333 669 567 441 360
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4370
SacI G_AGCT'C
Cuts at: 1384 1384
Size: 4370
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4370
SapI GCTCTTCn'nnn_
Cuts at: 2393 2603 2393
Size: 210 4160
SbfI CC_TGCA'GG
Cuts at: 1417 1417
Size: 4370
ScaI AGT'ACT
Cuts at: 3887 3887
Size: 4370
SfcI C'TryA_G
Cuts at: 33 941 1413 2098 3091 3282 33
Size: 908 472 685 993 191 1121
Fragments arranged by size:
1121 993 908 685 472 191
SfoI GGC'GCC
Cuts at: 1802 2050 1802
Size: 248 4122
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 1392 1392
Size: 4370
SmlI C'TyrA_G
Cuts at: 980 1525 2440 2932 3194 980
Size: 545 915 492 262 2156
Fragments arranged by size:
2156 915 545 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 1423 1923 1423
Size: 500 3870
StyI C'CwwG_G
Cuts at: 1504 1858 1504
Size: 354 4016
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1953 2086 2545 2754 1953
Size: 133 459 209 3569
Fragments arranged by size:
3569 459 209 133
TatI w'GTAC_w
Cuts at: 1302 3885 1302
Size: 2583 1787
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3887 3919 3887
Size: 32 4338
Tth111I GACn'n_nGTC
Cuts at: 1291 2167 1291
Size: 876 3494
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 594 1865 594
Size: 1271 3099
XmaI C'CCGG_G
Cuts at: 1390 1390
Size: 4370
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NheI NsiI NspI PasI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BmgBI Bpu10I BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NotI NruI PacI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI