Restriction map for
pF1KB7356
(Circular) (Six-base) MAPSORT of: pF1KB7356.seq Check: 2322 from: 1 to: 6644
pF1KB7356 6644 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2161 2161
Size: 6644
AccI GT'mk_AC
Cuts at: 3682 3682
Size: 6644
Acc65I G'GTAC_C
Cuts at: 3660 3660
Size: 6644
AclI AA'CG_TT
Cuts at: 6484 6484
Size: 6644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1321 1408 2349 2659 4469 4901 5648 5916
Size: 1173 87 941 310 1810 432 747 268
Cuts at: 5916 148
Size: 876
Fragments arranged by size:
1810 1173 941 876 747 432 310 268
87
AflIII A'CryG_T
Cuts at: 244 2350 3996 5100 6077 244
Size: 2106 1646 1104 977 811
Fragments arranged by size:
2106 1646 1104 977 811
AgeI A'CCGG_T
Cuts at: 5032 5032
Size: 6644
AleI CACnn'nnGTG
Cuts at: 2353 2353
Size: 6644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1522 1522
Size: 6644
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 848 880 848
Size: 32 6612
AlwNI CAG_nnn'CTG
Cuts at: 120 372 1299 1794 1811 3054 3949 5516 120
Size: 252 927 495 17 1243 895 1567 1248
Fragments arranged by size:
1567 1248 1243 927 895 495 252 17
ApaI G_GGCC'C
Cuts at: 1423 2988 1423
Size: 1565 5079
ApaLI G'TGCA_C
Cuts at: 3250 5414 3250
Size: 2164 4480
ApoI r'AATT_y
Cuts at: 478 894 3311 3648 5997 6253 478
Size: 416 2417 337 2349 256 869
Fragments arranged by size:
2417 2349 869 416 337 256
AseI AT'TA_AT
Cuts at: 20 2693 20
Size: 2673 3971
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6644
AvaI C'yCGr_G
Cuts at: 3664 3664
Size: 6644
BamHI G'GATC_C
Cuts at: 3669 3669
Size: 6644
BanI G'GyrC_C
Cuts at: 3660 4074 4322 4357 3660
Size: 414 248 35 5947
Fragments arranged by size:
5947 414 248 35
BanII G_rGCy'C
Cuts at: 1423 1697 2988 3035 3529 3580 3658 4688 1423
Size: 274 1291 47 494 51 78 1030 3379
Fragments arranged by size:
3379 1291 1030 494 274 78 51 47
BbeI G_GCGC'C
Cuts at: 4078 4326 4078
Size: 248 6396
BbsI GAAGACnn'nnnn_
Cuts at: 347 2981 347
Size: 2634 4010
BbvCI CC'TCA_GC
Cuts at: 1771 1771
Size: 6644
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6610
BciVI GTATCCnnnnn_n'
Cuts at: 2743 4542 5303 6644 2743
Size: 1799 761 1341 2743
Fragments arranged by size:
2743 1799 1341 761
BclI T'GATC_A
Cuts at: 2012 2418 4164 2012
Size: 406 1746 4492
Fragments arranged by size:
4492 1746 406
BfrBI ATG'CAT
Cuts at: 2223 2223
Size: 6644
BglII A'GATC_T
Cuts at: 4159 6196 4159
Size: 2037 4607
BlpI GC'TnA_GC
Cuts at: 3756 3756
Size: 6644
Bme1580I G_kGCm'C
Cuts at: 1423 1603 2988 3254 4269 4362 5418 1423
Size: 180 1385 266 1015 93 1056 2649
Fragments arranged by size:
2649 1385 1056 1015 266 180 93
BmrI ACTGGGnnnn_n'
Cuts at: 3856 3937 4272 6416 3856
Size: 81 335 2144 4084
Fragments arranged by size:
4084 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5011 5011
Size: 6644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 97 1267 1644 3482 97
Size: 1170 377 1838 3259
Fragments arranged by size:
3259 1838 1170 377
Bpu10I CC'TnA_GC
Cuts at: 1771 3409 1771
Size: 1638 5006
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3434 3820 4699 5191 5489 3434
Size: 386 879 492 298 4589
Fragments arranged by size:
4589 879 492 386 298
BsaI GGTCTCn'nnnn_
Cuts at: 6348 6348
Size: 6644
BsaAI yAC'GTr
Cuts at: 245 2351 3997 245
Size: 2106 1646 2892
Fragments arranged by size:
2892 2106 1646
BsaBI GATnn'nnATC
Cuts at: 6 4158 6195 6
Size: 4152 2037 455
Fragments arranged by size:
4152 2037 455
BsaHI Gr'CG_yC
Cuts at: 2400 2519 2546 2978 3493 4075 4323 5025 6521
Size: 119 27 432 515 582 248 702 1496
Cuts at: 6521 2400
Size: 2523
Fragments arranged by size:
2523 1496 702 582 515 432 248 119
27
BsaWI w'CCGG_w
Cuts at: 1331 3833 4354 5032 5306 5453 5864 1331
Size: 2502 521 678 274 147 411 2111
Fragments arranged by size:
2502 2111 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 304 334 304
Size: 30 6614
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 3487 325
Size: 3162 3482
BseYI C'CCAG_C
Cuts at: 1423 3530 4069 4827 5404 5900 1423
Size: 2107 539 758 577 496 2167
Fragments arranged by size:
2167 2107 758 577 539 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 477 1907 3569 348
Size: 129 1430 1662 3423
Fragments arranged by size:
3423 1662 1430 129
BsiEI CG_ry'CG
Cuts at: 104 4186 4232 5440 104
Size: 4082 46 1208 1308
Fragments arranged by size:
4082 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1331 3254 3580 3658 4436 4626 5418 1331
Size: 1923 326 78 778 190 792 2557
Fragments arranged by size:
2557 1923 792 778 326 190 78
BsmI GAATG_Cn'
Cuts at: 2787 2787
Size: 6644
Bsp1286I G_dGCh'C
Cuts at: 1331 1423 1603 1697 2988 3035 3254 3529 3580
Size: 92 180 94 1291 47 219 275 51
Cuts at: 3580 3658 4269 4362 4436 4626 4688 5418 1331
Size: 78 611 93 74 190 62 730 2557
Fragments arranged by size:
2557 1291 730 611 275 219 190 180
94 93 92 78 74 62 51 47
BspEI T'CCGG_A
Cuts at: 1331 5864 1331
Size: 4533 2111
BspHI T'CATG_A
Cuts at: 189 1991 5820 189
Size: 1802 3829 1013
Fragments arranged by size:
3829 1802 1013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 558 2161 3132 3694 3908 4210 4591 558
Size: 1603 971 562 214 302 381 2611
Fragments arranged by size:
2611 1603 971 562 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2542 4936 4990 6474 6639 50
Size: 2492 2394 54 1484 165 55
Fragments arranged by size:
2492 2394 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1041 2052 4556 1041
Size: 1011 2504 3129
Fragments arranged by size:
3129 2504 1011
BsrFI r'CCGG_y
Cuts at: 4642 4823 5032 4642
Size: 181 209 6254
Fragments arranged by size:
6254 209 181
BssSI C'ACGA_G
Cuts at: 4915 5273 4915
Size: 358 6286
BstAPI GCAn_nnn'nTGC
Cuts at: 3508 4021 3508
Size: 513 6131
BstBI TT'CG_AA
Cuts at: 5005 5005
Size: 6644
BstEII G'GTnAC_C
Cuts at: 943 943
Size: 6644
BstXI CCAn_nnnn'nTGG
Cuts at: 3327 3595 3327
Size: 268 6376
BstYI r'GATC_y
Cuts at: 1 138 2669 2823 3669 4136 4159 4494 4740
Size: 137 2531 154 846 467 23 335 246
Cuts at: 4740 5752 5838 5850 6196 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2531 1012 846 467 449 346 335 246
154 137 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 949 1804 3536 949
Size: 855 1732 4057
Fragments arranged by size:
4057 1732 855
BtgI C'CryG_G
Cuts at: 1319 2021 3320 1319
Size: 702 1299 4643
Fragments arranged by size:
4643 1299 702
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4594 4775 4938 4594
Size: 181 163 6300
Fragments arranged by size:
6300 181 163
BtsI GCAGTG_nn'
Cuts at: 3887 3887
Size: 6644
ClaI AT'CG_AT
Cuts at: 3014 3014
Size: 6644
DraI TTT'AAA
Cuts at: 1055 1244 1890 3275 3643 1055
Size: 189 646 1385 368 4056
Fragments arranged by size:
4056 1385 646 368 189
DraIII CAC_nnn'GTG
Cuts at: 889 889
Size: 6644
DrdI GACnn_nn'nnGTC
Cuts at: 2320 2551 4350 5208 2320
Size: 231 1799 858 3756
Fragments arranged by size:
3756 1799 858 231
EaeI y'GGCC_r
Cuts at: 838 1093 2333 2799 4229 4403 4794 4821 6166
Size: 255 1240 466 1430 174 391 27 1345
Cuts at: 6166 838
Size: 1316
Fragments arranged by size:
1430 1345 1316 1240 466 391 255 174
27
EagI C'GGCC_G
Cuts at: 4229 4229
Size: 6644
EarI CTCTTCn'nnn_
Cuts at: 1403 1673 2021 4667 4877 1403
Size: 270 348 2646 210 3170
Fragments arranged by size:
3170 2646 348 270 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4852 5162 5308 6454 4852
Size: 310 146 1146 5042
Fragments arranged by size:
5042 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3578 3656 3578
Size: 78 6566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 97 148 1267 1321 1408 1644 2349 2659 3482
Size: 51 1119 54 87 236 705 310 823
Cuts at: 3482 4469 4901 5648 5916 97
Size: 987 432 747 268 825
Fragments arranged by size:
1119 987 825 823 747 705 432 310
268 236 87 54 51
EcoNI CCTnn'n_nnAGG
Cuts at: 1390 1828 1390
Size: 438 6206
EcoO109I rG'GnC_Cy
Cuts at: 1305 1420 2553 2985 3783 1305
Size: 115 1133 432 798 4166
Fragments arranged by size:
4166 1133 798 432 115
EcoRI G'AATT_C
Cuts at: 3648 3648
Size: 6644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1368 1520 4663 1368
Size: 152 3143 3349
Fragments arranged by size:
3349 3143 152
FspI TGC'GCA
Cuts at: 4425 4425
Size: 6644
HaeII r_GCGC'y
Cuts at: 4078 4326 5348 6327 4078
Size: 248 1022 979 4395
Fragments arranged by size:
4395 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 123 155 1258 1290 3451 3483 4486 4516 4518
Size: 32 1103 32 2161 32 1003 30 2
Cuts at: 4518 4548 123
Size: 30 2219
Fragments arranged by size:
2219 2161 1103 1003 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1529 3683 1529
Size: 2154 4490
HindIII A'AGCT_T
Cuts at: 1723 1723
Size: 6644
KasI G'GCGC_C
Cuts at: 4074 4322 4074
Size: 248 6396
KpnI G_GTAC'C
Cuts at: 3664 3664
Size: 6644
MfeI C'AATT_G
Cuts at: 1688 1688
Size: 6644
MluI A'CGCG_T
Cuts at: 6077 6077
Size: 6644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1926 2076 2936 3165 5315 5499 1926
Size: 150 860 229 2150 184 3071
Fragments arranged by size:
3071 2150 860 229 184 150
MscI TGG'CCA
Cuts at: 840 1095 2335 4405 840
Size: 255 1240 2070 3079
Fragments arranged by size:
3079 2070 1240 255
MslI CAynn'nnrTG
Cuts at: 2020 2353 3325 4633 4760 2020
Size: 333 972 1308 127 3904
Fragments arranged by size:
3904 1308 972 333 127
MspA1I CmG'CkG
Cuts at: 1374 3051 3755 4069 4429 5442 5687 1374
Size: 1677 704 314 360 1013 245 2331
Fragments arranged by size:
2331 1677 1013 704 360 314 245
NaeI GCC'GGC
Cuts at: 4825 4825
Size: 6644
NarI GG'CG_CC
Cuts at: 4075 4323 4075
Size: 248 6396
NcoI C'CATG_G
Cuts at: 2021 3320 2021
Size: 1299 5345
NgoMIV G'CCGG_C
Cuts at: 4823 4823
Size: 6644
NsiI A_TGCA'T
Cuts at: 2225 2225
Size: 6644
NspI r_CATG'y
Cuts at: 1656 2415 3697 4197 5104 1656
Size: 759 1282 500 907 3196
Fragments arranged by size:
3196 1282 907 759 500
PasI CC'CwG_GG
Cuts at: 1603 1603
Size: 6644
PciI A'CATG_T
Cuts at: 5100 5100
Size: 6644
PflMI CCAn_nnn'nTGG
Cuts at: 120 372 560 2514 120
Size: 252 188 1954 4250
Fragments arranged by size:
4250 1954 252 188
PfoI T'CCnGG_A
Cuts at: 1823 1823
Size: 6644
PmeI GTTT'AAAC
Cuts at: 3643 3643
Size: 6644
PmlI CAC'GTG
Cuts at: 245 2351 245
Size: 2106 4538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5783 5815 5783
Size: 32 6612
PpuMI rG'GwC_Cy
Cuts at: 2553 2553
Size: 6644
PsiI TTA'TAA
Cuts at: 163 3296 163
Size: 3133 3511
PspOMI G'GGCC_C
Cuts at: 1419 2984 1419
Size: 1565 5079
PstI C_TGCA'G
Cuts at: 1238 1313 2451 3524 3691 4376 1238
Size: 75 1138 1073 167 685 3506
Fragments arranged by size:
3506 1138 1073 685 167 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6644
PvuII CAG'CTG
Cuts at: 3051 4069 4429 3051
Size: 1018 360 5266
Fragments arranged by size:
5266 1018 360
RsrII CG'GwC_CG
Cuts at: 4839 4839
Size: 6644
SacI G_AGCT'C
Cuts at: 3580 3658 3580
Size: 78 6566
SalI G'TCGA_C
Cuts at: 3681 3681
Size: 6644
SapI GCTCTTCn'nnn_
Cuts at: 1403 4667 4877 1403
Size: 3264 210 3170
Fragments arranged by size:
3264 3170 210
SbfI CC_TGCA'GG
Cuts at: 3691 3691
Size: 6644
ScaI AGT'ACT
Cuts at: 537 583 6161 537
Size: 46 5578 1020
Fragments arranged by size:
5578 1020 46
SfcI C'TryA_G
Cuts at: 33 465 1234 1309 2159 2447 3111 3520 3687
Size: 432 769 75 850 288 664 409 167
Cuts at: 3687 4372 5365 5556 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 850 769 685 664 432 409
288 191 167 75
SfoI GGC'GCC
Cuts at: 4076 4324 4076
Size: 248 6396
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6644
SmaI CCC'GGG
Cuts at: 3666 3666
Size: 6644
SmlI C'TyrA_G
Cuts at: 3449 3799 4714 5206 5468 3449
Size: 350 915 492 262 4625
Fragments arranged by size:
4625 915 492 350 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6644
SphI G_CATG'C
Cuts at: 3697 4197 3697
Size: 500 6144
SspI AAT'ATT
Cuts at: 1899 2207 1899
Size: 308 6336
StuI AGG'CCT
Cuts at: 1665 2622 1665
Size: 957 5687
StyI C'CwwG_G
Cuts at: 1729 2021 2549 3320 3778 4132 1729
Size: 292 528 771 458 354 4241
Fragments arranged by size:
4241 771 528 458 354 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1595 2696 4227 4360 4819 5028 1595
Size: 1101 1531 133 459 209 3211
Fragments arranged by size:
3211 1531 1101 459 209 133
TatI w'GTAC_w
Cuts at: 488 535 581 1088 2061 6159 488
Size: 47 46 507 973 4098 973
Fragments arranged by size:
4098 973 973 507 47 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 304 336 6161 6193 304
Size: 32 5825 32 755
Fragments arranged by size:
5825 755 32 32
Tth111I GACn'n_nGTC
Cuts at: 2320 4441 2320
Size: 2121 4523
XbaI T'CTAG_A
Cuts at: 3675 3675
Size: 6644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 149 3595 4139 149
Size: 3446 544 2654
Fragments arranged by size:
3446 2654 544
XmaI C'CCGG_G
Cuts at: 3664 3664
Size: 6644
XmnI GAAnn'nnTTC
Cuts at: 229 229
Size: 6644
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BaeI BglI
BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I CspCI
EcoRV FseI FspAI HpaI NdeI NheI NotI NruI
PacI PshAI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI