Restriction map for
pF1KB7327
(Circular) (Six-base) MAPSORT of: pF1KB7327.seq Check: 9061 from: 1 to: 5627
pF1KB7327 5627 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 286 306 949 1626 2665 286
Size: 20 643 677 1039 3248
Fragments arranged by size:
3248 1039 677 643 20
Acc65I G'GTAC_C
Cuts at: 299 782 2643 299
Size: 483 1861 3283
Fragments arranged by size:
3283 1861 483
AclI AA'CG_TT
Cuts at: 5467 5467
Size: 5627
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 1400 1483 1790 1801 2016 2197 2528 2615
Size: 790 83 307 11 215 181 331 87
Cuts at: 2615 3452 3884 4631 4899 610
Size: 837 432 747 268 1338
Fragments arranged by size:
1338 837 790 747 432 331 307 268
215 181 87 83 11
AflIII A'CryG_T
Cuts at: 1577 2979 4083 5060 1577
Size: 1402 1104 977 2144
Fragments arranged by size:
2144 1402 1104 977
AgeI A'CCGG_T
Cuts at: 4015 4015
Size: 5627
AleI CACnn'nnGTG
Cuts at: 178 178
Size: 5627
AlwNI CAG_nnn'CTG
Cuts at: 2932 4499 2932
Size: 1567 4060
ApaLI G'TGCA_C
Cuts at: 4397 4397
Size: 5627
ApoI r'AATT_y
Cuts at: 293 346 447 545 694 973 1867 1896 2397
Size: 53 101 98 149 279 894 29 501
Cuts at: 2397 2589 2631 4980 5236 293
Size: 192 42 2349 256 684
Fragments arranged by size:
2349 894 684 501 279 256 192 149
101 98 53 42 29
AseI AT'TA_AT
Cuts at: 20 833 2150 2419 20
Size: 813 1317 269 3228
Fragments arranged by size:
3228 1317 813 269
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5627
AvaI C'yCGr_G
Cuts at: 2647 2647
Size: 5627
BamHI G'GATC_C
Cuts at: 1585 2097 2652 1585
Size: 512 555 4560
Fragments arranged by size:
4560 555 512
BanI G'GyrC_C
Cuts at: 299 782 2643 3057 3305 3340 299
Size: 483 1861 414 248 35 2586
Fragments arranged by size:
2586 1861 483 414 248 35
BanII G_rGCy'C
Cuts at: 2641 3671 2641
Size: 1030 4597
BbeI G_GCGC'C
Cuts at: 3061 3309 3061
Size: 248 5379
BbsI GAAGACnn'nnnn_
Cuts at: 1464 1464
Size: 5627
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5593
BciVI GTATCCnnnnn_n'
Cuts at: 3525 4286 5627 3525
Size: 761 1341 3525
Fragments arranged by size:
3525 1341 761
BclI T'GATC_A
Cuts at: 1700 2342 2529 3147 1700
Size: 642 187 618 4180
Fragments arranged by size:
4180 642 618 187
BglII A'GATC_T
Cuts at: 621 1538 2493 3142 5179 621
Size: 917 955 649 2037 1069
Fragments arranged by size:
2037 1069 955 917 649
BlpI GC'TnA_GC
Cuts at: 2739 2739
Size: 5627
Bme1580I G_kGCm'C
Cuts at: 3252 3345 4401 3252
Size: 93 1056 4478
Fragments arranged by size:
4478 1056 93
BmgBI CAC'GTC
Cuts at: 1100 1100
Size: 5627
BmrI ACTGGGnnnn_n'
Cuts at: 703 2196 2839 2920 3255 5399 703
Size: 1493 643 81 335 2144 931
Fragments arranged by size:
2144 1493 931 643 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3994 3994
Size: 5627
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 785 134
Size: 651 4976
Bpu10I CC'TnA_GC
Cuts at: 808 808
Size: 5627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2803 3682 4174 4472 2803
Size: 879 492 298 3958
Fragments arranged by size:
3958 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1115 5331 1115
Size: 4216 1411
BsaAI yAC'GTr
Cuts at: 2980 2980
Size: 5627
BsaBI GATnn'nnATC
Cuts at: 6 2492 3141 5178 6
Size: 2486 649 2037 455
Fragments arranged by size:
2486 2037 649 455
BsaHI Gr'CG_yC
Cuts at: 3058 3306 4008 5504 3058
Size: 248 702 1496 3181
Fragments arranged by size:
3181 1496 702 248
BsaWI w'CCGG_w
Cuts at: 713 2816 3337 4015 4289 4436 4847 713
Size: 2103 521 678 274 147 411 1493
Fragments arranged by size:
2103 1493 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1242 1272 1242
Size: 30 5597
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 585 588 782 926 585
Size: 3 194 144 5286
Fragments arranged by size:
5286 194 144 3
BseYI C'CCAG_C
Cuts at: 3052 3810 4387 4883 3052
Size: 758 577 496 3796
Fragments arranged by size:
3796 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1411 1411
Size: 5627
BsiEI CG_ry'CG
Cuts at: 104 3169 3215 4423 104
Size: 3065 46 1208 1308
Fragments arranged by size:
3065 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2641 3419 3609 4401 2641
Size: 778 190 792 3867
Fragments arranged by size:
3867 792 778 190
BsmI GAATG_Cn'
Cuts at: 1931 2544 1931
Size: 613 5014
Bsp1286I G_dGCh'C
Cuts at: 2641 3252 3345 3419 3609 3671 4401 2641
Size: 611 93 74 190 62 730 3867
Fragments arranged by size:
3867 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 713 4847 713
Size: 4134 1493
BspHI T'CATG_A
Cuts at: 4803 4803
Size: 5627
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2677 2891 3193 3574 2677
Size: 214 302 381 4730
Fragments arranged by size:
4730 381 302 214
BsrBI CCG'CTC
Cuts at: 50 243 3919 3973 5457 5622 50
Size: 193 3676 54 1484 165 55
Fragments arranged by size:
3676 1484 193 165 55 54
BsrDI GCAATG_nn'
Cuts at: 161 881 1140 1291 3539 161
Size: 720 259 151 2248 2249
Fragments arranged by size:
2249 2248 720 259 151
BsrFI r'CCGG_y
Cuts at: 3625 3806 4015 3625
Size: 181 209 5237
Fragments arranged by size:
5237 209 181
BssSI C'ACGA_G
Cuts at: 2129 3898 4256 2129
Size: 1769 358 3500
Fragments arranged by size:
3500 1769 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1425 3004 1425
Size: 1579 4048
BstBI TT'CG_AA
Cuts at: 643 3988 643
Size: 3345 2282
BstYI r'GATC_y
Cuts at: 1 621 1538 1585 2097 2208 2406 2493 2652
Size: 620 917 47 512 111 198 87 159
Cuts at: 2652 3119 3142 3477 3723 4735 4821 4833 5179
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5179 1
Size: 449
Fragments arranged by size:
1012 917 620 512 467 449 346 335
246 198 159 111 87 86 47 23
12
Bsu36I CC'TnA_GG
Cuts at: 2411 2411
Size: 5627
BtgI C'CryG_G
Cuts at: 125 125
Size: 5627
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 792 3577 3758 3921 792
Size: 2785 181 163 2498
Fragments arranged by size:
2785 2498 181 163
BtsI GCAGTG_nn'
Cuts at: 173 2870 173
Size: 2697 2930
ClaI AT'CG_AT
Cuts at: 1877 2291 1877
Size: 414 5213
DraI TTT'AAA
Cuts at: 428 2626 428
Size: 2198 3429
DraIII CAC_nnn'GTG
Cuts at: 1959 1959
Size: 5627
DrdI GACnn_nn'nnGTC
Cuts at: 3333 4191 3333
Size: 858 4769
EaeI y'GGCC_r
Cuts at: 191 3212 3386 3777 3804 5149 191
Size: 3021 174 391 27 1345 669
Fragments arranged by size:
3021 1345 669 391 174 27
EagI C'GGCC_G
Cuts at: 3212 3212
Size: 5627
EarI CTCTTCn'nnn_
Cuts at: 197 461 492 3650 3860 197
Size: 264 31 3158 210 1964
Fragments arranged by size:
3158 1964 264 210 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3835 4145 4291 5437 3835
Size: 310 146 1146 4025
Fragments arranged by size:
4025 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2639 2639
Size: 5627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 610 785 1400 1483 1790 1801 2016 2197
Size: 476 175 615 83 307 11 215 181
Cuts at: 2197 2528 2615 3452 3884 4631 4899 134
Size: 331 87 837 432 747 268 862
Fragments arranged by size:
862 837 747 615 476 432 331 307
268 215 181 175 87 83 11
EcoO109I rG'GnC_Cy
Cuts at: 922 2766 922
Size: 1844 3783
EcoRI G'AATT_C
Cuts at: 973 2631 973
Size: 1658 3969
EcoRV GAT'ATC
Cuts at: 996 996
Size: 5627
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 232 2418 3646 232
Size: 2186 1228 2213
Fragments arranged by size:
2213 2186 1228
FspI TGC'GCA
Cuts at: 3408 3408
Size: 5627
HaeII r_GCGC'y
Cuts at: 3061 3309 4331 5310 3061
Size: 248 1022 979 3378
Fragments arranged by size:
3378 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 517 549 577 609 652 684 1242
Size: 32 217 32 28 32 43 32 558
Cuts at: 1242 1274 1490 1522 1827 1859 1905 1937 2462
Size: 32 216 32 305 32 46 32 525
Cuts at: 2462 2494 3469 3499 3501 3531 268
Size: 32 975 30 2 30 2364
Fragments arranged by size:
2364 975 558 525 305 217 216 46
43 32 32 32 32 32 32 32
32 32 30 30 28 2
HincII GTy'rAC
Cuts at: 287 1627 2666 287
Size: 1340 1039 3248
Fragments arranged by size:
3248 1340 1039
HindIII A'AGCT_T
Cuts at: 1335 1335
Size: 5627
KasI G'GCGC_C
Cuts at: 3057 3305 3057
Size: 248 5379
KpnI G_GTAC'C
Cuts at: 303 786 2647 303
Size: 483 1861 3283
Fragments arranged by size:
3283 1861 483
MfeI C'AATT_G
Cuts at: 452 1568 1604 452
Size: 1116 36 4475
Fragments arranged by size:
4475 1116 36
MluI A'CGCG_T
Cuts at: 5060 5060
Size: 5627
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1970 2186 4298 4482 1970
Size: 216 2112 184 3115
Fragments arranged by size:
3115 2112 216 184
MscI TGG'CCA
Cuts at: 3388 3388
Size: 5627
MslI CAynn'nnrTG
Cuts at: 178 1999 3616 3743 178
Size: 1821 1617 127 2062
Fragments arranged by size:
2062 1821 1617 127
MspA1I CmG'CkG
Cuts at: 115 127 134 2317 2738 3052 3412 4425 4670
Size: 12 7 2183 421 314 360 1013 245
Cuts at: 4670 115
Size: 1072
Fragments arranged by size:
2183 1072 1013 421 360 314 245 12
7
NaeI GCC'GGC
Cuts at: 3808 3808
Size: 5627
NarI GG'CG_CC
Cuts at: 3058 3306 3058
Size: 248 5379
NdeI CA'TA_TG
Cuts at: 1421 1421
Size: 5627
NgoMIV G'CCGG_C
Cuts at: 3806 3806
Size: 5627
NspI r_CATG'y
Cuts at: 270 1581 1989 2082 2680 3180 4087 270
Size: 1311 408 93 598 500 907 1810
Fragments arranged by size:
1810 1311 907 598 500 408 93
PciI A'CATG_T
Cuts at: 1577 4083 1577
Size: 2506 3121
PmeI GTTT'AAAC
Cuts at: 2626 2626
Size: 5627
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4766 4798 4766
Size: 32 5595
PpuMI rG'GwC_Cy
Cuts at: 922 922
Size: 5627
PshAI GACnn'nnGTC
Cuts at: 1464 1464
Size: 5627
PstI C_TGCA'G
Cuts at: 1790 2163 2674 3359 1790
Size: 373 511 685 4058
Fragments arranged by size:
4058 685 511 373
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5627
PvuII CAG'CTG
Cuts at: 2317 3052 3412 2317
Size: 735 360 4532
Fragments arranged by size:
4532 735 360
RsrII CG'GwC_CG
Cuts at: 3822 3822
Size: 5627
SacI G_AGCT'C
Cuts at: 2641 2641
Size: 5627
SacII CC_GC'GG
Cuts at: 128 128
Size: 5627
SalI G'TCGA_C
Cuts at: 285 1625 2664 285
Size: 1340 1039 3248
Fragments arranged by size:
3248 1340 1039
SapI GCTCTTCn'nnn_
Cuts at: 3650 3860 3650
Size: 210 5417
SbfI CC_TGCA'GG
Cuts at: 2674 2674
Size: 5627
ScaI AGT'ACT
Cuts at: 5144 5144
Size: 5627
SfcI C'TryA_G
Cuts at: 33 1760 1786 1847 2159 2670 3355 4348 4539
Size: 1727 26 61 312 511 685 993 191
Cuts at: 4539 33
Size: 1121
Fragments arranged by size:
1727 1121 993 685 511 312 191 61
26
SfoI GGC'GCC
Cuts at: 3059 3307 3059
Size: 248 5379
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5627
SmaI CCC'GGG
Cuts at: 2649 2649
Size: 5627
SmlI C'TyrA_G
Cuts at: 2782 3697 4189 4451 2782
Size: 915 492 262 3958
Fragments arranged by size:
3958 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1360 67
Size: 1293 4334
SphI G_CATG'C
Cuts at: 2680 3180 2680
Size: 500 5127
SspI AAT'ATT
Cuts at: 519 1053 519
Size: 534 5093
StyI C'CwwG_G
Cuts at: 2761 3115 2761
Size: 354 5273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1139 1742 3210 3343 3802 4011 1139
Size: 603 1468 133 459 209 2755
Fragments arranged by size:
2755 1468 603 459 209 133
TatI w'GTAC_w
Cuts at: 2125 5142 2125
Size: 3017 2610
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 980 1012 5144 5176 980
Size: 32 4132 32 1431
Fragments arranged by size:
4132 1431 32 32
Tth111I GACn'n_nGTC
Cuts at: 709 3424 709
Size: 2715 2912
XbaI T'CTAG_A
Cuts at: 2437 2496 2658 2437
Size: 59 162 5406
Fragments arranged by size:
5406 162 59
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3122 3122
Size: 5627
XmaI C'CCGG_G
Cuts at: 2647 2647
Size: 5627
XmnI GAAnn'nnTTC
Cuts at: 623 623
Size: 5627
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I CspCI EcoNI
FseI FspAI HpaI NcoI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI