Restriction map for
pF1KB7351
(Circular) (Six-base) MAPSORT of: pF1KB7351.seq Check: 1186 from: 1 to: 6881
pF1KB7351 6881 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 693 693
Size: 6881
AatII G_ACGT'C
Cuts at: 3458 3458
Size: 6881
AccI GT'mk_AC
Cuts at: 1150 2976 3862 3919 1150
Size: 1826 886 57 4112
Fragments arranged by size:
4112 1826 886 57
Acc65I G'GTAC_C
Cuts at: 2874 3897 2874
Size: 1023 5858
AclI AA'CG_TT
Cuts at: 3783 6721 3783
Size: 2938 3943
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 821 1100 1939 1978 2377 2645 2674 2755 2792
Size: 279 839 39 399 268 29 81 37
Cuts at: 2792 3752 4706 5138 5885 6153 821
Size: 960 954 432 747 268 1549
Fragments arranged by size:
1549 960 954 839 747 432 399 279
268 268 81 39 37 29
AflIII A'CryG_T
Cuts at: 4233 5337 6314 4233
Size: 1104 977 4800
Fragments arranged by size:
4800 1104 977
AgeI A'CCGG_T
Cuts at: 5269 5269
Size: 6881
AleI CACnn'nnGTG
Cuts at: 2699 2699
Size: 6881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3314 3314
Size: 6881
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2097 2129 2097
Size: 32 6849
AlwNI CAG_nnn'CTG
Cuts at: 1459 3858 4186 5753 1459
Size: 2399 328 1567 2587
Fragments arranged by size:
2587 2399 1567 328
ApaLI G'TGCA_C
Cuts at: 5651 5651
Size: 6881
ApoI r'AATT_y
Cuts at: 235 546 801 1238 1944 2271 2421 2534 3014
Size: 311 255 437 706 327 150 113 480
Cuts at: 3014 3190 3640 3885 6234 6490 235
Size: 176 450 245 2349 256 626
Fragments arranged by size:
2349 706 626 480 450 437 327 311
256 255 245 176 150 113
AseI AT'TA_AT
Cuts at: 20 3659 20
Size: 3639 3242
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6881
AvaI C'yCGr_G
Cuts at: 2357 3901 2357
Size: 1544 5337
BamHI G'GATC_C
Cuts at: 3204 3906 3204
Size: 702 6179
BanI G'GyrC_C
Cuts at: 1512 2874 3298 3897 4311 4559 4594 1512
Size: 1362 424 599 414 248 35 3799
Fragments arranged by size:
3799 1362 599 424 414 248 35
BanII G_rGCy'C
Cuts at: 195 725 2347 3749 3895 4925 195
Size: 530 1622 1402 146 1030 2151
Fragments arranged by size:
2151 1622 1402 1030 530 146
BbeI G_GCGC'C
Cuts at: 4315 4563 4315
Size: 248 6633
BbsI GAAGACnn'nnnn_
Cuts at: 275 455 1140 3099 3636 275
Size: 180 685 1959 537 3520
Fragments arranged by size:
3520 1959 685 537 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2008 2042 90
Size: 34 1884 34 4929
Fragments arranged by size:
4929 1884 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4779 5540 6881 4779
Size: 761 1341 4779
Fragments arranged by size:
4779 1341 761
BclI T'GATC_A
Cuts at: 1932 4401 1932
Size: 2469 4412
BfrBI ATG'CAT
Cuts at: 1765 1765
Size: 6881
BglI GCCn_nnn'nGGC
Cuts at: 262 262
Size: 6881
BglII A'GATC_T
Cuts at: 4396 6433 4396
Size: 2037 4844
BlpI GC'TnA_GC
Cuts at: 1403 3993 1403
Size: 2590 4291
Bme1580I G_kGCm'C
Cuts at: 2532 2862 4506 4599 5655 2532
Size: 330 1644 93 1056 3758
Fragments arranged by size:
3758 1644 1056 330 93
BmrI ACTGGGnnnn_n'
Cuts at: 1796 4093 4174 4509 6653 1796
Size: 2297 81 335 2144 2024
Fragments arranged by size:
2297 2144 2024 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5248 5248
Size: 6881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 106 1250 1481 106
Size: 1144 231 5506
Fragments arranged by size:
5506 1144 231
Bpu10I CC'TnA_GC
Cuts at: 368 620 2322 368
Size: 252 1702 4927
Fragments arranged by size:
4927 1702 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4057 4936 5428 5726 4057
Size: 879 492 298 5212
Fragments arranged by size:
5212 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3565 6585 3565
Size: 3020 3861
BsaAI yAC'GTr
Cuts at: 4234 4234
Size: 6881
BsaBI GATnn'nnATC
Cuts at: 6 355 4395 6432 6
Size: 349 4040 2037 455
Fragments arranged by size:
4040 2037 455 349
BsaHI Gr'CG_yC
Cuts at: 2973 3455 4312 4560 5262 6758 2973
Size: 482 857 248 702 1496 3096
Fragments arranged by size:
3096 1496 857 702 482 248
BsaWI w'CCGG_w
Cuts at: 4070 4591 5269 5543 5690 6101 4070
Size: 521 678 274 147 411 4850
Fragments arranged by size:
4850 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 892 2132 2596 2599 2923 892
Size: 1240 464 3 324 4850
Fragments arranged by size:
4850 1240 464 324 3
BseYI C'CCAG_C
Cuts at: 4306 5064 5641 6137 4306
Size: 758 577 496 5050
Fragments arranged by size:
5050 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2921 3470 3851 2921
Size: 549 381 5951
Fragments arranged by size:
5951 549 381
BsiEI CG_ry'CG
Cuts at: 104 4423 4469 5677 104
Size: 4319 46 1208 1308
Fragments arranged by size:
4319 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 170 1266 1467 1863 2142 2347 3895 4673 4863
Size: 1096 201 396 279 205 1548 778 190
Cuts at: 4863 5655 170
Size: 792 1396
Fragments arranged by size:
1548 1396 1096 792 778 396 279 205
201 190
BsmI GAATG_Cn'
Cuts at: 1001 3356 3629 1001
Size: 2355 273 4253
Fragments arranged by size:
4253 2355 273
BsmBI CGTCTCn'nnnn_
Cuts at: 1416 1416
Size: 6881
Bsp1286I G_dGCh'C
Cuts at: 170 195 725 1266 1467 1863 2142 2347 2532
Size: 25 530 541 201 396 279 205 185
Cuts at: 2532 2862 3749 3895 4506 4599 4673 4863 4925
Size: 330 887 146 611 93 74 190 62
Cuts at: 4925 5655 170
Size: 730 1396
Fragments arranged by size:
1396 887 730 611 541 530 396 330
279 205 201 190 185 146 93 74
62 25
BspEI T'CCGG_A
Cuts at: 6101 6101
Size: 6881
BspHI T'CATG_A
Cuts at: 2838 3138 3701 6057 2838
Size: 300 563 2356 3662
Fragments arranged by size:
3662 2356 563 300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 693 2690 2886 3931 4145 4447 4828 693
Size: 1997 196 1045 214 302 381 2746
Fragments arranged by size:
2746 1997 1045 381 302 214 196
BsrBI CCG'CTC
Cuts at: 50 120 197 5173 5227 6711 6876 50
Size: 70 77 4976 54 1484 165 55
Fragments arranged by size:
4976 1484 165 77 70 55 54
BsrDI GCAATG_nn'
Cuts at: 4793 4793
Size: 6881
BsrFI r'CCGG_y
Cuts at: 4879 5060 5269 4879
Size: 181 209 6491
Fragments arranged by size:
6491 209 181
BssSI C'ACGA_G
Cuts at: 3176 5152 5510 3176
Size: 1976 358 4547
Fragments arranged by size:
4547 1976 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3249 3351 4258 3249
Size: 102 907 5872
Fragments arranged by size:
5872 907 102
BstBI TT'CG_AA
Cuts at: 5242 5242
Size: 6881
BstYI r'GATC_y
Cuts at: 1 570 1074 3204 3906 4373 4396 4731 4977
Size: 569 504 2130 702 467 23 335 246
Cuts at: 4977 5989 6075 6087 6433 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2130 1012 702 569 504 467 449 346
335 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1151 1151
Size: 6881
Bsu36I CC'TnA_GG
Cuts at: 411 3104 411
Size: 2693 4188
BtgI C'CryG_G
Cuts at: 3044 3044
Size: 6881
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4831 5012 5175 4831
Size: 181 163 6537
Fragments arranged by size:
6537 181 163
BtsI GCAGTG_nn'
Cuts at: 1659 4124 1659
Size: 2465 4416
DraI TTT'AAA
Cuts at: 3880 3880
Size: 6881
DraIII CAC_nnn'GTG
Cuts at: 174 3874 174
Size: 3700 3181
DrdI GACnn_nn'nnGTC
Cuts at: 4587 5445 4587
Size: 858 6023
EaeI y'GGCC_r
Cuts at: 128 263 4466 4640 5031 5058 6403 128
Size: 135 4203 174 391 27 1345 606
Fragments arranged by size:
4203 1345 606 391 174 135 27
EagI C'GGCC_G
Cuts at: 4466 4466
Size: 6881
EarI CTCTTCn'nnn_
Cuts at: 147 239 599 1523 2546 2600 2774 2900 2960
Size: 92 360 924 1023 54 174 126 60
Cuts at: 2960 3262 4904 5114 147
Size: 302 1642 210 1914
Fragments arranged by size:
1914 1642 1023 924 360 302 210 174
126 92 60 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3745 5089 5399 5545 6691 3745
Size: 1344 310 146 1146 3935
Fragments arranged by size:
3935 1344 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2345 3893 2345
Size: 1548 5333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 106 821 1100 1250 1481 1939 1978 2377 2645
Size: 715 279 150 231 458 39 399 268
Cuts at: 2645 2674 2755 2792 3752 4706 5138 5885 6153
Size: 29 81 37 960 954 432 747 268
Cuts at: 6153 106
Size: 834
Fragments arranged by size:
960 954 834 747 715 458 432 399
279 268 268 231 150 81 39 37
29
EcoNI CCTnn'n_nnAGG
Cuts at: 1316 1316
Size: 6881
EcoO109I rG'GnC_Cy
Cuts at: 811 3855 4020 811
Size: 3044 165 3672
Fragments arranged by size:
3672 3044 165
EcoRI G'AATT_C
Cuts at: 2534 3885 2534
Size: 1351 5530
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2822 4900 2822
Size: 2078 4803
FspI TGC'GCA
Cuts at: 1651 4662 1651
Size: 3011 3870
HaeII r_GCGC'y
Cuts at: 1670 4315 4563 5585 6564 1670
Size: 2645 248 1022 979 1987
Fragments arranged by size:
2645 1987 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 598 630 1686 1718 3547 3579 3709 3741 4723
Size: 32 1056 32 1829 32 130 32 982
Cuts at: 4723 4753 4755 4785 598
Size: 30 2 30 2694
Fragments arranged by size:
2694 1829 1056 982 130 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 394 2814 3309 3687 3863 3920 394
Size: 2420 495 378 176 57 3355
Fragments arranged by size:
3355 2420 495 378 176 57
HindIII A'AGCT_T
Cuts at: 331 331
Size: 6881
KasI G'GCGC_C
Cuts at: 4311 4559 4311
Size: 248 6633
KpnI G_GTAC'C
Cuts at: 2878 3901 2878
Size: 1023 5858
MluI A'CGCG_T
Cuts at: 6314 6314
Size: 6881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 263 989 1370 1884 2407 5552 5736 263
Size: 726 381 514 523 3145 184 1408
Fragments arranged by size:
3145 1408 726 523 514 381 184
MscI TGG'CCA
Cuts at: 4642 4642
Size: 6881
MslI CAynn'nnrTG
Cuts at: 307 2699 4870 4997 307
Size: 2392 2171 127 2191
Fragments arranged by size:
2392 2191 2171 127
MspA1I CmG'CkG
Cuts at: 133 3801 3992 4306 4666 5679 5924 133
Size: 3668 191 314 360 1013 245 1090
Fragments arranged by size:
3668 1090 1013 360 314 245 191
NaeI GCC'GGC
Cuts at: 5062 5062
Size: 6881
NarI GG'CG_CC
Cuts at: 4312 4560 4312
Size: 248 6633
NcoI C'CATG_G
Cuts at: 3044 3044
Size: 6881
NdeI CA'TA_TG
Cuts at: 846 2235 2330 846
Size: 1389 95 5397
Fragments arranged by size:
5397 1389 95
NgoMIV G'CCGG_C
Cuts at: 5060 5060
Size: 6881
NsiI A_TGCA'T
Cuts at: 1767 1767
Size: 6881
NspI r_CATG'y
Cuts at: 3934 4434 5341 3934
Size: 500 907 5474
Fragments arranged by size:
5474 907 500
PasI CC'CwG_GG
Cuts at: 2117 2117
Size: 6881
PciI A'CATG_T
Cuts at: 5337 5337
Size: 6881
PfoI T'CCnGG_A
Cuts at: 1783 3825 1783
Size: 2042 4839
PmeI GTTT'AAAC
Cuts at: 3880 3880
Size: 6881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6020 6052 6020
Size: 32 6849
PpuMI rG'GwC_Cy
Cuts at: 811 811
Size: 6881
PstI C_TGCA'G
Cuts at: 1848 3928 4613 1848
Size: 2080 685 4116
Fragments arranged by size:
4116 2080 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6881
PvuII CAG'CTG
Cuts at: 3801 4306 4666 3801
Size: 505 360 6016
Fragments arranged by size:
6016 505 360
RsrII CG'GwC_CG
Cuts at: 147 5076 147
Size: 4929 1952
SacI G_AGCT'C
Cuts at: 2347 3895 2347
Size: 1548 5333
SalI G'TCGA_C
Cuts at: 3861 3918 3861
Size: 57 6824
SapI GCTCTTCn'nnn_
Cuts at: 239 2600 2774 4904 5114 239
Size: 2361 174 2130 210 2006
Fragments arranged by size:
2361 2130 2006 210 174
SbfI CC_TGCA'GG
Cuts at: 3928 3928
Size: 6881
ScaI AGT'ACT
Cuts at: 717 6398 717
Size: 5681 1200
SfcI C'TryA_G
Cuts at: 33 1305 1844 2756 3734 3924 4609 5602 5793
Size: 1272 539 912 978 190 685 993 191
Cuts at: 5793 33
Size: 1121
Fragments arranged by size:
1272 1121 993 978 912 685 539 191
190
SfoI GGC'GCC
Cuts at: 4313 4561 4313
Size: 248 6633
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6881
SmaI CCC'GGG
Cuts at: 3903 3903
Size: 6881
SmlI C'TyrA_G
Cuts at: 4036 4951 5443 5705 4036
Size: 915 492 262 5212
Fragments arranged by size:
5212 915 492 262
SpeI A'CTAG_T
Cuts at: 67 3367 67
Size: 3300 3581
SphI G_CATG'C
Cuts at: 3934 4434 3934
Size: 500 6381
StyI C'CwwG_G
Cuts at: 1671 3044 4015 4369 1671
Size: 1373 971 354 4183
Fragments arranged by size:
4183 1373 971 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 135 1620 1885 4464 4597 5056 5265 135
Size: 1485 265 2579 133 459 209 1751
Fragments arranged by size:
2579 1751 1485 459 265 209 133
TatI w'GTAC_w
Cuts at: 715 6396 715
Size: 5681 1200
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6398 6430 6398
Size: 32 6849
Tth111I GACn'n_nGTC
Cuts at: 4678 4678
Size: 6881
XbaI T'CTAG_A
Cuts at: 162 2262 3912 162
Size: 2100 1650 3131
Fragments arranged by size:
3131 2100 1650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4376 4376
Size: 6881
XmaI C'CCGG_G
Cuts at: 3901 3901
Size: 6881
XmnI GAAnn'nnTTC
Cuts at: 854 1137 1352 1792 3784 854
Size: 283 215 440 1992 3951
Fragments arranged by size:
3951 1992 440 283 215
ZraI GAC'GTC
Cuts at: 3456 3456
Size: 6881
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI ApaI AscI AvrII BaeI BbvCI
BmgBI BmtI BsaXI BsiWI BsrGI BssHII BstEII BstXI
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI PacI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI