Restriction map for
pF1KB7332
(Circular) (Six-base) MAPSORT of: pF1KB7332.seq Check: 4311 from: 1 to: 6209
pF1KB7332 6209 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 788 3247 788
Size: 2459 3750
Acc65I G'GTAC_C
Cuts at: 2589 3225 2589
Size: 636 5573
AclI AA'CG_TT
Cuts at: 3027 6049 3027
Size: 3022 3187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 1354 1888 1900 2885 4034 4466 5213 5481
Size: 1022 534 12 985 1149 432 747 268
Cuts at: 5481 332
Size: 1060
Fragments arranged by size:
1149 1060 1022 985 747 534 432 268
12
AflIII A'CryG_T
Cuts at: 2133 3561 4665 5642 2133
Size: 1428 1104 977 2700
Fragments arranged by size:
2700 1428 1104 977
AgeI A'CCGG_T
Cuts at: 4597 4597
Size: 6209
AleI CACnn'nnGTG
Cuts at: 3040 3040
Size: 6209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2726 2726
Size: 6209
AlwNI CAG_nnn'CTG
Cuts at: 3514 5081 3514
Size: 1567 4642
ApaI G_GGCC'C
Cuts at: 247 753 247
Size: 506 5703
ApaLI G'TGCA_C
Cuts at: 4979 4979
Size: 6209
ApoI r'AATT_y
Cuts at: 676 687 844 1555 1891 2595 2802 3213 5562
Size: 11 157 711 336 704 207 411 2349
Cuts at: 5562 5818 676
Size: 256 1067
Fragments arranged by size:
2349 1067 711 704 411 336 256 207
157 11
AseI AT'TA_AT
Cuts at: 20 20
Size: 6209
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6209
AvaI C'yCGr_G
Cuts at: 361 1146 3229 361
Size: 785 2083 3341
Fragments arranged by size:
3341 2083 785
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2319 2352 2319
Size: 33 6176
BamHI G'GATC_C
Cuts at: 523 3234 523
Size: 2711 3498
BanI G'GyrC_C
Cuts at: 2589 3225 3639 3887 3922 2589
Size: 636 414 248 35 4876
Fragments arranged by size:
4876 636 414 248 35
BanII G_rGCy'C
Cuts at: 247 753 924 1278 2308 3223 4253 247
Size: 506 171 354 1030 915 1030 2203
Fragments arranged by size:
2203 1030 1030 915 506 354 171
BbeI G_GCGC'C
Cuts at: 3643 3891 3643
Size: 248 5961
BbsI GAAGACnn'nnnn_
Cuts at: 2077 2077
Size: 6209
BciVI GTATCCnnnnn_n'
Cuts at: 4107 4868 6209 4107
Size: 761 1341 4107
Fragments arranged by size:
4107 1341 761
BclI T'GATC_A
Cuts at: 353 3729 353
Size: 3376 2833
BfrBI ATG'CAT
Cuts at: 2116 2116
Size: 6209
BglII A'GATC_T
Cuts at: 1071 1596 3724 5761 1071
Size: 525 2128 2037 1519
Fragments arranged by size:
2128 2037 1519 525
BlpI GC'TnA_GC
Cuts at: 3321 3321
Size: 6209
Bme1580I G_kGCm'C
Cuts at: 247 614 753 1977 3834 3927 4983 247
Size: 367 139 1224 1857 93 1056 1473
Fragments arranged by size:
1857 1473 1224 1056 367 139 93
BmgBI CAC'GTC
Cuts at: 407 1734 407
Size: 1327 4882
BmrI ACTGGGnnnn_n'
Cuts at: 3421 3502 3837 5981 3421
Size: 81 335 2144 3649
Fragments arranged by size:
3649 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4576 4576
Size: 6209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 2126 2675 403
Size: 1723 549 3937
Fragments arranged by size:
3937 1723 549
Bpu10I CC'TnA_GC
Cuts at: 1489 1489
Size: 6209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2256 2893 3385 4264 4756 5054 2256
Size: 637 492 879 492 298 3411
Fragments arranged by size:
3411 879 637 492 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5913 5913
Size: 6209
BsaAI yAC'GTr
Cuts at: 3562 3562
Size: 6209
BsaBI GATnn'nnATC
Cuts at: 6 3723 5760 6
Size: 3717 2037 455
Fragments arranged by size:
3717 2037 455
BsaHI Gr'CG_yC
Cuts at: 3640 3888 4590 6086 3640
Size: 248 702 1496 3763
Fragments arranged by size:
3763 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2738 3398 3919 4597 4871 5018 5429 2738
Size: 660 521 678 274 147 411 3518
Fragments arranged by size:
3518 678 660 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1019 1049 1019
Size: 30 6179
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 914 932 914
Size: 18 6191
BseYI C'CCAG_C
Cuts at: 423 3634 4392 4969 5465 423
Size: 3211 758 577 496 1167
Fragments arranged by size:
3211 1167 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 1346 265
Size: 1081 5128
BsiEI CG_ry'CG
Cuts at: 104 197 201 275 3751 3797 5005 104
Size: 93 4 74 3476 46 1208 1308
Fragments arranged by size:
3476 1308 1208 93 74 46 4
BsiHKAI G_wGCw'C
Cuts at: 924 1089 1102 1278 2308 3223 4001 4191 4983
Size: 165 13 176 1030 915 778 190 792
Cuts at: 4983 924
Size: 2150
Fragments arranged by size:
2150 1030 915 792 778 190 176 165
13
BsmI GAATG_Cn'
Cuts at: 1167 2665 1167
Size: 1498 4711
BsmBI CGTCTCn'nnnn_
Cuts at: 154 154
Size: 6209
Bsp1286I G_dGCh'C
Cuts at: 247 614 753 924 1089 1102 1278 1977 2308
Size: 367 139 171 165 13 176 699 331
Cuts at: 2308 3223 3834 3927 4001 4191 4253 4983 247
Size: 915 611 93 74 190 62 730 1473
Fragments arranged by size:
1473 915 730 699 611 367 331 190
176 171 165 139 93 74 62 13
BspEI T'CCGG_A
Cuts at: 5429 5429
Size: 6209
BspHI T'CATG_A
Cuts at: 5385 5385
Size: 6209
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3259 3473 3775 4156 3259
Size: 214 302 381 5312
Fragments arranged by size:
5312 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2320 4501 4555 6039 6204 50
Size: 2270 2181 54 1484 165 55
Fragments arranged by size:
2270 2181 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1172 2720 3201 4121 1172
Size: 1548 481 920 3260
Fragments arranged by size:
3260 1548 920 481
BsrFI r'CCGG_y
Cuts at: 196 2870 4207 4388 4597 196
Size: 2674 1337 181 209 1808
Fragments arranged by size:
2674 1808 1337 209 181
BsrGI T'GTAC_A
Cuts at: 1130 1130
Size: 6209
BssHII G'CGCG_C
Cuts at: 227 227
Size: 6209
BssSI C'ACGA_G
Cuts at: 1078 4480 4838 1078
Size: 3402 358 2449
Fragments arranged by size:
3402 2449 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1459 3586 1459
Size: 2127 4082
BstBI TT'CG_AA
Cuts at: 4570 4570
Size: 6209
BstYI r'GATC_y
Cuts at: 1 523 1071 1334 1596 3234 3701 3724 4059
Size: 522 548 263 262 1638 467 23 335
Cuts at: 4059 4305 5317 5403 5415 5761 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1638 1012 548 522 467 449 346 335
263 262 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 288 2147 107
Size: 181 1859 4169
Fragments arranged by size:
4169 1859 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4159 4340 4503 4159
Size: 181 163 5865
Fragments arranged by size:
5865 181 163
BtsI GCAGTG_nn'
Cuts at: 323 3452 323
Size: 3129 3080
DraI TTT'AAA
Cuts at: 2417 2801 3123 3208 2417
Size: 384 322 85 5418
Fragments arranged by size:
5418 384 322 85
DrdI GACnn_nn'nnGTC
Cuts at: 3915 4773 3915
Size: 858 5351
EaeI y'GGCC_r
Cuts at: 198 399 1320 2872 3794 3968 4359 4386 5731
Size: 201 921 1552 922 174 391 27 1345
Cuts at: 5731 198
Size: 676
Fragments arranged by size:
1552 1345 922 921 676 391 201 174
27
EagI C'GGCC_G
Cuts at: 198 3794 198
Size: 3596 2613
EarI CTCTTCn'nnn_
Cuts at: 461 1113 1719 2177 2318 2360 3156 4232 4442
Size: 652 606 458 141 42 796 1076 210
Cuts at: 4442 461
Size: 2228
Fragments arranged by size:
2228 1076 796 652 606 458 210 141
42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4417 4727 4873 6019 4417
Size: 310 146 1146 4607
Fragments arranged by size:
4607 1146 310 146
EcoICRI GAG'CTC
Cuts at: 922 1276 2306 3221 922
Size: 354 1030 915 3910
Fragments arranged by size:
3910 1030 915 354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 403 1354 1888 1900 2126 2675 2885 4034
Size: 71 951 534 12 226 549 210 1149
Cuts at: 4034 4466 5213 5481 332
Size: 432 747 268 1060
Fragments arranged by size:
1149 1060 951 747 549 534 432 268
226 210 71 12
EcoO109I rG'GnC_Cy
Cuts at: 189 243 244 749 750 2328 3348 189
Size: 54 1 505 1 1578 1020 3050
Fragments arranged by size:
3050 1578 1020 505 54 1 1
EcoRI G'AATT_C
Cuts at: 1891 3213 1891
Size: 1322 4887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3132 4228 3132
Size: 1096 5113
FseI GG_CCGG'CC
Cuts at: 2874 2874
Size: 6209
FspI TGC'GCA
Cuts at: 2987 3990 2987
Size: 1003 5206
HaeII r_GCGC'y
Cuts at: 3643 3891 4913 5892 3643
Size: 248 1022 979 3960
Fragments arranged by size:
3960 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 368 400 462 494 762 794 854 886 1019
Size: 32 62 32 268 32 60 32 133
Cuts at: 1019 1051 1083 1321 1353 1358 1390 2254 2286
Size: 32 32 238 32 5 32 864 32
Cuts at: 2286 2290 2322 4051 4081 4083 4113 368
Size: 4 32 1729 30 2 30 2464
Fragments arranged by size:
2464 1729 864 268 238 133 62 60
32 32 32 32 32 32 32 32
32 32 30 30 5 4 2
HincII GTy'rAC
Cuts at: 1343 1515 3248 1343
Size: 172 1733 4304
Fragments arranged by size:
4304 1733 172
HindIII A'AGCT_T
Cuts at: 1009 1009
Size: 6209
KasI G'GCGC_C
Cuts at: 3639 3887 3639
Size: 248 5961
KpnI G_GTAC'C
Cuts at: 2593 3229 2593
Size: 636 5573
MluI A'CGCG_T
Cuts at: 5642 5642
Size: 6209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 852 894 1164 2883 3151 4880 5064 852
Size: 42 270 1719 268 1729 184 1997
Fragments arranged by size:
1997 1729 1719 270 268 184 42
MscI TGG'CCA
Cuts at: 3970 3970
Size: 6209
MslI CAynn'nnrTG
Cuts at: 1753 3040 4198 4325 1753
Size: 1287 1158 127 3637
Fragments arranged by size:
3637 1287 1158 127
MspA1I CmG'CkG
Cuts at: 423 3320 3634 3994 5007 5252 423
Size: 2897 314 360 1013 245 1380
Fragments arranged by size:
2897 1380 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2872 4390 2872
Size: 1518 4691
NarI GG'CG_CC
Cuts at: 3640 3888 3640
Size: 248 5961
NcoI C'CATG_G
Cuts at: 107 107
Size: 6209
NdeI CA'TA_TG
Cuts at: 2118 3096 2118
Size: 978 5231
NgoMIV G'CCGG_C
Cuts at: 2870 4388 2870
Size: 1518 4691
NsiI A_TGCA'T
Cuts at: 2118 2118
Size: 6209
NspI r_CATG'y
Cuts at: 2137 2350 2992 3262 3762 4669 2137
Size: 213 642 270 500 907 3677
Fragments arranged by size:
3677 907 642 500 270 213
PciI A'CATG_T
Cuts at: 2133 4665 2133
Size: 2532 3677
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 6209
PfoI T'CCnGG_A
Cuts at: 155 2294 155
Size: 2139 4070
PmeI GTTT'AAAC
Cuts at: 3208 3208
Size: 6209
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5348 5380 5348
Size: 32 6177
PpuMI rG'GwC_Cy
Cuts at: 189 2328 189
Size: 2139 4070
PsiI TTA'TAA
Cuts at: 1658 1658
Size: 6209
PspOMI G'GGCC_C
Cuts at: 243 749 243
Size: 506 5703
PstI C_TGCA'G
Cuts at: 2242 3256 3941 2242
Size: 1014 685 4510
Fragments arranged by size:
4510 1014 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6209
PvuII CAG'CTG
Cuts at: 423 3634 3994 423
Size: 3211 360 2638
Fragments arranged by size:
3211 2638 360
RsrII CG'GwC_CG
Cuts at: 4404 4404
Size: 6209
SacI G_AGCT'C
Cuts at: 924 1278 2308 3223 924
Size: 354 1030 915 3910
Fragments arranged by size:
3910 1030 915 354
SalI G'TCGA_C
Cuts at: 3246 3246
Size: 6209
SanDI GG'GwC_CC
Cuts at: 189 189
Size: 6209
SapI GCTCTTCn'nnn_
Cuts at: 4232 4442 4232
Size: 210 5999
SbfI CC_TGCA'GG
Cuts at: 3256 3256
Size: 6209
ScaI AGT'ACT
Cuts at: 5726 5726
Size: 6209
SfcI C'TryA_G
Cuts at: 33 1220 1502 1776 2238 2402 3252 3937 4930
Size: 1187 282 274 462 164 850 685 993
Cuts at: 4930 5121 33
Size: 191 1121
Fragments arranged by size:
1187 1121 993 850 685 462 282 274
191 164
SfoI GGC'GCC
Cuts at: 3641 3889 3641
Size: 248 5961
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6209
SmaI CCC'GGG
Cuts at: 363 3231 363
Size: 2868 3341
SmlI C'TyrA_G
Cuts at: 2271 2908 3364 4279 4771 5033 2271
Size: 637 456 915 492 262 3447
Fragments arranged by size:
3447 915 637 492 456 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6209
SphI G_CATG'C
Cuts at: 2350 3262 3762 2350
Size: 912 500 4797
Fragments arranged by size:
4797 912 500
StyI C'CwwG_G
Cuts at: 107 1854 3343 3697 107
Size: 1747 1489 354 2619
Fragments arranged by size:
2619 1747 1489 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 261 304 1104 1457 3792 3925 4384 4593 261
Size: 43 800 353 2335 133 459 209 1877
Fragments arranged by size:
2335 1877 800 459 353 209 133 43
TatI w'GTAC_w
Cuts at: 1130 1882 2771 3078 5724 1130
Size: 752 889 307 2646 1615
Fragments arranged by size:
2646 1615 889 752 307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 515 547 5726 5758 515
Size: 32 5179 32 966
Fragments arranged by size:
5179 966 32 32
Tth111I GACn'n_nGTC
Cuts at: 4006 4006
Size: 6209
XbaI T'CTAG_A
Cuts at: 3240 3240
Size: 6209
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2574 2650 2886 3704 2574
Size: 76 236 818 5079
Fragments arranged by size:
5079 818 236 76
XmaI C'CCGG_G
Cuts at: 361 3229 361
Size: 2868 3341
XmnI GAAnn'nnTTC
Cuts at: 1367 1367
Size: 6209
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BbvCI BcgI BglI BmtI BsiWI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FspAI HpaI
MfeI NheI NotI NruI PacI PasI PmlI PshAI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI