Restriction map for
pF1KB7340
(Circular) (Six-base) MAPSORT of: pF1KB7340.seq Check: 6075 from: 1 to: 6581
pF1KB7340 6581 bp
With 182 enzymes: SgfI *
August 20, 2009 16:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1176 1176
Size: 6581
AccI GT'mk_AC
Cuts at: 743 1820 1964 2620 3619 743
Size: 1077 144 656 999 3705
Fragments arranged by size:
3705 1077 999 656 144
Acc65I G'GTAC_C
Cuts at: 3597 3597
Size: 6581
AclI AA'CG_TT
Cuts at: 6421 6421
Size: 6581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 693 1847 2885 4406 4838 5585 5853 269
Size: 424 1154 1038 1521 432 747 268 997
Fragments arranged by size:
1521 1154 1038 997 747 432 424 268
AfeI AGC'GCT
Cuts at: 1050 1050
Size: 6581
AflIII A'CryG_T
Cuts at: 3555 3933 5037 6014 3555
Size: 378 1104 977 4122
Fragments arranged by size:
4122 1104 977 378
AgeI A'CCGG_T
Cuts at: 4969 4969
Size: 6581
AleI CACnn'nnGTG
Cuts at: 2149 2524 2149
Size: 375 6206
AlwNI CAG_nnn'CTG
Cuts at: 1082 2099 2913 3886 5453 1082
Size: 1017 814 973 1567 2210
Fragments arranged by size:
2210 1567 1017 973 814
ApaI G_GGCC'C
Cuts at: 567 578 2436 567
Size: 11 1858 4712
Fragments arranged by size:
4712 1858 11
ApaLI G'TGCA_C
Cuts at: 5351 5351
Size: 6581
ApoI r'AATT_y
Cuts at: 127 1752 1911 2050 2425 3396 3502 3585 5934
Size: 1625 159 139 375 971 106 83 2349
Cuts at: 5934 6190 127
Size: 256 518
Fragments arranged by size:
2349 1625 971 518 375 256 159 139
106 83
AseI AT'TA_AT
Cuts at: 20 20
Size: 6581
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6581
AvaI C'yCGr_G
Cuts at: 136 194 332 423 1947 3601 136
Size: 58 138 91 1524 1654 3116
Fragments arranged by size:
3116 1654 1524 138 91 58
AvrII C'CTAG_G
Cuts at: 975 975
Size: 6581
BamHI G'GATC_C
Cuts at: 452 600 2788 3606 452
Size: 148 2188 818 3427
Fragments arranged by size:
3427 2188 818 148
BanI G'GyrC_C
Cuts at: 140 175 685 1943 2370 3424 3597 4011 4259
Size: 35 510 1258 427 1054 173 414 248
Cuts at: 4259 4294 140
Size: 35 2427
Fragments arranged by size:
2427 1258 1054 510 427 414 248 173
35 35
BanII G_rGCy'C
Cuts at: 222 567 578 802 1225 1903 2436 3595 4625
Size: 345 11 224 423 678 533 1159 1030
Cuts at: 4625 222
Size: 2178
Fragments arranged by size:
2178 1159 1030 678 533 423 345 224
11
BbeI G_GCGC'C
Cuts at: 179 4015 4263 179
Size: 3836 248 2497
Fragments arranged by size:
3836 2497 248
BbsI GAAGACnn'nnnn_
Cuts at: 400 400
Size: 6581
BbvCI CC'TCA_GC
Cuts at: 2042 2709 2042
Size: 667 5914
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 285 319 1089 1123 285
Size: 34 770 34 5743
Fragments arranged by size:
5743 770 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1836 4479 5240 6581 1836
Size: 2643 761 1341 1836
Fragments arranged by size:
2643 1836 1341 761
BclI T'GATC_A
Cuts at: 1530 4101 1530
Size: 2571 4010
BfrBI ATG'CAT
Cuts at: 2208 2208
Size: 6581
BglII A'GATC_T
Cuts at: 4096 6133 4096
Size: 2037 4544
BlpI GC'TnA_GC
Cuts at: 1172 2990 3693 1172
Size: 1818 703 4060
Fragments arranged by size:
4060 1818 703
Bme1580I G_kGCm'C
Cuts at: 567 578 688 855 2373 2436 2475 2977 4206
Size: 11 110 167 1518 63 39 502 1229
Cuts at: 4206 4299 5355 567
Size: 93 1056 1793
Fragments arranged by size:
1793 1518 1229 1056 502 167 110 93
63 39 11
BmgBI CAC'GTC
Cuts at: 699 1760 699
Size: 1061 5520
BmrI ACTGGGnnnn_n'
Cuts at: 434 2087 2462 3074 3188 3793 3874 4209 6353
Size: 1653 375 612 114 605 81 335 2144
Cuts at: 6353 434
Size: 662
Fragments arranged by size:
2144 1653 662 612 605 375 335 114
81
BmtI G_CTAG'C
Cuts at: 3329 3329
Size: 6581
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1070 4948 1070
Size: 3878 2703
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 751 776 1875 2117 2492 2616 3101 565
Size: 186 25 1099 242 375 124 485 4045
Fragments arranged by size:
4045 1099 485 375 242 186 124 25
Bpu10I CC'TnA_GC
Cuts at: 646 896 1885 2042 2110 2485 2709 646
Size: 250 989 157 68 375 224 4518
Fragments arranged by size:
4518 989 375 250 224 157 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4636 5128 5426 3757
Size: 879 492 298 4912
Fragments arranged by size:
4912 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 544 1193 1261 1371 2745 6285 544
Size: 649 68 110 1374 3540 840
Fragments arranged by size:
3540 1374 840 649 110 68
BsaAI yAC'GTr
Cuts at: 499 3934 499
Size: 3435 3146
BsaBI GATnn'nnATC
Cuts at: 6 599 4095 6132 6
Size: 593 3496 2037 455
Fragments arranged by size:
3496 2037 593 455
BsaHI Gr'CG_yC
Cuts at: 176 200 787 4012 4260 4962 6458 176
Size: 24 587 3225 248 702 1496 299
Fragments arranged by size:
3225 1496 702 587 299 248 24
BsaWI w'CCGG_w
Cuts at: 603 876 1000 3573 3770 4291 4969 5243 5390
Size: 273 124 2573 197 521 678 274 147
Cuts at: 5390 5801 603
Size: 411 1383
Fragments arranged by size:
2573 1383 678 521 411 274 273 197
147 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 688 718 2202 2232 2577 2607 688
Size: 30 1484 30 345 30 4662
Fragments arranged by size:
4662 1484 345 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 449 562 1046 1481 1670 1673 2120 2207 2656
Size: 113 484 435 189 3 447 87 449
Cuts at: 2656 449
Size: 4374
Fragments arranged by size:
4374 484 449 447 435 189 113 87
3
BseYI C'CCAG_C
Cuts at: 463 531 826 967 1292 2311 2871 3244 4006
Size: 68 295 141 325 1019 560 373 762
Cuts at: 4006 4764 5341 5837 463
Size: 758 577 496 1207
Fragments arranged by size:
1207 1019 762 758 577 560 496 373
325 295 141 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1342 1952 2339 2720 3296 628
Size: 714 610 387 381 576 3913
Fragments arranged by size:
3913 714 610 576 387 381
BsiEI CG_ry'CG
Cuts at: 104 216 344 4123 4169 5377 104
Size: 112 128 3779 46 1208 1308
Fragments arranged by size:
3779 1308 1208 128 112 46
BsiHKAI G_wGCw'C
Cuts at: 802 1903 3595 4373 4563 5355 802
Size: 1101 1692 778 190 792 2028
Fragments arranged by size:
2028 1692 1101 792 778 190
BsmI GAATG_Cn'
Cuts at: 1471 1471
Size: 6581
BsmBI CGTCTCn'nnnn_
Cuts at: 689 794 689
Size: 105 6476
Bsp1286I G_dGCh'C
Cuts at: 222 567 578 688 802 855 1225 1903 2373
Size: 345 11 110 114 53 370 678 470
Cuts at: 2373 2436 2475 2977 3595 4206 4299 4373 4563
Size: 63 39 502 618 611 93 74 190
Cuts at: 4563 4625 5355 222
Size: 62 730 1448
