Restriction map for
pF1KB7323
(Circular) (Six-base) MAPSORT of: pF1KB7323.seq Check: 4499 from: 1 to: 8975
pF1KB7323 8975 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 792 1195 1433 3403 3576 792
Size: 403 238 1970 173 6191
Fragments arranged by size:
6191 1970 403 238 173
AccI GT'mk_AC
Cuts at: 6013 6013
Size: 8975
Acc65I G'GTAC_C
Cuts at: 5991 5991
Size: 8975
AclI AA'CG_TT
Cuts at: 8815 8815
Size: 8975
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 1522 2728 3158 3226 3483 4327 5618 6800
Size: 940 1206 430 68 257 844 1291 1182
Cuts at: 6800 7232 7979 8247 582
Size: 432 747 268 1310
Fragments arranged by size:
1310 1291 1206 1182 940 844 747 432
430 268 257 68
AfeI AGC'GCT
Cuts at: 1849 1849
Size: 8975
AflIII A'CryG_T
Cuts at: 2912 6327 7431 8408 2912
Size: 3415 1104 977 3479
Fragments arranged by size:
3479 3415 1104 977
AgeI A'CCGG_T
Cuts at: 7363 7363
Size: 8975
AhdI GACnn_n'nnGTC
Cuts at: 180 180
Size: 8975
AleI CACnn'nnGTG
Cuts at: 3586 4532 4756 3586
Size: 946 224 7805
Fragments arranged by size:
7805 946 224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2320 3622 2320
Size: 1302 7673
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 538 570 2476 2508 2659 2691 2749 2781 538
Size: 32 1906 32 151 32 58 32 6732
Fragments arranged by size:
6732 1906 151 58 32 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3672 4875 5631 6280 7847 3672
Size: 1203 756 649 1567 4800
Fragments arranged by size:
4800 1567 1203 756 649
ApaI G_GGCC'C
Cuts at: 118 2256 2360 3381 4709 5894 5908 5954 118
Size: 2138 104 1021 1328 1185 14 46 3139
Fragments arranged by size:
3139 2138 1328 1185 1021 104 46 14
ApaLI G'TGCA_C
Cuts at: 1930 1971 7745 1930
Size: 41 5774 3160
Fragments arranged by size:
5774 3160 41
ApoI r'AATT_y
Cuts at: 2560 5206 5979 8328 8584 2560
Size: 2646 773 2349 256 2951
Fragments arranged by size:
2951 2646 2349 773 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8975
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8975
AvaI C'yCGr_G
Cuts at: 507 975 1191 1240 1450 3373 3407 3557 4346
Size: 468 216 49 210 1923 34 150 789
Cuts at: 4346 4580 4708 4849 4958 5995 507
Size: 234 128 141 109 1037 3487
Fragments arranged by size:
3487 1923 1037 789 468 234 216 210
150 141 128 109 49 34
BamHI G'GATC_C
Cuts at: 1280 6000 1280
Size: 4720 4255
BanI G'GyrC_C
Cuts at: 124 673 804 2421 3227 4082 4526 5315 5321
Size: 549 131 1617 806 855 444 789 6
Cuts at: 5321 5991 6405 6653 6688 124
Size: 670 414 248 35 2411
Fragments arranged by size:
2411 1617 855 806 789 670 549 444
414 248 131 35 6
BanII G_rGCy'C
Cuts at: 118 239 500 929 1547 1592 2256 2360 2591
Size: 121 261 429 618 45 664 104 231
Cuts at: 2591 2620 2945 3381 3967 4202 4709 4939 4959
Size: 29 325 436 586 235 507 230 20
Cuts at: 4959 5138 5420 5894 5908 5954 5989 7019 118
Size: 179 282 474 14 46 35 1030 2074
Fragments arranged by size:
2074 1030 664 618 586 507 474 436
429 325 282 261 235 231 230 179
121 104 46 45 35 29 20 14
BbeI G_GCGC'C
Cuts at: 2425 5325 6409 6657 2425
Size: 2900 1084 248 4743
Fragments arranged by size:
4743 2900 1084 248
BbsI GAAGACnn'nnnn_
Cuts at: 1796 2066 2855 3164 1796
Size: 270 789 309 7607
Fragments arranged by size:
7607 789 309 270
BbvCI CC'TCA_GC
Cuts at: 2104 5123 2104
Size: 3019 5956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1036 1070 90
Size: 34 912 34 7995
Fragments arranged by size:
7995 912 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5298 6873 7634 8975 5298
Size: 1575 761 1341 5298
Fragments arranged by size:
5298 1575 1341 761
BclI T'GATC_A
Cuts at: 1940 6495 1940
Size: 4555 4420
BglI GCCn_nnn'nGGC
Cuts at: 122 2079 3121 3376 4272 5593 5949 122
Size: 1957 1042 255 896 1321 356 3148
Fragments arranged by size:
3148 1957 1321 1042 896 356 255
BglII A'GATC_T
Cuts at: 6490 8527 6490
Size: 2037 6938
BlpI GC'TnA_GC
Cuts at: 2962 5065 5710 6087 2962
Size: 2103 645 377 5850
Fragments arranged by size:
5850 2103 645 377
Bme1580I G_kGCm'C
Cuts at: 118 127 809 1934 1975 2256 2263 2360 3230
Size: 9 682 1125 41 281 7 97 870
Cuts at: 3230 3381 4580 4709 4763 4857 5894 5908 5954
Size: 151 1199 129 54 94 1037 14 46
Cuts at: 5954 6600 6693 7749 118
Size: 646 93 1056 1344
Fragments arranged by size:
1344 1199 1125 1056 1037 870 682 646
281 151 129 97 94 93 54 46
41 14 9 7
BmgBI CAC'GTC
Cuts at: 543 1584 2913 3720 3852 5730 543
Size: 1041 1329 807 132 1878 3788
Fragments arranged by size:
3788 1878 1329 1041 807 132
BmrI ACTGGGnnnn_n'
Cuts at: 112 2040 2406 4074 6187 6268 6603 8747 112
Size: 1928 366 1668 2113 81 335 2144 340
Fragments arranged by size:
2144 2113 1928 1668 366 340 335 81
BmtI G_CTAG'C
Cuts at: 787 787
Size: 8975
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2364 2484 4608 4752 7342 2364
Size: 120 2124 144 2590 3997
Fragments arranged by size:
3997 2590 2124 144 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 878 1249 1551 1636 1720 2242 2774 2776 2830
Size: 371 302 85 84 522 532 2 54
Cuts at: 2830 2842 3295 3307 4578 5080 5473 5515 5719
Size: 12 453 12 1271 502 393 42 204
Cuts at: 5719 878
Size: 4134
Fragments arranged by size:
4134 1271 532 522 502 453 393 371
302 204 85 84 54 42 12 12
2
Bpu10I CC'TnA_GC
Cuts at: 1607 2104 2893 3961 5123 5806 1607
Size: 497 789 1068 1162 683 4776
Fragments arranged by size:
4776 1162 1068 789 683 497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 400 913 2002 2071 2647 2887 3670 6151
Size: 141 513 1089 69 576 240 783 2481
Cuts at: 6151 7030 7522 7820 259
Size: 879 492 298 1414
Fragments arranged by size:
2481 1414 1089 879 783 576 513 492
298 240 141 69
BsaI GGTCTCn'nnnn_
Cuts at: 4552 8679 4552
Size: 4127 4848
BsaAI yAC'GTr
Cuts at: 2436 2568 3987 4605 6328 2436
Size: 132 1419 618 1723 5083
Fragments arranged by size:
5083 1723 1419 618 132
BsaBI GATnn'nnATC
Cuts at: 6 6489 8526 6
Size: 6483 2037 455
Fragments arranged by size:
6483 2037 455
BsaHI Gr'CG_yC
Cuts at: 1403 2422 4442 5322 6406 6654 7356 8852 1403
Size: 1019 2020 880 1084 248 702 1496 1526
Fragments arranged by size:
2020 1526 1496 1084 1019 880 702 248
BsaWI w'CCGG_w
Cuts at: 3908 5525 6164 6685 7363 7637 7784 8195 3908
Size: 1617 639 521 678 274 147 411 4688
Fragments arranged by size:
4688 1617 678 639 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 487 517 1018 1048 2173 2203 2473 2503 2749
Size: 30 501 30 1125 30 270 30 246
Cuts at: 2749 2779 3949 3979 4153 4183 5488 5518 5909
Size: 30 1170 30 174 30 1305 30 391
Cuts at: 5909 5939 487
Size: 30 3523
Fragments arranged by size:
3523 1305 1170 1125 501 391 270 246
174 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 148 172 577 963 1012 1095 1330 1333
Size: 3 24 405 386 49 83 235 3
Cuts at: 1333 1348 1351 1354 1404 1858 2764 2773 2839
Size: 15 3 3 50 454 906 9 66
Cuts at: 2839 3388 3586 4012 4027 4057 4728 4978 4990
Size: 549 198 426 15 30 671 250 12
Cuts at: 4990 5035 5140 5287 5530 5716 5853 145
Size: 45 105 147 243 186 137 3267
Fragments arranged by size:
3267 906 671 549 454 426 405 386
250 243 235 198 186 147 137 105
83 66 50 49 45 30 24 15
15 12 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 809 1591 2052 2877 3380 4371 4421 4794 4820
Size: 782 461 825 503 991 50 373 26
Cuts at: 4820 4824 4938 5650 6400 7158 7735 8231 809
Size: 4 114 712 750 758 577 496 1553
Fragments arranged by size:
1553 991 825 782 758 750 712 577
503 496 461 373 114 50 26 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2191 2326 2572 4574 5662 2191
Size: 135 246 2002 1088 5504
Fragments arranged by size:
5504 2002 1088 246 135
BsiEI CG_ry'CG
Cuts at: 104 923 6517 6563 7771 104
Size: 819 5594 46 1208 1308
Fragments arranged by size:
5594 1308 1208 819 46
BsiHKAI G_wGCw'C
Cuts at: 1934 1975 2591 2945 3239 3967 4202 5989 6767
Size: 41 616 354 294 728 235 1787 778
Cuts at: 6767 6957 7749 1934
Size: 190 792 3160
Fragments arranged by size:
3160 1787 792 778 728 616 354 294
235 190 41
BsmI GAATG_Cn'
Cuts at: 1054 1054
Size: 8975
BsmBI CGTCTCn'nnnn_
Cuts at: 754 4972 754
Size: 4218 4757
Bsp1286I G_dGCh'C
Cuts at: 118 127 239 500 809 929 1547 1592 1934
Size: 9 112 261 309 120 618 45 342
Cuts at: 1934 1975 2256 2263 2360 2591 2620 2945 3230
Size: 41 281 7 97 231 29 325 285
Cuts at: 3230 3239 3381 3967 4202 4580 4709 4763 4857
Size: 9 142 586 235 378 129 54 94
Cuts at: 4857 4939 4959 5138 5420 5894 5908 5954 5989
Size: 82 20 179 282 474 14 46 35
Cuts at: 5989 6600 6693 6767 6957 7019 7749 118
Size: 611 93 74 190 62 730 1344
Fragments arranged by size:
1344 730 618 611 586 474 378 342
325 309 285 282 281 261 235 231
190 179 142 129 120 112 97 94
93 82 74 62 54 46 45 41
35 29 20 14 9 9 7
BspEI T'CCGG_A
Cuts at: 5525 8195 5525
Size: 2670 6305
BspHI T'CATG_A
Cuts at: 3062 8151 3062
Size: 5089 3886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 792 1195 1433 1964 2882 3403 3576 4140 5471
Size: 403 238 531 918 521 173 564 1331
Cuts at: 5471 6025 6239 6541 6922 792
Size: 554 214 302 381 2845
Fragments arranged by size:
2845 1331 918 564 554 531 521 403
381 302 238 214 173
BsrBI CCG'CTC
Cuts at: 50 2468 3312 4023 4968 5277 7267 7321 8805
Size: 2418 844 711 945 309 1990 54 1484
Cuts at: 8805 8970 50
Size: 165 55
Fragments arranged by size:
2418 1990 1484 945 844 711 309 165
55 54
BsrDI GCAATG_nn'
Cuts at: 327 620 1428 1671 6887 327
Size: 293 808 243 5216 2415
Fragments arranged by size:
5216 2415 808 293 243
BsrFI r'CCGG_y
Cuts at: 2928 3750 5318 6973 7154 7363 2928
Size: 822 1568 1655 181 209 4540
Fragments arranged by size:
4540 1655 1568 822 209 181
BsrGI T'GTAC_A
Cuts at: 5873 5873
Size: 8975
BssHII G'CGCG_C
Cuts at: 2372 2372
Size: 8975
BssSI C'ACGA_G
Cuts at: 4930 7246 7604 4930
Size: 2316 358 6301
Fragments arranged by size:
6301 2316 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2874 3234 4605 6352 2874
Size: 360 1371 1747 5497
Fragments arranged by size:
5497 1747 1371 360
BstBI TT'CG_AA
Cuts at: 7336 7336
Size: 8975
BstEII G'GTnAC_C
Cuts at: 765 2439 765
Size: 1674 7301
BstXI CCAn_nnnn'nTGG
Cuts at: 1162 3264 3903 1162
Size: 2102 639 6234
Fragments arranged by size:
6234 2102 639
BstYI r'GATC_y
Cuts at: 1 348 641 1280 1454 5045 6000 6467 6490
Size: 347 293 639 174 3591 955 467 23
Cuts at: 6490 6825 7071 8083 8169 8181 8527 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
3591 1012 955 639 467 449 347 346
335 293 246 174 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 610 4700 610
Size: 4090 4885
BtgI C'CryG_G
Cuts at: 142 722 2153 2176 3116 3437 5395 5573 142
Size: 580 1431 23 940 321 1958 178 3544
Fragments arranged by size:
3544 1958 1431 940 580 321 178 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5211 6925 7106 7269 5211
Size: 1714 181 163 6917
Fragments arranged by size:
6917 1714 181 163
BtsI GCAGTG_nn'
Cuts at: 384 2203 3870 6218 384
Size: 1819 1667 2348 3141
Fragments arranged by size:
3141 2348 1819 1667
ClaI AT'CG_AT
Cuts at: 1985 1985
Size: 8975
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1411 1446 1411
Size: 35 8940
DraI TTT'AAA
Cuts at: 5974 5974
Size: 8975
DraIII CAC_nnn'GTG
Cuts at: 1716 3234 3621 5261 1716
Size: 1518 387 1640 5430
Fragments arranged by size:
5430 1640 1518 387
DrdI GACnn_nn'nnGTC
Cuts at: 2155 6681 7539 2155
Size: 4526 858 3591
Fragments arranged by size:
4526 3591 858
EaeI y'GGCC_r
Cuts at: 521 920 2276 2930 3434 3519 3590 3797 3887
Size: 399 1356 654 504 85 71 207 90
Cuts at: 3887 4969 5771 6560 6734 7125 7152 8497 521
Size: 1082 802 789 174 391 27 1345 999
Fragments arranged by size:
1356 1345 1082 999 802 789 654 504
399 391 207 174 90 85 71 27
EagI C'GGCC_G
Cuts at: 920 6560 920
Size: 5640 3335
EarI CTCTTCn'nnn_
Cuts at: 338 350 1066 1090 1307 1801 3106 3712 4253
Size: 12 716 24 217 494 1305 606 541
Cuts at: 4253 4303 4477 4522 6998 7208 338
Size: 50 174 45 2476 210 2105
Fragments arranged by size:
2476 2105 1305 716 606 541 494 217
210 174 50 45 24 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3457 4561 7183 7493 7639 8785 3457
Size: 1104 2622 310 146 1146 3647
Fragments arranged by size:
3647 2622 1146 1104 310 146
EcoICRI GAG'CTC
Cuts at: 2589 2943 3965 4200 5987 2589
Size: 354 1022 235 1787 5577
Fragments arranged by size:
5577 1787 1022 354 235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 878 1249 1522 1551 1636 1720 2242 2728
Size: 296 371 273 29 85 84 522 486
Cuts at: 2728 2774 2776 2830 2842 3158 3226 3295 3307
Size: 46 2 54 12 316 68 69 12
Cuts at: 3307 3483 4327 4578 5080 5473 5515 5618 5719
Size: 176 844 251 502 393 42 103 101
Cuts at: 5719 6800 7232 7979 8247 582
Size: 1081 432 747 268 1310
Fragments arranged by size:
1310 1081 844 747 522 502 486 432
393 371 316 296 273 268 251 176
103 101 85 84 69 68 54 46
42 29 12 12 2
EcoNI CCTnn'n_nnAGG
Cuts at: 1191 2020 2335 3274 3343 4126 5635 5680 1191
Size: 829 315 939 69 783 1509 45 4486
Fragments arranged by size:
4486 1509 939 829 783 315 69 45
EcoO109I rG'GnC_Cy
Cuts at: 114 531 853 1196 1576 1821 2226 2356 2357
Size: 417 322 343 380 245 405 130 1
Cuts at: 2357 2735 3123 3336 3377 4107 4470 4587 4705
Size: 378 388 213 41 730 363 117 118
Cuts at: 4705 4887 4989 5556 5685 5832 5904 5905 5950
Size: 182 102 567 129 147 72 1 45
Cuts at: 5950 5951 6114 114
Size: 1 163 2975
Fragments arranged by size:
2975 730 567 417 405 388 380 378
363 343 322 245 213 182 163 147
130 129 118 117 102 72 45 41
1 1 1
EcoRI G'AATT_C
Cuts at: 5979 5979
Size: 8975
EcoRV GAT'ATC
Cuts at: 5967 5967
Size: 8975
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 954 6994 954
Size: 6040 2935
FspI TGC'GCA
Cuts at: 6756 6756
Size: 8975
HaeII r_GCGC'y
Cuts at: 698 1019 1851 2425 5325 5855 6409 6657 7679
Size: 321 832 574 2900 530 554 248 1022
Cuts at: 7679 8658 698
Size: 979 1015
Fragments arranged by size:
2900 1022 1015 979 832 574 554 530
321 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 166 198 316 348 487 519 1762 1794 3142
Size: 32 118 32 139 32 1243 32 1348
Cuts at: 3142 3174 3760 3792 4186 4198 4218 4230 5909
Size: 32 586 32 394 12 20 12 1679
Cuts at: 5909 5941 6817 6847 6849 6879 166
Size: 32 876 30 2 30 2262
Fragments arranged by size:
2262 1679 1348 1243 876 586 394 139
118 32 32 32 32 32 32 32
30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 4155 4668 5928 6014 4155
Size: 513 1260 86 7116
Fragments arranged by size:
7116 1260 513 86
KasI G'GCGC_C
Cuts at: 2421 5321 6405 6653 2421
Size: 2900 1084 248 4743
Fragments arranged by size:
4743 2900 1084 248
KpnI G_GTAC'C
Cuts at: 5995 5995
Size: 8975
MluI A'CGCG_T
Cuts at: 8408 8408
Size: 8975
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 344 983 5771 7646 7830 344
Size: 639 4788 1875 184 1489
Fragments arranged by size:
4788 1875 1489 639 184
MscI TGG'CCA
Cuts at: 3436 3592 6736 3436
Size: 156 3144 5675
Fragments arranged by size:
5675 3144 156
MslI CAynn'nnrTG
Cuts at: 1717 3064 3586 4088 4366 4532 4756 6964 7091
Size: 1347 522 502 278 166 224 2208 127
Cuts at: 7091 1717
Size: 3601
Fragments arranged by size:
3601 2208 1347 522 502 278 224 166
127
MspA1I CmG'CkG
Cuts at: 144 724 733 1446 1518 1698 2220 4305 4392
Size: 580 9 713 72 180 522 2085 87
Cuts at: 4392 4794 4824 5010 5372 5697 6086 6400 6760
Size: 402 30 186 362 325 389 314 360
Cuts at: 6760 7773 8018 144
Size: 1013 245 1101
Fragments arranged by size:
2085 1101 1013 713 580 522 402 389
362 360 325 314 245 186 180 87
72 30 9
NaeI GCC'GGC
Cuts at: 2930 7156 2930
Size: 4226 4749
NarI GG'CG_CC
Cuts at: 2422 5322 6406 6654 2422
Size: 2900 1084 248 4743
Fragments arranged by size:
4743 2900 1084 248
NcoI C'CATG_G
Cuts at: 2176 5573 2176
Size: 3397 5578
NgoMIV G'CCGG_C
Cuts at: 2928 7154 2928
Size: 4226 4749
NheI G'CTAG_C
Cuts at: 783 783
Size: 8975
NspI r_CATG'y
Cuts at: 2892 2928 4431 4666 6028 6528 7435 2892
Size: 36 1503 235 1362 500 907 4432
Fragments arranged by size:
4432 1503 1362 907 500 235 36
PasI CC'CwG_GG
Cuts at: 1062 2256 5946 1062
Size: 1194 3690 4091
Fragments arranged by size:
4091 3690 1194
PciI A'CATG_T
Cuts at: 7431 7431
Size: 8975
PflMI CCAn_nnn'nTGG
Cuts at: 2627 3621 4461 2627
Size: 994 840 7141
Fragments arranged by size:
7141 994 840
PfoI T'CCnGG_A
Cuts at: 2819 3272 3284 4294 5048 2819
Size: 453 12 1010 754 6746
Fragments arranged by size:
6746 1010 754 453 12
PmeI GTTT'AAAC
Cuts at: 5974 5974
Size: 8975
PmlI CAC'GTG
Cuts at: 2568 4605 2568
Size: 2037 6938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1621 1653 2749 2781 8114 8146 1621
Size: 32 1096 32 5333 32 2450
Fragments arranged by size:
5333 2450 1096 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1821 2735 4107 4470 5556 5685 5832 1821
Size: 914 1372 363 1086 129 147 4964
Fragments arranged by size:
4964 1372 1086 914 363 147 129
PspOMI G'GGCC_C
Cuts at: 114 2252 2356 3377 4705 5890 5904 5950 114
Size: 2138 104 1021 1328 1185 14 46 3139
Fragments arranged by size:
3139 2138 1328 1185 1021 104 46 14
PspXI vC'TCGA_Gb
Cuts at: 975 975
Size: 8975
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2974 3006 2974
Size: 32 8943
PstI C_TGCA'G
Cuts at: 1059 5786 6022 6707 1059
Size: 4727 236 685 3327
Fragments arranged by size:
4727 3327 685 236
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8975
PvuII CAG'CTG
Cuts at: 1698 2220 4305 4824 5010 5697 6400 6760 1698
Size: 522 2085 519 186 687 703 360 3913
Fragments arranged by size:
3913 2085 703 687 522 519 360 186
RsrII CG'GwC_CG
Cuts at: 7170 7170
Size: 8975
SacI G_AGCT'C
Cuts at: 2591 2945 3967 4202 5989 2591
Size: 354 1022 235 1787 5577
Fragments arranged by size:
5577 1787 1022 354 235
SacII CC_GC'GG
Cuts at: 145 725 145
Size: 580 8395
SalI G'TCGA_C
Cuts at: 6012 6012
Size: 8975
SanDI GG'GwC_CC
Cuts at: 2735 5832 2735
Size: 3097 5878
SapI GCTCTTCn'nnn_
Cuts at: 1066 4477 6998 7208 1066
Size: 3411 2521 210 2833
Fragments arranged by size:
3411 2833 2521 210
SbfI CC_TGCA'GG
Cuts at: 6022 6022
Size: 8975
ScaI AGT'ACT
Cuts at: 2983 8492 2983
Size: 5509 3466
SexAI A'CCwGG_T
Cuts at: 2363 3617 2363
Size: 1254 7721
SfcI C'TryA_G
Cuts at: 33 755 1055 1693 3193 3923 3940 5782 6018
Size: 722 300 638 1500 730 17 1842 236
Cuts at: 6018 6703 7696 7887 33
Size: 685 993 191 1121
Fragments arranged by size:
1842 1500 1121 993 730 722 685 638
300 236 191 17
SfoI GGC'GCC
Cuts at: 2423 5323 6407 6655 2423
Size: 2900 1084 248 4743
Fragments arranged by size:
4743 2900 1084 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8975
SgrAI Cr'CCGG_yG
Cuts at: 5318 5318
Size: 8975
SmaI CCC'GGG
Cuts at: 3375 3409 4582 4851 5997 3375
Size: 34 1173 269 1146 6353
Fragments arranged by size:
6353 1173 1146 269 34
SmlI C'TyrA_G
Cuts at: 274 415 928 975 2017 2086 2662 2902 3685
Size: 141 513 47 1042 69 576 240 783
Cuts at: 3685 6130 7045 7537 7799 274
Size: 2445 915 492 262 1450
Fragments arranged by size:
2445 1450 1042 915 783 576 513 492
262 240 141 69 47
SpeI A'CTAG_T
Cuts at: 67 4632 67
Size: 4565 4410
SphI G_CATG'C
Cuts at: 4431 6028 6528 4431
Size: 1597 500 6878
Fragments arranged by size:
6878 1597 500
SspI AAT'ATT
Cuts at: 4222 4222
Size: 8975
StuI AGG'CCT
Cuts at: 595 2026 3496 4399 595
Size: 1431 1470 903 5171
Fragments arranged by size:
5171 1470 1431 903
StyI C'CwwG_G
Cuts at: 839 2176 2843 3026 4864 4901 5117 5573 5636
Size: 1337 667 183 1838 37 216 456 63
Cuts at: 5636 6109 6463 839
Size: 473 354 3351
Fragments arranged by size:
3351 1838 1337 667 473 456 354 216
183 63 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 661 1671 2953 3628 3774 5977 6558 6691 7150
Size: 1010 1282 675 146 2203 581 133 459
Cuts at: 7150 7359 661
Size: 209 2277
Fragments arranged by size:
2277 2203 1282 1010 675 581 459 209
146 133
TatI w'GTAC_w
Cuts at: 1486 2981 4676 5873 8490 1486
Size: 1495 1695 1197 2617 1971
Fragments arranged by size:
2617 1971 1695 1495 1197
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 271 303 1018 1050 3306 3338 4171 4203 8492
Size: 32 715 32 2256 32 833 32 4289
Cuts at: 8492 8524 271
Size: 32 722
Fragments arranged by size:
4289 2256 833 722 715 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3448 6772 3448
Size: 3324 5651
XbaI T'CTAG_A
Cuts at: 6006 6006
Size: 8975
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1466 3669 6470 1466
Size: 2203 2801 3971
Fragments arranged by size:
3971 2801 2203
XhoI C'TCGA_G
Cuts at: 975 975
Size: 8975
XmaI C'CCGG_G
Cuts at: 3373 3407 4580 4849 5995 3373
Size: 34 1173 269 1146 6353
Fragments arranged by size:
6353 1173 1146 269 34
XmnI GAAnn'nnTTC
Cuts at: 2530 5467 2530
Size: 2937 6038
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BaeI BfrBI BsiWI BstZ17I
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PshAI PsiI SfiI SnaBI SrfI SwaI
ZraI