Restriction map for
pF1KB7335
(Circular) (Six-base) MAPSORT of: pF1KB7335.seq Check: 7334 from: 1 to: 6173
pF1KB7335 6173 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 141 3067 141
Size: 2926 3247
AccI GT'mk_AC
Cuts at: 1127 3211 1127
Size: 2084 4089
Acc65I G'GTAC_C
Cuts at: 351 3189 351
Size: 2838 3335
AclI AA'CG_TT
Cuts at: 6013 6013
Size: 6173
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1621 2597 2612 2705 3998 4430 5177 5445
Size: 1374 976 15 93 1293 432 747 268
Cuts at: 5445 247
Size: 975
Fragments arranged by size:
1374 1293 976 975 747 432 268 93
15
AfeI AGC'GCT
Cuts at: 2497 2497
Size: 6173
AflIII A'CryG_T
Cuts at: 3525 4629 5606 3525
Size: 1104 977 4092
Fragments arranged by size:
4092 1104 977
AgeI A'CCGG_T
Cuts at: 4561 4561
Size: 6173
AleI CACnn'nnGTG
Cuts at: 1732 2699 2827 1732
Size: 967 128 5078
Fragments arranged by size:
5078 967 128
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 314 346 314
Size: 32 6141
AlwNI CAG_nnn'CTG
Cuts at: 609 1657 2304 2895 3478 5045 609
Size: 1048 647 591 583 1567 1737
Fragments arranged by size:
1737 1567 1048 647 591 583
ApaI G_GGCC'C
Cuts at: 1468 1468
Size: 6173
ApaLI G'TGCA_C
Cuts at: 1532 4943 1532
Size: 3411 2762
ApoI r'AATT_y
Cuts at: 1818 3177 5526 5782 1818
Size: 1359 2349 256 2209
Fragments arranged by size:
2349 2209 1359 256
AseI AT'TA_AT
Cuts at: 20 1042 20
Size: 1022 5151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6173
AvaI C'yCGr_G
Cuts at: 362 1469 1902 3193 362
Size: 1107 433 1291 3342
Fragments arranged by size:
3342 1291 1107 433
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 896 929 2968 3001 896
Size: 33 2039 33 4068
Fragments arranged by size:
4068 2039 33 33
BamHI G'GATC_C
Cuts at: 3198 3198
Size: 6173
BanI G'GyrC_C
Cuts at: 351 1290 1440 2391 3189 3603 3851 3886 351
Size: 939 150 951 798 414 248 35 2638
Fragments arranged by size:
2638 951 939 798 414 248 150 35
BanII G_rGCy'C
Cuts at: 1468 2813 3187 4217 1468
Size: 1345 374 1030 3424
Fragments arranged by size:
3424 1345 1030 374
BbeI G_GCGC'C
Cuts at: 3607 3855 3607
Size: 248 5925
BbsI GAAGACnn'nnnn_
Cuts at: 749 749
Size: 6173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1531 1565 1828 1862 1531
Size: 34 263 34 5842
Fragments arranged by size:
5842 263 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4071 4832 6173 4071
Size: 761 1341 4071
Fragments arranged by size:
4071 1341 761
BclI T'GATC_A
Cuts at: 890 3693 890
Size: 2803 3370
BfrBI ATG'CAT
Cuts at: 1845 2090 1845
Size: 245 5928
BglII A'GATC_T
Cuts at: 452 551 3688 5725 452
Size: 99 3137 2037 900
Fragments arranged by size:
3137 2037 900 99
BlpI GC'TnA_GC
Cuts at: 2448 2959 3285 2448
Size: 511 326 5336
Fragments arranged by size:
5336 511 326
Bme1580I G_kGCm'C
Cuts at: 1031 1468 1536 2034 2394 3798 3891 4947 1031
Size: 437 68 498 360 1404 93 1056 2257
Fragments arranged by size:
2257 1404 1056 498 437 360 93 68
BmrI ACTGGGnnnn_n'
Cuts at: 781 1745 1770 3385 3466 3801 5945 781
Size: 964 25 1615 81 335 2144 1009
Fragments arranged by size:
2144 1615 1009 964 335 81 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1717 2820 4540 1717
Size: 1103 1720 3350
Fragments arranged by size:
3350 1720 1103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1474 1700 1729 1885 1998 2010 2662 2815 2872
Size: 226 29 156 113 12 652 153 57
Cuts at: 2872 3106 1474
Size: 234 4541
Fragments arranged by size:
4541 652 234 226 156 153 113 57
29 12
Bpu10I CC'TnA_GC
Cuts at: 412 492 1094 1658 412
Size: 80 602 564 4927
Fragments arranged by size:
4927 602 564 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2178 3349 4228 4720 5018 2178
Size: 1171 879 492 298 3333
Fragments arranged by size:
3333 1171 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1236 3033 5877 1236
Size: 1797 2844 1532
Fragments arranged by size:
2844 1797 1532
BsaAI yAC'GTr
Cuts at: 3526 3526
Size: 6173
BsaBI GATnn'nnATC
Cuts at: 6 3687 5724 6
Size: 3681 2037 455
Fragments arranged by size:
3681 2037 455
BsaHI Gr'CG_yC
Cuts at: 788 2467 3604 3852 4554 6050 788
Size: 1679 1137 248 702 1496 911
Fragments arranged by size:
1679 1496 1137 911 702 248
BsaWI w'CCGG_w
Cuts at: 3362 3883 4561 4835 4982 5393 3362
Size: 521 678 274 147 411 4142
Fragments arranged by size:
4142 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 442 472 2722 2752 2830 2860 442
Size: 30 2250 30 78 30 3755
Fragments arranged by size:
3755 2250 78 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 567 1007 1135 1638 1941 2551 3065 567
Size: 440 128 503 303 610 514 3675
Fragments arranged by size:
3675 610 514 503 440 303 128
BseYI C'CCAG_C
Cuts at: 145 638 2286 2555 3598 4356 4933 5429 145
Size: 493 1648 269 1043 758 577 496 889
Fragments arranged by size:
1648 1043 889 758 577 496 493 269
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 256 896 1822 1956 208
Size: 48 640 926 134 4425
Fragments arranged by size:
4425 926 640 134 48
BsiEI CG_ry'CG
Cuts at: 104 3715 3761 4969 104
Size: 3611 46 1208 1308
Fragments arranged by size:
3611 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1226 1536 1739 2813 3187 3965 4155 4947 1226
Size: 310 203 1074 374 778 190 792 2452
Fragments arranged by size:
2452 1074 792 778 374 310 203 190
BsiWI C'GTAC_G
Cuts at: 1320 1320
Size: 6173
BsmBI CGTCTCn'nnnn_
Cuts at: 795 795
Size: 6173
Bsp1286I G_dGCh'C
Cuts at: 1031 1226 1468 1536 1739 2034 2394 2813 3187
Size: 195 242 68 203 295 360 419 374
Cuts at: 3187 3798 3891 3965 4155 4217 4947 1031
Size: 611 93 74 190 62 730 2257
Fragments arranged by size:
2257 730 611 419 374 360 295 242
203 195 190 93 74 68 62
BspEI T'CCGG_A
Cuts at: 5393 5393
Size: 6173
BspHI T'CATG_A
Cuts at: 5349 5349
Size: 6173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 141 3067 3223 3437 3739 4120 141
Size: 2926 156 214 302 381 2194
Fragments arranged by size:
2926 2194 381 302 214 156
BsrBI CCG'CTC
Cuts at: 50 1691 2447 4465 4519 6003 6168 50
Size: 1641 756 2018 54 1484 165 55
Fragments arranged by size:
2018 1641 1484 756 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1064 1917 2549 4085 1064
Size: 853 632 1536 3152
Fragments arranged by size:
3152 1536 853 632
BsrFI r'CCGG_y
Cuts at: 2130 2312 4171 4352 4561 2130
Size: 182 1859 181 209 3742
Fragments arranged by size:
3742 1859 209 182 181
BssSI C'ACGA_G
Cuts at: 4444 4802 4444
Size: 358 5815
BstAPI GCAn_nnn'nTGC
Cuts at: 3550 3550
Size: 6173
BstBI TT'CG_AA
Cuts at: 4534 4534
Size: 6173
BstEII G'GTnAC_C
Cuts at: 366 903 366
Size: 537 5636
BstXI CCAn_nnnn'nTGG
Cuts at: 1753 1753
Size: 6173
BstYI r'GATC_y
Cuts at: 1 452 526 551 1940 2168 2909 3071 3198
Size: 451 74 25 1389 228 741 162 127
Cuts at: 3198 3665 3688 4023 4269 5281 5367 5379 5725
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5725 1
Size: 449
Fragments arranged by size:
1389 1012 741 467 451 449 346 335
246 228 162 127 86 74 25 23
12
Bsu36I CC'TnA_GG
Cuts at: 1460 1460
Size: 6173
BtgI C'CryG_G
Cuts at: 107 794 1808 2929 107
Size: 687 1014 1121 3351
Fragments arranged by size:
3351 1121 1014 687
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1038 1831 1845 4123 4304 4467 1038
Size: 793 14 2278 181 163 2744
Fragments arranged by size:
2744 2278 793 181 163 14
BtsI GCAGTG_nn'
Cuts at: 847 937 963 1170 2425 2619 3416 847
Size: 90 26 207 1255 194 797 3604
Fragments arranged by size:
3604 1255 797 207 194 90 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2414 2449 2414
Size: 35 6138
DraI TTT'AAA
Cuts at: 3172 3172
Size: 6173
DraIII CAC_nnn'GTG
Cuts at: 606 606
Size: 6173
DrdI GACnn_nn'nnGTC
Cuts at: 3061 3879 4737 3061
Size: 818 858 4497
Fragments arranged by size:
4497 858 818
EaeI y'GGCC_r
Cuts at: 698 1020 1361 3758 3932 4323 4350 5695 698
Size: 322 341 2397 174 391 27 1345 1176
Fragments arranged by size:
2397 1345 1176 391 341 322 174 27
EagI C'GGCC_G
Cuts at: 3758 3758
Size: 6173
EarI CTCTTCn'nnn_
Cuts at: 1708 2159 2460 2681 2725 4196 4406 1708
Size: 451 301 221 44 1471 210 3475
Fragments arranged by size:
3475 1471 451 301 221 210 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4381 4691 4837 5983 4381
Size: 310 146 1146 4571
Fragments arranged by size:
4571 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2811 3185 2811
Size: 374 5799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1474 1621 1700 1729 1885 1998 2010 2597
Size: 1227 147 79 29 156 113 12 587
Cuts at: 2597 2612 2662 2705 2815 2872 3106 3998 4430
Size: 15 50 43 110 57 234 892 432
Cuts at: 4430 5177 5445 247
Size: 747 268 975
Fragments arranged by size:
1227 975 892 747 587 432 268 234
156 147 113 110 79 57 50 43
29 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 685 3159 685
Size: 2474 3699
EcoO109I rG'GnC_Cy
Cuts at: 765 1073 1373 1464 1465 2082 2301 2790 2860
Size: 308 300 91 1 617 219 489 70
Cuts at: 2860 2892 3004 3312 765
Size: 32 112 308 3626
Fragments arranged by size:
3626 617 489 308 308 300 219 112
91 70 32 1
EcoRI G'AATT_C
Cuts at: 3177 3177
Size: 6173
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2394 2602 4192 2394
Size: 208 1590 4375
Fragments arranged by size:
4375 1590 208
FspI TGC'GCA
Cuts at: 1162 1420 3954 1162
Size: 258 2534 3381
Fragments arranged by size:
3381 2534 258
FspAI rTGC'GCAy
Cuts at: 1420 1420
Size: 6173
HaeII r_GCGC'y
Cuts at: 123 1920 2499 3607 3855 4877 5856 123
Size: 1797 579 1108 248 1022 979 440
Fragments arranged by size:
1797 1108 1022 979 579 440 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 518 550 792 824 2740 2772 4015 4045 4047
Size: 32 242 32 1916 32 1243 30 2
Cuts at: 4047 4077 518
Size: 30 2614
Fragments arranged by size:
2614 1916 1243 242 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3212 3212
Size: 6173
KasI G'GCGC_C
Cuts at: 3603 3851 3603
Size: 248 5925
KpnI G_GTAC'C
Cuts at: 355 3193 355
Size: 2838 3335
MfeI C'AATT_G
Cuts at: 1378 1378
Size: 6173
MluI A'CGCG_T
Cuts at: 5606 5606
Size: 6173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 386 2607 4844 5028 386
Size: 2221 2237 184 1531
Fragments arranged by size:
2237 2221 1531 184
MscI TGG'CCA
Cuts at: 700 1022 1363 3934 700
Size: 322 341 2571 2939
Fragments arranged by size:
2939 2571 341 322
MslI CAynn'nnrTG
Cuts at: 1057 1732 2632 2699 2827 4162 4289 1057
Size: 675 900 67 128 1335 127 2941
Fragments arranged by size:
2941 1335 900 675 128 127 67
MspA1I CmG'CkG
Cuts at: 488 642 805 2683 2982 3284 3598 3958 4971
Size: 154 163 1878 299 302 314 360 1013
Cuts at: 4971 5216 488
Size: 245 1445
Fragments arranged by size:
1878 1445 1013 360 314 302 299 245
163 154
NaeI GCC'GGC
Cuts at: 4354 4354
Size: 6173
NarI GG'CG_CC
Cuts at: 3604 3852 3604
Size: 248 5925
NcoI C'CATG_G
Cuts at: 107 794 1808 107
Size: 687 1014 4472
Fragments arranged by size:
4472 1014 687
NgoMIV G'CCGG_C
Cuts at: 4352 4352
Size: 6173
NsiI A_TGCA'T
Cuts at: 1847 2092 1847
Size: 245 5928
NspI r_CATG'y
Cuts at: 1419 1845 3226 3726 4633 1419
Size: 426 1381 500 907 2959
Fragments arranged by size:
2959 1381 907 500 426
PasI CC'CwG_GG
Cuts at: 304 304
Size: 6173
PciI A'CATG_T
Cuts at: 4629 4629
Size: 6173
PflMI CCAn_nnn'nTGG
Cuts at: 424 609 2848 424
Size: 185 2239 3749
Fragments arranged by size:
3749 2239 185
PfoI T'CCnGG_A
Cuts at: 1862 2888 1862
Size: 1026 5147
PmeI GTTT'AAAC
Cuts at: 3172 3172
Size: 6173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5312 5344 5312
Size: 32 6141
PpuMI rG'GwC_Cy
Cuts at: 765 1073 1373 2860 2892 3004 765
Size: 308 300 1487 32 112 3934
Fragments arranged by size:
3934 1487 308 300 112 32
PshAI GACnn'nnGTC
Cuts at: 594 594
Size: 6173
PspOMI G'GGCC_C
Cuts at: 1464 1464
Size: 6173
PspXI vC'TCGA_Gb
Cuts at: 1469 1469
Size: 6173
PstI C_TGCA'G
Cuts at: 596 659 713 854 1015 1601 2614 2682 3220
Size: 63 54 141 161 586 1013 68 538
Cuts at: 3220 3905 596
Size: 685 2864
Fragments arranged by size:
2864 1013 685 586 538 161 141 68
63 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6173
PvuII CAG'CTG
Cuts at: 805 2683 2982 3598 3958 805
Size: 1878 299 616 360 3020
Fragments arranged by size:
3020 1878 616 360 299
RsrII CG'GwC_CG
Cuts at: 4368 4368
Size: 6173
SacI G_AGCT'C
Cuts at: 2813 3187 2813
Size: 374 5799
SalI G'TCGA_C
Cuts at: 3210 3210
Size: 6173
SanDI GG'GwC_CC
Cuts at: 1373 1373
Size: 6173
SapI GCTCTTCn'nnn_
Cuts at: 4196 4406 4196
Size: 210 5963
SbfI CC_TGCA'GG
Cuts at: 3220 3220
Size: 6173
ScaI AGT'ACT
Cuts at: 5690 5690
Size: 6173
SexAI A'CCwGG_T
Cuts at: 602 1226 2418 602
Size: 624 1192 4357
Fragments arranged by size:
4357 1192 624
SfcI C'TryA_G
Cuts at: 33 592 655 709 850 1011 1338 1597 1920
Size: 559 63 54 141 161 327 259 323
Cuts at: 1920 2610 2678 3216 3901 4894 5085 33
Size: 690 68 538 685 993 191 1121
Fragments arranged by size:
1121 993 690 685 559 538 327 323
259 191 161 141 68 63 54
SfoI GGC'GCC
Cuts at: 3605 3853 3605
Size: 248 5925
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6173
SmaI CCC'GGG
Cuts at: 364 1904 3195 364
Size: 1540 1291 3342
Fragments arranged by size:
3342 1540 1291
SmlI C'TyrA_G
Cuts at: 1469 2157 3328 4243 4735 4997 1469
Size: 688 1171 915 492 262 2645
Fragments arranged by size:
2645 1171 915 688 492 262
SpeI A'CTAG_T
Cuts at: 67 1992 67
Size: 1925 4248
SphI G_CATG'C
Cuts at: 3226 3726 3226
Size: 500 5673
SspI AAT'ATT
Cuts at: 1189 1189
Size: 6173
StuI AGG'CCT
Cuts at: 571 571
Size: 6173
StyI C'CwwG_G
Cuts at: 107 334 794 1808 2078 2219 2378 2432 3307
Size: 227 460 1014 270 141 159 54 875
Cuts at: 3307 3661 107
Size: 354 2619
Fragments arranged by size:
2619 1014 875 460 354 270 227 159
141 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 296 2823 2942 3756 3889 4348 4557 296
Size: 2527 119 814 133 459 209 1912
Fragments arranged by size:
2527 1912 814 459 209 133 119
TatI w'GTAC_w
Cuts at: 2711 5688 2711
Size: 2977 3196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1624 1656 2722 2754 5690 5722 1624
Size: 32 1066 32 2936 32 2075
Fragments arranged by size:
2936 2075 1066 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1303 3970 1303
Size: 2667 3506
XbaI T'CTAG_A
Cuts at: 3204 3204
Size: 6173
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 955 3668 955
Size: 2713 3460
XhoI C'TCGA_G
Cuts at: 1469 1469
Size: 6173
XmaI C'CCGG_G
Cuts at: 362 1902 3193 362
Size: 1540 1291 3342
Fragments arranged by size:
3342 1540 1291
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AscI AvrII BbvCI BglI
BmgBI BmtI BsmI BsrGI BssHII BstZ17I ClaI EcoRV
FseI HindIII HpaI NdeI NheI NotI NruI PacI
PmlI PsiI PsrI SacII SfiI SgrAI SnaBI SrfI
SwaI XmnI ZraI