Fragments arranged by size:
1448 730 678 618 611 502 470 370
345 190 114 110 93 74 63 62
53 39 11
BspEI T'CCGG_A
Cuts at: 876 1000 5801 876
Size: 124 4801 1656
Fragments arranged by size:
4801 1656 124
BspHI T'CATG_A
Cuts at: 5757 5757
Size: 6581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1176 3631 3845 4147 4528 1176
Size: 2455 214 302 381 3229
Fragments arranged by size:
3229 2455 381 302 214
BsrBI CCG'CTC
Cuts at: 50 184 4873 4927 6411 6576 50
Size: 134 4689 54 1484 165 55
Fragments arranged by size:
4689 1484 165 134 55 54
BsrDI GCAATG_nn'
Cuts at: 1105 1135 1923 2702 4493 1105
Size: 30 788 779 1791 3193
Fragments arranged by size:
3193 1791 788 779 30
BsrFI r'CCGG_y
Cuts at: 149 363 504 4579 4760 4969 149
Size: 214 141 4075 181 209 1761
Fragments arranged by size:
4075 1761 214 209 181 141
BssSI C'ACGA_G
Cuts at: 4852 5210 4852
Size: 358 6223
BstAPI GCAn_nnn'nTGC
Cuts at: 3958 3958
Size: 6581
BstBI TT'CG_AA
Cuts at: 4942 4942
Size: 6581
BstXI CCAn_nnnn'nTGG
Cuts at: 543 1142 2023 3529 543
Size: 599 881 1506 3595
Fragments arranged by size:
3595 1506 881 599
BstYI r'GATC_y
Cuts at: 1 452 600 2788 3430 3606 4073 4096 4431
Size: 451 148 2188 642 176 467 23 335
Cuts at: 4431 4677 5689 5775 5787 6133 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2188 1012 642 467 451 449 346 335
246 176 148 86 23 12
BtgI C'CryG_G
Cuts at: 107 249 107
Size: 142 6439
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4531 4712 4875 4531
Size: 181 163 6237
Fragments arranged by size:
6237 181 163
BtsI GCAGTG_nn'
Cuts at: 1129 1935 2321 2538 3411 3824 1129
Size: 806 386 217 873 413 3886
Fragments arranged by size:
3886 873 806 413 386 217
DraI TTT'AAA
Cuts at: 2399 2780 3580 2399
Size: 381 800 5400
Fragments arranged by size:
5400 800 381
DraIII CAC_nnn'GTG
Cuts at: 499 2917 499
Size: 2418 4163
DrdI GACnn_nn'nnGTC
Cuts at: 4287 5145 4287
Size: 858 5723
EaeI y'GGCC_r
Cuts at: 341 383 3294 4166 4340 4731 4758 6103 341
Size: 42 2911 872 174 391 27 1345 819
Fragments arranged by size:
2911 1345 872 819 391 174 42 27
EagI C'GGCC_G
Cuts at: 341 4166 341
Size: 3825 2756
EarI CTCTTCn'nnn_
Cuts at: 576 950 1213 1505 1706 4604 4814 576
Size: 374 263 292 201 2898 210 2343
Fragments arranged by size:
2898 2343 374 292 263 210 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 250 4789 5099 5245 6391 250
Size: 4539 310 146 1146 440
Fragments arranged by size:
4539 1146 440 310 146
EcoICRI GAG'CTC
Cuts at: 800 1901 3593 800
Size: 1101 1692 3788
Fragments arranged by size:
3788 1692 1101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 565 693 751 776 1847 1875 2117 2492
Size: 296 128 58 25 1071 28 242 375
Cuts at: 2492 2616 2885 3101 4406 4838 5585 5853 269
Size: 124 269 216 1305 432 747 268 997
Fragments arranged by size:
1305 1071 997 747 432 375 296 269
268 242 216 128 124 58 28 25
EcoNI CCTnn'n_nnAGG
Cuts at: 1086 1086
Size: 6581
EcoO109I rG'GnC_Cy
Cuts at: 357 574 638 677 814 1079 1724 2432 2433
Size: 217 64 39 137 265 645 708 1
Cuts at: 2433 3136 3190 3285 3445 3720 357
Size: 703 54 95 160 275 3218
Fragments arranged by size:
3218 708 703 645 275 265 217 160
137 95 64 54 39 1
EcoRI G'AATT_C
Cuts at: 1752 3396 3585 1752
Size: 1644 189 4748
Fragments arranged by size:
4748 1644 189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4600 4600
Size: 6581
FspI TGC'GCA
Cuts at: 4362 4362
Size: 6581
HaeII r_GCGC'y
Cuts at: 179 892 1052 1582 4015 4263 5285 6264 179
Size: 713 160 530 2433 248 1022 979 496
Fragments arranged by size:
2433 1022 979 713 530 496 248 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 306 338 444 476 688 720 1403 1435 2938
Size: 32 106 32 212 32 683 32 1503
Cuts at: 2938 2970 4423 4453 4455 4485 306
Size: 32 1453 30 2 30 2402
Fragments arranged by size:
2402 1503 1453 683 212 106 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2951 3620 2951
Size: 669 5912
HindIII A'AGCT_T
Cuts at: 2081 2081
Size: 6581
HpaI GTT'AAC
Cuts at: 2951 2951
Size: 6581
KasI G'GCGC_C
Cuts at: 175 4011 4259 175
Size: 3836 248 2497
Fragments arranged by size:
3836 2497 248
KpnI G_GTAC'C
Cuts at: 3601 3601
Size: 6581
MluI A'CGCG_T
Cuts at: 6014 6014
Size: 6581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2411 3081 3420 3591 5252 5436 2411
Size: 670 339 171 1661 184 3556
Fragments arranged by size:
3556 1661 670 339 184 171
MscI TGG'CCA
Cuts at: 385 3296 4342 385
Size: 2911 1046 2624
Fragments arranged by size:
2911 2624 1046
MslI CAynn'nnrTG
Cuts at: 1140 1928 2149 2524 3067 3527 4570 4697 1140
Size: 788 221 375 543 460 1043 127 3024
Fragments arranged by size:
3024 1043 788 543 460 375 221 127
MspA1I CmG'CkG
Cuts at: 251 653 993 1119 2315 3692 4006 4366 5379
Size: 402 340 126 1196 1377 314 360 1013
Cuts at: 5379 5624 251
Size: 245 1208
Fragments arranged by size:
1377 1208 1196 1013 402 360 340 314
245 126
NaeI GCC'GGC
Cuts at: 151 365 506 4762 151
Size: 214 141 4256 1970
Fragments arranged by size:
4256 1970 214 141
NarI GG'CG_CC
Cuts at: 176 4012 4260 176
Size: 3836 248 2497
Fragments arranged by size:
3836 2497 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 6581
NdeI CA'TA_TG
Cuts at: 3543 3543
Size: 6581
NgoMIV G'CCGG_C
Cuts at: 149 363 504 4760 149
Size: 214 141 4256 1970
Fragments arranged by size:
4256 1970 214 141
NheI G'CTAG_C
Cuts at: 3325 3325
Size: 6581
NotI GC'GGCC_GC
Cuts at: 341 341
Size: 6581
NsiI A_TGCA'T
Cuts at: 2210 2210
Size: 6581
NspI r_CATG'y
Cuts at: 474 3180 3559 3634 4134 5041 474
Size: 2706 379 75 500 907 2014
Fragments arranged by size:
2706 2014 907 500 379 75
PasI CC'CwG_GG
Cuts at: 681 690 721 681
Size: 9 31 6541
Fragments arranged by size:
6541 31 9
PciI A'CATG_T
Cuts at: 3555 5037 3555
Size: 1482 5099
PflMI CCAn_nnn'nTGG
Cuts at: 172 907 2917 3303 172
Size: 735 2010 386 3450
Fragments arranged by size:
3450 2010 735 386
PfoI T'CCnGG_A
Cuts at: 542 2403 2632 542
Size: 1861 229 4491
Fragments arranged by size:
4491 1861 229
PmeI GTTT'AAAC
Cuts at: 3580 3580
Size: 6581
PmlI CAC'GTG
Cuts at: 499 499
Size: 6581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 2178 5720 5752 2146
Size: 32 3542 32 2975
Fragments arranged by size:
3542 2975 32 32
PpuMI rG'GwC_Cy
Cuts at: 357 638 677 1724 3136 3285 357
Size: 281 39 1047 1412 149 3653
Fragments arranged by size:
3653 1412 1047 281 149 39
PshAI GACnn'nnGTC
Cuts at: 1960 1960
Size: 6581
PsiI TTA'TAA
Cuts at: 1559 1559
Size: 6581
PspOMI G'GGCC_C
Cuts at: 563 574 2432 563
Size: 11 1858 4712
Fragments arranged by size:
4712 1858 11
PstI C_TGCA'G
Cuts at: 926 2544 3482 3628 4313 926
Size: 1618 938 146 685 3194
Fragments arranged by size:
3194 1618 938 685 146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6581
PvuII CAG'CTG
Cuts at: 4006 4366 4006
Size: 360 6221
RsrII CG'GwC_CG
Cuts at: 4776 4776
Size: 6581
SacI G_AGCT'C
Cuts at: 802 1903 3595 802
Size: 1101 1692 3788
Fragments arranged by size:
3788 1692 1101
SacII CC_GC'GG
Cuts at: 252 252
Size: 6581
SalI G'TCGA_C
Cuts at: 3618 3618
Size: 6581
SanDI GG'GwC_CC
Cuts at: 3285 3285
Size: 6581
SapI GCTCTTCn'nnn_
Cuts at: 950 1213 1505 4604 4814 950
Size: 263 292 3099 210 2717
Fragments arranged by size:
3099 2717 292 263 210
SbfI CC_TGCA'GG
Cuts at: 3628 3628
Size: 6581
ScaI AGT'ACT
Cuts at: 6098 6098
Size: 6581
SexAI A'CCwGG_T
Cuts at: 2934 2934
Size: 6581
SfcI C'TryA_G
Cuts at: 33 922 2165 2378 2540 3478 3624 4309 5302
Size: 889 1243 213 162 938 146 685 993
Cuts at: 5302 5493 33
Size: 191 1121
Fragments arranged by size:
1243 1121 993 938 889 685 213 191
162 146
SfoI GGC'GCC
Cuts at: 177 4013 4261 177
Size: 3836 248 2497
Fragments arranged by size:
3836 2497 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6581
SmaI CCC'GGG
Cuts at: 138 196 1949 3603 138
Size: 58 1753 1654 3116
Fragments arranged by size:
3116 1753 1654 58
SmlI C'TyrA_G
Cuts at: 3736 4651 5143 5405 3736
Size: 915 492 262 4912
Fragments arranged by size:
4912 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6581
SphI G_CATG'C
Cuts at: 3634 4134 3634
Size: 500 6081
StuI AGG'CCT
Cuts at: 282 282
Size: 6581
StyI C'CwwG_G
Cuts at: 107 975 1042 1211 2225 2334 3715 4069 107
Size: 868 67 169 1014 109 1381 354 2619
Fragments arranged by size:
2619 1381 1014 868 354 169 109 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 414 568 703 1306 1516 3258 3535 4164 4297
Size: 154 135 603 210 1742 277 629 133
Cuts at: 4297 4756 4965 414
Size: 459 209 2030
Fragments arranged by size:
2030 1742 629 603 459 277 210 209
154 135 133
TatI w'GTAC_w
Cuts at: 2645 6096 2645
Size: 3451 3130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 427 459 2715 2747 6098 6130 427
Size: 32 2256 32 3351 32 878
Fragments arranged by size:
3351 2256 878 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4378 4378
Size: 6581
XbaI T'CTAG_A
Cuts at: 3612 3612
Size: 6581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1389 1452 2914 4076 1389
Size: 63 1462 1162 3894
Fragments arranged by size:
3894 1462 1162 63
XmaI C'CCGG_G
Cuts at: 136 194 1947 3601 136
Size: 58 1753 1654 3116
Fragments arranged by size:
3116 1753 1654 58
XmnI GAAnn'nnTTC
Cuts at: 1027 1027
Size: 6581
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI BaeI BglI
BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI MfeI NruI PacI PspXI PsrI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI