Restriction map for
pF1KB7349
(Circular) (Six-base) MAPSORT of: pF1KB7349.seq Check: 1645 from: 1 to: 6161
pF1KB7349 6161 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 420 947 2679 2691 420
Size: 527 1732 12 3890
Fragments arranged by size:
3890 1732 527 12
AatII G_ACGT'C
Cuts at: 128 128
Size: 6161
AccI GT'mk_AC
Cuts at: 3199 3199
Size: 6161
Acc65I G'GTAC_C
Cuts at: 3177 3177
Size: 6161
AclI AA'CG_TT
Cuts at: 6001 6001
Size: 6161
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 664 751 763 1704 1791 1844 1999 2377
Size: 99 87 12 941 87 53 155 378
Cuts at: 2377 2392 2509 2782 3986 4418 5165 5433 565
Size: 15 117 273 1204 432 747 268 1293
Fragments arranged by size:
1293 1204 941 747 432 378 273 268
155 117 99 87 87 53 15 12
AfeI AGC'GCT
Cuts at: 1201 2158 2592 1201
Size: 957 434 4770
Fragments arranged by size:
4770 957 434
AflIII A'CryG_T
Cuts at: 143 3513 4617 5594 143
Size: 3370 1104 977 710
Fragments arranged by size:
3370 1104 977 710
AgeI A'CCGG_T
Cuts at: 4549 4549
Size: 6161
AleI CACnn'nnGTG
Cuts at: 3068 3068
Size: 6161
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2194 2435 2759 2980 2194
Size: 241 324 221 5375
Fragments arranged by size:
5375 324 241 221
AlwNI CAG_nnn'CTG
Cuts at: 162 174 435 1563 2031 3108 3466 5033 162
Size: 12 261 1128 468 1077 358 1567 1290
Fragments arranged by size:
1567 1290 1128 1077 468 358 261 12
ApaI G_GGCC'C
Cuts at: 1504 2525 1504
Size: 1021 5140
ApaLI G'TGCA_C
Cuts at: 736 4931 736
Size: 4195 1966
ApoI r'AATT_y
Cuts at: 2059 3165 5514 5770 2059
Size: 1106 2349 256 2450
Fragments arranged by size:
2450 2349 1106 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6161
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6161
AvaI C'yCGr_G
Cuts at: 343 991 1597 2203 2951 3181 343
Size: 648 606 606 748 230 3323
Fragments arranged by size:
3323 748 648 606 606 230
BamHI G'GATC_C
Cuts at: 1748 3009 3186 1748
Size: 1261 177 4723
Fragments arranged by size:
4723 1261 177
BanI G'GyrC_C
Cuts at: 3070 3177 3591 3839 3874 3070
Size: 107 414 248 35 5357
Fragments arranged by size:
5357 414 248 107 35
BanII G_rGCy'C
Cuts at: 344 992 1142 1504 1654 1664 1745 2462 2525
Size: 648 150 362 150 10 81 717 63
Cuts at: 2525 2998 3044 3175 4205 344
Size: 473 46 131 1030 2300
Fragments arranged by size:
2300 1030 717 648 473 362 150 150
131 81 63 46 10
BbeI G_GCGC'C
Cuts at: 3595 3843 3595
Size: 248 5913
BbsI GAAGACnn'nnnn_
Cuts at: 520 1832 2497 520
Size: 1312 665 4184
Fragments arranged by size:
4184 1312 665
BbvCI CC'TCA_GC
Cuts at: 1877 1877
Size: 6161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1294 1328 90
Size: 34 1170 34 4923
Fragments arranged by size:
4923 1170 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4059 4820 6161 4059
Size: 761 1341 4059
Fragments arranged by size:
4059 1341 761
BclI T'GATC_A
Cuts at: 3681 3681
Size: 6161
BglI GCCn_nnn'nGGC
Cuts at: 1339 1898 1339
Size: 559 5602
BglII A'GATC_T
Cuts at: 3676 5713 3676
Size: 2037 4124
BlpI GC'TnA_GC
Cuts at: 425 772 1087 1141 2261 3273 425
Size: 347 315 54 1120 1012 3313
Fragments arranged by size:
3313 1120 1012 347 315 54
Bme1580I G_kGCm'C
Cuts at: 740 1504 2525 3075 3786 3879 4935 740
Size: 764 1021 550 711 93 1056 1966
Fragments arranged by size:
1966 1056 1021 764 711 550 93
BmgBI CAC'GTC
Cuts at: 144 1056 144
Size: 912 5249
BmrI ACTGGGnnnn_n'
Cuts at: 1266 3373 3454 3789 5933 1266
Size: 2107 81 335 2144 1494
Fragments arranged by size:
2144 2107 1494 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1647 1932 4528 1647
Size: 285 2596 3280
Fragments arranged by size:
3280 2596 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 310 394 469 814 1180 1261 2023 2071
Size: 177 84 75 345 366 81 762 48
Cuts at: 2071 2085 2658 2797 133
Size: 14 573 139 3497
Fragments arranged by size:
3497 762 573 366 345 177 139 84
81 75 48 14
Bpu10I CC'TnA_GC
Cuts at: 1543 1877 2140 2725 1543
Size: 334 263 585 4979
Fragments arranged by size:
4979 585 334 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2137 2446 3337 4216 4708 5006 2137
Size: 309 891 879 492 298 3292
Fragments arranged by size:
3292 891 879 492 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 312 715 5865 312
Size: 403 5150 608
Fragments arranged by size:
5150 608 403
BsaAI yAC'GTr
Cuts at: 3514 3514
Size: 6161
BsaBI GATnn'nnATC
Cuts at: 6 3675 5712 6
Size: 3669 2037 455
Fragments arranged by size:
3669 2037 455
BsaHI Gr'CG_yC
Cuts at: 125 1250 3592 3840 4542 6038 125
Size: 1125 2342 248 702 1496 248
Fragments arranged by size:
2342 1496 1125 702 248 248
BsaWI w'CCGG_w
Cuts at: 3350 3871 4549 4823 4970 5381 3350
Size: 521 678 274 147 411 4130
Fragments arranged by size:
4130 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 130 160 1198 1228 130
Size: 30 1038 30 5063
Fragments arranged by size:
5063 1038 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 391 394 586 649 706 769 969 970
Size: 240 3 192 63 57 63 200 1
Cuts at: 970 1120 1174 1957 2263 2572 3109 151
Size: 150 54 783 306 309 537 3203
Fragments arranged by size:
3203 783 537 309 306 240 200 192
150 63 63 57 54 3 1
BseYI C'CCAG_C
Cuts at: 891 1795 2099 2182 3137 3586 4344 4921 5417
Size: 904 304 83 955 449 758 577 496
Cuts at: 5417 891
Size: 1635
Fragments arranged by size:
1635 955 904 758 577 496 449 304
83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 557 2110 2268 544
Size: 13 1553 158 4437
Fragments arranged by size:
4437 1553 158 13
BsiEI CG_ry'CG
Cuts at: 104 457 1118 3703 3749 4957 104
Size: 353 661 2585 46 1208 1308
Fragments arranged by size:
2585 1308 1208 661 353 46
BsiHKAI G_wGCw'C
Cuts at: 344 740 992 1046 1088 1142 1184 1606 2462
Size: 396 252 54 42 54 42 422 856
Cuts at: 2462 2998 3175 3953 4143 4935 344
Size: 536 177 778 190 792 1570
Fragments arranged by size:
1570 856 792 778 536 422 396 252
190 177 54 54 42 42
BsmI GAATG_Cn'
Cuts at: 465 1572 2265 465
Size: 1107 693 4361
Fragments arranged by size:
4361 1107 693
BsmBI CGTCTCn'nnnn_
Cuts at: 1237 1237
Size: 6161
Bsp1286I G_dGCh'C
Cuts at: 344 740 992 1046 1088 1142 1184 1504 1606
Size: 396 252 54 42 54 42 320 102
Cuts at: 1606 1654 1664 1745 2462 2525 2998 3044 3075
Size: 48 10 81 717 63 473 46 31
Cuts at: 3075 3175 3786 3879 3953 4143 4205 4935 344
Size: 100 611 93 74 190 62 730 1570
Fragments arranged by size:
1570 730 717 611 473 396 320 252
190 102 100 93 81 74 63 62
54 54 48 46 42 42 31 10
BspEI T'CCGG_A
Cuts at: 5381 5381
Size: 6161
BspHI T'CATG_A
Cuts at: 2132 5337 2132
Size: 3205 2956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 202 420 776 947 1736 2679 2691 3211 3425
Size: 218 356 171 789 943 12 520 214
Cuts at: 3425 3727 4108 202
Size: 302 381 2255
Fragments arranged by size:
2255 943 789 520 381 356 302 218
214 171 12
BsrBI CCG'CTC
Cuts at: 50 491 653 1037 1926 2451 2514 2667 4453
Size: 441 162 384 889 525 63 153 1786
Cuts at: 4453 4507 5991 6156 50
Size: 54 1484 165 55
Fragments arranged by size:
1786 1484 889 525 441 384 165 162
153 63 55 54
BsrDI GCAATG_nn'
Cuts at: 4073 4073
Size: 6161
BsrFI r'CCGG_y
Cuts at: 212 256 4159 4340 4549 212
Size: 44 3903 181 209 1824
Fragments arranged by size:
3903 1824 209 181 44
BssHII G'CGCG_C
Cuts at: 677 677
Size: 6161
BssSI C'ACGA_G
Cuts at: 1045 1303 4432 4790 1045
Size: 258 3129 358 2416
Fragments arranged by size:
3129 2416 358 258
BstAPI GCAn_nnn'nTGC
Cuts at: 162 194 435 1869 3538 162
Size: 32 241 1434 1669 2785
Fragments arranged by size:
2785 1669 1434 241 32
BstBI TT'CG_AA
Cuts at: 4522 4522
Size: 6161
BstYI r'GATC_y
Cuts at: 1 1748 2819 3009 3186 3653 3676 4011 4257
Size: 1747 1071 190 177 467 23 335 246
Cuts at: 4257 5269 5355 5367 5713 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1747 1071 1012 467 449 346 335 246
190 177 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 866 2005 866
Size: 1139 5022
BtgI C'CryG_G
Cuts at: 282 2391 282
Size: 2109 4052
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4111 4292 4455 4111
Size: 181 163 5817
Fragments arranged by size:
5817 181 163
BtsI GCAGTG_nn'
Cuts at: 1636 2817 3404 1636
Size: 1181 587 4393
Fragments arranged by size:
4393 1181 587
ClaI AT'CG_AT
Cuts at: 1394 1394
Size: 6161
DraI TTT'AAA
Cuts at: 2870 3160 2870
Size: 290 5871
DraIII CAC_nnn'GTG
Cuts at: 2812 2812
Size: 6161
DrdI GACnn_nn'nnGTC
Cuts at: 1305 2755 3867 4725 1305
Size: 1450 1112 858 2741
Fragments arranged by size:
2741 1450 1112 858
EaeI y'GGCC_r
Cuts at: 258 698 1025 1115 2336 3746 3920 4311 4338
Size: 440 327 90 1221 1410 174 391 27
Cuts at: 4338 5683 258
Size: 1345 736
Fragments arranged by size:
1410 1345 1221 736 440 391 327 174
90 27
EagI C'GGCC_G
Cuts at: 1115 3746 1115
Size: 2631 3530
EarI CTCTTCn'nnn_
Cuts at: 383 947 2134 2240 4184 4394 383
Size: 564 1187 106 1944 210 2150
Fragments arranged by size:
2150 1944 1187 564 210 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 914 2957 4369 4679 4825 5971 914
Size: 2043 1412 310 146 1146 1104
Fragments arranged by size:
2043 1412 1146 1104 310 146
EcoICRI GAG'CTC
Cuts at: 342 990 1140 2460 2996 3173 342
Size: 648 150 1320 536 177 3330
Fragments arranged by size:
3330 1320 648 536 177 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 310 394 469 565 664 751 763 814
Size: 177 84 75 96 99 87 12 51
Cuts at: 814 1180 1261 1704 1791 1844 1999 2023 2071
Size: 366 81 443 87 53 155 24 48
Cuts at: 2071 2085 2377 2392 2509 2658 2782 2797 3986
Size: 14 292 15 117 149 124 15 1189
Cuts at: 3986 4418 5165 5433 133
Size: 432 747 268 861
Fragments arranged by size:
1189 861 747 443 432 366 292 268
177 155 149 124 117 99 96 87
87 84 81 75 53 51 48 24
15 15 14 12
EcoNI CCTnn'n_nnAGG
Cuts at: 499 499
Size: 6161
EcoO109I rG'GnC_Cy
Cuts at: 666 862 1482 1500 1501 1725 2521 2757 3087
Size: 196 620 18 1 224 796 236 330
Cuts at: 3087 3300 666
Size: 213 3527
Fragments arranged by size:
3527 796 620 330 236 224 213 196
18 1
EcoRI G'AATT_C
Cuts at: 3165 3165
Size: 6161
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2133 2869 3129 4180 2133
Size: 736 260 1051 4114
Fragments arranged by size:
4114 1051 736 260
FspI TGC'GCA
Cuts at: 220 3942 220
Size: 3722 2439
HaeII r_GCGC'y
Cuts at: 234 978 1063 1203 2002 2160 2594 3595 3843
Size: 744 85 140 799 158 434 1001 248
Cuts at: 3843 4865 5844 234
Size: 1022 979 551
Fragments arranged by size:
1022 1001 979 799 744 551 434 248
158 140 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1198 1230 1681 1713 1765 1797 2244 2276 4003
Size: 32 451 32 52 32 447 32 1727
Cuts at: 4003 4033 4035 4065 1198
Size: 30 2 30 3294
Fragments arranged by size:
3294 1727 451 447 52 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3200 3200
Size: 6161
HindIII A'AGCT_T
Cuts at: 604 604
Size: 6161
KasI G'GCGC_C
Cuts at: 3591 3839 3591
Size: 248 5913
KpnI G_GTAC'C
Cuts at: 3181 3181
Size: 6161
MluI A'CGCG_T
Cuts at: 5594 5594
Size: 6161
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1046 1214 1344 4832 5016 1046
Size: 168 130 3488 184 2191
Fragments arranged by size:
3488 2191 184 168 130
MscI TGG'CCA
Cuts at: 1027 3922 1027
Size: 2895 3266
MslI CAynn'nnrTG
Cuts at: 322 848 1534 2086 2347 3068 4150 4277 322
Size: 526 686 552 261 721 1082 127 2206
Fragments arranged by size:
2206 1082 721 686 552 526 261 127
MspA1I CmG'CkG
Cuts at: 153 174 402 1145 1866 1977 2043 2297 2547
Size: 21 228 743 721 111 66 254 250
Cuts at: 2547 2616 2658 2733 2795 3272 3586 3946 4959
Size: 69 42 75 62 477 314 360 1013
Cuts at: 4959 5204 153
Size: 245 1110
Fragments arranged by size:
1110 1013 743 721 477 360 314 254
250 245 228 111 75 69 66 62
42 21
NaeI GCC'GGC
Cuts at: 4342 4342
Size: 6161
NarI GG'CG_CC
Cuts at: 3592 3840 3592
Size: 248 5913
NgoMIV G'CCGG_C
Cuts at: 4340 4340
Size: 6161
NotI GC'GGCC_GC
Cuts at: 1115 1115
Size: 6161
NspI r_CATG'y
Cuts at: 2085 3214 3714 4621 2085
Size: 1129 500 907 3625
Fragments arranged by size:
3625 1129 907 500
PasI CC'CwG_GG
Cuts at: 495 1290 1505 1656 2753 3094 495
Size: 795 215 151 1097 341 3562
Fragments arranged by size:
3562 1097 795 341 215 151
PciI A'CATG_T
Cuts at: 4617 4617
Size: 6161
PflMI CCAn_nnn'nTGG
Cuts at: 1968 1968
Size: 6161
PfoI T'CCnGG_A
Cuts at: 2378 2378
Size: 6161
PmeI GTTT'AAAC
Cuts at: 3160 3160
Size: 6161
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5300 5332 5300
Size: 32 6129
PpuMI rG'GwC_Cy
Cuts at: 666 862 1725 2757 3087 666
Size: 196 863 1032 330 3740
Fragments arranged by size:
3740 1032 863 330 196
PspOMI G'GGCC_C
Cuts at: 1500 2521 1500
Size: 1021 5140
PspXI vC'TCGA_Gb
Cuts at: 343 991 2203 343
Size: 648 1212 4301
Fragments arranged by size:
4301 1212 648
PstI C_TGCA'G
Cuts at: 158 209 440 944 1130 1166 1330 1556 1865
Size: 51 231 504 186 36 164 226 309
Cuts at: 1865 2027 2621 2657 2675 2717 2738 3208 3893
Size: 162 594 36 18 42 21 470 685
Cuts at: 3893 158
Size: 2426
Fragments arranged by size:
2426 685 594 504 470 309 231 226
186 164 162 51 42 36 36 21
18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6161
PvuII CAG'CTG
Cuts at: 153 174 1866 1977 2297 2616 2658 2733 2795
Size: 21 1692 111 320 319 42 75 62
Cuts at: 2795 3586 3946 153
Size: 791 360 2368
Fragments arranged by size:
2368 1692 791 360 320 319 111 75
62 42 21
RsrII CG'GwC_CG
Cuts at: 1317 4356 1317
Size: 3039 3122
SacI G_AGCT'C
Cuts at: 344 992 1142 2462 2998 3175 344
Size: 648 150 1320 536 177 3330
Fragments arranged by size:
3330 1320 648 536 177 150
SalI G'TCGA_C
Cuts at: 3198 3198
Size: 6161
SapI GCTCTTCn'nnn_
Cuts at: 383 2134 4184 4394 383
Size: 1751 2050 210 2150
Fragments arranged by size:
2150 2050 1751 210
SbfI CC_TGCA'GG
Cuts at: 3208 3208
Size: 6161
ScaI AGT'ACT
Cuts at: 5678 5678
Size: 6161
SfcI C'TryA_G
Cuts at: 33 154 205 436 940 1126 1162 1326 1552
Size: 121 51 231 504 186 36 164 226
Cuts at: 1552 1861 2023 2542 2586 2617 2653 2671 2713
Size: 309 162 519 44 31 36 18 42
Cuts at: 2713 2734 3204 3889 4882 5073 33
Size: 21 470 685 993 191 1121
Fragments arranged by size:
1121 993 685 519 504 470 309 231
226 191 186 164 162 121 51 44
42 36 36 31 21 18
SfoI GGC'GCC
Cuts at: 3593 3841 3593
Size: 248 5913
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6161
SmaI CCC'GGG
Cuts at: 2953 3183 2953
Size: 230 5931
SmlI C'TyrA_G
Cuts at: 343 991 2152 2203 2461 3316 4231 4723 4985
Size: 648 1161 51 258 855 915 492 262
Cuts at: 4985 343
Size: 1519
Fragments arranged by size:
1519 1161 915 855 648 492 262 258
51
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6161
SphI G_CATG'C
Cuts at: 3214 3714 3214
Size: 500 5661
StuI AGG'CCT
Cuts at: 812 1812 2281 812
Size: 1000 469 4692
Fragments arranged by size:
4692 1000 469
StyI C'CwwG_G
Cuts at: 611 1646 1692 2423 2429 3026 3295 3649 611
Size: 1035 46 731 6 597 269 354 3123
Fragments arranged by size:
3123 1035 731 597 354 269 46 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 471 739 883 3058 3744 3877 4336 4545 471
Size: 268 144 2175 686 133 459 209 2087
Fragments arranged by size:
2175 2087 686 459 268 209 144 133
TatI w'GTAC_w
Cuts at: 2228 2321 5676 2228
Size: 93 3355 2713
Fragments arranged by size:
3355 2713 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1441 1473 5678 5710 1441
Size: 32 4205 32 1892
Fragments arranged by size:
4205 1892 32 32
Tth111I GACn'n_nGTC
Cuts at: 3958 3958
Size: 6161
XbaI T'CTAG_A
Cuts at: 3192 3192
Size: 6161
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1398 3656 1398
Size: 2258 3903
XhoI C'TCGA_G
Cuts at: 343 991 2203 343
Size: 648 1212 4301
Fragments arranged by size:
4301 1212 648
XmaI C'CCGG_G
Cuts at: 2951 3181 2951
Size: 230 5931
XmnI GAAnn'nnTTC
Cuts at: 392 1570 392
Size: 1178 4983
ZraI GAC'GTC
Cuts at: 126 126
Size: 6161
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsrGI BstEII BstXI BstZ17I CspCI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NruI NsiI
PacI PmlI PshAI PsiI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI