Restriction map for
pF1KB7320
(Circular) (Six-base) MAPSORT of: pF1KB7320.seq Check: 7881 from: 1 to: 6977
pF1KB7320 6977 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 1398 3599 4015 1398
Size: 2201 416 4360
Fragments arranged by size:
4360 2201 416
Acc65I G'GTAC_C
Cuts at: 3993 3993
Size: 6977
AclI AA'CG_TT
Cuts at: 2693 6817 2693
Size: 4124 2853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1513 2444 3641 4802 5234 5981 6249 1513
Size: 931 1197 1161 432 747 268 2241
Fragments arranged by size:
2241 1197 1161 931 747 432 268
AfeI AGC'GCT
Cuts at: 427 427
Size: 6977
AflIII A'CryG_T
Cuts at: 4329 5433 6410 4329
Size: 1104 977 4896
Fragments arranged by size:
4896 1104 977
AgeI A'CCGG_T
Cuts at: 5365 5365
Size: 6977
AleI CACnn'nnGTG
Cuts at: 704 704
Size: 6977
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 370 402 370
Size: 32 6945
AlwNI CAG_nnn'CTG
Cuts at: 250 1699 2659 4282 5849 250
Size: 1449 960 1623 1567 1378
Fragments arranged by size:
1623 1567 1449 1378 960
ApaLI G'TGCA_C
Cuts at: 5747 5747
Size: 6977
ApoI r'AATT_y
Cuts at: 404 1027 1136 1249 1273 1387 2156 2216 2566
Size: 623 109 113 24 114 769 60 350
Cuts at: 2566 3508 3899 3981 6330 6586 404
Size: 942 391 82 2349 256 795
Fragments arranged by size:
2349 942 795 769 623 391 350 256
114 113 109 82 60 24
AseI AT'TA_AT
Cuts at: 20 541 1160 1424 20
Size: 521 619 264 5573
Fragments arranged by size:
5573 619 521 264
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6977
AvaI C'yCGr_G
Cuts at: 1453 3589 3997 1453
Size: 2136 408 4433
Fragments arranged by size:
4433 2136 408
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2240 2273 2240
Size: 33 6944
BamHI G'GATC_C
Cuts at: 3609 4002 3609
Size: 393 6584
BanI G'GyrC_C
Cuts at: 121 181 3379 3993 4407 4655 4690 121
Size: 60 3198 614 414 248 35 2408
Fragments arranged by size:
3198 2408 614 414 248 60 35
BanII G_rGCy'C
Cuts at: 232 345 1873 3991 5021 232
Size: 113 1528 2118 1030 2188
Fragments arranged by size:
2188 2118 1528 1030 113
BbeI G_GCGC'C
Cuts at: 185 4411 4659 185
Size: 4226 248 2503
Fragments arranged by size:
4226 2503 248
BbvCI CC'TCA_GC
Cuts at: 2753 2753
Size: 6977
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6943
BciVI GTATCCnnnnn_n'
Cuts at: 672 4875 5636 6977 672
Size: 4203 761 1341 672
Fragments arranged by size:
4203 1341 761 672
BclI T'GATC_A
Cuts at: 4497 4497
Size: 6977
BglII A'GATC_T
Cuts at: 520 3483 3668 4492 6529 520
Size: 2963 185 824 2037 968
Fragments arranged by size:
2963 2037 968 824 185
BlpI GC'TnA_GC
Cuts at: 4089 4089
Size: 6977
Bme1580I G_kGCm'C
Cuts at: 4602 4695 5751 4602
Size: 93 1056 5828
Fragments arranged by size:
5828 1056 93
BmgBI CAC'GTC
Cuts at: 1998 1998
Size: 6977
BmrI ACTGGGnnnn_n'
Cuts at: 1608 4189 4270 4605 6749 1608
Size: 2581 81 335 2144 1836
Fragments arranged by size:
2581 2144 1836 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5344 5344
Size: 6977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 359 1369 302
Size: 57 1010 5910
Fragments arranged by size:
5910 1010 57
Bpu10I CC'TnA_GC
Cuts at: 203 1676 2753 203
Size: 1473 1077 4427
Fragments arranged by size:
4427 1473 1077
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2788 3022 4153 5032 5524 5822 2788
Size: 234 1131 879 492 298 3943
Fragments arranged by size:
3943 1131 879 492 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 6681 6681
Size: 6977
BsaAI yAC'GTr
Cuts at: 4330 4330
Size: 6977
BsaBI GATnn'nnATC
Cuts at: 6 1631 4491 6528 6
Size: 1625 2860 2037 455
Fragments arranged by size:
2860 2037 1625 455
BsaHI Gr'CG_yC
Cuts at: 182 3581 4408 4656 5358 6854 182
Size: 3399 827 248 702 1496 305
Fragments arranged by size:
3399 1496 827 702 305 248
BsaWI w'CCGG_w
Cuts at: 4166 4687 5365 5639 5786 6197 4166
Size: 521 678 274 147 411 4946
Fragments arranged by size:
4946 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 296 335 965 1273 2168 3701 296
Size: 39 630 308 895 1533 3572
Fragments arranged by size:
3572 1533 895 630 308 39
BseYI C'CCAG_C
Cuts at: 125 231 394 4402 5160 5737 6233 125
Size: 106 163 4008 758 577 496 869
Fragments arranged by size:
4008 869 758 577 496 163 106
BsiEI CG_ry'CG
Cuts at: 104 219 4519 4565 5773 104
Size: 115 4300 46 1208 1308
Fragments arranged by size:
4300 1308 1208 115 46
BsiHKAI G_wGCw'C
Cuts at: 345 510 1873 3991 4769 4959 5751 345
Size: 165 1363 2118 778 190 792 1571
Fragments arranged by size:
2118 1571 1363 792 778 190 165
BsmI GAATG_Cn'
Cuts at: 299 299
Size: 6977
Bsp1286I G_dGCh'C
Cuts at: 232 345 510 1873 3991 4602 4695 4769 4959
Size: 113 165 1363 2118 611 93 74 190
Cuts at: 4959 5021 5751 232
Size: 62 730 1458
Fragments arranged by size:
2118 1458 1363 730 611 190 165 113
93 74 62
BspEI T'CCGG_A
Cuts at: 6197 6197
Size: 6977
BspHI T'CATG_A
Cuts at: 1140 1836 3357 6153 1140
Size: 696 1521 2796 1964
Fragments arranged by size:
2796 1964 1521 696
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1410 3508 4027 4241 4543 4924 1410
Size: 2098 519 214 302 381 3463
Fragments arranged by size:
3463 2098 519 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5269 5323 6807 6972 50
Size: 5219 54 1484 165 55
Fragments arranged by size:
5219 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 329 1886 2483 3179 4889 329
Size: 1557 597 696 1710 2417
Fragments arranged by size:
2417 1710 1557 696 597
BsrFI r'CCGG_y
Cuts at: 4975 5156 5365 4975
Size: 181 209 6587
Fragments arranged by size:
6587 209 181
BsrGI T'GTAC_A
Cuts at: 580 580
Size: 6977
BssSI C'ACGA_G
Cuts at: 1329 5248 5606 1329
Size: 3919 358 2700
Fragments arranged by size:
3919 2700 358
BstAPI GCAn_nnn'nTGC
Cuts at: 250 2679 4354 250
Size: 2429 1675 2873
Fragments arranged by size:
2873 2429 1675
BstBI TT'CG_AA
Cuts at: 747 3861 5338 747
Size: 3114 1477 2386
Fragments arranged by size:
3114 2386 1477
BstXI CCAn_nnnn'nTGG
Cuts at: 1347 1347
Size: 6977
BstYI r'GATC_y
Cuts at: 1 520 3062 3291 3483 3609 3668 4002 4469
Size: 519 2542 229 192 126 59 334 467
Cuts at: 4469 4492 4827 5073 6085 6171 6183 6529 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
2542 1012 519 467 449 346 335 334
246 229 192 126 86 59 23 12
BstZ17I GTA'TAC
Cuts at: 1399 1399
Size: 6977
BtgI C'CryG_G
Cuts at: 753 753
Size: 6977
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3061 3369 4927 5108 5271 3061
Size: 308 1558 181 163 4767
Fragments arranged by size:
4767 1558 308 181 163
BtsI GCAGTG_nn'
Cuts at: 134 462 1661 3235 4220 134
Size: 328 1199 1574 985 2891
Fragments arranged by size:
2891 1574 1199 985 328
DraI TTT'AAA
Cuts at: 1392 1824 3789 3976 1392
Size: 432 1965 187 4393
Fragments arranged by size:
4393 1965 432 187
DrdI GACnn_nn'nnGTC
Cuts at: 4683 5541 4683
Size: 858 6119
EaeI y'GGCC_r
Cuts at: 216 4562 4736 5127 5154 6499 216
Size: 4346 174 391 27 1345 694
Fragments arranged by size:
4346 1345 694 391 174 27
EagI C'GGCC_G
Cuts at: 216 4562 216
Size: 4346 2631
EarI CTCTTCn'nnn_
Cuts at: 278 1162 2423 3131 3260 3731 3863 5000 5210
Size: 884 1261 708 129 471 132 1137 210
Cuts at: 5210 278
Size: 2045
Fragments arranged by size:
2045 1261 1137 884 708 471 210 132
129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5185 5495 5641 6787 5185
Size: 310 146 1146 5375
Fragments arranged by size:
5375 1146 310 146
EcoICRI GAG'CTC
Cuts at: 343 1871 3989 343
Size: 1528 2118 3331
Fragments arranged by size:
3331 2118 1528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 359 1369 1513 2444 3641 4802 5234 5981
Size: 57 1010 144 931 1197 1161 432 747
Cuts at: 5981 6249 302
Size: 268 1030
Fragments arranged by size:
1197 1161 1030 1010 931 747 432 268
144 57
EcoNI CCTnn'n_nnAGG
Cuts at: 1456 1510 2911 1456
Size: 54 1401 5522
Fragments arranged by size:
5522 1401 54
EcoO109I rG'GnC_Cy
Cuts at: 412 4116 412
Size: 3704 3273
EcoRI G'AATT_C
Cuts at: 3981 3981
Size: 6977
EcoRV GAT'ATC
Cuts at: 1629 1629
Size: 6977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1014 2521 2839 3157 4996 1014
Size: 1507 318 318 1839 2995
Fragments arranged by size:
2995 1839 1507 318 318
FspI TGC'GCA
Cuts at: 4758 4758
Size: 6977
HaeII r_GCGC'y
Cuts at: 185 429 4411 4659 5681 6660 185
Size: 244 3982 248 1022 979 502
Fragments arranged by size:
3982 1022 979 502 248 244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 3294 3326 3601 3633 4819 4849 4851
Size: 32 3066 32 275 32 1186 30 2
Cuts at: 4851 4881 196
Size: 30 2292
Fragments arranged by size:
3066 2292 1186 275 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 4016 4016
Size: 6977
HindIII A'AGCT_T
Cuts at: 2225 2225
Size: 6977
KasI G'GCGC_C
Cuts at: 181 4407 4655 181
Size: 4226 248 2503
Fragments arranged by size:
4226 2503 248
KpnI G_GTAC'C
Cuts at: 3997 3997
Size: 6977
MluI A'CGCG_T
Cuts at: 6410 6410
Size: 6977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 714 901 3640 5648 5832 714
Size: 187 2739 2008 184 1859
Fragments arranged by size:
2739 2008 1859 187 184
MscI TGG'CCA
Cuts at: 4738 4738
Size: 6977
MslI CAynn'nnrTG
Cuts at: 704 4966 5093 704
Size: 4262 127 2588
Fragments arranged by size:
4262 2588 127
MspA1I CmG'CkG
Cuts at: 4088 4402 4762 5775 6020 4088
Size: 314 360 1013 245 5045
Fragments arranged by size:
5045 1013 360 314 245
NaeI GCC'GGC
Cuts at: 5158 5158
Size: 6977
NarI GG'CG_CC
Cuts at: 182 4408 4656 182
Size: 4226 248 2503
Fragments arranged by size:
4226 2503 248
NcoI C'CATG_G
Cuts at: 753 753
Size: 6977
NdeI CA'TA_TG
Cuts at: 3477 3477
Size: 6977
NgoMIV G'CCGG_C
Cuts at: 5156 5156
Size: 6977
NspI r_CATG'y
Cuts at: 1327 3124 4030 4530 5437 1327
Size: 1797 906 500 907 2867
Fragments arranged by size:
2867 1797 907 906 500
PciI A'CATG_T
Cuts at: 5433 5433
Size: 6977
PfoI T'CCnGG_A
Cuts at: 1944 1944
Size: 6977
PmeI GTTT'AAAC
Cuts at: 3976 3976
Size: 6977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6116 6148 6116
Size: 32 6945
PpuMI rG'GwC_Cy
Cuts at: 412 412
Size: 6977
PspXI vC'TCGA_Gb
Cuts at: 3589 3589
Size: 6977
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2483 2515 2483
Size: 32 6945
PstI C_TGCA'G
Cuts at: 679 1656 3045 4024 4709 679
Size: 977 1389 979 685 2947
Fragments arranged by size:
2947 1389 979 977 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6977
PvuII CAG'CTG
Cuts at: 4402 4762 4402
Size: 360 6617
RsrII CG'GwC_CG
Cuts at: 5172 5172
Size: 6977
SacI G_AGCT'C
Cuts at: 345 1873 3991 345
Size: 1528 2118 3331
Fragments arranged by size:
3331 2118 1528
SalI G'TCGA_C
Cuts at: 4014 4014
Size: 6977
SapI GCTCTTCn'nnn_
Cuts at: 1162 3131 3731 5000 5210 1162
Size: 1969 600 1269 210 2929
Fragments arranged by size:
2929 1969 1269 600 210
SbfI CC_TGCA'GG
Cuts at: 4024 4024
Size: 6977
ScaI AGT'ACT
Cuts at: 6494 6494
Size: 6977
SfcI C'TryA_G
Cuts at: 33 675 1652 2651 3041 3245 4020 4705 5698
Size: 642 977 999 390 204 775 685 993
Cuts at: 5698 5889 33
Size: 191 1121
Fragments arranged by size:
1121 999 993 977 775 685 642 390
204 191
SfoI GGC'GCC
Cuts at: 183 4409 4657 183
Size: 4226 248 2503
Fragments arranged by size:
4226 2503 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6977
SmaI CCC'GGG
Cuts at: 3999 3999
Size: 6977
SmlI C'TyrA_G
Cuts at: 1453 2803 3001 3589 4132 5047 5539 5801 1453
Size: 1350 198 588 543 915 492 262 2629
Fragments arranged by size:
2629 1350 915 588 543 492 262 198
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6977
SphI G_CATG'C
Cuts at: 4030 4530 4030
Size: 500 6477
SspI AAT'ATT
Cuts at: 865 935 1052 1081 2094 2608 3208 865
Size: 70 117 29 1013 514 600 4634
Fragments arranged by size:
4634 1013 600 514 117 70 29
StyI C'CwwG_G
Cuts at: 753 1037 4111 4465 753
Size: 284 3074 354 3265
Fragments arranged by size:
3265 3074 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 139 2167 4560 4693 5152 5361 139
Size: 2028 2393 133 459 209 1755
Fragments arranged by size:
2393 2028 1755 459 209 133
TatI w'GTAC_w
Cuts at: 580 2499 2643 6492 580
Size: 1919 144 3849 1065
Fragments arranged by size:
3849 1919 1065 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3337 3369 6494 6526 3337
Size: 32 3125 32 3788
Fragments arranged by size:
3788 3125 32 32
Tth111I GACn'n_nGTC
Cuts at: 2926 4774 2926
Size: 1848 5129
XbaI T'CTAG_A
Cuts at: 4008 4008
Size: 6977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4472 4472
Size: 6977
XhoI C'TCGA_G
Cuts at: 1453 3589 1453
Size: 2136 4841
XmaI C'CCGG_G
Cuts at: 3997 3997
Size: 6977
XmnI GAAnn'nnTTC
Cuts at: 638 1136 1298 2397 3485 638
Size: 498 162 1099 1088 4130
Fragments arranged by size:
4130 1099 1088 498 162
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AscI AvrII
BbsI BfrBI BglI BmtI BsaXI BsgI BsiWI BsmBI
BssHII BstEII Bsu36I ClaI CspCI DraIII FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI ZraI